Amino acid tolerance in cirrhotic patients following oral protein and amino acid loads

Genes encoding the subunits of the membrane-bound F1F0-ATPase (responsible for exporting protons from the cytoplasm and contributing to acid tolerance) were sequenced for 24 non-mutans streptococci isolated from carious lesions. Isolates, mostly Streptococcus salivarius, displayed a continuum of acid tolerance thresholds ranging from pH 4.55 to 3.39, but amino acid alignments of F1F0-ATPase subunits revealed few non-synonymous substitutions and these were unrelated to acid tolerance. Thus, the F1F0-ATPase is highly-conserved among S. salivarius isolates despite varying acid tolerance thresholds, supporting the contention that acid tolerance is determined by the level of gene/protein expression rather than variation in molecular structure.

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Is administrating branched-chain amino acid-enriched nutrition achieved symptom-free in malnourished cirrhotic patients?

Journal of Clinical Biochemistry and Nutrition ◽

10.3164/jcbn.13-64 ◽

2014 ◽

Vol 54 (1) ◽

pp. 51-54 ◽

Cited By ~ 5

Author(s):

Yasuhiro Tsuda ◽

Hideo Fukui ◽

Tetsuya Sujishi ◽

Hideko Ohama ◽

Yusuke Tsuchimoto ◽

...

Keyword(s):

Amino Acid ◽

Branched Chain Amino Acid ◽

Cirrhotic Patients ◽

Branched Chain

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Oral branched-chain amino acid granules improve structure and function of human serum albumin in cirrhotic patients

Journal of Gastroenterology ◽

10.1007/s00535-016-1281-2 ◽

2016 ◽

Vol 52 (6) ◽

pp. 754-765 ◽

Cited By ~ 14

Author(s):

Hiroko Setoyama ◽

Motohiko Tanaka ◽

Kohei Nagumo ◽

Hideaki Naoe ◽

Takehisa Watanabe ◽

...

Keyword(s):

Amino Acid ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Structure And Function ◽

Branched Chain Amino Acid ◽

Cirrhotic Patients ◽

Branched Chain ◽

And Function

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Reduction of the Myofibrillar Protein Breakdown Rate in Cirrhotic Patients by Long-Term Oral Administration of a Branched-Chain Amino Acid Mixture

Advances in Hepatic Encephalopathy and Urea Cycle Diseases ◽

10.1159/000410871 ◽

2015 ◽

pp. 700-705

Author(s):

F. S. Dioguardi ◽

R. Abbiati ◽

P. Incerti ◽

M. Brigatti ◽

M. Dell�Oca

Keyword(s):

Amino Acid ◽

Oral Administration ◽

Amino Acid Mixture ◽

Myofibrillar Protein ◽

Acid Mixture ◽

Breakdown Rate ◽

Branched Chain Amino Acid ◽

Cirrhotic Patients ◽

Branched Chain

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Identification and Inactivation of Genetic Loci Involved with Lactobacillus acidophilus Acid Tolerance

Applied and Environmental Microbiology ◽

10.1128/aem.70.9.5315-5322.2004 ◽

2004 ◽

Vol 70 (9) ◽

pp. 5315-5322 ◽

Cited By ~ 97

Author(s):

M. Andrea Azcarate-Peril ◽

Eric Altermann ◽

Rebecca L. Hoover-Fitzula ◽

Raul J. Cano ◽

Todd R. Klaenhammer

Keyword(s):

Amino Acid ◽

Lactobacillus Acidophilus ◽

Acid Stress ◽

Acid Tolerance ◽

Low Ph ◽

Open Reading Frames ◽

Amino Acid Permease ◽

Physiological Limits ◽

Insertional Inactivation ◽

Genes Encoding

ABSTRACT Amino acid decarboxylation-antiporter reactions are one of the most important systems for maintaining intracellular pH between physiological limits under acid stress. We analyzed the Lactobacillus acidophilus NCFM complete genome sequence and selected four open reading frames with similarities to genes involved with decarboxylation reactions involved in acid tolerance in several microorganisms. Putative genes encoding an ornithine decarboxylase, an amino acid permease, a glutamate γ-aminobutyrate antiporter, and a transcriptional regulator were disrupted by insertional inactivation. The ability of L. acidophilus to survive low-pH conditions, such as those encountered in the stomach or fermented dairy foods, was investigated and compared to the abilities of early- and late-stationary-phase cells of the mutants by challenging them with a variety of acidic conditions. All of the integrants were more sensitive to low pH than the parental strain. Interestingly, each integrant also exhibited an adaptive acid response during logarithmic growth, indicating that multiple mechanisms are present and orchestrated in L. acidophilus in response to acid challenge.

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