Antimicrobial resistance, virulence genes, and biofilm formation in Staphylococcus aureus strains isolated from meat and meat products

2021 ◽  
Author(s):  
Kobra Abbasi ◽  
Elahe Tajbakhsh ◽  
Hassan Momtaz
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Meat Products

scholarly journals Enterotoxins production, biofilm formation and antimicrobial resistance of Staphylococcus aureus strains isolated from refrigerated raw cow milk

2019 ◽  
Vol 42 ◽  
pp. e45231
Author(s):  
Camila Lampugnani ◽  
Maike Taís Maziero Montanhini ◽  
Maria Emilene Martino Campos‐Galvão ◽  
Luis Augusto Nero ◽  
Luciano dos Santos Bersot
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Raw Milk ◽  
Cow Milk ◽  
In Vitro Assays ◽  
Vitro Assay ◽  
Milk Samples ◽  
Adherence Ability

This study aimed to isolate Staphylococcus aureus in refrigerated raw cow milk, and identify the presence of enterotoxin-expression genes, enterotoxin production and adherence ability, and antimicrobial resistance potential of the isolated strains. Fifty raw milk samples obtained in different dairy farms were analyzed for S. aureus and evaluated in the isolates the presence of genes associated with the production of major staphylococcal enterotoxins and biofilm formation. In vitro assays were also performed to evaluate the production of enterotoxins and adherence ability, and the antimicrobial resistance. One half (25/50) of raw milk samples presented coagulase-positive staphylococci and 95.2% of the isolates were confirmed to be S. aureus. Among them, 42.4% were carrying genes for enterotoxins production; however, only one isolate was able to produce enterotoxins. All S. aureus isolates were carrying at least two genes associated with biofilm formation and 95.2% isolates was able to adhere upon the in vitro assay. All isolates demonstrated antimicrobial resistance potential to one or more of the tested antibiotics.

scholarly journals Virulence genes and antimicrobial resistance profiles of Staphylococcus aureus isolated from chicken meat in Isfahan province, Iran

2013 ◽  
Vol 22 (4) ◽  
pp. 913-921 ◽  
Author(s):  
Hassan Momtaz ◽  
Farhad Safarpoor Dehkordi ◽  
Ebrahim Rahimi ◽  
Amin Asgarifar ◽  
Mahmood Momeni
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Chicken Meat ◽  
Isfahan Province

scholarly journals Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine

2021 ◽  
Vol 888 (1) ◽  
pp. 012064
Author(s):  
M H Chai ◽  
M Z Sukiman1 ◽  
Y F Chan ◽  
Y W Liew ◽  
L Z H Lai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Peninsular Malaysia ◽  
Health Concern ◽  
Molecular Characteristics ◽  
Multiple Drug ◽  
Multiple Drug Resistant ◽  
Significant Public Health ◽  
Amoxicillin Clavulanate

Abstract The emergence of bacteria with antimicrobial resistance traits such as methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is a significant public health concern. The aim of this study is to determine the prevalence rate, antibiotic susceptibility and the molecular characteristics of Staphylococcus aureus (S. aureus) in swine from selected swine farms in Peninsular Malaysia. The oral and nasal swabs of 200 live swine were collected. Screening and isolation of S. aureus isolates were carried out using phenotypic identification techniques. The identity of the bacteria isolates was confirmed by detection of nuc gene (S. aureus) and mecA gene (MRSA). Antibiogram of S. aureus against 19 antibiotics was developed using Kirby-Bauer test. Molecular detections of antimicrobial resistance and virulence genes as well spa typing of S. aureus was carried out. 25% of swine were carrying S. aureus with 9% being MRSA carriers. S. aureus and MRSA have shown high resistance against clindamycin, tetracycline, chloramphenicol, oxacillin, penicillin, erythromycin, cefoxitin and amoxicillin/clavulanate. Antimicrobial resistance and virulence genes, including tetK, tetL, tetM, ermA, ermB, ermC, msrA and scn genes were detected. 93% of S. aureus were multiple-drug resistant (MDR), suggesting the emergence of MDR S. aureus in swine in Malaysia.

Staphylococcus aureus isolated from handmade sweets: Biofilm formation, enterotoxigenicity and antimicrobial resistance

2016 ◽  
Vol 58 ◽  
pp. 105-111 ◽  
Author(s):  
Isabela Schneid Kroning ◽  
Mariana Almeida Iglesias ◽  
Carla Pohl Sehn ◽  
Tatiane Kuka Valente Gandra ◽  
Marcia Magalhães Mata ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Biofilm Formation

scholarly journals Antibiofilm effects of N,O-acetals derived from 2-amino-1,4-naphthoquinone are associated with downregulation of important global virulence regulators in methicillin-resistant Staphylococcus aureus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Juliana Silva Novais ◽  
Mariana Fernandes Carvalho ◽  
Mariana Severo Ramundo ◽  
Cristiana Ossaille Beltrame ◽  
Reinaldo Barros Geraldo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Biological Properties ◽  
New Drugs ◽  
Strong Impact ◽  
Methicillin Resistant ◽  
Great Demand

