Antimicrobial sensitivity of pathogenic
            Yersinia ruckeri
            from central and southern highlands of Peru

General objectives: This study aimed at assessment of factors affecting antimicrobial sensitivity in Staphylococcus aureus clinical isolates from Assir region, Saudi Arabia. Materials and Methods: In this study, eighty one patients presented with Staph. aureus infections either nosocomial or community acquired infections were involved by collecting nasal swabs from them at Aseer Central Hospital General Lab. These patients were from all age groups and from males and females during the period of Jan 2011- Jun 2011. These samples were undergone variable laboratory procedures mainly; bactech, culture media, antibiotics sensitivity test using diffusion disc test (MIC) and molecular (PCR) for detection of mec A gene. Clinical and laboratory data were recorded in special formats and analyzed by statistical computer program (SPSS). Results: Showed that; Descriptive and analytical statistical analysis were performed and final results were plotted in tables. In Staph aureus MecA gene positive cases (50) showed: Oxacillin/ Mithicillin, Ciprofloxacin and Fusidin resistant in diabetic patients were 13, 26.0%, 9, 18% and 7, 14% respectively and in non diabetic patients were 37, 74.0%, 22, 44% and 20, 40% respectively. While no sensitivity in diabetic and non diabetic patients using Oxacillin/ Mithicillin. In Staph aureus MecA gene negative cases (31) showed: Oxacillin/ Mithicillin, sensitivity in diabetic patients (5, 16.1%) and in non diabetic were (26, 83.9%). While no resistant in diabetic and non diabetic patients. In Ciprofloxacin and Fusidin resistant in diabetic patients were 1, 3.2% and 1, 3.2% respectively and in non diabetic patients were 12, 38.7% and 7, 22.6%respectively. Erythromycin in Staph aureus ( MecA gene) positive cases (50) showed: resistant in age (0-15) years were (5, 10%), (16-50) years were (16, 32%) and ( 50 years) were (12, 24%). Erythromycin in Staph aureus (MecA gene) negative cases (31) showed: resistant in age (0-15) years were (6, 19.3%), (16-50) years were (5, 16.1%) and ( 50 years) were (3, 9.7%). Conclusion: Drugs resistance is a major progressive multifactorial problem facing the treatment of Staph aureus infections. DOI: http://dx.doi.org/10.3329/jom.v13i2.12750 J Medicine 2012; 13 : 152-159

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Exemplar Abstract for Yersinia ruckeri Ewing et al. 1978 (Approved Lists 1980).

The NamesforLife Abstracts ◽

10.1601/ex.3366 ◽

2003 ◽

Author(s):

Charles Thomas Parker ◽

Kara Mannor ◽

George M Garrity

Keyword(s):

Yersinia Ruckeri

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A further Characterization of Yersinia ruckeri (Enteric Redmouth Bacterium)

Fish Pathology ◽

10.3147/jsfp.14.71 ◽

1979 ◽

Vol 14 (2) ◽

pp. 71-78 ◽

Cited By ~ 11

Author(s):

P.J. O'LEARY ◽

J.S. ROHOVEC ◽

J.L. FRYER

Keyword(s):

Yersinia Ruckeri

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STUDY AND CHARACTERIZATION GROWTH OF FOUR MICROALGAE SPECIES AND TEST ANTIMICROBIAL ACTIVITY

Jurnal Zarah ◽

10.31629/zarah.v6i2.625 ◽

2018 ◽

Vol 6 (2) ◽

pp. 53-58

Author(s):

Marniati Salim

Keyword(s):

Dunaliella Salina ◽

Methanol Extract ◽

Sea Water ◽

Basal Medium ◽

Nannochloropsis Oculata ◽

Inhibitory Zone ◽

Antimicrobial Sensitivity ◽

Sensitivity Tests ◽

Microalgae Biomass ◽

Chaetoceros Calcitrans

Abstract In this study to the growth characteristics of microalgae (Dunaliella salina, Nannochloropsis oculata, Tetrasel mis chuii & Chaetoceros calcitrans), in different mediums, namely Bold’s Basal Medium (BBM) and BBM modification of sea water. The results obtained from microalgae (N.oculata, T.chuii, C.calcitrans) are better grown on BBM media while microlaga D.salina grows well on BBM modification of sea water. Microalgae biomass is extracted by maceration method in hexane and methanol solvents. Test bacteria used gram positive (Staphylococcus aureus) and gram negative (Escherichia coli). Inhibitory zone diameter results were compared with antimicrobial sensitivity tests according to Clinical and Laboratory Standards Institute (CLSI). From the results of microalgae hexane and methanol extract antibacterial activity tests (D.salina, N.oculata, T.chuii, C.calcitrans) showed that the extract was not too sensitive to inhibit bacterial growth. Keywords: microalgae, medium, antimicrobial

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Microarray Technology for the Detection of Antimicrobial Resistance Genes Present in Hospital and Community Acquired Methicillin Resistant S.aureus Strains

