scholarly journals Kelimpahan Bakteri Selulolitik di Muara Sungai Gunung Anyar Surabaya dan Bancaran Bangkalan [The Total of Cellulolytic Bacteria in Gunung Anyar Surabaya and Bancaran Bangkalan Estuaries ]

2019 ◽  
Vol 6 (2) ◽  
pp. 143
Author(s):  
Didya Sinatryani, Moch. Amin Alamsjah Sudarno, Kustiawan Tri Pursetyo
Keyword(s):  
Organic Compounds ◽  
Mangrove Ecosystem ◽  
Soil Sampling ◽  
Plate Count ◽  
Cellulose Content ◽  
Cellulolytic Bacteria ◽  
Total Plate Count ◽  
Degrading Bacteria ◽  
Organic Particles ◽  
Number Of Bacteria

Abstract Most organic materials utilized mangrove detritus such as mangrove leaves fall throughout the year. Organic particles or litter into a place to live for bacteria, fungi and other microorganisms. One of organic compounds in the soil is cellulose. Deciduous leaves on the ground allows that the cellulose content in the soil is high, it is possible to find cellulose degrading bacteria in the mangrove ecosystem. Soil sampling conducted in April 2014 located in Gunung Anyar Surabaya estuaries and Bancaran Bangkalan estuaries. After taking the samples, the isolation of cellulolytic bacteria and bacteria calculation were conduct using standard Total Plate Count (TPC). Based on the results of the calculation of total number bacteria, obtained the highest total number of cellulolytic bacteria at station E (Bancaran) of 4.9 x 104 CFU/ml. The highest percentage of cellulolytic bacteria obtained at station D (Bancaran) with a percentage of 27.09%. According to the whole calculation of the total number of bacteria, total number and percentage of cellulolytic bacteria, it was found that the area of Bancaran Bangkalan has higher abundance of cellulolytic bacteria than Gunung Anyar Surabaya mangrove areas.

scholarly journals Verification of prediction of growth of Listeria monocytogenes micro-organism in chicken meat

2000 ◽  
Vol 18 (No. 5) ◽  
pp. 183-186
Author(s):  
A. Landfeld ◽  
M. Karpíšková R Houška ◽  
K. Kýhos ◽  
P. Novotná
Keyword(s):  
Listeria Monocytogenes ◽  
Incubation Temperature ◽  
Chicken Meat ◽  
Raw Material ◽  
Plate Count ◽  
Raw Meat ◽  
Total Plate Count ◽  
Measured Water ◽  
Number Of Bacteria ◽  
Storage Temperatures

Raw chicken meat was comminuted and homogenised. There were measured water activity and pH (aw = 1 for temperature 25°C, pH = 5.8 for temperature 8°C). Input raw material was investigated for the presence of Listeria monocytogenes (negative) and the raw meat was inoculated by Listeria monocytogenes CCM 4699. Number of Listeria monocytogenes, total plate count and number of coliforms were determined in the range 0–7 days by bacteriological survey for the storage temperatures 4.9, 7 and 8.3°C. The increase of Listeria monocytogenes counts has been registered for temperature 4.9°C about log 1.5 CFU/g after 6 days, for temperatures 7 and 8.3°C about 2 log CFU/g (regarding to the starting counts). The prediction for listeria growth with the aid of Food MicroModel was also made. The best agreement between the experimentally analysed number of bacteria and prediction was received for the lowest incubation temperature 4.9°C.

scholarly journals TOTAL BAKTERI DAN IDENTIFIKASI Escherichia coli PADA JAJANAN SIOMAY IKAN YANG DIJAJAKAN DI BEBERAPA SD NEGERI DI KOTA KENDARI

2019 ◽  
Vol 2 (2) ◽  
pp. 196
Author(s):  
Wa Ode Rusmianur ◽  
Asnani Asnani ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Escherichia Coli ◽  
Random Sampling ◽  
Sampling Method ◽  
Food Poisoning ◽  
Plate Count ◽  
Foodborne Disease ◽  
Total Plate Count ◽  
Fish Samples ◽  
Descriptive Survey ◽  
Number Of Bacteria

