Establishment of cellulolytic bacteria in the digestive tract of conventionally reared young mice: effect of the dietary cellulose content in the adult

On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate (µ) 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).How to CiteWijanarka, W., Kusdiyantini, E. & Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 386-392.

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KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL

ALCHEMY ◽

10.18860/al.v0i0.2297 ◽

2013 ◽

Cited By ~ 2

Author(s):

Dyah Ayu Saropah ◽

Akyunul Jannah ◽

Anik Maunatin

Keyword(s):

Rice Bran ◽

Enzymatic Reaction ◽

Milling Process ◽

Cellulolytic Enzyme ◽

Cellulose Content ◽

Optimum Conditions ◽

Cellulolytic Bacteria ◽

Cellulase Enzymes ◽

Rice Milling

Bran rice is a by-product of rice into rice milling process, the cellulose content of 40-60%, so the potential as a carbon source for the growth of microorganisms such as bacteria to produce enzymes particularly cellulolytic bacteria. The purpose of the study was to determine the diversity of the characters from the cellulolytic bacterial isolates and optimum conditions enzyme (cellulase enzymes rough) so that they can hydrolyze the cellulose to glucose with either rice bran. The characterization includes the determination of pH, temperature and time of optimum crude extract of bacterial cellulolytic enzyme cellulase, determination of Vmax and Km and molecular mass determination of cellulase.Research methods include making media, regeneration of isolates, bacterial growth curve manufacturing, production of cellulase enzymes from bacterial cellulolytic rough at the optimum conditions, the kinetics of enzymatic reaction: substrate concentration factor of the reaction rate (with variation of the concentration of 0.50%, 0.75%, 1 , 00%, 1.25% and 1.50% (w / v)) followed by calculating the Vmax and Km.The results showed that the enzyme cellulase of cellulolytic bacteria isolated from rice bran result that has optimum conditions at pH 7.5, temperature 50 ° C, 40 min incubation time to produce Vmax 0.0086 units / mL and Km 1.694%.

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Endoglucanase activity of cellulolytic bacteria from lake sediments and its application in hydrophyte degradation

FEMS Microbiology Letters ◽

10.1093/femsle/fnaa200 ◽

2020 ◽

Author(s):

Hongpei Zhang ◽

Qianzheng Li ◽

Yuqing Zhao ◽

Mingzhen Zhang ◽

Dong Xu ◽

...

Keyword(s):

Degradation Rate ◽

Hydrilla Verticillata ◽

Aquatic Organisms ◽

Plant Residue ◽

Plant Residues ◽

Cellulose Content ◽

Cellulolytic Bacteria ◽

Bacterial Strains ◽

Submerged Plants ◽

Endoglucanase Activity

Abstract Hydrophytes are plants that grow in or on water. Their overgrowth adversely affects the ecosystem because of crowding out other aquatic organisms and polluting the environment with plant residue. In principle, cellulolytic bacteria can be used to degrade hydrophyte biomass. We here isolated and characterized four cellulolytic bacterial strains from Lake Donghu sediments (Wuhan, China) that are rich in organic matter and plant residues. The isolates (WDHS-01 to 04) represent Bacillus, Micromonospora, and Streptomyces genera. The bacteria exhibited pronounced endoglucanase activities (from 0.022 to 0.064 U mL–1). They did not extensively degrade the emerged plant Canna indica L. However, in an Hydrilla verticillata (submerged plant) degradation medium, strain WDHS-02 exhibited a high degradation rate (54.91%), endoglucanase activity of 0.35 U mL–1 and the conversion rate of cellulose to reducing sugars of 7.15%. Correlation analysis revealed that bacterial endoglucanase activity was significantly correlated with the degradation rate, and acid detergent lignin, ash and cellulose content of the residual H. verticillata powder. In conclusion, the identified bacteria efficiently decomposed submerged plants without the need for acid–base pretreatment. They expand the set of known cellulolytic bacteria and can be used for natural degradation of submerged plants.

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Isolation of Cellulolytic Bacterium Staphylococcus sp. JC20 from the Intestine of Octopus (Octopus sp.) for Fish Probiotic Candidate

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.39525 ◽

2019 ◽

Vol 21 (2) ◽

pp. 93 ◽

Cited By ~ 1

Author(s):

Indah Istiqomah ◽

Imelda Novita Atitus ◽

Ahmad Fauzi Rohman ◽

Alim Isnansetyo

Keyword(s):

Digestive Tract ◽

Agar Plate ◽

Acid Resistance ◽

Cellulolytic Bacterium ◽

Cellulolytic Activity ◽

Cellulolytic Bacteria ◽

Fish Pathogens ◽

Antagonist Activity ◽

16S Rdna Gene ◽

Antibiotics Sensitivity

Aim of this study was to isolate, characterize, and identify cellulolytic bacteria from the digestive tract of marine vertebrates and invertebrates as a candidate of fish probiotics. The bacteria were isolated from the digestive tract and grown on a cellulose agar plate. The bacteria were screened based on the cellulolytic activity, acid resistance, antagonist activity against fish pathogens, antibiotics sensitivity, ability to live in fish digestive tract and non-pathogenic test. Selected bacterium was identified molecularly, based on the 16S rDNA gene sequences, and phenotipically. A total of 14 bacteria demonstrated celulolitic index of 1.1-1.8. The bacteria with cellulolytic index of > 1.6 were screened by the selection criteria, resulted a selected strain, JC20 isolate which was isolated from the digestive tract of octopus (Octopus sp.). The selected bacterium was sensitive to antibiotics, resists to acidic environment, able to live in the fish digestive tract, and non-pathogen. Thus, the bacterium was potential for further characterization as fish probiotics candidate. Molecular and phenotypic identification revealed that JC20 isolate was Staphylococcus sp.

