Ochratoxin A and zearalenone in poultry feed samples from South China

2021 ◽  
Author(s):  
Qiongshan Wang ◽  
Yarong Zhao ◽  
Peirong Chen ◽  
Rui Zeng ◽  
Yunxiang Liang
Keyword(s):  
Ochratoxin A ◽  
South China ◽  
Poultry Feed ◽  
Feed Samples

scholarly journals Fungal contamination and natural occurrence of ochratoxin A (OTA) in poultry feed

2014 ◽  
Vol 30 (3) ◽  
pp. 481-488 ◽  
Author(s):  
V. Krnjaja ◽  
Z. Pavlovski ◽  
M. Lukic ◽  
Z. Skrbic ◽  
Lj. Stojanovic ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Laying Hens ◽  
Enzymatic Assay ◽  
Poultry Feed ◽  
Natural Occurrence ◽  
Fungal Contamination ◽  
Penicillium Species ◽  
Toxigenic Fungi ◽  
Feed Samples

Total fungal count, the presence of potentially toxigenic fungi and natural occurrence of ochratoxin A (OTA) were studied in 30 poultry feed samples (14 samples of feed for chickens and 16 samples of feed for laying hens), which were collected from different farms in Serbia at the beginning of year 2014. The total number of fungi was determined by the method of dilution and OTA was detected using the imunoadsorption enzymatic assay (ELISA). In most of the samples of chickens feed (50%) the total number of fungi was 1 - 3 x 102 CFU g-1, and in feed for laying hens the highest number of samples (37.50%) had the total fungal count from 1.4 to 4.8 x 104 CFU g -1. The species of genera Aspergillus and Penicillium were identified as producers of OTA in 21.43% and 42.86% of chickens feed samples and in 68.75% and 25% of samples of feed for laying hens. The presence of OTA was detected in 100% of samples of feed for chickens and laying hens, with average concentrations of 34.40 ?g kg-1 (feed for chickens) and 43.89 ?g kg-1 (feed for laying hens). The total fungal count and content of OTA were not above the maximum allowed quantities, even though the presence of Aspergillus and Penicillium species was found in a large number of samples (up to 68.75%). These results indicate that the tested samples of poultry feed were mycologically and mycotoxicologically correct.

scholarly journals Monitoring the Presence and Investigation of Toxigenic Fungi and Mycotoxins in Poultry Feed and its Products

2020 ◽  
Author(s):  
Dana Abdalla Aboumaalie ◽  
Samir Jaoua
Keyword(s):  
Detection Limit ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Poultry Feed ◽  
The European Union ◽  
Toxigenic Fungi ◽  
Aflatoxin Concentration ◽  
High Concentrations ◽  
Almost All ◽  
Feed Samples

Contaminating poultry feed and their products with mycotoxins produced by fungi may cause many health effects on animals and human if they were at high concentrations. Therefore, it is imperative to regularly monitor the concentration of mycotoxins specially aflatoxin and ochratoxin A in the poultry feed and their products. In the present study, we demonstrated that Aspergillus flavus was the major contaminant using DNA extraction and gel electrophoresis. Using ELISA kit for ochratoxin A, Ochratoxin A did not exceed the detection limit 50 ng/kg but in one sample has exceeded the European Union maximum limit for aflatoxins of 20 μg/kg through the ELISA aflatoxin All kit. Aflatoxin B1 was detected in chicken liver samples using ELISA aflatoxin B1. Almost all samples were contaminated with fungi but only 4 feed samples showed aflatoxin concentration within the detection limit. Furthere experiments should be done on different liver samples in Qatar to chek the probability of this presence.

Determination of Ochratoxin A in Feed by Immunoaffinity Cleanup and Liquid Chromatography

2013 ◽  
Vol 96 (3) ◽  
pp. 599-602 ◽  
Author(s):  
Ping Ding ◽  
Ziyou Mi ◽  
Yali Hou ◽  
Yigang He ◽  
Jianhua Xie
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Ochratoxin A ◽  
Cyanogen Bromide ◽  
Polyclonal Antibodies ◽  
Immunoaffinity Column ◽  
Low Levels ◽  
Sepharose 4B ◽  
Feed Samples

