Relationship between Canine Mucosal and Serum Immunoglobulin A (IgA) Concentrations: Serum IgA Does Not Assess Duodenal Secretory IgA

The objective: the study of immunomodulatory effects of a probiotic, which contains lyophilized Lactobacillus (Lactobacillus rhamnosus) – 13 mg (2,0ґ109 CFU) and lyophilized bifidobacteria (Bifidobacterium lactis) – 4 mg (2,0ґ109 CFU) the level of serum immunoglobulin IgA as a marker of local immunity in the plasma of women of reproductive age with the violation of the biocenosis of the vagina. Patients and methods. The study involved 86 patients of reproductive age with the violation of the vaginal biocenosis, which were divided into two groups according to received treatment. A survey was conducted for all patients in both groups: determine the level of serum IgA, measuring pH of vaginal environment and the quantification of lactobacilli and pathogenic flora with the help of test-system «Florotsenoz» before treatment and in 6 weeks after treatment. The state of vaginal microbiocenosis in both groups before treatment was homogeneous. Patients in both groups as therapy at the first stage of treatment received, if necessary antimicrobial therapy depending on the selected flora. In the second stage (restoration of microflora) patient of the main group received systemic probiotic combined with a complex prebiotic local action, patients in the control group, the probiotic localy in the form of the vaginal candles or tablets. Results. The research stated the increasing level of serum IgA in blood plasma of patients of the main group compared to control group at 20%, normalizing the pH of the vaginal environment in the main group in 94% of cases, which indicates an increase of immunity in mucosal. Conclusion. The inclusion of the systemic probiotic in the scheme of treatment of disorders of biocenosis of the vagina system enhances the increasing of immunity of the mucous membranes, and the vaginal tablets prebiotic of local action restores the own normal microflora of the vagina. Key words: serum immunoglobulin A, local immunity, vaginal dysbiosis, probiotics, prebiotics, vaginal microbiocenosis, the pH of the vaginal environment.

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Secretory Immunoglobulin a in Oral and Respiratory Passages in Man

Annals of Otology Rhinology & Laryngology ◽

10.1177/0003489475084s2001 ◽

1975 ◽

Vol 84 (20_suppl) ◽

pp. 1-23 ◽

Cited By ~ 13

Author(s):

Goro Mogi

Keyword(s):

Immunoglobulin A ◽

Secretory Component ◽

Secretory Iga ◽

Secretory Cells ◽

Antigenic Relationship ◽

Serum Iga ◽

Human Colostrum ◽

Specific Antisera ◽

Antigenic Relationships ◽

Secretory Immunoglobulin

Secretory IgA (SIgA) is the predominant immunoglobulin in certain external secretions and may have an important role in immunological mucosal resistance. SIgA differs in chemical and immunological properties from serum IgA. The present study was undertaken to investigate the antigenic relationship between SIgA, free secretory component (FSC) and serum IgA and the localization of SIgA as well as other immunological classes in tissues of oral and respiratory passages by use of immunofluorescence technique. SIgA and FSC were highly purified from human colostrum and rabbit anti-SIgA and anti-SC antisera were prepared. On the basis of antigenic relationships between SIgA, FSC and serum IgA, it was emphasized that individual specific antisera for SC and IgA and/or SIgA should be used in immunochemical or immunohistological investigations for SIgA. The present study failed to detect SC determinants in palatine and lingual tonsils. However, it was evident that cells present in the pharyngeal tonsillar epithelium contain SC determinants. SC molecules may be synthesized in certain secretory cells of mucous membrane and glandular epithelium and the combining of SC with IgA could occur in the cytoplasm of epithelial cells, the intercellular spaces and/or in the lumens of glandular acini and ductules.