ABSTRACT          Contamination in food may cause foodborne disease, one of them are diarrhea and food poisoning. The cause of contamination in food is microbial contamination. This study aims to determine the presence of contamination Escherichia coli and the number of bacteria on fish siomay, being sold at public elementary school in Kendari city (Kendari  barat, Mandonga, Puwatu and Poasia). This Research used a descriptive survey with random sampling  method to determine the number of bacteria and Escherichia coli using total plate count (TPC) and EMBA medium. The results showed that the number of bacteria were (A) 3,50 x 103CFU/gram, (B) 3,00 x 103 CFU/gram,  (C) 6,67 x 103 CFU/gram dan (D) 8,00 x 103CFU/gram and wasfound Escherichia coli in samples A, C and D, while samples B not found Escherichia coli. This research showed that 3 out of 4 siomay fish samples (80%) contain Escherichia coli and the number of bacteria still according to SNI standar and siomay is still suitable for consumption.Keywords: Escherichia coli. Foodborne disease, Siomay, Total Plate Count (TPC).ABSTRAKAdanya kontaminasi pada pangan jajanan dapat menyebabkan foodborne disease, salah satunya adalah diare dan keracunan pangan. Penyebab kontaminasi pada pangan adalah cemaran mikroba. Penelitian ini bertujuan untuk mengetahui adanya cemaran  Escherichia coli dan jumlah bakteri pada jajanan siomay ikan yang dijajakan di Beberapa Sekolah Dasar Negeri di Kota Kendari (kecamatan Kendari barat, Mandonga, Puwatu dan Poasia). Metode penelitian yaitu Survey deskriptif dengan pengambilan sampel secara acak (random sampling) untuk mengetahui jumlah bakteri dan adanya bakteri Escherichia coli pada makanan jajanan siomay ikan dengan menggunakan metode TPC dan isolasi pada media EMBA. Hasil Penelitian menunjukan rata-rata jumlah bakteri yaitu (A) 3,50 x 103CFU/gram, (B) 3,00 x 103 CFU/gram,  (C) 6,67 x 103 CFU/gram dan (D) 8,00 x CFU/gram ditemukan Escherichia coli pada sampel A, C dan D sedangkan sampel B tidak ditemukan bakteri Escherichia coli. Hal iniMenunjukkan bahwa 3 dari 4 sampel siomay telah tercemar bakteri Escherichia coli sebesar 80% dengan total koloni bakteri masih memenuhi syarat yang ditetapkan SNI siomay ikan dan masih layak dikonsumsi.Kata kunci: Escherichia coli, Foodborne disease, Siomay, Total Plate Count (TPC).

scholarly journals Pengaruh Lama Pemakaian Sediaan Kosmetik Bedak Padat Terhadap Cemaran Mikroba

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Munira Munira ◽  
Cut Fardilla ◽  
Noni Zakiah ◽  
Rasidah Rasidah ◽  
Muhammad Nasir
Keyword(s):  
Bacterial Contamination ◽  
Plate Count ◽  
Test Results ◽  
Food Control ◽  
Average Value ◽  
Total Plate Count ◽  
Compact Powder ◽  
Long Time ◽  
The Republic ◽  
Number Of Bacteria

