Changes in the surface of four calcium silicate-containing endodontic materials and an epoxy resin-based sealer after a solubility test

The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.

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Efficacy and retrievability of root canal filling using calcium silicate‐based and epoxy resin‐based root canal sealers with matched obturation techniques

Australian Endodontic Journal ◽

10.1111/aej.12323 ◽

2019 ◽

Vol 45 (3) ◽

pp. 337-345 ◽

Cited By ~ 2

Author(s):

Seo Ryeong Kim ◽

Sang Won Kwak ◽

Ju‐Kyung Lee ◽

Hye‐Jin Goo ◽

Jung‐Hong Ha ◽

...

Keyword(s):

Epoxy Resin ◽

Root Canal ◽

Calcium Silicate ◽

Root Canal Filling ◽

Root Canal Sealers

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A Comparative Chemical Study of Calcium Silicate-Containing and Epoxy Resin-Based Root Canal Sealers

BioMed Research International ◽

10.1155/2016/9808432 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Przemysław Reszka ◽

Alicja Nowicka ◽

Mariusz Lipski ◽

Włodzimierz Dura ◽

Agnieszka Droździk ◽

...

Keyword(s):

Epoxy Resin ◽

Root Canal ◽

Calcium Silicate ◽

Chemical Elements ◽

Petri Dish ◽

Chemical Study ◽

X Ray ◽

Sodium Magnesium ◽

Inner Diameter ◽

Root Canal Sealers

Objective.The present study assessed the chemical elements in two novel calcium silicate-containing root canal sealers, BioRoot RCS and Well-Root ST, compared to a calcium silicate-containing root canal sealer that has been on the market for several years, MTA Fillapex, and epoxy resin-based sealer AHPlus.Material and Methods.The sealers were mixed and manipulated according to the manufacturers’ instructions. Twelve cylindrical molds (inner diameter 4 mm; height 3 mm) were placed on a glass petri dish and packed with the materials. The dish was transferred to an incubator. After 72 h the molds were examined by scanning electron microscopy and energy dispersive X-ray microanalysis.Results.BioRoot RCS and Well-Root ST had high peaks of calcium, zirconium, oxygen, carbon, silicon, and chlorine. Well-Root ST also had sodium, magnesium, aluminum, and titanium peaks. MTA Fillapex and AHPlus had carbon, oxygen, calcium, titanium, and bismuth peaks. A silicon peak was also observed for MTA Fillapex, and zirconium and tungsten peaks for AHPlus.Conclusion.BioRoot RSC had the highest degree of purity. The clinical implication of metals contained in the other sealers needs to be investigated.

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