Relative Role of Individual Phospholipids on Thiobarbituric Acid Reactive Substances Formation in Chicken Meat, Skin and Swine Aorta

1990 ◽  
Vol 55 (5) ◽  
pp. 1243-1248 ◽  
Author(s):  
JAN PIKUL ◽  
FRED A. KUMMEROW
Keyword(s):  
Thiobarbituric Acid ◽  
Chicken Meat ◽  
Relative Role ◽  
Thiobarbituric Acid Reactive Substances

scholarly journals Lens Lipid Peroxides and Glutathione Concentrations in Diabetic Cataract

1997 ◽  
Vol 34 (2) ◽  
pp. 190-192 ◽  
Author(s):  
D Özmen ◽  
I Mutaf ◽  
B Özmen ◽  
J Mentes ◽  
O Bayindir
Keyword(s):  
G Protein ◽  
Lipid Peroxide ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Diabetic Cataract ◽  
Free Radical Damage ◽  
Significant Difference ◽  
Radical Damage

This study aims to explore the role of reactive oxygen radicals in the genesis of diabetic cataract. Lipid peroxide (LPO) concentrations in senile ( n = 30) and diabetic ( n = 14) cataractous lenses, were determined as thiobarbituric acid-reactive substances (TBARS) by a method modified from Satoh and Yagi, and reduced glutathione (GSH) concentrations were measured according to Beutler. Lens LPO levels (mean, SD; nmol TBARS/g protein) were significantly higher in diabetics (107·54, 18·12) than senile cataractous subjects (53·54, 15·48) ( P < 0·0001). Lens GSH levels (mean, SD; nmol/g protein) showed no significant difference between diabetics (4·29, 2·05) and senile cataractous subjects (4·68, 3·12). These results suggest that free radical damage is more effective in the genesis of diabetic cataract than in senile cataract.

Reheating Effect on Thiobarbituric Acid Reactive Substances of Refrigerated Stored, Cooked Broiler Meat

1992 ◽  
Vol 55 (11) ◽  
pp. 924-926 ◽  
Author(s):  
C. Y. W. ANG
Keyword(s):  
Storage Time ◽  
Thiobarbituric Acid ◽  
Chicken Meat ◽  
Internal Temperature ◽  
Thiobarbituric Acid Reactive Substances ◽  
Muscle Type ◽  
Broiler Meat ◽  
Broiler Breast ◽  
Negligible Contribution ◽  
Precooked Chicken Meat

Broiler breast and leg meats in sealed bags were cooked in an 88°C water bath to an internal temperature of 81 °C. Product was cooled and stored at 4°C for 0 or 3 d. Samples were reheated to 60°C in a 163°C oven. No significant differences were found by the thiobarbituric acid reactive substances test between control and reheated portions, regardless of muscle type or storage time after cooking. The reheating practice made negligible contribution to oxidative changes of precooked chicken meat.

Membrane-type matrix metalloproteinase-9 activity in placental tissue from patients with pre-existing and gestational diabetes mellitus

2000 ◽  
Vol 12 (6) ◽  
pp. 269 ◽  
Author(s):  
Carolina Pustovrh ◽  
Alicia Jawerbaum ◽  
Debora Sinner ◽  
Mario Pesaresi ◽  
Mario Baier ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Matrix Metalloproteinase ◽  
Placental Tissue ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Sod Activity ◽  
No Donors ◽  
Membrane Type ◽  
Metalloproteinase 9

The activity of matrix metalloproteinase (MMP)-9 was evaluated in placental tissue from healthy subjects (controls) and from patients with gestational and pre-existing diabetes mellitus (GDM and PDM, respectively). Compared with controls, MMP-9 activity was greater in placental tissue from patients with PDM and lower in placental tissue from patients with GDM. The modulatory role of nitric oxide (NO) and reactive oxygen species (ROS) on MMP-9 activity in placental tissue was evaluated. In healthy placenta, NO synthase inhibitors diminished MMP-9 activity, whereas NO donors enhanced it. The addition of xanthine/xanthine oxidase or hydrogen peroxide to placental incubates enhanced MMP-9 activity, while the addition of superoxide dismutase (SOD) diminished it. In placental tissue from patients with PDM, MMP-9 activity was stimulated by NO and by ROS. In placental tissue from patients with PDM, concentrations of nitrates/nitrites and thiobarbituric acid-reactive substances (TBARS) were enhanced, whereas SOD activity was decreased, suggesting that elevated concentrations of NO and ROS may be related to the enhanced MMP-9 concentrations found in these tissues. In placenta from GDM patients, in which a diminished concentration of MMP-9 were detected, nitrate/nitrite concentrations were increased, but placental MMP-9 activity did not change in the presence of either NO donors or inhibitors. The activity of MMP-9 in placental tissue from patients with GDM was stimulated by ROS donor systems and was inhibited by the addition of SOD; however, TBARS and SOD concentrations were unchanged in these tissues compared with controls. These findings demonstrate that placental MMP-9 activity is modulated by NO and ROS and that, in diabetic pathology, NO and ROS may determine changes in MMP-9 activity, which are probably involved in the structural and functional abnormalities of diabetic placental tissue.