Revista de Chimie ◽

10.37358/rc.17.11.5925 ◽

2017 ◽

Vol 68 (11) ◽

pp. 2546-2550

Author(s):

Monica Licker ◽

Andrei Anghel ◽

Roxana Moldovan ◽

Elena Hogea ◽

Delia Muntean ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Dna Microarray ◽

Bacterial Resistance ◽

Meca Gene ◽

Modern Medicine ◽

Microarray Technology ◽

Methicillin Resistant ◽

Antimicrobial Sensitivity ◽

Antimicrobial Resistance Genes ◽

Mrsa Strains

Antimicrobial resistance (AMR) represents a real burden for the modern medicine. One of the most frecvently isolated hospital acquired (HA) pathogens wordlwide, is Methicillin resistant Staphylococcus aureus (MRSA). Recently not only HA, but also community-acquired MRSA (CA-MRSA) infections have been reported. A prospective study was performed between February 2009 and October 2010, with the aim to investigate bacterial resistance of CA-MRSA and HA-MRSA. DNA microarray technology has been used for the detection of 4 AMR genes for the studied MRSA strains. A number of 218 HA- S.aureus strains have been isolated, from which 89 (40. 82%) were MRSA. In the community, 1.553 S.aureus strains were isolated, out of which, 356 (22. 92%) were MRSA. From these, a number of 17 HA and 12 CA �MRSA strains have been analyzed by DNA microarray technology. From 100% phenotypically described HA- MRSA, we identified mecA gene in 10 strains (58. 83%). Other 6 strains (35. 29%) have been erm(A) positive and 4 (23. 53%) - tet(O) positive. 83. 33% (10 strains) from the CA strains had mecA gene, only one (8. 33%) was erm(A) positive and 4 (33. 33%) were erm(C) positive. DNA microarray is a method allowing the concomitant scan of multiple genes and can be done within a few hours. That type of rapid and reliable methods for antimicrobial sensitivity tests are important to start an appropriate therapy.

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Antibiotic Molecules Involved in Increasing Microbial Resistance

Revista de Chimie ◽

10.37358/rc.19.7.7392 ◽

2019 ◽

Vol 70 (7) ◽

pp. 2622-2626 ◽

Cited By ~ 5

Author(s):

Letitia Doina Duceac ◽

Constantin Marcu ◽

Daniela Luminita Ichim ◽

Irina Mihaela Ciomaga ◽

Elena Tarca ◽

...

Keyword(s):

Intensive Care ◽

Intensive Care Units ◽

Enterobacter Aerogenes ◽

Healthcare Associated Infections ◽

Medical Department ◽

Microbial Resistance ◽

Antimicrobial Sensitivity ◽

Interpretation Criteria ◽

Tract Infections ◽

Healthcare Associated

Over the past two decades, the resistance to antibiotics, especially for Gram-negative bacteria, has increased at an alarming rate, requiring constant concern for resolving and controlling this extremely important therapeutic aspect in any medical department but in particular, in Anaesthesia and Intensive Care Units, in units of neonatology, paediatrics, neurosurgery, burned patients and immunosuppressed. Specialists note a particular concern for the resistance of Enterobacteriaceae to third-generation cephalosporins and aztreonam, with a resistance profile frequently associated with the expression of extended-spectrum �-lactamases (ESBL). The Enterobacter genus comprises 14 species, but two are of medical interest, Enterobacter aerogenes and E. cloacae, which are involved in inducing healthcare-associated infections such as urinary tract infections, pneumonia associated with mechanical ventilation, bacteremia, septicemia, etc. The purpose of the study was to highlight the antibiotic molecules in which microbial resistance of some circulating strains of enterobacteria was detected. A descriptive and retrospective study was conducted between 2012-2017, on a batch of 35 patients, admitted to the Sf. Maria Emergency Clinical Hospital for Children of Iasi, from whom various pathological products were collected to highlight the Enterobacter sp strains involved in the production of infections associated with the inpatient medical care. The antimicrobial sensitivity of each strain was determined by diffusimetric method, while the interpretation criteria were considered to be those of the laboratory standards. Most cases were reported in 2017 (31.42%). The majority were registered in new-borns (42.85%) and infants (25.71%). The Anaesthesia and Intensive Care Units and Neonatology Anaesthesia and Intensive Care Units departments were the most involved. Microbial antibacterial resistance of Enterobacter sp isolates showed that all manifested resistance to ampicillin, amoxicillin and clavulanic acid, 48.57% were resistant to Cefuroxime, 42.85% resistant to Ceftazidime and Ceftriaxone, 14.28% to ciprofloxacin, 11.42% to ertapenem, 5.71% to Meronem. Although it showed relatively few cases with infections associated with healthcare in which strains of Enterobacter sp. were isolated our study, which was carried out over a period of 5 years, provides useful indications regarding the prevalence of healthcare associated infections with Enterobacter sp in paediatric patients and guidelines for antibiotic therapy.

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