Abstract A method using LC was developed for determination of ochratoxin A (OTA) in feeds. The extracted samples were cleaned up by an immunoaffinity column prepared by covalently coupling polyclonal antibodies against OTA to cyanogen bromide-activated Sepharose 4B. The eluates were determined by LC with fluorescence detection. Recoveries of OTA from fortified samples of 1–10 μg/kg levels ranged from 84.3 to 90.0%, with CVs of 3.3–7.8%. The detection limit was 0.045 μg/kg based on an S/N of 3:1. A total of 65 feed samples were screened for OTA with the proposed method. The results showed that only nine samples were contaminated with OTAs at low levels. The presented method was successfully applied to quantify OTAs in real feed samples.

scholarly journals Effect of relative humidity on biodeterioration of poultry feed and ochratoxin A production by Penicillium species

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
V. Koteswara Rao ◽  
B. Aruna ◽  
Md. Rafiyuddin ◽  
K. Narasimha Rao ◽  
S. Girisham ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Ochratoxin A ◽  
Poultry Feed ◽  
Penicillium Species

Improved Spectrophotometric Determination of Cyclopiazonic Acid in Poultry Feed and Corn

1990 ◽  
Vol 73 (2) ◽  
pp. 257-259 ◽  
Author(s):  
Ivan Chang-Yen ◽  
Keshore Bidasee
Keyword(s):  
Detection Limit ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Cyclopiazonic Acid ◽  
Poultry Feed ◽  
Reaction Conditions ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Feed Samples

Abstract An improved visible spectrophotometric method has been developed for cyclopiazonic acid in poultry feed and corn. The method Is based on the reaction of cyclopiazonic acid with Ehrlich reagent and detection at 580 nm. Reaction conditions were optimized with respect to reaction and measurement times and acid and Ehrlich reagent concentrations. Calibration curves were linear from 1 to 20 μg cyclopiazonic acid in 3 mL Ehrlich reagent, with a lower detection limit of 0.08 mg/kg for 50 g samples of poultry feed and corn. Recoveries from 50 g samples of poultry feed spiked with cyclopiazonic ranging from 0.16 to 1.20 mg/kg averaged 93.8%. Moldy corn and poultry feed samples analyzed by this method contained between 1 and 4 mg/kg cyclopiazonic acid.

scholarly journals Analysis of Mycotoxins Contamination in Poultry Feeds Manufactured in Selected Provinces of South Africa Using UHPLC-MS/MS

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 452 ◽  
Author(s):  
Sharon Maphala Mokubedi ◽  
Judith Zanele Phoku ◽  
Rumbidzai Naledi Changwa ◽  
Sefater Gbashi ◽  
Patrick Berka Njobeh
Keyword(s):  
South Africa ◽  
High Performance ◽  
Fumonisin B1 ◽  
Monomethyl Ether ◽  
Poultry Feed ◽  
Alternariol Monomethyl Ether ◽  
Triple Quadrupole Mass Spectrometer ◽  
Type A Trichothecenes ◽  
Contamination Levels ◽  
Feed Samples

A total of 105 different types of poultry feed samples from South Africa were simultaneously analysed for the presence of 16 mycotoxins using ultra-high-performance liquid chromatography coupled to a triple quadrupole mass spectrometer (UHPLC-MS/MS). The data revealed the presence of 16 mycotoxins in the various poultry feed samples. Fumonisin B1 (FB1) was the most dominant recovered from 100% of samples analysed at concentrations ranging between 38.7 and 7125.3 µg/kg. This was followed by zearalenone (ZEN) (range: 0.1–429 µg/kg) and deoxynivalenol (DON) (range: 2.5–154 µg/kg). Samples were also found to be contaminated with fumonisin B2 (FB2) (range: 0.7–125.1 µg/kg), fumonisin B3 (FB3) (range: 0.1–125.1 µg/kg), α-zearalenol (α-ZEL) (range: 0.6–20 µg/kg ), β-zearalenol (β-ZEL) (range: 0.2–22.1 µg/kg), 3-acetyldeoxynivalenol (3-ADON) (range: 0.1–12.9 µg/kg) and 15-acetyldeoxynivalenol (15-ADON) (range: 1.7–41.9 µg/kg). Alternaria mycotoxin, i.e., Alternariol monomethyl ether (AME) was recovered in 100% of samples at concentrations that ranged from 0.3–155.5 µg/kg. Aflatoxins (AFs) had an incidence rate of 92% with generally low concentration levels ranging from 0.1–3.7 µg/kg. Apart from these metabolites, 2 type A trichothecenes (THs), i.e., HT-2 toxin (HT-2) (range: 0.2–5.9 µg/kg) and T-2 toxin (T-2) (range: 0.1–15.3 µg/kg) were also detected. Mycotoxin contamination in South African poultry feed constitutes a concern as correspondingly high contamination levels, such as those observed herein are likely to affect birds, which can be accompanied by severe health implications, thus compromising animal productivity in the country. Such exposures, primarily to more than one mycotoxin concurrently, may elicit noticeable synergistic and or additive effects on poultry birds.

