Accuracy of telomerase in cervical lesions: a systematic review

2007 ◽  
Vol 17 (6) ◽  
pp. 1205-1214 ◽  
Author(s):  
M. I. Rosa ◽  
L. R. Medeiros ◽  
M. C. Bozzetti ◽  
J. Fachel ◽  
E. Wendland ◽  
...  
Keyword(s):  
Systematic Review ◽  
Intraepithelial Neoplasia ◽  
Cervix Cancer ◽  
Early Event ◽  
Low Grade ◽  
Cervical Carcinogenesis ◽  
Benign Lesions ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
Intraepithelial Lesions

The detection of telomerase activity in cervix may provide information on cervical carcinogenesis and may be a marker to monitor cervical intraepithelial neoplasia transition. A quantitative systematic review was performed to estimate the accuracy of telomerase assay in cervical lesions. Studies that evaluated the telomerase test (telomerase repeated amplification protocol) for the diagnosis of cervix lesions and compared it to paraffin-embedded sections as the diagnostic standard were included. Ten studies were analyzed, which included 1069 women. The diagnostic odds ratio (DOR) for a positive telomerase test for low-grade squamous intraepithelial lesions (Lo-SIL) vs normal or benign lesions was 3.2 (95% CI, 1.9–5.6). The DOR for a positive telomerase test for high-grade squamous intraepithelial lesions (Hi-SIL) vs Lo-SIL, normal or benign lesions was 5.8 (95% CI, 3.1–10). For cervix cancer vs Hi-SIL, the DOR for a positive telomerase test was 8.1 (95% CI, 3.2–20.3) and for cervix cancer vs Lo-SIL, normal or benign lesions, it was 40.9 (95% CI, 18.2–91). Our data support the current hypothesis that telomerase may activate an early event in cervical carcinogenesis that could be associated with the initiation and progression of cervical lesions

scholarly journals HPV Testing and Pap Test: Role for a Combined Approach in a Non-Screened Population

2006 ◽  
Vol 21 (3) ◽  
pp. 149-156 ◽  
Author(s):  
I. Marchetti ◽  
K. Zavaglia ◽  
G. Bertacca ◽  
P. Aretini ◽  
B. Matteoli ◽  
...  
Keyword(s):  
Pap Test ◽  
Hpv Infection ◽  
Low Grade ◽  
Cytological Diagnosis ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
Dot Blot ◽  
Screening Programs ◽  
Hpv Test ◽  
Intraepithelial Lesions

The aim of the present study was to test the polymerase chain reaction (PCR) as a tool to identify human papillomavirus (HPV) in routine cytological samples scraped from the uterine cervix. Moreover, attention has been focused on the correlation between HPV types and early intraepithelial lesions. The study involved 586 women who had undergone conventional Pap test. Analysis of HPV infection was performed by PCR and HPV typing by dot blot. In a group of 78 cases histologically diagnosed as high-grade squamous intraepithelial lesions (HSILs), the cytological diagnosis was correct in 92.3% and the HPV test was positive in 89.8% of cases; combined positivity at Pap and/or HPV tests raised this figure to 99.0%. In a group of 67 cases histologically diagnosed as low-grade squamous intraepithelial lesions (LSILs), the cytological diagnosis was correct in 73.1% and the PCR-based HPV test was positive in 64.2%; combined positivity at Pap and/or HPV tests raised this figure to 91.0%. This study confirms the limitations of screening programs based on Pap test only. Our results suggest, in fact, that adding the HPV test to primary screening could increase the yield of preinvasive cervical lesions.