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Major Immunoglobulin Ratios in Carcinoma of the Head and Neck

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348947808700323 ◽

1978 ◽

Vol 87 (3) ◽

pp. 412-415 ◽

Cited By ~ 11

Author(s):

Arnold E. Katz ◽

John O. Nysather ◽

Lee A. Harker

Keyword(s):

Head And Neck ◽

Immunoglobulin G ◽

Immunoglobulin A ◽

Elevated Serum ◽

Immunoglobulin M ◽

Serum Immunoglobulin ◽

Control Groups ◽

Immunoglobulin Production ◽

Patients With Cancer ◽

Serum Iga

— Serum immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) levels were determined on 245 patients with carcinoma of the head and neck and on 92 controls. Ratios of these levels were calculated for each subject. The patients with cancer demonstrated elevated serum IgA levels ( P <.0001) and elevated IgA/IgM and IgA/IgG ratios ( P <.05). No differences were noted when the IgM/IgG ratios were compared between the cancer and the control groups. These observations are offered as evidence that previously reported elevations of serum IgA levels in patients with carcinoma of the head and neck are not merely an index of nonspecific increased immunoglobulin production in these patients.

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Protection of the Villus Epithelial Cells of the Small Intestine from Rotavirus Infection Does Not Require Immunoglobulin A

Journal of Virology ◽

10.1128/jvi.74.9.4102-4109.2000 ◽

2000 ◽

Vol 74 (9) ◽

pp. 4102-4109 ◽

Cited By ~ 59

Author(s):

Christine M. O'Neal ◽

Gregory R. Harriman ◽

Margaret E. Conner

Keyword(s):

T Cells ◽

Normal Control ◽

Knockout Mice ◽

Immunoglobulin A ◽

Primary Immune Response ◽

Secretory Iga ◽

Compensatory Mechanisms ◽

Rotavirus Infection ◽

Serum Iga ◽

Mucosal Pathogens

ABSTRACT Immunoglobulin A (IgA) is the primary immune response induced in the intestine by rotavirus infection, but vaccination with virus-like particles induces predominantly IgG, not IgA. To definitively assess the role of IgA in protection from rotavirus infection, IgA knockout mice, which are devoid of serum and secretory IgA, were infected and then rechallenged with murine rotavirus at either 6 weeks or 10 months. Following primary rotavirus infection, IgA knockout mice cleared virus as effectively as IgA normal control mice. Rotavirus-infected IgA knockout mice produced no serum or fecal IgA but did have high levels of antirotavirus serum IgG and IgM and fecal IgG, whereas IgA normal control mice made both serum IgA and IgG and fecal IgA. Both IgA normal and IgA knockout mice were totally protected from rotavirus challenge at 42 days. Ten months following a primary infection, both IgA normal and knockout mice still had high levels of serum and fecal antirotavirus antibody and were totally protected from rotavirus challenge. To determine if compensatory mechanisms other than IgG were responsible for protection from rotavirus infection in IgA knockout mice, mice were depleted of CD4+ T cells or CD8+ T cells. No changes in the level of protection were seen in depleted mice. These data show that fecal or systemic IgA is not essential for protection from rotavirus infection and suggest that in the absence of IgA, IgG may play a significant role in protection from mucosal pathogens.

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Evaluation of Systemic and Secretory IgA Concentrations and Immunohistochemical Stains for IgA-Containing B Cells in Mucosal Tissues of an Irish Setter With Selective IgA Deficiency

Journal of the American Animal Hospital Association ◽

10.5326/0390247 ◽

2003 ◽

Vol 39 (3) ◽

pp. 247-250 ◽

Cited By ~ 6

Author(s):

Carol R. Norris ◽

Laurel J. Gershwin

Keyword(s):

B Cells ◽

Immunoglobulin A ◽

Elevated Serum ◽

Lavage Fluid ◽

Immunoglobulin M ◽

Secretory Iga ◽

Poor Correlation ◽

Selective Iga Deficiency ◽

Serum Iga ◽

Immunohistochemical Stains

Immunoglobulin A is the predominant secretory antibody at mucosal surfaces. In the dog, immunoglobulin A deficiency (IgAD) is characterized by low to absent serum IgA and normal to elevated serum immunoglobulin G (IgG) and immunoglobulin M (IgM) concentrations. However, studies comparing serum and secretory IgA in dogs have often documented a poor correlation, suggesting that serum concentrations should not be used to estimate mucosal secretion of this antibody. This report demonstrates the concurrent use of serum IgA, IgG, and IgM; secretory IgA (from bronchoalveolar lavage fluid); and immunohistochemical stains on bronchial and duodenal mucosa for IgA-containing B cells in a young Irish setter with recurrent respiratory and gastrointestinal signs.