Bedak adalah salah satu sediaan kosmetik yang dipakai dalam jangka waktu yang lama. Bedak yang digunakan akan terjadi kontaminasi bahkan semakin meningkat seiring dengan lamanya pemakaian. Faktor-faktor yang mempengaruhi antara lain penyimpanan, lingkungan, dan spons bedak. Penggunaan spons bedak merupakan salah satu sumber cemaran bakteri. Di mana penggunaan spons yang sama secara berulang-ulang dan bersentuhan langsung pada kulit dapat meningkatkan jumlah mikroba. Tujuan penelitian ini adalah untuk mengetahui pengaruh lamanya penyimpanan, pemakaian suatu sediaan kosmetik bedak padat terhadap tingkat cemaran mikroba. Sampel yang diuji adalah bedak padat yang belum dipakai dan yang telah dipakai selama 2 minggu. Hasil penelitian berupa jumlah cemaran bakteri (ALT) dibandingkan dengan syarat menurut Peraturan Kepala Pengawasan Obat dan Makanan RI No.HK.03.1.23.07.11.6662 Tahun 2011 yaitu tidak boleh melebihi 103 koloni/g. Hasil uji cemaran bakteripada sediaan bedak padat sebelum pemakaian mempunyai nilai rata-rata ALT 2,6 x 101koloni/g dan sesudah pemakaian mempunyai nilai rata-rata ALT 9,2 x 101 koloni/g. Nilai ALT yang diperoleh dari masing-masing sediaan kosmetik bedak padat telah memenuhi syarat.Hasil uji t dependen menunjukkan adanya perbedaan jumlah bakteri pada bedak padat sebelum dipakai dengan setelah dipakai selama 2 minggu (t = 0.000). Kata Kunci: Kosmetik, bedak padat, Angka Lempeng Total ABSTRACTPowder is one of the cosmetic preparations used for a long time. Powder that is used will occur contamination even increasing along with the length of usage. This can be caused by several factors including storage, environment, powder sponges and others. The use of powder sponges is one source of bacterial contamination. Where the use of the same sponge repeatedly and in direct contact with the skin can increase the number of microbes. The objective of study was to determine the effect of the duration of use of a compact powder cosmetic preparation on the level of microbial contamination. The samples tested were solid powder that has not been used and that has been used for 2 weeks. The results of the study were the Total Plate Count (TPC) compared to the Regulation of the Head of the Republic of Indonesia Drug and Food Control Number HK.03.1.23.07.11.6662 of 2011 concerning the Requirements for Microbial Pollution and Heavy Metals in Cosmetics which should not exceed 103 colonies / g. The test results of bacterial contamination on solid powder preparations before use have an average value of ALT 2,6 x 101 colony / g and after use have an average ALT value of 9.2 x 101 colony / g. The ALT value obtained from each compact powder cosmetic preparation has met the requirements. The results of the dependent t test showed a difference in the number of bacteria on solid powder before use with after being used for 2 weeks (t = 0.000). Keywords: Cosmetics, Compact Powder, Total Plate Count

scholarly journals Aplikasi Kitosan Emerita sp. Sebagai Bahan Pengawet Alternatif pada Ikan Belanak (Mugil cephalus) [Chitosan Emerita sp. as a Preservative Alternative in Mugil cephalus]

2019 ◽  
Vol 11 (1) ◽  
pp. 34
Author(s):  
Khoeruddin Wittriansyah ◽  
Soedihono Soedihono ◽  
Dodi Satriawan3
Keyword(s):  
Acetic Acid ◽  
Lower Number ◽  
Mugil Cephalus ◽  
Plate Count ◽  
Chitosan Concentration ◽  
Organoleptic Test ◽  
Total Plate Count ◽  
Number Of Bacteria

