A pilot examination of the fermentation products, aerobic stability and bacterial community of total mixed ration silage produced in Vietnam

2014 ◽  
Vol 60 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Tu Tran Thi Minh ◽  
Van Nguyen Huu ◽  
Naoki Nishino
Keyword(s):  
Bacterial Community ◽  
Fermentation Products ◽  
Total Mixed Ration ◽  
Aerobic Stability

scholarly journals Silage Fermentation, Bacterial Community, and Aerobic Stability of Total Mixed Ration Containing Wet Corn Gluten Feed and Corn Stover Prepared with Different Additives

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1775
Author(s):  
Guangning Zhang ◽  
Xinpeng Fang ◽  
Guanzhi Feng ◽  
Yang Li ◽  
Yonggen Zhang
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
Corn Stover ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Corn Gluten ◽  
Corn Gluten Feed ◽  
Fermentation Quality ◽  
Wet Corn Gluten Feed ◽  
Gluten Feed

The objective of this study was to investigate the effects of different additives on the fermentation quality, bacterial community, and aerobic stability of total mixed ration (TMR) silage containing wet corn gluten feed (WCGF) and corn stover. The TMR was ensiled with four treatments: (1) no additive (control); (2) lactic acid bacteria (LAB); (3) fibrolytic enzyme (EN); (4) LAB + EN. The EN and LAB + EN decreased the neutral detergent fiber and acid detergent fiber contents. Additives led to a higher lactic acid (LA) content (p < 0.0001) compared to control at all ensiling times. Silages inoculated with LAB and LAB + EN had higher dry matter (p = 0.0007), LA (p < 0.0001) and acetic acid (AA) contents (p < 0.0001) compared to control. The LAB and LAB + EN had significantly lowest ammonia nitrogen among the treatments, while no significant difference occurred after days 7 of ensiling. Silages treated with LAB and LAB + EN had a higher LAB count (p < 0.0001) and a lower pH, yeast, and mold counts compared to other silages. The LAB and LAB + EN greatly increased the portions of Firmicutes and Lactobacillus (p < 0.0001, and p < 0.0001, respectively) and reduced undesirable bacteria. Inoculation with LAB + EN and LAB improved aerobic stability of TMR silages indicated by higher and more stable LA and AA contents, smaller rise in pH, and yeast count than other silages. The LAB + EN and LAB reduced microbial diversity and improved the fermentation quality and aerobic stability of TMR silage containing WCGF and corn stover.

scholarly journals Changing Dietary Calcium-Phosphorus Level and Cereal Source Selectively Alters Abundance of Bacteria and Metabolites in the Upper Gastrointestinal Tracts of Weaned Pigs

2013 ◽  
Vol 79 (23) ◽  
pp. 7264-7272 ◽  
Author(s):  
Barbara U. Metzler-Zebeli ◽  
Evelyne Mann ◽  
Stephan Schmitz-Esser ◽  
Martin Wagner ◽  
Mathias Ritzmann ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gut Health ◽  
Weaned Pigs ◽  
Fermentation Products ◽  
Content Type ◽  
Young Pigs ◽  
Bacterial Microbiota ◽  
Calcium Phosphorus ◽  
And Performance ◽  
The Stability

ABSTRACTSeveral dietary ingredients may affect the bacterial community structure and metabolism in the porcine gut and may therefore influence animals' health and performance. This study investigated the effects of cereal source and calcium-phosphorus (CaP) level in the diet on bacterial microbiota and metabolites, nutrient intake, and gut environment in weaned pigs. Pigs (n= 8/treatment) were fed wheat-barley- or corn-based diets with an adequate or high CaP level for 14 days. Effects on microbiota in the stomach, ileum, and midcolon were assessed using quantitative PCR. Data showed thatEnterobacteriaceae,Campylobacterspp., andHelicobacterspp., which all contain highly immune reactive lipopolysaccharide (LPS), were abundant at all gut sites. Diet effects on bacteria and metabolites were moderate and occurred mainly in the upper gut, whereas no effects on bacteria, fermentation products, and LPS could be observed in the colon. Differences in carbohydrate intake with corn versus wheat-barley diets selectively stimulatedBifidobacteriumin the stomach and ileum. There was a growth advantage for a few bacterial groups in the stomach and ileum of pigs fed the high versus adequate CaP level (i.e., gastricEnterobacteriaceaeand ilealEnterococcus,Bacteroides-Prevotella-Porphyromonas, andCampylobacter). Interestingly, gastrointestinal pH was not affected by dietary CaP level. The present findings demonstrate the stability of the bacterial community and gut environment toward dietary changes even in young pigs. The results on stimulation of gastric and ilealBifidobacteriumby corn diets may be employed in nutritional strategies to support gut health after weaning.

