Comprehensive Insulin Receptor Phosphorylation Dynamics Profiled by Mass Spectrometry

FEBS Journal ◽  
2021 ◽  
Author(s):  
Zhongping Liao ◽  
Chen Zhang ◽  
Liyun Ding ◽  
Julie S. Moyers ◽  
Jason X. Tang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Insulin Receptor ◽  
Receptor Phosphorylation

scholarly journals Relationships between urinary inositol excretions and whole-body glucose tolerance and skeletal muscle insulin receptor phosphorylation

Metabolism ◽  
2008 ◽  
Vol 57 (11) ◽  
pp. 1545-1551 ◽  
Author(s):  
April J. Stull ◽  
John P. Thyfault ◽  
Mark D. Haub ◽  
Richard E. Ostlund ◽  
Wayne W. Campbell
Keyword(s):  
Skeletal Muscle ◽  
Glucose Tolerance ◽  
Insulin Receptor ◽  
Whole Body ◽  
Receptor Phosphorylation

Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment

2017 ◽  
Vol 27 (2) ◽  
pp. 120-131 ◽  
Author(s):  
Katarzyna Głombik ◽  
Joanna Ślusarczyk ◽  
Ewa Trojan ◽  
Katarzyna Chamera ◽  
Bogusława Budziszewska ◽  
...  
Keyword(s):  
Drug Treatment ◽  
Insulin Receptor ◽  
Antidepressant Drug ◽  
Receptor Phosphorylation ◽  
Prenatally Stressed ◽  
Antidepressant Drug Treatment ◽  
Insight Into

scholarly journals Role of insulin receptor phosphorylation in the insulinomimetic effects of hydrogen peroxide.

1987 ◽  
Vol 84 (22) ◽  
pp. 8115-8119 ◽  
Author(s):  
G. R. Hayes ◽  
D. H. Lockwood
Keyword(s):  
Hydrogen Peroxide ◽  
Insulin Receptor ◽  
Receptor Phosphorylation

scholarly journals Cold Press Pomegranate Seed Oil Attenuates Dietary-Obesity Induced Hepatic Steatosis and Fibrosis through Antioxidant and Mitochondrial Pathways in Obese Mice

2020 ◽  
Vol 21 (15) ◽  
pp. 5469 ◽  
Author(s):  
Marco Raffaele ◽  
Maria Licari ◽  
Sherif Amin ◽  
Ragin Alex ◽  
Hsin-hsueh Shen ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Metabolic Syndrome ◽  
Insulin Receptor ◽  
Hepatic Fibrosis ◽  
Seed Oil ◽  
Liver Lipid ◽  
Lipid Uptake ◽  
Receptor Phosphorylation ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

Aim: Obesity is associated with metabolic syndrome, hypertension, dyslipidemia, nonalcoholic fatty liver disease (NAFLD), and type 2 diabetes. In this study, we investigated whether the dietary supplementation of pomegranate seed oil (PSO) exerted a protective effect on liver lipid uptake, fibrosis, and mitochondrial function in a mouse model of obesity and insulin resistance. Method: In this in vivo study, eight-week-old C57BL/6J male mice were fed with a high fat diet (HFD) for 24 weeks and then were divided into three groups as follows: group (1) Lean; group (n = 6) (2) HF diet; group (n = 6) (3) HF diet treated with PSO (40 mL/kg food) (n = 6) for eight additional weeks starting at 24 weeks. Physiological parameters, lipid droplet accumulation, inflammatory biomarkers, antioxidant biomarkers, mitochondrial biogenesis, insulin sensitivity, and hepatic fibrosis were determined to examine whether PSO intervention prevents obesity-associated metabolic syndrome. Results: The PSO group displayed an increase in oxygen consumption, as well as a decrease in fasting glucose and blood pressure (p < 0.05) when compared to the HFD-fed mice group. PSO increased both the activity and expression of hepatic HO-1, downregulated inflammatory adipokines, and decreased hepatic fibrosis. PSO increased the levels of thermogenic genes, mitochondrial signaling, and lipid metabolism through increases in Mfn2, OPA-1, PRDM 16, and PGC1α. Furthermore, PSO upregulated obesity-mediated hepatic insulin receptor phosphorylation Tyr-972, p-IRB tyr1146, and pAMPK, thereby decreasing insulin resistance. Conclusions: These results indicated that PSO decreased obesity-mediated insulin resistance and the progression of hepatic fibrosis through an improved liver signaling, as manifested by increased insulin receptor phosphorylation and thermogenic genes. Furthermore, our findings indicate a potential therapeutic role for PSO in the prevention of obesity-associated NAFLD, NASH, and other metabolic disorders.

scholarly journals Phorbol esters modulate insulin receptor phosphorylation and insulin action in cultured hepatoma cells.

1984 ◽  
Vol 81 (24) ◽  
pp. 7797-7801 ◽  
Author(s):  
S. Takayama ◽  
M. F. White ◽  
V. Lauris ◽  
C. R. Kahn
Keyword(s):  
Insulin Receptor ◽  
Insulin Action ◽  
Phorbol Esters ◽  
Hepatoma Cells ◽  
Receptor Phosphorylation

scholarly journals Calcium-dependence of insulin receptor phosphorylation

1983 ◽  
Vol 214 (2) ◽  
pp. 361-366 ◽  
Author(s):  
W E Plehwe ◽  
P F Williams ◽  
I D Caterson ◽  
L C Harrison ◽  
J R Turtle
Keyword(s):  
Membrane Protein ◽  
Insulin Receptor ◽  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Beta Subunit ◽  
Receptor Phosphorylation ◽  
32P Incorporation ◽  
Isolated Rat

Phosphorylation of the insulin receptor of isolated rat adipocytes in response to insulin has been studied. Immunoprecipitation of adipocyte membrane protein demonstrated increased incorporation of 32P after exposure to insulin for 15 min, but this was dependent on the presence of physiological concentrations of Ca2+ and Mg2+. Autoradiography of solubilized immunoprecipitated membrane protein after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed that most of the 32P incorporation occurred in a band corresponding to Mr 95 000, which has been identified previously as the beta-subunit of the insulin receptor. 32P incorporation was inhibited by 2,4-dinitrophenol and trifluoperazine. It is suggested that insulin-receptor phosphorylation is an energy-requiring process that is Ca2+-dependent and may be modulated by calmodulin. Phosphorylation may proceed independently of glucose transport.

The Effect of Protein Kinase-C Inhibition on Insulin Receptor Phosphorylation

Endocrinology ◽  
1990 ◽  
Vol 127 (1) ◽  
pp. 481-487 ◽  
Author(s):  
VINCENT DURONIO ◽  
STEVEN JACOBS
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Insulin Receptor ◽  
Receptor Phosphorylation ◽  
Kinase C
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