scholarly journals The effect of gold sodium thiomalate and auranofin on lipopolysaccharide-induced interleukin-1 production by blood monocytes in vitro: variation in healthy subjects and patients with arthritis

2008 ◽  
Vol 79 (3) ◽  
pp. 335-340 ◽  
Author(s):  
V. A. DANIS ◽  
A. J. KULESZ ◽  
D. S. NELSON ◽  
P. M. BROOKS
Keyword(s):  
Healthy Subjects ◽  
Interleukin 1 ◽  
Gold Sodium Thiomalate ◽  
Blood Monocytes ◽  
Gold Sodium

Is the Success of Cefazolin plus Ertapenem in Methicillin-Susceptible Staphylococcus aureus Bacteremia Based on Release of Interleukin 1-beta?

2022 ◽  
Author(s):  
Dan Smelter ◽  
Mary Hayney ◽  
George Sakoulas ◽  
Warren Rose
Keyword(s):  
Staphylococcus Aureus ◽  
Peripheral Blood ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Blood Monocytes ◽  
Salvage Regimen ◽  
Peripheral Blood Monocytes ◽  
Staphylococcus Aureus Bacteremia

Cefazolin and ertapenem has been shown to be an effective salvage regimen for refractory methicillin-susceptible Staphylococcus aureus bacteremia. Our findings suggest cefazolin plus ertapenem in vitro stimulates interleukin-1β release from peripheral blood monocytes both with and without S. aureus presence. This IL-1β augmentation was primarily driven by ertapenem. These findings support further exploration of cefazolin plus ertapenem in MSSA bacteremia and may partially explain its marked potency in vivo despite modest synergy in vitro .

scholarly journals Release of interleukin-1 and interleukin-6 from human monocytes by antithymocyte globulin: requirement for de novo synthesis

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2531-2538 ◽  
Author(s):  
P Rameshwar ◽  
P Gascon
Keyword(s):  
Antithymocyte Globulin ◽  
De Novo ◽  
Interleukin 1 ◽  
Immunosuppressive Agent ◽  
Biologically Active ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
De Novo Synthesis ◽  
Hematopoietic Growth Factors

Abstract Antithymocyte globulin (ATG) is an effective treatment in patients with severe aplastic anemia (SAA). Its mechanism of action remains unclear, although it has been assumed to be immunosuppressive. However, ATG has also been shown by several laboratories to be immunostimulatory. Recently, interleukin-1 (IL-1) production has been found to be decreased in lipopolysaccharide-stimulated peripheral blood monocytes obtained from SAA patients. We have investigated the ability of ATG to function as an immunostimulatory agent via the production of IL-1 and IL-6 by normal human monocytes in vitro. Supernatants from ATG- stimulated monocytes were assayed for biologically active and immunoreactive IL-1 and IL-6. We have found that ATG, via its F(ab')2 fragment is a powerful inducer of IL-1 and IL-6 production. Furthermore, ATG induction of both cytokines from normal monocytes required de novo synthesis, as determined by 35S-methionine incorporation. Because these two cytokines synergize with other cytokines at both the stem cell and progenitor levels, these stimulatory properties of ATG may be relevant to the treatment of SAA. This would favor the hypothesis of a bimodal mechanism for ATG as an inducer of hematopoietic growth factors and as an immunosuppressive agent.

scholarly journals Stimulation of Human Monocytes with the Gram-Positive Vaccine Vector Streptococcus gordonii

2006 ◽  
Vol 13 (9) ◽  
pp. 1037-1043 ◽  
Author(s):  
Annalisa Ciabattini ◽  
Anna Maria Cuppone ◽  
Rita Pulimeno ◽  
Francesco Iannelli ◽  
Gianni Pozzi ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Dendritic Cell Maturation ◽  
Vaccine Vector ◽  
Human Monocytes ◽  
Streptococcus Gordonii ◽  
Blood Monocytes ◽  
Immunostimulatory Activity ◽  
Necrosis Factor Alpha

ABSTRACT Streptococcus gordonii is a bacterial vaccine vector which has previously been shown to activate dendritic cells in vitro and to induce local and systemic immune responses in vivo. In the present study, human monocytes (THP-1 cell line and peripheral blood monocytes) were characterized following interaction with S. gordonii. Treatment of human monocytes with S. gordonii but not latex beads induced a clear up-regulation of CD83, CD40, CD80, and CD54 and the down-regulation of CD14. Furthermore, bacterial treatment stimulated an increased expression of Toll-like receptor 5 (TLR5), TLR6, and TLR7, production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin 1 beta, and reduction of the phagocytic activity. This work shows that the immunostimulatory activity of S. gordonii is not restricted to induction of dendritic-cell maturation but also affects the differentiation process of human monocytes.

scholarly journals A Cytofluorometric Study of Membrane Rafts in Human Monocyte Subsets in Atherosclerosis

Acta Naturae ◽  
2014 ◽  
Vol 6 (4) ◽  
pp. 80-88 ◽  
Author(s):  
M. A. Chelombitko ◽  
V. S. Shishkina ◽  
O. P. Ilyinskaya ◽  
A. I. Kaminnyi ◽  
T. O. Pavlunina ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Human Monocyte ◽  
Membrane Rafts ◽  
Main Function ◽  
Membrane Raft ◽  
Blood Monocytes ◽  
In Vitro Differentiation ◽  
Monocyte Subsets ◽  
Activated State