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Characterization of the microbial and biochemical profile of the different segments of the digestive tract in horses given two distinct diets

Animal Science ◽

10.1017/s1357729800059038 ◽

2003 ◽

Vol 77 (2) ◽

pp. 293-304 ◽

Cited By ~ 74

Author(s):

A. de Fombelle ◽

M. Varloud ◽

A.-G. Goachet ◽

E. Jacotot ◽

C. Philippeau ◽

...

Keyword(s):

Lactic Acid ◽

Digestive Tract ◽

Large Intestine ◽

Volatile Fatty Acids ◽

Anaerobic Bacteria ◽

Average Concentration ◽

Cellulolytic Bacteria ◽

Fibre Degradation ◽

Fermentable Carbohydrates ◽

Pelleted Food

AbstractA first group of three horses was given diet 1 (D1) allowing 1180 g per 100 kg body weight (BW) of a pelleted food rich in fibre (P1) and 556 g per 100 kg BW of straw during a 20-day period to allow for adaptation. A second group of four horses were given diet 2 (D2) allowing 1180 g per 100 kg BW of a pelleted food rich in cereals (P2) and 1000 g per 100 kg BW of meadow hay during the same period. Digesta was collected from the stomach, duodenum, jejunum, ileum, caecum, right ventral colon, left ventral colon, left dorsal colon, right dorsal colon, and small colon, and faeces were collected under general anaesthesia 2·5 h after the ingestion of the morning pelleted meal. The concentration of total anaerobic, cellulolytic and lactic acid-utilizing bacteria, lactobacilli and streptococci were determined in all these segments except for the duodenum, left ventral colon, right dorsal colon and small colon. D-/L-lactic acid, volatile fatty acids and pH were measured in all anatomic segments of the digestive tract (from stomach to small colon). The caecal concentration of total anaerobic bacteria was the lowest (7·9 5 107colony-forming units (c f. u.) per ml), whereas that of the stomach was the highest (1·4 5 109c f. u. per ml) (P< 0·001). Cellulolytic bacteria did not exceed 3·0 5 102c f u. per ml in the ante-caecal segments whereas in the hindgut the average concentration was 5·3 x 105c f u. per ml (P< 0·001). Likewise, VFA concentrations were also greater in the large intestine (on average, 96·3 mmol/l v. 8·8 mmol/l in the ante-caecal segments) (P< 0·001), confirming the limited extent of fibre degradation in these ante-caecal segments. Lactobacilli, streptococci and lactate-utilizing bacteria colonized all the digestive tract; the stomach and the small intestine tended to host the greatest numbers of these bacteria, which suggests a high interference of micro-organisms with the digestion of readily fermentable carbohydrates. Compared with the other ante-caecal segments, the stomach ecosystem seemed the most affected by the composition of the last pelleted meal ingested: the concentrations of lactobacilli and lactate-utilizing bacteria were higher (P< 0·05) with P2. The lower concentration of D-/L-lactate with P2 (P< 0·05) was concomitant with a greater proportion of propionate (P< 0·05), probably related to a greater fermentation of lactate. In the large intestine of horses given D2, cellulolytic bacteria tended to be lower, whereas VFA concentrations were higher (P < 0·05). The lower [NDF/starch] ratio of D2 was probably less propitious for the proliferation of cellulolytic bacteria but was compensated by the higher cellulose intake brought by the hay.

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Kelimpahan Bakteri Selulolitik di Muara Sungai Gunung Anyar Surabaya dan Bancaran Bangkalan [The Total of Cellulolytic Bacteria in Gunung Anyar Surabaya and Bancaran Bangkalan Estuaries ]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v6i2.11299 ◽

2019 ◽

Vol 6 (2) ◽

pp. 143

Author(s):

Didya Sinatryani, Moch. Amin Alamsjah Sudarno, Kustiawan Tri Pursetyo

Keyword(s):

Organic Compounds ◽

Mangrove Ecosystem ◽

Soil Sampling ◽

Plate Count ◽

Cellulose Content ◽

Cellulolytic Bacteria ◽

Total Plate Count ◽

Degrading Bacteria ◽

Organic Particles ◽

Number Of Bacteria

Abstract Most organic materials utilized mangrove detritus such as mangrove leaves fall throughout the year. Organic particles or litter into a place to live for bacteria, fungi and other microorganisms. One of organic compounds in the soil is cellulose. Deciduous leaves on the ground allows that the cellulose content in the soil is high, it is possible to find cellulose degrading bacteria in the mangrove ecosystem. Soil sampling conducted in April 2014 located in Gunung Anyar Surabaya estuaries and Bancaran Bangkalan estuaries. After taking the samples, the isolation of cellulolytic bacteria and bacteria calculation were conduct using standard Total Plate Count (TPC). Based on the results of the calculation of total number bacteria, obtained the highest total number of cellulolytic bacteria at station E (Bancaran) of 4.9 x 104 CFU/ml. The highest percentage of cellulolytic bacteria obtained at station D (Bancaran) with a percentage of 27.09%. According to the whole calculation of the total number of bacteria, total number and percentage of cellulolytic bacteria, it was found that the area of Bancaran Bangkalan has higher abundance of cellulolytic bacteria than Gunung Anyar Surabaya mangrove areas.

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