Human papillomavirus–specific viral types are common in Mexican women affected by cervical lesions

2006 ◽  
Vol 16 (3) ◽  
pp. 1041-1047
Author(s):  
P. Piña-Sánchez ◽  
D. M. Hernández-Hernández ◽  
R. LÓPEZ-ROMERO ◽  
G. VÁZQUEZ-ORTÍZ ◽  
C. PÉREZ-PLASENCIA ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
Hpv 16 ◽  
Female Population ◽  
Hpv Types ◽  
Intraepithelial Lesions ◽  
Hpv 18

Cervical cancer (CC) is the most common in Mexican female population. The human papillomavirus (HPV) 16 and 18 frequencies in worldwide may be different due to geographical distribution. We analyzed the prevalence of HPV types and determinated their association in cervical lesion in a Mexican population. One hundred fifty-nine normal cervical smears, 95 low-grade squamous intraepithelial lesions (LGSIL), 59 high-grade squamous intraepithelial lesions (HGSIL), and 108 CC samples of the patients were collected. HPV types were determined by sequencing. We detected 11 high-risk types, four low-risk types, three not determinated, and two probably high risk. HPV were present in 12%, 57%, 88%, and 92% from normal, LGSIL, HGSIL, and CC samples, respectively. HPV 16 was the most common in all cervical lesions (71.6% in CC). HPV 58 was present in 18.6% of HGSIL, and the HPV 18 in 4.6% of CC. The 76% of all detected viruses belong to A9 species branch. Control women showed high percentage of HPV high-risk infection, suggesting that this is a high-risk group. High frequency of HPV 16 compared with a low incidence of HPV 18 was observed. HPV 58 is frequently detected in HGSIL but low frequency is found in CC. These findings might be considered for HPV screening.

Extracellular regulated kinase-2 immunoreactivity increases in parallel with cervical intraepithelial neoplasia grade in cervical neoplasia

2008 ◽  
Vol 18 (3) ◽  
pp. 540-545
Author(s):  
V. KAPETANIOS ◽  
A. C. LAZARIS ◽  
P. BOGRIS ◽  
S. KOUNELI ◽  
A. NONNI ◽  
...  
Keyword(s):  
Cervical Intraepithelial Neoplasia ◽  
Mapk Pathway ◽  
Statistical Significance ◽  
Intraepithelial Neoplasia ◽  
Early Event ◽  
Low Grade ◽  
High Grade ◽  
Cervical Lesions ◽  
Cin Iii ◽  
Extracellular Regulated Kinase

The cell cycle control system includes cyclins, cyclin-dependent kinases (CDK), and their inhibitors (CDK1). Extracellular regulated kinase (ERK1/2) (p44 and p42 mitogen-activated protein kinases [MAPKs]) is a component of the MAPK pathway, which is associated with cyclin D1 and CDK. It is a critical signaling system for the induction of cell proliferation, differentiation, and cell survival. The aim of this study was to investigate the usefulness of ERK2 expression as a marker of biological aggressiveness complementary to cervical intraepithelial neoplasia (CIN) grade as well as to compare its expression in preinvasive lesions with that in invasive carcinoma. Paraffin-embedded sections of 146 CIN lesions (32 CIN I, 49 CIN II, and 43 CIN III) and 22 invasive cervical carcinomas (13 squamous and 9 adenocarcinomas) were used for the standard immunohistochemical procedure with the application of the ERK2 monoclonal antibody. ERK2 staining displayed a cytoplasmic and nuclear pattern. The staining intensity was gradually increased according to the severity of the dysplastic lesions; ERK2 immunoreactivity was significantly increased in high-grade dysplastic lesions (CIN II and CIN III) and invasive carcinomas by comparison to low-grade dysplastic lesions (CIN I) (P < 0.001). When high-grade lesions were separately assessed, the differences between each one of them and CIN I retained their statistical significance: CIN II versus CIN I (P < 0.001) and CIN III versus CIN I (P < 0.001). In conclusion, our study found a direct relationship between the increasing grade of the dysplastic cervical lesions and the intensity of ERK2 staining, thus implying a role of ERK2 as an early event in cervical carcinogenesis.

scholarly journals Protein Phosphorylation in Serine Residues Correlates with Progression from Precancerous Lesions to Cervical Cancer in Mexican Patients

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Juan Ramón Padilla-Mendoza ◽  
Arturo Contis-Montes de Oca ◽  
Mario Alberto Rodríguez ◽  
Mavil López-Casamichana ◽  
Jeni Bolaños ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Protein Phosphorylation ◽  
Precancerous Lesions ◽  
Human Papillomaviruses ◽  
Serine Phosphorylation ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
Phosphorylated Proteins ◽  
Intraepithelial Lesions