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Potent Neutralizing Serum Immunoglobulin A (IgA) in Human Immunodeficiency Virus Type 2-Exposed IgG-Seronegative Individuals

Journal of Virology ◽

10.1128/jvi.78.13.7016-7022.2004 ◽

2004 ◽

Vol 78 (13) ◽

pp. 7016-7022 ◽

Cited By ~ 16

Author(s):

Qin Lizeng ◽

Charlotta Nilsson ◽

Samer Sourial ◽

Sören Andersson ◽

Olav Larsen ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Immunoglobulin A ◽

Serum Immunoglobulin ◽

Serum Samples ◽

Serum Iga ◽

Immunodeficiency Virus ◽

Negative Controls

ABSTRACT The mechanisms behind the resistance to human immunodeficiency virus type 2 (HIV-2) infection are still not fully understood. In the present study, we explored the HIV-2-specific humoral serum immunoglobulin A (IgA) immune response in HIV-2-exposed IgG-seronegative (EGSN) individuals. Serum samples from heterosexual EGSN individuals and their known HIV-2-infected partners, as well as controls originating from Guinea-Bissau in Africa, were studied. Antibody reactivity to native and recombinant envelope glycoproteins was investigated, and the capacity of purified serum IgA to neutralize HIV-2SBL6669 was tested. Our results showed that 16 of 25 EGSN samples exhibited reactivity against whole HIV-2 antigen, 6 of 25 samples reacted with recombinant gp36 (rgp36), and 3 of 25 samples were positive against HIV-2 rgp105; no reactivity to native HIV-2 gp125 was detected. Purified serum IgA antibodies from both EGSN and HIV-2-positive individuals, but not that from the negative controls, exhibited neutralization of HIV-2SBL6669. The most potent neutralization activity was exhibited by IgA purified from EGSN compared to infected individuals' IgA. The antigenic pattern of the HIV-2-positive partners showed that all serum IgA samples were reactive to whole HIV-2 antigen, and 14 of 15 reacted with rgp36. For rgp105 and gp125, 5 of 15 and 4 of 15 samples exhibited binding, respectively. The serum of the EGSN group had a higher mean IgA concentration than that of the negative controls (P < 0.05). Thus, we describe HIV-2-specific serum IgA antigen reactivity and show a more potent serum IgA-mediated HIV-2-neutralizing activity in EGSN individuals than in HIV-2-infected patients.

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The Effects of Secretory IgA in the Mucosal Immune System

BioMed Research International ◽

10.1155/2020/2032057 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

Yue Li ◽

Liang Jin ◽

Tongxin Chen

Keyword(s):

Immune System ◽

Critical Role ◽

Immunoglobulin A ◽

Secretory Component ◽

Mucosal Immune System ◽

Secretory Iga ◽

Antibody Isotype ◽

Serum Iga ◽

Important Field ◽

Immune Exclusion

Immunoglobulin A (IgA) is the most abundant antibody isotype in the mucosal immune system. Structurally, IgA in the mucosal surface is a polymeric structure, while serum IgA is monomeric. Secretory IgA (sIgA) is one of the polymeric IgAs composed of dimeric IgA, J chain, and secretory component (SC). Most of sIgAs were generated by gut and have effects in situ. Besides the function of “immune exclusion,” a nonspecific immune role, recent studies found it also played an important role in the specific immunity and immunoregulation. Thanks to the critical role of sIgA during the mucosal immune system homeostasis between commensal microorganisms and pathogens; it has been an important field exploring the relationship between sIgA and commensal microorganisms.

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Analysis of Immunoglobulin A Antibodies toHelicobacter pylori in Serum and Gastric Juice in Relation to Mucosal Inflammation

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.5.5.617-621.1998 ◽

1998 ◽

Vol 5 (5) ◽

pp. 617-621 ◽

Cited By ~ 15

Author(s):

Shunji Hayashi ◽

Toshiro Sugiyama ◽

Kenji Yokota ◽

Hiroshi Isogai ◽

Emiko Isogai ◽

...