The peripheral blood monocytes of atherosclerotic patients are pre-activated and have some of the features of tissue macrophages. Their adhesion to the endothelium is 1.5 times higher than that of monocytes from healthy subjects, and they express a number of receptors and antigens typical of tissue macrophages. Additionally, earlier we showed that the biosynthesis of gangliosides, whose main function is the formation of membrane rafts, is significantly activated in blood monocytes from atherosclerotic patients, as well as during the in vitro differentiation of normal monocytes into macrophages. In this study, we investigated the expression of membrane rafts on various monocyte subsets from healthy subjects and atherosclerotic patients. Based on flow cytometry results, the monocytes in the examined atherosclerotic patients were found to differ from those in healthy subjects by a twofold increase in the proportion of the intermediate subset (CD14 ++/CD16 +) and by enhancement in the expression of the fractalkine receptor CX3CR1 on the intermediate and non-classical subsets (CD14 ++/CD16 + and CD14 +/CD16 ++) (2.3 and 1.8 times, respectively). This suggests a pre-activated state of monocytes in atherosclerotic patients. At the same time, the expression of the membrane raft marker on the monocyte subsets was similar in both studied groups. However, a study of the in vitro differentiation of monocytes into macrophages showed that the membrane raft expression increased 2 times as early as on the 1st day of culturing and 3 times on the 7th day compared to that in freshly isolated monocytes. Therefore, it is suggested that monocytes in atherosclerosis accumulate gangliosides that are used to form membrane rafts during the macrophage differentiation after the migration of monocytes into the arterial intima.

Peritoneal Sclerosis: Role of Plasticizers in Stimulating Interleukin-1 Production

1993 ◽  
Vol 13 (2_suppl) ◽  
pp. 517-519 ◽  
Author(s):  
Agostino Fracasso ◽  
Lorenzo Calo ◽  
Silvano Landini ◽  
Paolo Morachiello ◽  
Flavio Righetto ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Interleukin 1 ◽  
Final Concentration ◽  
The Other ◽  
Fibroblast Proliferation ◽  
Two Cultures

The role of plasticizers (PLS) in inducing water flow inhibition and peritoneal sclerosis has been demonstrated in both In vivo and In vitro studies. Interleukin-1 (IL-1) has been shown to be a regulator of fibroblast proliferation as well as collagenase production. The aim of this study was to evaluate the role of PLS in stimulating mononuclear ce1l1L-1 secretion. Two cultures containing 103 cells/mL were obtained from 14 healthy subjects. One was used as the control, and the other was mixed with diethylhexylphthalate (DEHP) to reach a final concentration of 2.8x10” M. After 4 hours the samples were centrifuged, and the supernatants were tested by radioimmunoassay for IL-1α and IL-1β. The results showed a significant increase In both IL-1α and IL-1 β production In DEHP-stimulated cells in comparIson to the controls: 42.6±15.4 versus 29.3±10 ng/L (p<0.015) for IL-1α. and 153.6±55 versus 113.6±32 ng/L (p<0.03) for IL-1β In conclusion, PLS added to mononuclear cells were able to Induce IL-1 secretion. This mechanism could be responsible, at least in part, for the development of peritoneal sclerosis. Thus the employment of plasticlzer-free bags should be elective in peritoneal dialysis.

Distribution of gold in human platelets after in-vitro interaction and during chrysotherapy with gold sodium thiomalate

1992 ◽  
Vol 1 (4) ◽  
pp. 295-303 ◽  
Author(s):  
Y. B. Kassam ◽  
W. F. Kean ◽  
C. J. L. Lock ◽  
G. T. Simon ◽  
W. W. Buchanan
Keyword(s):  
Human Platelets ◽  
Gold Sodium Thiomalate ◽  
Gold Sodium ◽  
In Vitro Interaction

scholarly journals Release of interleukin-1 and interleukin-6 from human monocytes by antithymocyte globulin: requirement for de novo synthesis

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2531-2538
Author(s):  
P Rameshwar ◽  
P Gascon
Keyword(s):  
Antithymocyte Globulin ◽  
De Novo ◽  
Interleukin 1 ◽  
Immunosuppressive Agent ◽  
Biologically Active ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
De Novo Synthesis ◽  
Hematopoietic Growth Factors

Antithymocyte globulin (ATG) is an effective treatment in patients with severe aplastic anemia (SAA). Its mechanism of action remains unclear, although it has been assumed to be immunosuppressive. However, ATG has also been shown by several laboratories to be immunostimulatory. Recently, interleukin-1 (IL-1) production has been found to be decreased in lipopolysaccharide-stimulated peripheral blood monocytes obtained from SAA patients. We have investigated the ability of ATG to function as an immunostimulatory agent via the production of IL-1 and IL-6 by normal human monocytes in vitro. Supernatants from ATG- stimulated monocytes were assayed for biologically active and immunoreactive IL-1 and IL-6. We have found that ATG, via its F(ab')2 fragment is a powerful inducer of IL-1 and IL-6 production. Furthermore, ATG induction of both cytokines from normal monocytes required de novo synthesis, as determined by 35S-methionine incorporation. Because these two cytokines synergize with other cytokines at both the stem cell and progenitor levels, these stimulatory properties of ATG may be relevant to the treatment of SAA. This would favor the hypothesis of a bimodal mechanism for ATG as an inducer of hematopoietic growth factors and as an immunosuppressive agent.

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