AbstrakEmerita sp. dapat diolah kitosan sebagai alternatif bahan pengawet ikan. Tujuan penelitian ini adalah untuk mengetahui aktivitas kitosan Emerita sp. dalam menghambat pertumbuhan mikroorganisme pembusuk pada ikan sehingga dapat digunakan sebagai bahan pengawet alternatif. Sampel Emerita sp. diperoleh dari pesisir pantai Widarapayung,Cilacap. Karakteristik kitosan Emerita sp. dianalisa melalui uji FTIR dan uji proksimat. Aktivitas kitosan Emerita sp. sebagai pengawet ikan, dianalisa melalui uji organoleptik dan uji Total Plate Count (TPC). Ikan belanak direndam dalam larutan kitosan Emerita sp. pada konsentrasi 0,5%, 1,5%, dan 2%. Lama waktu perendaman yaitu 15 menit, 30 menit dan 60 menit. Pengamatan kemunduran mutu dilakukan pada jam ke 0, 10, 15 dan ke 24. Kontrol menggunakan asam asetat 1% dengan perendaman selama 15 menit. Hasil penelitian menunjukkan Nilai Derajat Deasetilisasi (DD) kitosan Emerita sp. adalah 92,5%. Hasil terbaik uji organoleptik ditunjukan pada kosentrasi kitosan 0,5 % dengan lama perendaman selama 60 menit dibandingkan kontrol. Uji (TPC) menunjukkan ikan dengan perendaman kitosan Emerita sp. 2% selama 60 menit, memiliki jumlah bakteri lebih rendah (2,7x106) daripada kontrol (3,2 x106). Bedasarkan hasil uji TPC dan Organoleptik, kitosan Emerita sp. memiliki aktivitas penghambat pertumbuhan mikroorganisme sehingga dapat digunakan sebagai alternatif bahan pengawet.AbstractEmerita sp. can be processed into chitosan as an alternative to fish preservatives. The purpose of this study was to determine chitosan from Emerita sp. in inhibiting the growth of decomposing microorganisms in fish so it can be used as alternative preservatives. Emerita sp. was obtained from the coast of Widarapayung, Cilacap. Characteristics of chitosan from Emerita sp. was analyzed through FTIR profileand proximate content. The activity of chitosan from Emerita sp. as a fish preservative, analyzed through organoleptic and total plate count (TPC) test. Bluespot mullet fish was soaked in chitosan from Emerita sp. at concentrations of 0.5%, 1.5%, and 2%. Soaking process took was 15, 30 and 60 minutes. Observations on fish decay was conducted at the hour of 0, 10, 15 and 24. Control used 1% of acetic acid with soaking process for 15 minutes. The results of the study showed that the degrees of deacetylization (DD) chitosan from Emerita sp. is 92.5%. The best results of organoleptic were shown on 0.5% chitosan concentration with 60 minutes soaked time compared to controls. TPC shows fish with the soaking process in chitosan Emerita sp. 2% for 60 minutes, having a lower number of bacteria (2,7x106) than the control (3,2 x106). Based on the results of the TPC and organoleptic test, chitosan Emerita sp. has activity inhibiting the growth of microorganisms so that it can be used as an alternative preservative.

scholarly journals Production and Application of Lactobacillus plantarum IBL-2 Bacteriocins as Meat Product Biopreservatives

2017 ◽  
Vol 14 (1) ◽  
pp. 68
Author(s):  
Marlia Singgih Wibowo ◽  
Isra Muzakiyah ◽  
Betty Nurhayati ◽  
Tjokorde Armina Padmasawitri ◽  
Yantyati Widiastuti ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Proteolytic Enzymes ◽  
Meat Product ◽  
Ground Beef ◽  
Refining Process ◽  
Plate Count ◽  
Total Plate Count ◽  
Three Samples ◽  
Safe Food ◽  
Number Of Bacteria

Biopreservation is one of the alternatives to obtain safe food products. The produced bacteriocin by Lactic Acid Bacteria (LAB) is potential as biopreservatives, which is safe for consumption, since it was a protein degradable by proteolytic enzymes. This study aimed to optimize bacteriocin production from L. plantarum IBL-2 and to evaluate the effectiveness of bacteriocins in reducing the number of total plate count and Salmonella typhimurium in ground beef. Bacteriocin was produced through fermentation of L. plantarum IBL-2, under various conditions to yield the compound with the best antimicrobial activity. The total number of bacteria in ground beef after the addition of L. plantarum IBL-2 fermentation supernatant was determined. The result was compared with the sample without preservatives (control), and sample added with commercial Nissin. All three samples of ground beef were spiked with S. typhimurium and incubated for 0, 2, 6, 8, 12, 14 days at a temperature of 4-10 ° C. Total Plate Count (TPC) method was utilized to determine the number of bacteria in the samples. The fermentation process resulted in bacteriocin with the strongest antimicrobial activity when using low molecular weight liquid medium (LMWLM), followed by a series of refining process. From day 0-14, the number of S. typhimurium, in sample added with L. plantarum IBL-2 fermentation supernatant, was lower than control and sample added with Nissin. The most optimal antimicrobial activity of bacteriocin was obtained using LMWLM as fermentation media, and using a series of refining process consist of bacteriocin supernatant evaporation, membrane ultrafiltration, and gradual fractionation using 80% ammonium sulphate. Bacteriocin from L. plantarum IBL-2 showed antimicrobial activity against S. typhimurium.