Fermentation profile, microbial populations, taxonomic diversity and aerobic stability of total mixed ration silages based on Cactus and Gliricidia

2020 ◽  
Vol 158 (5) ◽  
pp. 396-405
Author(s):  
F. N. S. Santos ◽  
E. M. Santos ◽  
J. S. Oliveira ◽  
G. R. Medeiros ◽  
A. M. Zanine ◽  
...  
Keyword(s):  
Interaction Effect ◽  
Diversity Index ◽  
Taxonomic Diversity ◽  
Gliricidia Sepium ◽  
Microbial Populations ◽  
Total Mixed Ration ◽  
Bacterial Phyla ◽  
Aerobic Stability ◽  
Significant Difference ◽  
Fermentation Profile

AbstractThe aim of this study was to investigate the effects of Cactus (Opuntia spp) levels in total mixed ration silages based on Cactus and Gliricidia (Gliricidia sepium (Jacq.) Steud) on the fermentation profile, microbial populations, aerobic stability and taxonomic diversity. The completely randomized design was used in a 4 × 4 factorial design with four replications, being four rations with different levels of Cactus (15, 30, 45, 60% based on the dry matter) and four opening periods (0, 15, 30 and 60 days of fermentation). An interaction effect (P < 0.050) was observed among the diets and opening times for mould and yeast populations. An interaction effect for the levels of acetic acid was observed, where the diets 15, 30, 45 and 60% showed higher values at 60 days (0.44, 0.41, 0.35 and 0.40 g/kg DM, respectively). A significant difference was observed for the richness and diversity index (Chao1 and Shannon). The most abundant bacterial phyla were Proteobacteria and Firmicutes and the genera Lactobacillus and Weissella. Cactus can be added in total mixed ration silages up to the level of 60% in a way that it positively affects the qualitative indicators of the silages, modulating the taxonomic communities and allowing the predominance of important groups for preservation of the ensiled mass.

scholarly journals Aerobic Stability and Effects of Yeasts during Deterioration of Non-fermented and Fermented Total Mixed Ration with Different Moisture Levels

2015 ◽  
Vol 28 (6) ◽  
pp. 816-826 ◽  
Author(s):  
W. Hao ◽  
H. L. Wang ◽  
T. T. Ning ◽  
F. Y. Yang ◽  
C. C. Xu
Keyword(s):  
Total Mixed Ration ◽  
Aerobic Stability

Fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages prepared with whole-plant corn (Zea mays L.) and hulless barley (Hordeum vulgare L.) straw

