Ablation of ceramide synthase 2 exacerbates dextran sodium sulphate-induced colitis in mice due to increased intestinal permeability

This study evaluated the effects of Bifidobacterium longum 51A on the intestinal mucosa and inflammatory response in experimental colitis. Colitis was induced by administration of 3.5% dextran sodium sulphate (DSS) solution for 7 days. Two periods of administration were performed: treatment (T) group, mice received Bifidobacterium only during disease induction (7 days); total treatment (TT) group, mice received Bifidobacterium for 10 days before and during disease induction. The probiotic effects on intestinal permeability, inflammatory infiltrate, histological analysis, cytokines, chemokines and sIgA were evaluated. Bifidobacterium administration in the T group showed reduction in intestinal permeability and lower IL-1β, myeloperoxidase, and eosinophil peroxidase levels compared to those in the colitis group (P<0.05). Bifidobacterium administration in the TT group attenuated severe lesions in the colon and reduced eosinophil peroxidase level (P<0.05). B. longum 51A treatment modality was more effective than total treatment and reduced the inflammatory response and its consequences on intestinal epithelium.

Download Full-text

Faculty Opinions recommendation of Helicobacter pylori DNA decreases pro-inflammatory cytokine production by dendritic cells and attenuates dextran sodium sulphate-induced colitis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.10005956.14859111 ◽

2012 ◽

Author(s):

Steven Moss ◽

Songhua Zhang

Keyword(s):

Helicobacter Pylori ◽

Dendritic Cells ◽

Inflammatory Cytokine ◽

Cytokine Production ◽

Sodium Sulphate ◽

Dextran Sodium Sulphate ◽

Inflammatory Cytokine Production

Download Full-text

Titanium Dioxide Presents a Different Profile in Dextran Sodium Sulphate-Induced Experimental Colitis in Mice Lacking the IBD Risk Gene Ptpn2 in Myeloid Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22020772 ◽

2021 ◽

Vol 22 (2) ◽

pp. 772

Author(s):

Javier Conde ◽

Marlene Schwarzfischer ◽

Egle Katkeviciute ◽

Janine Häfliger ◽

Anna Niechcial ◽

...

Keyword(s):

Bone Marrow ◽

Titanium Dioxide ◽

Genetic Risk ◽

Intestinal Inflammation ◽

Sodium Sulphate ◽

Food Additive ◽

Wild Type ◽

Dextran Sodium Sulphate ◽

Risk Gene ◽

The Impact

Environmental and genetic factors have been demonstrated to contribute to the development of inflammatory bowel disease (IBD). Recent studies suggested that the food additive; titanium dioxide (TiO2) might play a causative role in the disease. Therefore, in the present study we aimed to explore the interaction between the food additive TiO2 and the well-characterized IBD risk gene protein tyrosine phosphatase non-receptor type 2 (Ptpn2) and their role in the development of intestinal inflammation. Dextran sodium sulphate (DSS)-induced acute colitis was performed in mice lacking the expression of Ptpn2 in myeloid cells (Ptpn2LysMCre) or their wild type littermates (Ptpn2fl/fl) and exposed to the microparticle TiO2. The impact of Ptpn2 on TiO2 signalling pathways and TiO2-induced IL-1β and IL-10 levels were studied using bone marrow-derived macrophages (BMDMs). Ptpn2LysMCre exposed to TiO2 exhibited more severe intestinal inflammation than their wild type counterparts. This effect was likely due to the impact of TiO2 on the differentiation of intestinal macrophages, suppressing the number of anti-inflammatory macrophages in Ptpn2 deficient mice. Moreover, we also found that TiO2 was able to induce the secretion of IL-1β via mitogen-activated proteins kinases (MAPKs) and to repress the expression of IL-10 in bone marrow-derived macrophages via MAPK-independent pathways. This is the first evidence of the cooperation between the genetic risk factor Ptpn2 and the environmental factor TiO2 in the regulation of intestinal inflammation. The results presented here suggest that the ingestion of certain industrial compounds should be taken into account, especially in individuals with increased genetic risk