AbstractDespite the existing antibiotics, antimicrobial resistance is a major challenge. Consequently, the development of new drugs remains in great demand. Quinones is part of a broad group of molecules that present antibacterial activity besides other biological properties. The main purpose of this study was to evaluate the antibiofilm activities of synthetic N,O-acetals derived from 2-amino-1,4-naphthoquinone [7a: 2-(methoxymethyl)-amino-1,4-naphthoquinone; 7b: 2-(ethoxymethyl)-amino-1,4-naphthoquinone; and 7c: 2-(propynyloxymethyl)-amino-1,4-naphthoquinone] against methicillin-resistant Staphylococcus aureus (MRSA). The derivatives 7b and 7c, specially 7b, caused strong impact on biofilm accumulation. This inhibition was linked to decreased expression of the genes fnbA, spa, hla and psmα3. More importantly, this downregulation was paralleled by the modulation of global virulence regulators. The substitution of 2-ethoxymethyl (7b) in comparison with 2-propynyloxymethyl (7c) enhanced sarA-agr inhibition, decreased fnbA transcripts (positively regulated by sarA) and strongly impaired biofilm accumulation. Indeed, 7b triggered intensive autolysis and was able to eliminate vancomycin-persistent cells. Consequently, 7b is a promising molecule displaying not only antimicrobial effects, but also antibiofilm and antipersistence activities. Therefore, 7b is a good candidate for further studies involving the development of novel and more rational antimicrobials able to act in chronic and recalcitrant infections, associated with biofilm formation.

scholarly journals Resistance Profile From Staphylococcus Aureus And Pseudomonas Aeruginosa Obtained From Tracheostomized Children

2020 ◽  
Author(s):  
Juliana Afonso de Almeida ◽  
Caroline Espíndola de Barros ◽  
Gustavo Henrique da Silva Ayres ◽  
Mariana Helena e Silva ◽  
Andressa Santos Liberal ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Resistance ◽  
Congo Red ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Molecular Profile ◽  
Resistance Profile ◽  
Phenotypic Tests ◽  
Tracheal Secretion

Abstract Background The tracheostomized patients exhibit high risks of bacterial infections, because the tracheal tube acts as a gateway to these microorganisms. The objective was to characterize microbiologically the tracheal secretion of tracheostomized children, to evaluate the biofilm formation, and to study the phenotypic and molecular profile of antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated. Methods The study collected 88 tracheal secretion samples. The material processed by phenotypic tests were performed for bacterial identification. For identification of the biofilm, the Congo red agar test and the plaque microtiter test were used, and the qPCR method was used to resistance verification. Results Were obtained 27 samples of S. aureus and 71 of P. aeruginosa . All S. aureus samples were positive for biofilm formation on Congo red agar test. In antibiogram test, S. aureus showed resistance to seven drugs. Regarding the identification of resistance genes, were amplificated bla Z in 42.8% from S. aureus and mec A in 28.6% of them. Pseudomonas aeruginosa presented resistance to eight drugs. The most frequent chromosomal genes were bla OXA with 66.7% and bla KPC with 58.3%. To plasmidial DNA, was highlighted bla NDM with 58.3% positive. Conclusion The S. aureus and P. aeruginosa characterization of colonization from lower respiratory tract associated with the use of the device in tracheotomy patients, and the physiology and antimicrobial resistance profile, will help health professionals to choose the most appropriate treatment to be administered in children with tracheotomy, increasing the chances of airway restoration and decannulation.

scholarly journals Antimicrobial resistance, virulence genes and biofilm formation in Enterococcus species isolated from milk of sheep and goat with subclinical mastitis

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259584
Author(s):  
Mona A. El-Zamkan ◽  
Hams M. A. Mohamed
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Subclinical Mastitis ◽  
Virulence Determinants ◽  
Antimicrobial Susceptibility Test ◽  
Rdna Sequencing ◽  
Dairy Animals ◽  
Mastitic Milk

This study is designed to discuss the antimicrobial resistance, virulence determinants and biofilm formation capacity of Enterococcus spp. isolated from milk of sheep and goat with subclinical mastitis in Qena, Egypt. The obtained isolates were identified by the VITEK2 system and 16S rDNA sequencing as E. faecalis, E. faecium, E. casseliflavus and E. hirae. Overall, E. faecalis and E. faecium were the dominant species recovered from mastitic milk samples. The antimicrobial susceptibility test evidenced multidrug resistance of the isolates against the following antimicrobials: oxacillin (89.2.%), followed by vancomycin (75.7%) and linezolid (70.3%). Also, most of these isolates (73%) could form biofilms. For example, 18.9% of Enterococcus strains formed strong biofilm, whereas 32.4% of isolates formed moderate biofilm and 21.6% of isolates formed weak biofilm. The most prevalent resistance genes found in our isolates were blaZ (54%), vanA (40%), ermB (51.4%), tetM (13.5%) and optrA (10.8%). Moreover, asa1 (37.8%), cylA (42.3%), gelE (78.4%), esp (32.4%), EF3314(48.6%) and ace (75.5%) were the most common virulence genes. A significant correlation was found between biofilm formation, multidrug resistance and virulence genes of the isolates. This study highlights several aspects of virulence and harmfulness of Enterococcus strains isolated from subclinical mastitic milk, which necessitates continuous inspection and monitoring of dairy animals.

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