scholarly journals An In Vivo Model of Wound Healing in Genetically Modified Skin-Humanized Mice

2004 ◽  
Vol 123 (6) ◽  
pp. 1182-1191 ◽  
Author(s):  
María José Escámez ◽  
Marta García ◽  
Fernando Larcher ◽  
Alvaro Meana ◽  
Evangelina Muñoz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Genetically Modified ◽  
Humanized Mice ◽  
In Vivo Model

scholarly journals Immunohistological changes in skin wounds during the early periods of healing in a rat model

2012 ◽  
Vol 57 (No. 2) ◽  
pp. 77-82 ◽  
Author(s):  
F. Sabol ◽  
L. Dancakova ◽  
P. Gal ◽  
T. Vasilenko ◽  
M. Novotny ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wide Spectrum ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Skin Wound ◽  
Hair Follicles ◽  
In Vivo Model ◽  
Wound Healing Process ◽  
Rat Skin

The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin 14, α-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

Macrophages Prevent Human Red Blood Cell Reconstitution In NOD/SCID and NOD/SCID/γc−/− Mice.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 3723-3723
Author(s):  
Zheng Hu ◽  
Yong-Guang Yang
Keyword(s):  
Mononuclear Cells ◽  
Conflicts Of Interest ◽  
Embryonic Stem ◽  
Humanized Mice ◽  
In Vivo Model ◽  
Recipient Mouse ◽  
Hematopoietic Stem ◽  
Immunodeficient Mice ◽  
Human Rbcs

Abstract Abstract 3723 An animal model supporting human erythropoiesis will be highly valuable for assessing the biological function of human RBCs under physiological and disease settings, and for evaluating protocols of in vitro RBC differentiation from human embryonic stem cells. Although immunodeficient mice on the NOD background have been widely used to study human hematopoietic stem cell function in vivo, the successful use of these mice in the study of human erythropoiesis and RBC function has not been reported. We have previously shown that co-transplantation of human fetal thymic tissue (under renal capsule) and CD34+ fetal liver cells (FLCs; i.v.) in NOD/SCID or NOD/SCID/γc−/− mice results in the development of multilineage human hematopoietic cells. Here, we analyzed human RBC reconstitution in these humanized mice. Although a large number of human erythrocytes, which consisted predominantly of immature nucleated erythrocytes, were detected in the bone marrow of human fetal thymus/CD34+ FLC-grafted mice, human RBCs were undetectable in blood of these mice, even in those with nearly full human chimerism in peripheral blood mononuclear cells (PBMCs). Recipient mouse macrophage-mediated rejection is, at least, one of the major mechanisms responsible for the lack of human RBCs in these mice, as human RBCs became detectable in blood following macrophage depletion and disappeared again after withdrawal of treatment. Furthermore, treatment with human erythropoietin (EPO) and human IL-3 significantly increased human RBC reconstitution in mice that were depleted of macrophages. Like the human RBCs developed in the humanized mice, exogenously injected normal human RBCs were also rapidly rejected by macrophages in NOD/SCID mice. Taken together, our data demonstrate that human RBCs are highly susceptible to rejection by macrophages in immunodeficient mice. Thus, strategies for preventing human RBC rejection by macrophages are required for using immunodeficient mice as an in vivo model to study human erythropoiesis and RBC function. Disclosures: No relevant conflicts of interest to declare.

Full-Thickness Human Foreskin Transplantation onto Nude Rats as an In Vivo Model of Acute Human Wound Healing

2003 ◽  
Vol 111 (6) ◽  
pp. 1988-1997 ◽  
Author(s):  
Peter B. Petratos ◽  
Jie Chen ◽  
Robert A. Soslow ◽  
Clifford B. Bleustein ◽  
Diane Felsen ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Model ◽  
Full Thickness ◽  
Nude Rats

A novel in vivo model for predicting myelotoxicity of chemotherapeutic agents using IL-3/GM-CSF transgenic humanized mice

2017 ◽  
Vol 281 ◽  
pp. 152-157 ◽  
Author(s):  
R. Ito ◽  
D. Nagai ◽  
N. Igo ◽  
Y. Okuda ◽  
K. Sekine ◽  
...  
Keyword(s):  
Chemotherapeutic Agents ◽  
Humanized Mice ◽  
In Vivo Model ◽  
Gm Csf

Effects of amniotic membrane extract and deferoxamine on angiogenesis in wound healing: an in vivo model