Protein phosphorylation is a posttranslational modification that is essential for normal cellular processes; however, abnormal phosphorylation is one of the prime causes for alteration of many structural, functional, and regulatory proteins in disease conditions. In cancer, changes in the states of protein phosphorylation in tyrosine residues have been more studied than phosphorylation in threonine or serine residues, which also undergo alterations with greater predominance. In general, serine phosphorylation leads to the formation of multimolecular signaling complexes that regulate diverse biological processes, but in pathological conditions such as tumorigenesis, anomalous phosphorylation may result in the deregulation of some signaling pathways. Cervical cancer (CC), the main neoplasm associated with human papillomavirus (HPV) infection, is the fourth most frequent cancer worldwide. Persistent infection of the cervix with high-risk human papillomaviruses produces precancerous lesions starting with low-grade squamous intraepithelial lesions (LSIL), progressing to high-grade squamous intraepithelial lesions (HSIL) until CC is generated. Here, we compared the proteomic profile of phosphorylated proteins in serine residues from healthy, LSIL, HSIL, and CC samples. Our data show an increase in the number of phosphorylated proteins in serine residues as the grade of injury rises. These results provide a support for future studies focused on phosphorylated proteins and their possible correlation with the progression of cervical lesions.

HPV16 E7 Oncoprotein As a Predictor of Diagnosis and Prognosis  in Patients with Cervical Lesions

2020 ◽  
Author(s):  
Guangnan Shuai ◽  
Junhui Xu ◽  
Meihua Liu ◽  
Xueping Zhu ◽  
Na Zhang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Critical Value ◽  
Cervical Lesion ◽  
Pathological Examination ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
E7 Oncoprotein ◽  
The Third ◽  
Intraepithelial Lesions

Abstract Backgroud：The early diagnosis and treatment of HSIL is a key measure to prevent the occurrence of cervical cancer. Although the methods of cervical cancer screening are becoming more and more abundant, some patients still have unnecessary colposcopy referrals. This study aim to explore the value of human papillomavirus 16 (HPV16) E7 oncoproteinin cervical lesion screening and risk assessment of the prognosis for more effective colposcopy. Method: HPV16 E7 oncoprotein in cervical exfoliated cells was detected by using E7 Oncoprotein (HPV16) Diagnostic Kit (Magnetic Partical Chemiluminescence Method). In the first part, HPV16 E7 oncoprotein in different degrees of cervical lesions was retrospectively compared to find the best critical value; In the second part, the value of this test was verified ; In the third part, the women diagnosed as low-grade squamous intraepithelial lesions (LSIL) or normal were followed up for 3 years and the outcomes were compared. Results: In the first part, the expression of HPV16 E7 oncoprotein was positively correlated with the degree of cervical lesion; The critical value determined by ROC curve is 8.68ng/ml, which is accurate in the diagnosis of high-grade squamous intraepithelial lesions (HSIL) and invasive carcinoma of cervix (CA); In the second part, there were higher sensitivity(87.1) and specificity(70) for E7 oncoprotein; HPV16 E7 oncoprotein has higher consistency with pathological examination in detection of HSIL or cervical cancer（0.573 vs 0.369) than TCT; In the third part，HPV16 E7 oncoprotein has a high positive predictive value (82.4%) and positive likelihood ratio (4.43) for the prognosis of patients with LSIL and below. Conclusions: The status of HPV16E7 oncoprotein shows important clinical value for the detection and prediction of cervical lesions. Patients with positive HPV16 E7 oncoprotein are more likely to develop the disease and have a higher risk of disease progression.

scholarly journals Do Infection Patterns of Human Papillomavirus Affect the Cytologic Detection of High-Grade Cervical Lesions on Papanicolaou Tests?