scholarly journals IDENTIFIKASI BAKTERI PADA SERASAH DAUN MANGROVE YANG TERDEKOMPOSISI DI BANDAR BAKAU KOTA DUMAI

2020 ◽  
Vol 7 (2) ◽  
pp. 167
Author(s):  
Putri Wening Ratrinia ◽  
Nirmala Efri Hasibuan ◽  
Aulia Azka ◽  
Sumartini Sumartini ◽  
Apri Mujiyanti ◽  
...  
Keyword(s):  
Leaf Litter ◽  
Bacterial Species ◽  
Mangrove Ecosystem ◽  
Morphological Observation ◽  
Plate Count ◽  
Survey Method ◽  
Mangrove Species ◽  
Gram Staining ◽  
Total Plate Count ◽  
Total Bacteria

Bacteria has an important role in decomposition and productivity activities in mangrove ecosystem. The study about identification of decomposing mangrove litter bacteria in Dumai has never been done. The aim of this study is to determine Total Plate Count and identify the dominant bacterial species from decomposing Rizhopora sp, Xylocarpus sp, dan Avicennia sp litter in Dumai. The method used in this research was survey method, bacterial isolation was done by pour plate method which is used in total calculation of bacteria was Total Plate Count (TPC), and identify the dominant bacterial in mangrove litter. The total bacteria which gained on Avicennia sp leaf litter are 0,64 x 105 Cfu/ml. On the other hand, the total bacteria from Rhizopora sp leaf litter has a lower number of colonies that is 0,55 x 105± 0,01 Cfu/ml. The bacterial identification test was carried out by gram staining method and morphological observation by microscopy. The dominant bacterial species in three mangrove species (Rizhopora sp, Xylocarpus sp, dan Avicennia sp) are Micrococcus sp dan Aerococcus sp. Keywords : Total Plate Count, Micrococcus sp, Aerococcus sp, Organic Matterial

scholarly journals Heavy Metal (Pb and Cu) and Bacterial Contamination in Mudskipper, Eeltail Catfish and Mud Clam at Kuala Tambangan Mangrove Ecosystem, South Kalimantan

2020 ◽  
Vol 20 ◽  
pp. 02001
Author(s):  
Anang Kadarsah ◽  
Dafiuddin Salim ◽  
Krisdianto ◽  
Ika Oksi Susilawati
Keyword(s):  
Heavy Metal ◽  
Bacterial Contamination ◽  
Mangrove Ecosystem ◽  
Lead Content ◽  
Plate Count ◽  
Vibrio Cholera ◽  
Total Plate Count ◽  
Mangrove Ecosystems ◽  
Qualitative Test ◽  
The Republic

Heavy metal content (Pb and Cu) in waters and sediments from the Kuala Tambangan mangrove ecosystem, South Kalimantan was above the standard of Minister Environment of the Republic of Indonesia in 2004. Lead content in waters is reached 0.128 mg/L and Cuprum at 0.444 mg/L, but in the sediments is increased dramatically almost 10 times (17,426 mg/gr for Lead and 20,576 mg/gr for Cuprum. The highest content of Lead (0.128 mg/L) was found in the estuary while for Cuprum (0.444 mg/L) found in the interior. The highest value of Lead (17,426 mg/gr) and Cuprum (20,576 mg/gr) for sediments were found in the estuary. Lead content in eeltail catfish and mud clam is can not be detected, but for Mercury (0.1601 mg/kg in eeltail catfish and 0.2653 mg/kg in mud clam) is above the standard of ISO 2354.6-2016. The Total Plate Count values of inhabitants fauna in mangrove ecosystems were below the standard of SNI 2332.3-2015. A qualitative test for Escherichia coli, Salmonella, and Vibrio cholera presents shows a negative value (<3 APM/g). Coliform quantitative test is above the standard of quality (43 APM/gr in eeltail catfish and 150 APM/g in mud clam). The presence of parasites was not found on all samples.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI PENDEGRADASI SELULOSA ASAL EKOSISTEM MANGROVE TUKAK SADAI, BANGKA SELATAN

