Panagrellus redivivus mass produced on solid media as live food for Litopenaeus vannamei larvae

2006 ◽  
Vol 37 (14) ◽  
pp. 1429-1436 ◽  
Author(s):  
Ulfert Focken ◽  
Christian Schlechtriem ◽  
Matthias von Wuthenau ◽  
Armando García-Ortega ◽  
Ana Puello-Cruz ◽  
...  
Author(s):  
Nabil Majdi ◽  
Sebastian Weber ◽  
Walter Traunspurger

In this study, we measured the daily consumption of four different nematode species by a small freshwater catfish species, Corydoras aeneus (Gill, 1858). Consumption of nematodes by fishes was significant with a single C. aeneus individual being able to consume in 24 h between 40 581 and 75 849 adult nematodes depending on the nematode species offered. This represented the ingestion of up to 238 mg wet weight when considering the largest nematode species: Panagrellus redivivus. Our results strengthen the growing evidence of a significant trophic channel existing between meiobenthic invertebrates like nematodes and small bottom-feeding fishes like C. aeneus. We also discuss the relevance of using P. redivivus as live food for rearing C. aeneus which is a popular ornamental fish.


2020 ◽  
Vol 8 (2) ◽  
pp. 111-122
Author(s):  
Diana Chilmawati ◽  
Suminto Suminto

The development of vaname shrimp (Litopenaeus vannamei) culture will affect the increasing need of live food organisms for shrimp larvae, especially Artemia sp. In shrimp hatchery, biotechnical constraints of culture of Artemia sp. and dependence on imported cysts is a limiting factor for success. Perhaps, the use of Oithona sp. is an alternative live food for L. vannamei larvae which has the potential to be combined with Artemia sp. The aim of this study was to examine the effect of a combination of live foods, Artemia sp. and Oithona sp. on the survival rate of L. vannamei shrimp larvae and to determine the percentage of live food combinations that provide the best survival rate of L. vannamei shrimp larvae. The method used was an experiment carried out at the Coastal Area Development Laboratory, Jepara. The experimental design used CRD with 5 treatments and 4 replications. Those treatments were a combination of live food, Artemia sp. and Oithona sp. with the percentage ratio of each treatment A. 100%: 0%; B. 75%: 25%; C. 50%: 50%; D. 25%: 75%; and E. 0%: 100%. The results showed that the combination of live foods of Artemia sp. and Oithona sp. had a significant effect (p<.05) on the survival rate of the L. vannamei  shrimp larvae and the percentage of the combination of natural foods, Artemia sp. ranged of 50 -100% and Oithona sp ranged of 0% - 50% had given the survival rates of L. vannamei larvae (N-5/6 to PL-10)  ranged of 41.71-43.00%.


2019 ◽  
Author(s):  
Carlos Andrés Silva-Aldas ◽  
Annette Milena Villalta-Gordillo ◽  
Jocelyn Estefanía Arias-Naranjo ◽  
Ricardo Burgos-Morán

2004 ◽  
Vol 35 (6) ◽  
pp. 547-551 ◽  
Author(s):  
Christian Schlechtriem ◽  
Manuele Ricci ◽  
Ulfert Focken ◽  
Klaus Becker

2007 ◽  
Vol 38 (6) ◽  
pp. 653-659 ◽  
Author(s):  
Jürgen Sautter ◽  
Horst Kaiser ◽  
Ulfert Focken ◽  
Klaus Becker

Author(s):  
Karen S. Howard ◽  
H. D. Braymer ◽  
M. D. Socolofsky ◽  
S. A. Milligan

The recently isolated cell wall mutant slime X of Neurospora crassa was prepared for ultrastructural and morphological comparison with the cell wall mutant slime. The purpose of this article is to discuss the methods of preparation for TEM and SEM observations, as well as to make a preliminary comparison of the two mutants.TEM: Cells of the slime mutant were prepared for thin sectioning by the method of Bigger, et al. Slime X cells were prepared in the same manner with the following two exceptions: the cells were embedded in 3% agar prior to fixation and the buffered solutions contained 5% sucrose throughout the procedure.SEM: Two methods were used to prepare mutant and wild type Neurospora for the SEM. First, single colonies of mutant cells and small areas of wild type hyphae were cut from solid media and fixed with OSO4 vapors similar to the procedure used by Harris, et al. with one alteration. The cell-containing agar blocks were dehydrated by immersion in 2,2-dimethoxypropane (DMP).


Author(s):  
Jane Payne ◽  
Philip Coudron

This transmission electron microscopy (TEM) procedure was designed to examine a gram positive spore-forming bacillus in colony on various solid agar media with minimal artifact. Cellular morphology and organization of colonies embedded in Poly/Bed 812 resin (P/B) were studied. It is a modification of procedures used for undecalcified rat bone and Stomatococcus mucilaginosus.Cultures were fixed and processed at room temperature (RT) under a fume hood. Solutions were added with a Pasteur pipet and removed by gentle vacuum aspiration. Other equipment used is shown in Figure 3. Cultures were fixed for 17-18 h in 10-20 ml of RT 2% phosphate buffered glutaraldehyde (422 mosm/KgH2O) within 5 m after removal from the incubator. After 3 (30 m) changes in 0.15 M phosphate buffer (PB = 209-213 mosm/KgH2O, pH 7.39-7.41), colony cut-outs (CCO) were made with a scalpel.


Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
A Azzollini ◽  
JL Wolfender ◽  
K Gindro

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