Comparison of extraction techniques of robenidine from poultry feed samples

Talanta ◽  
2007 ◽  
Vol 73 (5) ◽  
pp. 812-819 ◽  
Author(s):  
J WILGA ◽  
A WASIK ◽  
J NAMIESNIK
Keyword(s):  
Poultry Feed ◽  
Extraction Techniques ◽  
Feed Samples

scholarly journals Detection of heavy metals in poultry feed, meat and eggs

2018 ◽  
Vol 2 (1) ◽  
pp. 1-5
Author(s):  
MA Rashid ◽  
MSK Sarker ◽  
H Khatun ◽  
NR Sarker ◽  
Md Yousuf Ali ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Regulatory Authority ◽  
Marketing Channel ◽  
The Other ◽  
Poultry Feed ◽  
Broiler Meat ◽  
Spent Hen ◽  
Metal Contents ◽  
Meat And Eggs ◽  
Feed Samples

The poultry feed, meat and egg samples were collected from the major poultry raising areas of the country to know the presence of heavy metals especially arsenic (As), lead (Pb) and chromium (Cr). A total of 360 elemental samples for As, Pb and Cr were analyzed in the laboratory. The heavy metal contents (As, Pb & Cr) of all the tested samples were found positive. But, the levels were below the Maximum Permitted Concentration (MPC) in most cases. The layer and broiler ready feed samples found safe from those elements; On the other hand, the “Cr” and “Pb” content in 14% and 11% loose feed samples were 7-70 and 3 times respectively higher than that of MPC. Notes worthy, the egg samples were also found safe from those elements. Of the tested samples 14% broiler meat and 50% spent hen samples found slightly higher level of “As” and 4-6 times “Cr” respectively than the MPC. In conclusion, the poultry farmers are suggested to buy poultry feeds from the reputed feed company and discourage to use loose feeds. Moreover, feed marketing channel particularly loose feed marketing should be monitored properly by the regulatory authority. Asian Australas. J. Food Saf. Secur. 2018, 2(1), 1-5

scholarly journals Production of Penicillic Acid and Ochratoxin A on Poultry Feed by Aspergillus ochraceus: Temperature and Moisture Requirements

1973 ◽  
Vol 26 (2) ◽  
pp. 155-160
Author(s):  
C. W. Bacon ◽  
J. G. Sweeney ◽  
J. D. Robbins ◽  
D. Burdick
Keyword(s):  
Ochratoxin A ◽  
Aspergillus Ochraceus ◽  
Poultry Feed ◽  
Penicillic Acid

scholarly journals Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Toxins ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 317 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Hui Xie ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Ochratoxin A ◽  
Limit Of Detection ◽  
Screen Printed Electrodes ◽  
Combined Use ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Liquid Chromatography Tandem Mass ◽  
Feed Samples ◽  
Screen Printed

A rapid and sensitive electrochemical biosensor based on magnetic nanoparticles and screen-printed electrodes (MNPs-SPEs sensor) was developed for the detection of ochratoxin A (OTA) in cereal and feed samples. Different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) were optimized, and the signal detection, as well as sensitivity, was enhanced by the combined use of screen-printed electrodes (SPEs). Under the optimized conditions, the calibration curve of the MNPs-SPEs sensor was y = 0.3372x + 0.8324 (R2 = 0.9805). The linear range of detection and the detection limit were 0.01–0.82 ng/mL and 0.007 ng/mL, respectively. In addition, 50% inhibition (IC50) was detectable at 0.10 ng/mL. The limit of detection (LOD) of this MNPs-SPEs sensor in cereal and feed samples was 0.28 μg/kg. The recovery rates in spiked samples were between 78.7% and 113.5%, and the relative standard deviations (RSDs) were 3.6–9.8%, with the coefficient of variation lower than 15%. Parallel analysis of commercial samples (corn, wheat, and feedstuff) showed a good correlation between MNPs-SPEs sensor and liquid chromatography tandem mass spectrometry (LC/MS-MS). This new method provides a rapid, highly sensitive, and less time-consuming method to determine levels of ochratoxin A in cereal and feedstuff samples.

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