Supercritical CO2 Decaffeination of Unroasted Coffee Beans Produces Melanoidins with Distinct NF-κB Inhibitory Activity

2011 ◽  
Vol 76 (7) ◽  
pp. H182-H186 ◽  
Author(s):  
Yumin Chen ◽  
Peter H. Brown ◽  
Kang Hu ◽  
Richard M. Black ◽  
Ronald L. Prior ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Supercritical Co2 ◽  
Coffee Beans

scholarly journals Study on α-glucosidase enzyme inhibitory activity and DPPH free radical scavenging of green coffee bean extract (Coffea canephora)

2019 ◽  
Vol 35 (2) ◽  
Author(s):  
Dang Kim Thu ◽  
Vu Manh Hung ◽  
Nguyen Thi Trang ◽  
Bui Thanh Tung
Keyword(s):  
Free Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging ◽  
Coffea Canephora ◽  
Coffee Bean ◽  
Green Coffee ◽  
Coffee Beans ◽  
Etoac Fraction ◽  
Treatment Of Diabetes ◽  
Green Coffee Bean Extract

Abstract: α-glucosidase enzyme is one of the important molecular targets in the treatment of diabetes. In addition, free radicals are the cause of insulin resistance, damage β- cell pancreatic, glucose uptake disorders and induced diabetes. In this study we evaluated the inhibitory effect of α-glucosidase enzyme and antioxidant effect by using DPPH free radical scavenging method of green coffee bean extract (Coffea canephora) and its fractions. Coffee beans were pulverized, extracted with ethanol 70% by sonications,  and fractionated with n-hexane, ethyl acetate (EtOAc) and n-butanol (n-BuOH) solvents. Our results showed that coffee bean extract has a strong α-glucosidase enzyme inhibitory activity, especially EtOAc fraction with an IC50 value of 2.21 ± 0.04 µg/mL. Furthermore, the coffee bean extract has an antioxidant effect by DPPH radical scavenging ability, and EtOAc fraction has the highest effect with an IC50 value of 25.69 ± 3.08 µg/ml. Our  results show that green coffee beans and EtOAc fraction have potential effect in preventing and supporting for the treatment of diabetes. Keywords Coffee; Coffea canephora; free radical; α-glucosidase; DPPH. References [1] Federation ID. IDF Diabetes Atlas 8th Edition (2017).[2] Wright Jr E, Scism‐Bacon J, Glass L. Oxidative stress in type 2 diabetes: the role of fasting and postprandial glycaemia. International journal of clinical practice 60(3) (2006) 308.[3] X. Chen. A review on coffee leaves: Phytochemicals, bioactivities and applications. Critical reviews in food science and nutrition 59(6) (2019) 1008.[4] Chu Y-F. Coffee: emerging health effects and disease prevention. John Wiley & Sons (2012).[5] N.T. Hai, D.K. Thu, B.T. Tung. Sarcandra glabra Extract Protects against Scopolamine Induced Cognitive Deficits by Modulating Neuroinflammation and the Cholinergic System. Current Enzyme Inhibition 14(3) (2018) 210.[6] B.T. Tung, D.K. Thu, P.T. Hai, N.T. Hai. Evaluation of α-glucosidase inhibitory activity of fractions from Punica granatum Linn fruits (in Vietnamese), Journal of Traditional Vietnamese Medicine and Pharmacy 5(18) (2018) 59.[7] S. Lenzen. The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia 51(2) (2008) 216.[8] K. Shapiro, W.C. Gong . Natural products used for diabetes. Journal of the American Pharmaceutical Association 42(2) (2002) 217.[9] O. Babova, A. Occhipinti, M.E. Maffei. Chemical partitioning and antioxidant capacity of green coffee (Coffea arabica and Coffea canephora) of different geographical origin. Phytochemistry 123 (2016) 33.[10] A. Priftis, D. Stagos, K. Konstantinopoulos, C. Tsitsimpikou, D.A. Spandidos, A.M. Tsatsakis, et al. Comparison of antioxidant activity between green and roasted coffee beans using molecular methods. Molecular medicine reports 12(5) (2015) 7293.[11] N. Liang, D.D. Kitts. Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules 19(11) (2014) 19180.[12] Vieira TMFdS. Potential antioxidant of brazilian coffee from the region of Cerrado. Food Science and Technology 38(3) (2018) 447.[13] S.D. Kim. α-Glucosidase inhibitor isolated from coffee. J Microbiol Biotechnol 25(2) (2015) 174.[14] Y. Zheng, K. Liu, G. Jia, H. Li, L. Han, Y. Kimura Effect of hot-water extract of coffee seeds on postprandial blood glucose concentration in rats. (2007).    

scholarly journals Metabolite Profiles of the Green Beans of Indonesian Arabica Coffee Varieties

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Nizar Happyana ◽  
Amelinda Pratiwi ◽  
Euis Holisotan Hakim
Keyword(s):  
1H Nmr ◽  
Inhibitory Activity ◽  
Quinic Acid ◽  
Green Beans ◽  
Arabica Coffee ◽  
Green Coffee Beans ◽  
Coffee Beans ◽  
Metabolite Profiles ◽  
Relationship Of ◽  
Alpha Glucosidase