2018 ◽  
Vol 27 (Sup6) ◽  
pp. S26-S32 ◽  
Author(s):  
Ramyar Farzan ◽  
Mahsa Moeinian ◽  
Alireza Abdollahi ◽  
Zahra Jahangard-Rafsanjani ◽  
Abbas Alipour ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
In Vivo Model ◽  
Membrane Extract

scholarly journals Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice

2019 ◽  
Vol 2 (2) ◽  
pp. e201800195 ◽  
Author(s):  
Masashi Matsuda ◽  
Rintaro Ono ◽  
Tomonori Iyoda ◽  
Takaho Endo ◽  
Makoto Iwasaki ◽  
...  
Keyword(s):  
Nk Cells ◽  
Immune Cells ◽  
Nk Cell ◽  
Cell Development ◽  
Innate Immune ◽  
Humanized Mice ◽  
In Vivo Model ◽  
Hematopoietic Stem ◽  
Nsg Mice

The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells.

Development of a novel, highly quantitative in vivo model for the study of biofilm-impaired cutaneous wound healing

2011 ◽  
Vol 19 (3) ◽  
pp. 400-410 ◽  
Author(s):  
Anandev N. Gurjala ◽  
Matthew R. Geringer ◽  
Akhil K. Seth ◽  
Seok J. Hong ◽  
Mark S. Smeltzer ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Model ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

scholarly journals Cellular HIV Reservoirs and Viral Rebound from the Lymphoid Compartments of 4′-Ethynyl-2-Fluoro-2′-Deoxyadenosine (EFdA)-Suppressed Humanized Mice

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 256 ◽  
Author(s):  
Ekaterina Maidji ◽  
Mary Moreno ◽  
Jose Rivera ◽  
Pheroze Joshi ◽  
Sofiya Galkina ◽  
...  
Keyword(s):  
Transcriptase Inhibitor ◽  
Humanized Mice ◽  
In Vivo Model ◽  
Lymphoid Tissues ◽  
Viral Rebound ◽  
Hiv Reverse Transcriptase ◽  
Hiv Reservoirs ◽  
Hiv Reservoir ◽  
Drug Cessation

Although antiretroviral therapy (ART) greatly suppresses HIV replication, lymphoid tissues remain a sanctuary site where the virus may replicate. Tracking the earliest steps of HIV spread from these cellular reservoirs after drug cessation is pivotal for elucidating how infection can be prevented. In this study, we developed an in vivo model of HIV persistence in which viral replication in the lymphoid compartments of humanized mice was inhibited by the HIV reverse transcriptase inhibitor 4′-ethynyl-2-fluoro-2′-deoxyadenosine (EFdA) to very low levels, which recapitulated ART-suppression in HIV-infected individuals. Using a combination of RNAscope in situ hybridization (ISH) and immunohistochemistry (IHC), we quantitatively investigated the distribution of HIV in the lymphoid tissues of humanized mice during active infection, EFdA suppression, and after drug cessation. The lymphoid compartments of EFdA-suppressed humanized mice harbored very rare transcription/translation-competent HIV reservoirs that enable viral rebound. Our data provided the visualization and direct measurement of the early steps of HIV reservoir expansion within anatomically intact lymphoid tissues soon after EFdA cessation and suggest a strategy to enhance therapeutic approaches aimed at eliminating the HIV reservoir.

scholarly journals Polysaccharides as wound healing agent: a mini review

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 31-37
Author(s):  
J. Rohini ◽  
M.F. Wan Ezumi ◽  
M.S. Rabeta
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Scientific Evidence ◽  
Well Being ◽  
In Vivo Model ◽  
Current Trends ◽  
Healing Agent ◽  
Pharmaceutical Industries

Medicinal eukaryotes, such as plants and fungi, have prompted researchers to conduct extensive studies on their medicinal values for drug discovery. Current trends focus on bioactive compounds of medicinal plants to produce inventions in the medical and health fields. Among many bioactive compounds, polysaccharides attract attention because they are non-toxic and have no side effects. Polysaccharides have been widely used in food and pharmaceutical industries as a secondary ingredient for several decades. This paper reviewed the applications of polysaccharides as wound healing agents. Wounds can affect the patient’s well-being, self-image, working capacity and independence. Research studies on different sources of polysaccharides by in vitro and in vivo model have been investigated. Based on the scientific evidence related to polysaccharides, this work will be a baseline study for future investigations in different fields. All literature was accessed through available electronic databases

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