Heat Treatment of Brussels Sprouts Retains Their Ability to Induce Detoxification Enzyme Expression In Vitro and In Vivo

2011 ◽  
Vol 76 (3) ◽  
pp. C454-C461 ◽  
Author(s):  
Melissa G. Robbins ◽  
Gaby Andersen ◽  
Veronika Somoza ◽  
Bruce D. Eshelman ◽  
David M. Barnes ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Enzyme Expression ◽  
Brussels Sprouts ◽  
Detoxification Enzyme

scholarly journals Attenuation of Carcinogenesis and the Mechanism Underlying by the Influence of Indole-3-carbinol and Its Metabolite 3,3′-Diindolylmethane: A Therapeutic Marvel

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
V. L. Maruthanila ◽  
J. Poornima ◽  
S. Mirunalini
Keyword(s):  
Epidemiological Studies ◽  
Cruciferous Vegetables ◽  
Brussels Sprouts ◽  
Anticancer Properties ◽  
Depth Study ◽  
Dimeric Product ◽  
Indole Glucosinolate

Rising evidence provides credible support towards the potential role of bioactive products derived from cruciferous vegetables such as broccoli, cauliflower, kale, cabbage, brussels sprouts, turnips, kohlrabi, bok choy, and radishes. Many epidemiological studies point out thatBrassicavegetable protects humans against cancer since they are rich sources of glucosinolates in addition to possessing a high content of flavonoids, vitamins, and mineral nutrients. Indole-3-carbinol (I3C) belongs to the class of compounds called indole glucosinolate, obtained from cruciferous vegetables, and is well-known for tits anticancer properties. In particular, I3C and its dimeric product, 3,3′-diindolylmethane (DIM), have been generally investigated for their value against a number of human cancersin vitroas well asin vivo. This paper reviews an in-depth study of the anticancer activity and the miscellaneous mechanisms underlying the anticarcinogenicity thereby broadening its therapeutic marvel.

scholarly journals Evaluation of Brassica Vegetables as Potential Feed for Ruminants

Animals ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 588 ◽  
Author(s):  
Trinidad de Evan ◽  
Andrea Vintimilla ◽  
Carlos N. Marcos ◽  
María José Ranilla ◽  
María Dolores Carro
Keyword(s):  
Neutral Detergent Fiber ◽  
Dry Matter Content ◽  
Ruminal Fermentation ◽  
Dairy Sheep ◽  
Brussels Sprouts ◽  
Brassica Vegetables ◽  
Intestinal Digestibility

The objective of this study was to analyze the chemical composition, in vitro ruminal fermentation, and intestinal digestibility of discarded samples of four Brassica vegetables: Brussels sprouts (BS), white cabbage, Savoy cabbage, and red cabbage, and to assess the effects of including increasing amounts of BS in the concentrate of a dairy sheep diet on in vitro fermentation, CH4 production, and in situ degradation of the diets. All cabbages had low dry matter content (DM; <16.5%), but their DM had high crude protein (19.5–24.8%) and sugars (27.2–41.4%) content and low neutral detergent fiber (17.5–28%) and was rapidly and extensively fermented in the rumen. Rumen degradability of protein at 12 h of in situ incubation was greater than 91.5% for all cabbages, and in vitro intestinal digestibility of protein ranged from 61.4 to 90.2%. Replacing barley, corn, and soybean meal by 24% of dried BS in the concentrate of a diet for dairy sheep (40:60 alfalfa hay:concentrate) increased in vitro diet fermentation and in situ degradability of DM and protein, and reduced in vitro CH4/total volatile fatty acid ratio. In vivo trials are necessary to confirm these results.

Therapy of Virus-Infected Plants by Heat Treatment I. Some Properties of Tomato Aspermy Virus and Its Inactivation at 36°C

1974 ◽  
Vol 22 (3) ◽  
pp. 437 ◽  
Author(s):  
GR Johnstone ◽  
GC Wade
Keyword(s):  
Heat Treatment ◽  
Order Reaction ◽  
Optimum Temperature ◽  
Tobacco Plants ◽  
Tomato Aspermy Virus ◽  
Induced Stress ◽  
Heat Treated ◽  
Enzyme Class

An isolate of tomato aspermy virus (TAV) was inactivated both in vivo and in vitro at 36°C. Inactivation took the form of a second or higher order reaction, which indicated that loss of infectivity was not due solely to a direct effect of high temperature on the virus. The concentration of polyphenoloxidases increased greatly in tobacco plants grown at 36°C, and evidence was obtained to indicate that this enzyme class, either directly or indirectly, enhanced the inactivation of TAV during heat treatment. The concentration of ribonucleases also increased in heat-treated tissues and these may have aided the inactivation, as the infectivity of TAV was shown to be destroyed by RNase in tests in vitro. The pH and ionic strength of the sap decreased in heated plants and these changes may have been significant as TAV had critical requirements of buffer pH and molarity for optimum infectivity. The alterations in cellular metabolism responsible for these changes result from heat-induced stress. Therefore, the optimum temperature for therapy of many viruses by heat treatment is likely to vary with the host in which it is treated, depending upon the host's heat tolerance.

scholarly journals 11β-Hydroxysteroid dehydrogenase and the pre-receptor regulation of corticosteroid hormone action

