Comparative Evaluation of Antioxidant Reactivity between Ovariectomized and Control Urinary Bladder Tissue Using Ferric Reducing Ability of Plasma and Cupric Ion Reducing Antioxidant Capacity Assays

The aim of the present study was to assess whether probiotic bacteriaLactobacillus casei(4 × 108 CFU) influences the antioxidant properties of human plasma when combined with prebiotic Inulin (400 mg). Experiments were carried out on healthy volunteers (n=32). Volunteers were divided according to sex (16 male and 16 female) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 weeks, at the end of the study. Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activity, and the ferric reducing ability of plasma (FRAP) in human plasma were examined. The administration of synbiotics containingL. caseiplus Inulin resulted in a significant increase in FRAP values (p=0.00008) and CAT activity (p=0.02) and an insignificant increase in SOD and GPx activity compared to controls. Synbiotics containingL. casei(4 × 108 CFU) with prebiotic Inulin (400 mg) may have a positive influence on human plasma antioxidant capacity and the activity of selected antioxidant enzymes.

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Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay

Microchimica Acta ◽

10.1007/s00604-007-0777-0 ◽

2007 ◽

Vol 160 (4) ◽

pp. 413-419 ◽

Cited By ~ 243

Author(s):

Reşat Apak ◽

Kubilay Güçlü ◽

Mustafa Özyürek ◽

Saliha Esin Çelik

Keyword(s):

Antioxidant Capacity ◽

Cupric Ion ◽

Antioxidant Capacity Assays

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Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

Molecules ◽

10.3390/12071496 ◽

2007 ◽

Vol 12 (7) ◽

pp. 1496-1547 ◽

Cited By ~ 444

Author(s):

Reşat Apak ◽

Kubilay Güçlü ◽

Birsen Demirata ◽

Mustafa Özyürek ◽

Saliha Çelik ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Comparative Evaluation ◽

Total Antioxidant ◽

Antioxidant Capacity Assays

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Comparative evaluation of Fe(III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents

Talanta ◽

10.1016/j.talanta.2007.01.019 ◽

2007 ◽

Vol 72 (3) ◽

pp. 1157-1165 ◽

Cited By ~ 131

Author(s):

Kadriye Işıl Berker ◽

Kubilay Güçlü ◽

İzzet Tor ◽

Reşat Apak

Keyword(s):

Antioxidant Capacity ◽

Comparative Evaluation ◽

Reducing Power ◽

Antioxidant Capacity Assays

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Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000654 ◽

2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Masoud Nasiri ◽

Saja Ahmadizad ◽

Mehdi Hedayati ◽

Tayebe Zarekar ◽

Mehdi Seydyousefi ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Wistar Rats ◽

Enzymatic Antioxidants ◽

Acute Response ◽

Total Antioxidant ◽

Male Wistar Rats ◽

Exercise Induced ◽

And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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A Prompt, Tough and Eco-Friendly (PTOCO) System for Mini-Scale Extraction of Samples for Antioxidant Capacity Assays

Journal of the Brazilian Chemical Society ◽

10.5935/0103-5053.20150299 ◽

2015 ◽

Author(s):

Thiago Claus ◽

Oscar O. Santos Júnior ◽

Janksyn Bertozzi ◽

Swami A. Maruyama ◽

Sylvio V. Palombini ◽

...

Keyword(s):

Antioxidant Capacity ◽

Antioxidant Capacity Assays

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Lower antioxidant capacity in the prefrontal cortex of individuals with schizophrenia

Australian & New Zealand Journal of Psychiatry ◽

10.1177/0004867417728805 ◽

2017 ◽

Vol 52 (7) ◽

pp. 690-698 ◽

Cited By ~ 8

Author(s):

Yiru Zhang ◽

Vibeke Sørensen Catts ◽

Cynthia Shannon Weickert

Keyword(s):

Prefrontal Cortex ◽

Antioxidant Capacity ◽

Mrna Expression ◽

Antioxidant System ◽

Messenger Rna ◽

Total Glutathione ◽

Protein Levels ◽

Significant Difference ◽

Dorsolateral Prefrontal ◽

And Control

Objective: The glutathione (GSH) pathway is the main antioxidant system to protect against oxidative stress in the human brain. In this study, we tested whether molecular components of the GSH antioxidant system are changed in dorsolateral prefrontal cortex tissue from people with schizophrenia compared to controls. Method: The levels of total glutathione and reduced GSH were determined by fluorometric assay via quantifying thiols in extracts from frontal cortex of 68 people. Immunoblotting was used to measure levels of enzymes responsible for maintaining GSH, the glutamyl-cysteine ligase (GCL) catalytic subunit (GCLC) and the GSH peroxidase (GPx)-like protein ( n = 74). Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to measure GCLC messenger RNA (mRNA) expression. Results: Both total glutathione ( t(66) = 2.467, p = 0.016) and reduced GSH ( t(66) = 3.001, p = 0.004) levels were significantly less in people with schizophrenia than in controls. However, there were no significant differences in either GCLC-like protein ( t(72) = −1.077, p = 0.285) or GCLC mRNA expression ( t(71) = −0.376, p = 0.708) between people with schizophrenia and control subjects. There was also no significant difference of GPx-like protein levels between schizophrenia and controls ( t(72) = −0.060, p = 0.952). Moreover, no significant correlations of putative confounding factors with GSH changes were detected. Discussion: These results suggest that people with schizophrenia have impaired GSH antioxidant capacity, alongside normal levels of key regulatory proteins.

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Salivary Antioxidant Status in Patients with Oral Lichen Planus: Correlation with Clinical Signs and Evolution during Treatment withChamaemelum nobile

BioMed Research International ◽

10.1155/2018/5187549 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

Asta Tvarijonaviciute ◽

Cristina Aznar-Cayuela ◽

Camila P. Rubio ◽

Fernando Tecles ◽

Jose J. Ceron ◽

...

Keyword(s):

Antioxidant Capacity ◽

Lichen Planus ◽

Antioxidant Status ◽

Oral Lichen Planus ◽

Clinical Signs ◽

Total Antioxidant Status ◽

Double Blind ◽

Reducing Ability ◽

Chamaemelum Nobile ◽

Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disease, which manifests as a succession of outbreaks. OLP was associated with salivary oxidative stress. Randomized, double blind, parallel-group study was performed. The sample consisted of 55 clinically and histopathologically diagnosed OLP patients. Twenty-six patients were treated with 2%Chamaemelum nobilegel and 29 with a placebo. Nonstimulated (basal) saliva was collected on the first day of the study and 4 weeks later. Salivary total antioxidant status (TAS) was evaluated by four different methods: two TAC (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equivalent antioxidant capacity methods (TAC1 and TAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). At baseline (T1), no statistically significant differences were detected in any of the TAS analytes between the two groups of patients. After four weeks of treatment, a statistically significant increase was detected in FRAP in the placebo group (0.323 [0.090–0.467] versus 0.406 [0.197–0.848] mmol/g⁎10-3) (P<0.05). Significant correlations were observed between pain and drainage and TAC1, CUPRAC, and FRAP and between xerostomia and the TAC1, TAC2, CUPRAC, and FRAP. The results of the present study showed that in patients with OLP increases of TAS in saliva are associated with increase in pain and xerostomia and decrease in drainage, suggesting a worsening condition of the patient. The use ofChamaemelum nobilegel would be recommended for disease stabilization.

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