2018 ◽  
Vol 58 (10) ◽  
pp. 1860 ◽  
Author(s):  
XianJun Yuan ◽  
AiYou Wen ◽  
Jian Wang ◽  
JunFeng Li ◽  
Seare T. Desta ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Ammonia Nitrogen ◽  
Stability Test ◽  
Small Scale ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was carried out to assess the effects of adding Lactobacillus plantarum, molasses or/and ethanol on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration (TMR) silage, which is well accepted in small-scale dairy farms in Tibet. Total mixed ration were ensiled in laboratory silos (1 L) and treated with (1) no additive (Control), (2) ethanol (E, 25 ml/kg fresh weight (FW)), (3) molasses (M, 30 g/kg FW); (4) Lactobacillus plantarum (L, 106cfu/g FW); (5) ethanol + molasses (EM); and (6) ethanol + Lactobacillus plantarum (EL). After 45 days of ensiling, six silos per treatment were opened for the fermentation quality and in vitro digestibility analyses, whereas 18 silos were used for the aerobic stability test for the following 9 days. All TMR silages were well preserved with dominant lactic acid (LA), low pH and ammonia nitrogen, and negligible propionic and butyric acid. The L and EL silages had the lowest pH and highest LA concentrations. The addition of ethanol did not inhibit silage fermentation as there were no significant differences for the pH, LA, acetic acid, negligible propionic acid or ammonia nitrogen content, lactic acid bacteria and yeast counts between Control and the E silage. During the aerobic stability test, pH increased by 1.39, 1.67, 1.69 and 0.74 for the Control, M, L and EM silages, but only 0.40 and 0.34 for E and EL silages, respectively. Upon exposure to air, the LA concentration in the L silage was evidently (P < 0.05) decreased, whereas LA concentration in the EL silage remained the highest value after the third day of aerobic exposure. Mean populations of aerobic bacteria and yeast in the E and EL silages were lower (P < 0.05) than those of the Control. These findings suggested that L. plantarum is effective in improving fermentation quality of TMR silages. Although the addition of ethanol in our study did not depress the fermentation of the TMR silages, it showed potential to inhibit the aerobic spoilage of TMR silages, either alone or in combination with the L. plantarum. It is concluded that L. plantarum combined with ethanol not only ensures better fermentation but also could improve aerobic stability.

Ensiling fermentation products and aerobic stability of corn and sorghum silages

2011 ◽  
Vol 57 (1) ◽  
pp. 46-50 ◽  
Author(s):  
Zwi G. Weinberg ◽  
Prabhat Khanal ◽  
Cihat Yildiz ◽  
Yaira Chen ◽  
Amichay Arieli
Keyword(s):  
Fermentation Products ◽  
Aerobic Stability

scholarly journals Bromochloromethane, a Methane Analogue, Affects the Microbiota and Metabolic Profiles of the Rat Gastrointestinal Tract

2015 ◽  
Vol 82 (3) ◽  
pp. 778-787 ◽  
Author(s):  
Yu-Xiang Yang ◽  
Chun-Long Mu ◽  
Zhen Luo ◽  
Wei-Yun Zhu
Keyword(s):  
Bacterial Community ◽  
Bacterial Community Composition ◽  
Wistar Rat ◽  
Animal Production ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Metabolic Profiles ◽  
Fermentation Products ◽  
Bacterial Fermentation ◽  
Male Wistar Rats

ABSTRACTBromochloromethane (BCM), an inhibitor of methanogenesis, has been used in animal production. However, little is known about its impact on the intestinal microbiota and metabolic patterns. The present study aimed to investigate the effect of BCM on the colonic bacterial community and metabolism by establishing a Wistar rat model. Twenty male Wistar rats were randomly divided into two groups (control and treated with BCM) and raised for 6 weeks. Bacterial fermentation products in the cecum were determined, and colonic methanogens and sulfate-reducing bacteria (SRB) were quantified. The colonic microbiota was analyzed by pyrosequencing of the 16S rRNA genes, and metabolites were profiled by gas chromatography and mass spectrometry. The results showed that BCM did not affect body weight and feed intake, but it did significantly change the intestinal metabolic profiles. Cecal protein fermentation was enhanced by BCM, as methylamine, putrescine, phenylethylamine, tyramine, and skatole were significantly increased. Colonic fatty acid and carbohydrate concentrations were significantly decreased, indicating the perturbation of lipid and carbohydrate metabolism by BCM. BCM treatment decreased the abundance of methanogen populations, while SRB were increased in the colon. BCM did not affect the total colonic bacterial counts but significantly altered the bacterial community composition by decreasing the abundance of actinobacteria, acidobacteria, and proteobacteria. The results demonstrated that BCM treatment significantly altered the microbiotic and metabolite profiles in the intestines, which may provide further information on the use of BCM in animal production.

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