2005 ◽  
Vol 186 (2) ◽  
pp. 251-271 ◽  
Author(s):  
Nicole Draper ◽  
Paul M Stewart
Keyword(s):  
Molecular Basis ◽  
Hydroxysteroid Dehydrogenase ◽  
Hormone Action ◽  
Enzyme Expression ◽  
Human Obesity ◽  
Corticosteroid Hormone

Two isozymes of 11β-hydroxysteroid dehydrogenase (11β-HSD1 and 11β-HSD2) catalyse the interconversion of hormonally active cortisol and inactive cortisone. The enzyme evolved from a metabolic pathway to a novel mechanism underpinning human disease with the elucidation of the role of the type 2 or ‘kidney’ isozyme and an inherited form of hypertension, ‘apparent mineralocorti-coid excess’. ‘Cushing’s disease of the kidney’ arises because of a failure of 11β-HSD2 to inactivate cortisol to cortisone resulting in cortisol-induced mineralocorticoid excess. Conversely, 11β-HSD1 has been linked to human obesity and insulin resistance, but also to other diseases in which glucocorticoids have historically been implicated (osteoporosis, glaucoma). Here, the activation of cortisol from cortisone facilitates glucocorticoid hormone action at an autocrine level. The molecular basis for the putative human 11β-HSD1 ‘knockout’ – ‘cortisone reductase deficiency’ - has recently been described, an observation that also answers a long standing conundrum relating to the set-point of 11β-HSD1 activity. In each case, these clinical studies have been underpinned by studies in vitro and the manipulation of enzyme expression in vivo using recombinant mouse models.

A Study on the Interface between Oxide Film on the Titanium Surface and Bone Tissue

2007 ◽  
Vol 342-343 ◽  
pp. 545-548
Author(s):  
Li Ping Wang ◽  
Bang Cheng Yang ◽  
Ji Yong Chen ◽  
Xing Dong Zhang
Keyword(s):  
Heat Treatment ◽  
Oxide Film ◽  
Titanium Oxide ◽  
Titanium Surface ◽  
The Other ◽  
Apatite Formation ◽  
Titanium Oxide Film ◽  
Heat Treated

The bioactivities of titanium oxide film on titanium surface received from different chemical treatment methods were studied in SBF in vitro and mechanically and histologically investigated in vivo. Three groups of titanium specimens were prepared: untreated titanium(S), acid-alkali treated titanium (H), and acid-alkali and heat-treated titanium(X). The oxide film of X surface resulted in more apatite formation and significantly higher strength of the interface between the samples and bone than those of the other titanium groups. The surface of the acid-alkali treated titanium and that further treated by heat treatment had higher bioactivity and stronger bone-bonding ability.

scholarly journals Assessing the effects of severe heat treatment of milk on calcium bioavailability: In vitro and in vivo studies

2010 ◽  
Vol 93 (12) ◽  
pp. 5635-5643 ◽  
Author(s):  
I. Seiquer ◽  
C. Delgado-Andrade ◽  
A. Haro ◽  
M.P. Navarro
Keyword(s):  
Heat Treatment ◽  
In Vivo Studies ◽  
Calcium Bioavailability

scholarly journals The influence of storage and heat treatment on a magnesium-based implant material: an in vitro and in vivo study

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Katja Bracht ◽  
Nina Angrisani ◽  
Jan-Marten Seitz ◽  
Rainer Eifler ◽  
Andreas Weizbauer ◽  
...  
Keyword(s):  
Heat Treatment ◽  
In Vivo Study ◽  
Implant Material

scholarly journals Biomimetic Ceramic Composite: Characterization, Cell Response, and In Vivo Biocompatibility

Materials ◽  
2021 ◽  
Vol 14 (23) ◽  
pp. 7374
Author(s):  
Hung-Yang Lin ◽  
Yi-Jung Lu ◽  
Hsin-Hua Chou ◽  
Keng-Liang Ou ◽  
Bai-Hung Huang ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Cell Response ◽  
Treatment Time ◽  
Rabbit Model ◽  
In Vitro Cytotoxicity ◽  
Tissue Reaction ◽  
Mouse Fibroblast ◽  
Autogenous Bone

The present study aimed to synthesize biphasic calcium phosphate ceramics (CaPs) composed of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAp) from the propagated Scleractinian coral and dicalcium phosphate anhydrous using a solid-state reaction followed by heat treatment at a temperature of 1100 °C for 1 h to 7 days. The as-prepared coral and coral-derived biphasic CaPs samples were characterized through scanning electron microscopy, X-ray diffractometry, Fourier transform infrared spectroscopy, and Raman spectroscopy. The cell response of the biphasic CaPs was evaluated by in vitro cytotoxicity assessment using mouse fibroblast (L929) cells. The bilateral femoral defect rabbit model was used to assess the early local reaction of the coral-derived biphasic CaPs bone graft on tissue. The results confirmed that the co-existence of β-TCP and HAp was formed at 1100 °C for 1 h. The ratio of HA/β-TCP increased as the heat-treatment time increased. The coral-derived biphasic CaPs comprising 61% HAp and 39% β-TCP (defined as HT-3) were not cytotoxic. Furthermore, no significant differences in local tissue reaction were observed between the HT-3 sample and autogenous bone. Therefore, the synthesized coral-derived biphasic CaPs is a candidate for bone grafting due to its good biocompatibility.

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