Keyword(s):

Gastric Juice ◽

Immunoglobulin A ◽

Histologic Grade ◽

Secretory Iga ◽

Mucosal Inflammation ◽

Antibody Concentration ◽

Α Subunit ◽

Serum Iga ◽

Iga Antibodies ◽

H Pylori

ABSTRACT Helicobacter pylori is a major etiologic agent in gastroduodenal disorders. In this study, immunoglobulin A (IgA) antibodies to H. pylori antigens were evaluated in serum and gastric juice specimens obtained from patients with gastritis or peptic ulcers by utilizing antibody capture enzyme-linked immunosorbent assays (ACELISAs). Urease α subunit (UA), urease β subunit (UB), the 66-kDa heat shock protein (HSP), and the 25-kDa protein (25K) were used as antigens for the ACELISAs. The antibody titers of the ACELISAs reflect the ratio of H. pylori-specific IgA to total IgA. The ratio is stable, although the antibody concentration fluctuates in gastric juice. By using ACELISAs it was possible to evaluate quantitatively not only serum IgA antibodies but also gastric juice secretory IgA (S-IgA) antibodies. In both serum IgA and gastric juice S-IgA ACELISAs, the titers of antibody to HSP and 25K were remarkably correlated with the histologic grade of gastritis, whereas those to UA and UB were not strongly correlated with histologic grade. Thus, it is useful for estimating the histologic grade of gastritis to quantify serum IgA and gastric juice S-IgA antibodies to HSP and 25K.

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Effect of Concomitant Antibiotic and Vaccine Administration on Serologic Responses to Rotavirus Vaccine

Journal of the Pediatric Infectious Diseases Society ◽

10.1093/jpids/piz044 ◽

2019 ◽

Vol 9 (4) ◽

pp. 479-482

Author(s):

Evan J Anderson ◽

Benjamin Lopman ◽

Jumi Yi ◽

Romina Libster ◽

C Buddy Creech ◽

...

Keyword(s):

Immunoglobulin A ◽

Serum Immunoglobulin ◽

Rotavirus Vaccine ◽

Antibiotic Exposure ◽

Vaccine Administration ◽

Vaccine Study ◽

Serum Iga

Abstract Data from 1174 infants enrolled in a previous rotavirus vaccine study were analyzed to determine the effect of antibiotic exposure (from 14 days before to 7 days after vaccination) on rotavirus serum immunoglobulin A (IgA) responses. Serum IgA responses 1 month after the completion of vaccination were similar among antibiotic-exposed and nonexposed infants.

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Secretory Immunoglobulin a in Oral and Respiratory Passages in Man

Annals of Otology Rhinology & Laryngology ◽

10.1177/00034894750840s301 ◽

1975 ◽

Vol 84 (3_suppl) ◽

pp. 2-23 ◽

Cited By ~ 7

Author(s):

Goro Mogi

Keyword(s):

Immunoglobulin A ◽

Secretory Component ◽

Secretory Iga ◽

Secretory Cells ◽

Antigenic Relationship ◽

Serum Iga ◽

Human Colostrum ◽

Specific Antisera ◽

Antigenic Relationships ◽

Secretory Immunoglobulin

Secretory IgA (SIgA) is the predominant immunoglobulin in certain external secretions and may have an important role in immunological mucosal resistance. SIgA differs in chemical and immunological properties from serum IgA. The present study was undertaken to investigate the antigenic relationship between SIgA, free secretory component (FSC) and serum IgA and the localization of SIgA as well as other immunological classes in tissues of oral and respiratory passages by use of immunofluorescence technique. SIgA and FSC were highly purified from human colostrum and rabbit anti-SIgA and anti-SC antisera were prepared. On the basis of antigenic relationships between SIgA, FSC and serum IgA, it was emphasized that individual specific antisera for SC and IgA and/or SIgA should be used in immunochemical or immunohistological investigations for SIgA. The present study failed to detect SC determinants in palatine and lingual tonsils. However, it was evident that cells present in the pharyngeal tonsillar epithelium contain SC determinants. SC molecules may be synthesized in certain secretory cells of mucous membrane and glandular epithelium and the combining of SC with IgA could occur in the cytoplasm of epithelial cells, the intercellular spaces and/or in the lumens of glandular acini and ductules.

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