scholarly journals Influence of modified atmosphere packaging on freshness parameters of organic chicken meat – short communication

2017 ◽  
Vol 35 (No. 5) ◽  
pp. 466-468 ◽  
Author(s):  
Abdullah Fouad Ali Abdullah ◽  
Buchtová Hana ◽  
Turek Peter
Keyword(s):  
Antioxidant Capacity ◽  
Short Communication ◽  
Modified Atmosphere Packaging ◽  
Thiobarbituric Acid ◽  
Chicken Meat ◽  
Modified Atmosphere ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Samples

The effect of modified atmosphere packaging (MAP1 80% O2/20% CO2 and MAP2 70% N2/30% CO2) on the selected parameters (ammonia, thiobarbituric acid reactive substances /TBARS/) and antioxidant capacity in chilled meat (2 ± 2°C) of organic chickens (breast and thigh) was evaluated. Control samples were packaged using a polyolefin film. The experiment was conducted on day 2 and repeated on day 7, 10, and 14 of storage. TBARS of samples in MAP1 were higher than in samples stored in MAP2. Ammonia in meat in MAP2 was constant during storage. In general, the DPPH inhibition percentage of meat in MAP1 was lower than that in meat in MAP2. Results of the present study indicated that MAP2 could be preferable for the packaging of organic chicken meat.

Role of antioxidant defenses during estivation and anoxia exposure in the freshwater snail Biomphalaria tenagophila (Orbigny, 1835)

2003 ◽  
Vol 81 (7) ◽  
pp. 1239-1248 ◽  
Author(s):  
Marcus V.R Ferreira ◽  
Antonieta C.R Alencastro ◽  
Marcelo Hermes-Lima
Keyword(s):  
Thiobarbituric Acid ◽  
Freshwater Snail ◽  
Antioxidant Defenses ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Gpx Activity

The effects of 24 h of exposure to underwater anoxia and 15 days of estivation (at 26–27°C) on the enzymatic antioxidant system of the hepatopancreas of the freshwater snail Biomphalaria tenagophila (Planorbidae) are described. The effect of 24 h of recovery was also investigated. Catalase activity dropped by 31% during 24 h of anoxia, and superoxide dismutase (SOD) activity was reduced by 43% during the 15 days of estivation. This is consistent with the overall decrease in metabolic rate during estivation or anoxia. Indeed, the heartbeat diminished by 28–36% during estivation (determination was possible for only 4 days) and by 66% after 24 h of anoxia. On the other hand, selenium-dependent glutathione peroxidase (Se-GPX) activity increased during anoxia (from 10 to 14 mU/mg protein) and estivation (by 14%). Glutathione S-transferase (GST) and glutathione reductase activities remained unchanged during estivation and anoxia. Glucose 6-phosphate dehydrogenase activity was unchanged during estivation and recovery. Recovery restored SOD activity. Catalase, Se-GPX, and GST activities during recovery were significantly lower than those of the respective controls. Lipid peroxidation, determined as the level of thiobarbituric acid-reactive substances, was unchanged in the hepatopancreas after 15 days of estivation and 26 h of recovery from estivation. It is possible that the increase in Se-GPX activity during anoxia and estivation, and the maintenance of GST activity, are relevant in minimizing the effects of reactive oxygen species that can be formed upon resumption of aerobic metabolism. Thus, B. tenagophila may have a biochemical strategy of preparation for oxidative stress such as that observed in several other species of anoxia/hypoxia-tolerant animals.

scholarly journals Abscisic Acid in Coordination with Nitrogen Alleviates Salinity-Inhibited Photosynthetic Potential in Mustard by Improving Proline Accumulation and Antioxidant Activity

Stresses ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 162-180
Author(s):  
Arif Majid ◽  
Bilal A. Rather ◽  
Asim Masood ◽  
Zebus Sehar ◽  
Naser A. Anjum ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Abscisic Acid ◽  
Photosynthetic Activity ◽  
Thiobarbituric Acid ◽  
Salinity Treatment ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Photosynthetic Potential