2017 ◽  
Vol 142 (3) ◽  
pp. 347-352 ◽  
Author(s):  
Siavash Azadmanesh Samimi ◽  
Roxanne R. Mody ◽  
Steven Goodman ◽  
Eric Luna ◽  
Donna Armylagos ◽  
...  
Keyword(s):  
Hpv Infection ◽  
Low Grade ◽  
Lower Grade ◽  
High Grade ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
Atypical Squamous Cell ◽  
Intraepithelial Lesion ◽  
Intraepithelial Lesions ◽  
Hpv 18

Context.— Persistent infection with high-risk human papillomavirus (hrHPV) is the major cause of cervical cancer. The effect of HPV infection patterns on cytologic detection of cervical lesions is unknown. Objective.— To determine the effect of HPV infection patterns on the sensitivity of cytologic detection of high-grade cervical lesions. Design.— Papanicolaou tests from 257 women with biopsy-confirmed, high-grade squamous intraepithelial lesions were analyzed with respect to HPV infection patterns. Results.— Among 257 biopsy-confirmed, high-grade squamous intraepithelial lesion cases, the preceding cytology showed 20 cases (8%) were benign; 166 cases (65%) were low-grade cervical lesions, including atypical squamous cell of undetermined significance and low-grade squamous intraepithelial lesions; and 71 cases (28%) were high-grade cervical lesions, including atypical squamous cells cannot rule out high-grade squamous intraepithelial lesion (atypical squamous cell–high), atypical glandular cells, and high-grade squamous intraepithelial lesions. In 236 cases tested for HPV, those exhibiting low-grade cervical lesions on cytology were often associated with coinfections of mixed hrHPV genotypes (31 of 40; 78%) or non-16/18 hrHPV (75/103; 73%), compared with single-genotype infections of HPV-16 (33 of 62; 53%) or HPV-18 (2 of 6; 33%) (P = .001). In contrast, high-grade cervical lesion cytomorphology tended to associate with the single-genotype infection of HPV-16 (20 of 62; 32%) or HPV-18 (3 of 6; 50%), compared with non-16/18 hrHPV (25 of 103; 24%) or multigenotype infection (8 of 40; 20%) (P = .01). Conclusions.— Our findings suggest that multigenotypic or non-16/18 hrHPV infections often produce deceptive lower-grade cytomorphology, which could result in underdiagnosis and delay of treatment. The HPV infection patterns may offer unrecognized benefit beyond HPV genotyping and should be considered during clinical risk evaluation of women with lower-grade cytology.

scholarly journals Abnormal Pap Smear Results in Outpatient Attendees in a Tertiary Care Center in Kathmandu, Nepal

2017 ◽  
Vol 16 (1) ◽  
pp. 3-7
Author(s):  
Ratna Adhikari Khatri ◽  
Lee Budhathoki ◽  
Arya Rana Pande ◽  
Sadikshya Singh
Keyword(s):  
Epithelial Cell ◽  
Squamous Cell ◽  
Pap Smear ◽  
Care Center ◽  
Tertiary Care ◽  
Pap Test ◽  
Intraepithelial Neoplasia ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Intraepithelial Lesions

Introduction: Cervical cancer is one of the most common cancers in the world. It is the most common cancer among females in Nepal. Progression of pre-invasive precursor lesions called Cervical Intraepithelial Neoplasia (CIN) can result in invasive cancer over years and decades. Papanicolaou smear can detect these lesions early. The aim of this paper is to study the prevalence of abnormal Pap smear test and report the results.Methods: Observational study of Pap smear of 1100 women of age 21 to 65 years attending Gynaecological outpatient department of our hospital over a period of one year.Results: Pap smear results were recorded as per Bethesda system. 35% of smears had epithelial cell abnormality with 62.82% normal smear and 2.13%infections, bacterial vaginosis (1.94%) being most common. The highest epithelial cell abnormality was Low grade squamous intraepithelial lesions (29.90%) followed by Atypical squamous cell of undetermined significance (3.2%). High grade squamous intraepithelial lesions and carcinoma were next to follow (0.97% each). 80% of cancers were Squamous cell carcinoma.Conclusion: Premalignant and malignant lesions were found to be common in cervix. Pap test is simple, quick and effective way of screening them. Screening should be done at least once for all woman after the age of 30 as benefit is maximum in this age group.

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