Download Full-text

Orally administered RDP58 reduces the severity of dextran sodium sulphate induced colitis

Annals of the Rheumatic Diseases ◽

10.1136/ard.61.suppl_2.ii19 ◽

2002 ◽

Vol 61 (Supplement 2) ◽

pp. 19ii-24 ◽

Cited By ~ 19

Author(s):

R Boismenu ◽

Y Chen ◽

K Chou ◽

A El-Sheikh ◽

R Buelow

Keyword(s):

Sodium Sulphate ◽

Dextran Sodium Sulphate

Download Full-text

Involvement of CPI-17 downregulation in the dysmotility of the colon from dextran sodium sulphate-induced experimental colitis in a mouse model

Neurogastroenterology & Motility ◽

10.1111/j.1365-2982.2007.00911.x ◽

2007 ◽

Vol 19 (6) ◽

pp. 504-514 ◽

Cited By ~ 31

Author(s):

k. sato ◽

s. ohkura ◽

y. kitahara ◽

t. ohama ◽

m. hori ◽

...

Keyword(s):

Mouse Model ◽

Experimental Colitis ◽

Sodium Sulphate ◽

Dextran Sodium Sulphate

Download Full-text

Inhibitory Activity Goblet Depletion and focal inflammatory Phaleria macrocarpha Leaves Ethanol Extract on Crypta Mouse after Dextran Sodium Sulphate Induction

International Journal of PharmTech Research ◽

10.20902/ijptr.2019.120106 ◽

2019 ◽

Vol 12 (01) ◽

pp. 37-48

Author(s):

Rianti Maharani ◽

Kusmardi ◽

Berna Elya

Keyword(s):

Inhibitory Activity ◽

Ethanol Extract ◽

Sodium Sulphate ◽

Dextran Sodium Sulphate

Download Full-text

POTENTIAL OF ETHANOL EXTRACT OF MAHKOTA DEWA LEAVES (PHALERIA MACROCARPA (SCHECFF.) BOERL.) TO INHIBIT INFLAMMATION IN MOUSE DISTAL COLON INDUCED BY DEXTRAN SODIUM SULFATE (DSS) AND AZOXYMETHANE (AOM)

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s3.39490 ◽

2020 ◽

pp. 101-105

Author(s):

MUHAMMAD ILHAM DHIYA RAKASIWI ◽

KUSMARDI KUSMARDI ◽

ARI ESTUNINGTYAS ◽

ARYO TEDJO

Keyword(s):

Leaf Extract ◽

Histopathological Examination ◽

Ethanol Extract ◽

Distal Colon ◽

Goblet Cells ◽

Sodium Sulphate ◽

Dextran Sodium Sulphate ◽

Phaleria Macrocarpa ◽

Hematoxylin Eosin

Objective: To demonstrates the ability of P. macrocarpa leaf extract to reduce inflammation of the distal colon in DSS/AOM-induced mice. Methods: In vivo experimental research using Balb/c mice induced by 0.2 ml azoxymethane (AOM) 0.1% once and 1% dextran sodium sulphate (DSS) for one week; additionally, ethanol extract of P. macrocarpa leaves, 25 mg and 50 mg, and 0.84 mg acetosal were given orally. The mice were sacrificed after 20 w. Histopathological examination (hematoxylin-eosin staining) was conducted by counting the average number of goblet cells per crypt, inflammatory focus and angiogenesis. Results: Ethanol extract of P. macrocarpa leaves was able to prevent the decrease in the number of goblet cells (p<0.05). However, the administration of ethanol P. macrocarpa leaf extract could not reduce focal inflammation and angiogenesis in inflammation of the distal colon. Conclusion: Ethanol extract of the Mahkota Dewa leaves is able to prevent inflammation of the distal colon by preventing the decrease in the number of goblet cells.

Download Full-text