The green beans of 3 Indonesian arabica coffee varieties, namely, ateng, buhun, and sigararutang, were analyzed with 1H NMR-based metabolomics coupled with alpha-glucosidase inhibitory activity assay. These coffees were cultivated in the same geographical conditions. The PLSDA model successfully classified the green coffee beans based on their varieties. To reveal the characteristic metabolites for each coffee variety, S-plot of two-class OPLSDA models was generated and analyzed. Ateng coffee was characterized with trigonelline, sucrose, 5-CQA, and acetic acid. The characteristic metabolites of buhun coffee were citric acid and malic acid. Meanwhile, the most discriminant compound of sigararutang coffee was quinic acid. HCA analysis revealed the lineage relationship of the 3 coffee varieties. Ateng coffee had closer lineage relationship to sigararutang compared to the buhun coffee. Alpha-glucosidase inhibitory activity of the coffee samples did not differ widely. I C 50 values of alpha-glucosidase inhibitory activity of ateng, sigararutang, and buhun coffees were 3.01 ± 0.16 , 3.14 ± 0.20 , and 5.05 ± 0.28  mg/mL, respectively. Although grown in the same geographical conditions, our results revealed that each coffee variety possessed a unique metabolome clarifying the diversity of Indonesian arabica coffees. This study verified that 1H NMR-based metabolomics is an excellence method for discovering the lineage relationship in the samples with different varieties or cultivars.

scholarly journals COX-2 Inhibitory Activity of Cafestol and Analogs from Coffee Beans

2008 ◽  
Vol 3 (1) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Ilias Muhammad ◽  
Satoshi Takamatsu ◽  
Jamal Mustafa ◽  
Shabana I. Khan ◽  
Ikhlas A. Khan ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Inhibitory Activity ◽  
Coffea Arabica ◽  
Structure Elucidation ◽  
Biological Activities ◽  
Cell Aggregation ◽  
Gel Chromatography ◽  
Coffee Beans ◽  
Cox 2 ◽  
Silica Gel Chromatography

Two kaurane diterpenes, namely cafestol (1) and kahweol (2), were isolated from hydrolyzed fraction of the fixed oil of Ethiopian Coffea arabica unroasted beans, using AgNO3-impregnated silica gel chromatography. In addition, cafestol palmitate (3) and kahweol palmitate (4), the two natural diterpene esters of C. arabica, were also synthesized. Compounds 1–4 were evaluated for anti-inflammatory activity using cyclooxygenase-2 (COX-2), cell aggregation, cell proliferation, cell adhesion, iNOS and antioxidant assays. The COX-2 inhibitory activity of cafestol (1) was found to be 20-fold more potent than kahweol (2) (IC50 value 0.25 μg/mL vs. 5.0 μg/mL), while compounds 3 and 4 were weakly active. The isolation and structure elucidation of the diterpenes 1 and 2, preparation of compounds 3 and 4, and their biological activities are presented in this paper.

Supercritical CO2-ethanol extraction of oil from green coffee beans: optimization conditions and bioactive compound identification

2021 ◽  
Author(s):  
Paloma Barajas-Álvarez ◽  
Gustavo A. Castillo-Herrera ◽  
Guadalupe M. Guatemala-Morales ◽  
Rosa I. Corona-González ◽  
Enrique Arriola-Guevara ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Bioactive Compound ◽  
Green Coffee ◽  
Compound Identification ◽  
Ethanol Extraction ◽  
Green Coffee Beans ◽  
Coffee Beans

scholarly journals Decaffeination and Neuraminidase Inhibitory Activity of Arabica Green Coffee (Coffea arabica) Beans: Chlorogenic Acid as a Potential Bioactive Compound

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3402
Author(s):  
Muchtaridi Muchtaridi ◽  
Dwintha Lestari ◽  
Nur Kusaira Khairul Ikram ◽  
Amirah Mohd Gazzali ◽  
Maywan Hariono ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Inhibitory Activity ◽  
Solid Phase ◽  
Bioactive Compound ◽  
Pharmacophore Modeling ◽  
Coffee Bean ◽  
Binding Interaction ◽  
Coffee Beans ◽  
Before And After

Coffee has been studied for its health benefits, including prevention of several chronic diseases, such as type 2 diabetes mellitus, cancer, Parkinson’s, and liver diseases. Chlorogenic acid (CGA), an important component in coffee beans, was shown to possess antiviral activity against viruses. However, the presence of caffeine in coffee beans may also cause insomnia and stomach irritation, and increase heart rate and respiration rate. These unwanted effects may be reduced by decaffeination of green bean Arabica coffee (GBAC) by treatment with dichloromethane, followed by solid-phase extraction using methanol. In this study, the caffeine and chlorogenic acid (CGA) level in the coffee bean from three different areas in West Java, before and after decaffeination, was determined and validated using HPLC. The results showed that the levels of caffeine were reduced significantly, with an order as follows: Tasikmalaya (2.28% to 0.097% (97 ppm), Pangalengan (1.57% to 0.049% (495 ppm), and Garut (1.45% to 0.00002% (0.2 ppm). The CGA levels in the GBAC were also reduced as follows: Tasikmalaya (0.54% to 0.001% (118 ppm), Pangalengan (0.97% to 0.0047% (388 ppm)), and Garut (0.81% to 0.029% (282 ppm). The decaffeinated samples were then subjected to the H5N1 neuraminidase (NA) binding assay to determine its bioactivity as an anti-influenza agent. The results show that samples from Tasikmalaya, Pangalengan, and Garut possess NA inhibitory activity with IC50 of 69.70, 75.23, and 55.74 μg/mL, respectively. The low level of caffeine with a higher level of CGA correlates with their higher levels of NA inhibitory, as shown in the Garut samples. Therefore, the level of caffeine and CGA influenced the level of NA inhibitory activity. This is supported by the validation of CGA-NA binding interaction via molecular docking and pharmacophore modeling; hence, CGA could potentially serve as a bioactive compound for neuraminidase activity in GBAC.

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