PEG‐modified subunit vaccine encoding dominant epitope to enhance immune response against spring viraemia of carp virus

2021 ◽  
Author(s):  
Hui‐Xin Duan ◽  
Zhao Zhao ◽  
Ying‐Jie Jin ◽  
Zi‐Long Wang ◽  
Jie‐Fang Deng ◽  
...  
Keyword(s):  
Immune Response ◽  
Subunit Vaccine ◽  
Dominant Epitope

scholarly journals Maternally Derived Antibody Levels Influence on Vaccine Protection against PCV2d Challenge

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2231
Author(s):  
István Kiss ◽  
Krisztina Szigeti ◽  
Zalán G. Homonnay ◽  
Vivien Tamás ◽  
Han Smits ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Subunit Vaccine ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Vaccine Protection ◽  
Humoral Immune ◽  
Negative Effect ◽  
Antibody Levels

Piglets from a porcine circovirus type 2 (PCV2) stable farm of low and high levels of maternally derived antibodies (MDA) against PCV2 were vaccinated either with a whole virus type or a PCV2 ORF2 antigen-based commercial subunit vaccine at three weeks of age. Two non-vaccinated groups served as low and high MDA positive controls. At four weeks post vaccination, all piglets were challenged with a PCV2d-2 type virus strain and were checked for parameters related to vaccine protection over a four-week observation period. MDA levels evidently impacted the outcome of the PCV2d-2 challenge in non-vaccinated animals, while it did not have a significant effect on vaccine-induced protection levels. The humoral immune response developed faster in the whole virus vaccinates than in the subunit vaccinated pigs in the low MDA groups. Further, high MDA levels elicited a stronger negative effect on the vaccine-induced humoral immune response for the subunit vaccine than for the whole virus vaccine. The group-based oral fluid samples and the group mean viraemia and faecal shedding data correlated well, enabling this simple, and animal welfare-friendly sampling method for the evaluation of the PCV2 viral load status of these nursery piglets.

scholarly journals Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin

2012 ◽  
Vol 19 (5) ◽  
pp. 740-745 ◽  
Author(s):  
André A. Grassmann ◽  
Samuel R. Félix ◽  
Carolina Ximendes dos Santos ◽  
Marta G. Amaral ◽  
Amilton C. P. Seixas Neto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Subunit Vaccine ◽  
Lethal Dose ◽  
Control Group ◽  
Hamster Model ◽  
Content Type ◽  
B Subunit ◽  
Heat Labile ◽  
A Subunit

ABSTRACTLeptospirosis, a worldwide zoonosis, lacks an effective, safe, and cross-protective vaccine. LipL32, the most abundant, immunogenic, and conserved surface lipoprotein present in all pathogenic species ofLeptospira, is a promising antigen candidate for a recombinant vaccine. However, several studies have reported a lack of protection when this protein is used as a subunit vaccine. In an attempt to enhance the immune response, we used LipL32 coupled to or coadministered with the B subunit of theEscherichia coliheat-labile enterotoxin (LTB) in a hamster model of leptospirosis. After homologous challenge with 5× the 50% lethal dose (LD50) ofLeptospira interrogans, animals vaccinated with LipL32 coadministered with LTB and LTB::LipL32 had significantly higher survival rates (P< 0.05) than animals from the control group. This is the first report of a protective immune response afforded by a subunit vaccine using LipL32 and represents an important contribution toward the development of improved leptospirosis vaccines.

scholarly journals Protection of Mice with a Tuberculosis Subunit Vaccine Based on a Fusion Protein of Antigen 85B and ESAT-6

2001 ◽  
Vol 69 (5) ◽  
pp. 2773-2778 ◽  
Author(s):  
Anja Weinreich Olsen ◽  
Laurens A. H. van Pinxteren ◽  
Limei Meng Okkels ◽  
Peter Birk Rasmussen ◽  
Peter Andersen
Keyword(s):  
Immune Response ◽  
Fusion Proteins ◽  
Protective Immunity ◽  
Subunit Vaccine ◽  
Lipid A ◽  
Dose Range ◽  
Monophosphoryl Lipid A ◽  
Antigen 85B ◽  
Immunodominant Antigens ◽  
Dose Dependent

ABSTRACT In this study, we investigated the potential of a tuberculosis subunit vaccine based on fusion proteins of the immunodominant antigens ESAT-6 and antigen 85B. When the fusion proteins were administered to mice in the adjuvant combination dimethyl dioctadecylammonium bromide-monophosphoryl lipid A, a strong dose-dependent immune response was induced to both single components as well as to the fusion proteins. The immune response induced was accompanied by high levels of protective immunity and reached the level of Mycobacterium bovis BCG-induced protection over a broad dose range. The vaccine induced efficient immunological memory, which remained stable 30 weeks postvaccination.

Development a multi-epitope driven subunit vaccine for immune response reinforcement against Serogroup B of Neisseria meningitidis using comprehensive immunoinformatics approaches

2019 ◽  
Vol 75 ◽  
pp. 103992
Author(s):  
Maryam Rostamtabar ◽  
Abolfazl Rahmani ◽  
Masoud Baee ◽  
Ahmad Karkhah ◽  
Vijay Kumar Prajapati ◽  
...  
Keyword(s):  
Immune Response ◽  
Neisseria Meningitidis ◽  
Subunit Vaccine

One-Pot Generating Subunit Vaccine with High Encapsulating Efficiency and Fast Lysosome Escape for Potent Cellular Immune Response

2020 ◽  
Vol 31 (8) ◽  
pp. 1917-1927
Author(s):  
Pengyan Hao ◽  
Xiaoxiao Wu ◽  
Liwen Wang ◽  
Shaoyin Wei ◽  
Hongyan Xu ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Subunit Vaccine ◽  
One Pot ◽  
Cellular Immune

scholarly journals A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

2013 ◽  
Vol 20 (5) ◽  
pp. 747-752 ◽  
Author(s):  
Daiane D. Hartwig ◽  
Karine M. Forster ◽  
Thaís L. Oliveira ◽  
Marta Amaral ◽  
Alan J. A. McBride ◽  
...  
Keyword(s):  
Immune Response ◽  
Antibody Response ◽  
Dna Vaccine ◽  
Subunit Vaccine ◽  
Vaccine Candidate ◽  
Virulent Strain ◽  
Effective Vaccine ◽  
Pairwise Identity ◽  
Content Type ◽  
Vaccine Preparation

ABSTRACTToward developing an effective vaccine capable of conferring heterologous protection, the putative lipoprotein LemA, which presents an M3 epitope similar to that ofListeria, was evaluated as a vaccine candidate in the hamster model of leptospirosis. LemA is conserved (>70% pairwise identity) among the pathogenicLeptospiraspp., indicating its potential in stimulating a cross-protective immune response. Using different vaccination strategies, including prime-boost, DNA vaccine, and a subunit preparation, recombinant LemA conferred different levels of protection in hamsters. Significant protection against mortality was observed for the prime-boost and the DNA vaccine strategies, which showed 87.5% (P< 0.01) and 62.5% (P< 0.05) efficacy, respectively. Although the subunit vaccine preparation protected 50.0% of immunized hamsters, the level of protection was not significant. None of the hamsters in the control groups survived challenge with a virulent strain ofLeptospira interrogansserogroup Icterohaemorrhagiae. Characterization of the immune response found that the strongest antibody response was stimulated by the subunit vaccine preparation, followed by the prime-boost strategy. The DNA vaccine failed to elicit an antibody response in immunized hamsters.

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