This investigation was done to assess the role of abscisic acid (ABA; 25 µM) and/or nitrogen (N; 10 mM) in the alleviation of salinity (NaCl; 100 mM)-induced reduction in photosynthetic activity and growth, N and sulfur (S) assimilation of mustard (Brassica juncea L.) cv. RH0-749. Salinity treatment caused oxidative stress and significantly elevated the content of both H2O2 and thiobarbituric acid reactive substances (TBARS), and impaired photosynthetic activity and growth, but increased the content of nitrogenous osmolyte proline and the activity of antioxidant enzymes involved in the metabolism of reactive oxygen species. The application of 25 µM ABA under a controlled condition negatively affected photosynthesis and growth. However, ABA, when combined with N, minimized oxidative stress and mitigated the salinity-inhibited effects by increasing the activity of antioxidant enzymes (superoxide dismutase, SOD; glutathione reductase, GR; ascorbate peroxidase, APX) and proline content. Overall, the supplementation of 10 mM N combined with 25 µM ABA provides an important strategy for enhancing the photosynthetic potential of B. juncea under saline conditions.

Anti-ulcer role of herbomineral siddha drug — Thamira parpam on experimentally induced gastric mucosal damage in rats

2010 ◽  
Vol 29 (3) ◽  
pp. 161-173 ◽  
Author(s):  
M. Vasanthkumar ◽  
RP Parameswari ◽  
V. Vijaya Kumar ◽  
MK Sangeetha ◽  
V. Gayathri ◽  
...  
Keyword(s):  
Thiobarbituric Acid ◽  
Mucosal Damage ◽  
Gastric Volume ◽  
Gastric Mucosal Damage ◽  
Thiobarbituric Acid Reactive Substances ◽  
Pylorus Ligation ◽  
Ulcerogenic Activity ◽  
Per Oral ◽  
Experimentally Induced

Anti-ulcerogenic activity of Thamira parpam (TP) was investigated in two ulcer models (aspirin + pylorus ligation and HCl-ethanol). Aspirin—pylorus ligation (Asp 200 mg/kg-7 days + PL-4 hours) and HCl-ethanol (150 mM HCl in 70% ethanol) induction in rat resulted in elevation of thiobarbituric acid reactive substances (TBARS) and depletion of antioxidants (superoxide dismutase [SOD], glutathione [GSH], glutathione peroxidase [GPx]) with high ulcer scores (p < .01). In Asp + PL model, TP treatment showed mild inhibition on ulcer scores, changes in pH, gastric volume, total and free acidity, and elevation of TBARS and depletion of antioxidants. Compared to the ulcer-untreated rats (HCl-ethanol), the herbomineral drug TP treatment (0.5, 1, 2 mg/kg, per oral [p.o.]) attenuated the elevation of TBARS, decrease of antioxidants and nitrite (p < .05). Histopathological examinations were correlated with the antioxidant profile. In conclusion, the prophylactic cytoprotective nature of the herbomineral drug in experimentally induced ulcers could be mediated by its free radical quenching property.

Dexmedetomidine protects blood δ-aminolevulinate dehydratase from inactivation caused by hyperoxygenation in total intravenous anesthesia

2010 ◽  
Vol 30 (4) ◽  
pp. 289-295 ◽  
Author(s):  
João BT Rocha ◽  
Neusa M Heinzmann Bulow ◽  
Eduardo FM Correa ◽  
Cassiano Scholze ◽  
Cristina W Nogueira ◽  
...  
Keyword(s):  
Continuous Infusion ◽  
Molecular Oxygen ◽  
Human Blood ◽  
Thiobarbituric Acid ◽  
Total Intravenous Anesthesia ◽  
Thiobarbituric Acid Reactive Substances ◽  
Intravenous Anesthesia ◽  
Aminolevulinate Dehydratase ◽  
Remifentanil Group

Delta-aminolevulinate dehydratase (δ-ALA-D) enzyme is sensitive to pro-oxidant agents, including molecular oxygen. Here, we tested whether hyperoxygenation after total intravenous (i.v.) anesthesia could interact with the type of anesthesia (dexmedetomidine, continuous infusion; 0.5 μg/kg/h or remifentanil, continuous infusion; 0.3 μg/kg/min) plus propofol using blood δ-ALA-D activity and thiobarbituric acid reactive substances (TBARS) levels as ending points of toxicity. In absence or presence of dithiothreitol (DTT), δ-ALA-D activity was reduced after hyperoxygenation in the group treated with remifentanil and was not modified in dexmedetomidine group. TBARS increased considerably in the blood of both groups of patients after oxygenation. The results obtained here suggest that the hyperoxygenation was associated with a marked increase in TBARS production regardless of the type of anesthesia. δ-ALA-D activity was only inhibited in remifentanil group, which indicates a possible interaction between oxygenation and the type of anesthetic. This is the first demonstration that dexmedetomidine may protect blood δ-ALA-D from oxidation. However, further studies are necessary to establish a possible antioxidant role of dexmedetomidine against hyperoxygenation in human blood.

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