2018 ◽  
Vol 1 (2) ◽  
pp. 9
Author(s):  
Ardiansyah Kurniawan ◽  
Dwi Febrianti ◽  
Suci Puspita Sari ◽  
Asep Awaludin Prihanto ◽  
Euis Asriani ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Mangrove Ecosystem ◽  
Rrna Gene ◽  
Test Results ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Gram Negative ◽  
Degrading Bacteria ◽  
Cellulose Digestibility

Perikanan dan Kelautan, Universitas Brawijaya3Dosen Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya.ABSTRACTAgricultural waste has problems on fiber and cellulose digestibility in its utilization foraquaculture. The ability of plant cellulose degrading bacteria to become a source ofenergy can increase the digestibility of feed by fish. The purpose of this study was toidentify the cellulolytic bacteria from the mangrove ecosystem, Tukak Sadai District,South Bangka Regency. This study was conducted in March until August 2017.Samples were taken from litter, mud and weathered wood and isolated usingCarboxymetyl Cellulosa (CMC) 1% media and found 22 bacterial isolates. Gramstaining results showed that 3 isolates (TSS 2, TSL 6 and TSK 4) were classified asgram positive and 19 other isolates were gram negative. Cellulolytic test results showed6 isolates had the ability to degrade cellulase, namely 3 isolates from mangrove mudsamples (TSS 1, TSL 7, TSL 1), 2 isolates from leaf litter (TSS 4, TSL 2) and 1 isolatefrom weathered wood (TSK 5) . Sequencing of 16S rRNA gene DNA showed proximityto Pseudomonas aeruginosa in TSL 7 and TSS isolates 4

scholarly journals Growth of Bacillus toyonensis in Tofu Wastewater

2021 ◽  
Vol 934 (1) ◽  
pp. 012021
Author(s):  
N Nursyirwani ◽  
D Yoswaty ◽  
D A Oktavia
Keyword(s):  
Bacterial Growth ◽  
Mangrove Ecosystem ◽  
Plate Count ◽  
Alternative Substrate ◽  
Factors Affecting ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Spectrophotometric Observation ◽  
Plate Counts ◽  
Bacillus Toyonensis

Abstract Bacillus toyonensis has been isolated in Dumai mangrove ecosystem of Riau Province. One of factors affecting the growth of the bacteria is growth substrate. Tofu wastewater is rich in nutrition which can be used as substrate for bacterial growth. This research aimed to observe the growth of B. toyonensis in different concentration of tofu wastewater. The bacteria was grown in tofu wastewater at concentrations 8%, 10% and 12% was supplemented with 0.1 g K2HPO4, 0.15 g KH2PO4, 0.15 g NaCl and 0.5 g vitamin B12 in 100 mL distilled water. The bacterial growth was observed by using spectrophotometer at λ 610 nm and by analysis the total plate counts on plate count agar (PCA) at 0, 24, 48, 72 and 96 hour cultivation. Spectrophotometric observation showed that the highest bacterial growth of all tofu wastewater treatments indicated by the addition of 12% tofu wastewater, although the absorbance value was lower than culture in tryptic soy broth (TSB) as control. Exponential growth occurred between 0-24 hour incubation, and the highest growth indicated in substrate contained 12% tofu wastewater. Similarly, total plate count (TPC) analysis indicated that the highest bacterial growth of all treatment occurred at 24 hours incubation, and the highest count was also indicated by treatment of 12% tofu wastewater (2.42±0.06×108 CFU/mL). In conclusion, tofu wastewater can be an alternative substrate for the bacterial growth.

Pengaruh Suhu Penyimpanan terhadap Organoleptik, Derajat Keasaman dan Pertumbuhan Bakteri Coliform pada Susu Pasteurisasi

2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan
Keyword(s):  
Storage Temperature ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Initial Analysis ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Pathogenic Microbes ◽  
Total Coliform Bacteria

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi

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