The liquid Kangfuxin (KFX) has efficient antifungal activity and can be used in the treatment of vulvovaginal candidiasis in mouse

2021 ◽  
Author(s):  
Jia Ma ◽  
Ya‐Chao Yang ◽  
Liu‐Qing Su ◽  
Ding‐Mei Qin ◽  
Kai Yuan ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Vulvovaginal Candidiasis

Spilanthol as a promising antifungal alkylamide for the treatment of vulvovaginal candidiasis

2021 ◽  
Author(s):  
Rodrigo L Fabri ◽  
Jhamine C O Freitas ◽  
Ari S O Lemos ◽  
Lara M Campos ◽  
Irley O M Diniz ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Cell Membrane ◽  
Multidrug Resistant ◽  
Fungal Strain ◽  
Vulvovaginal Candidiasis ◽  
Biofilm Matrix

Abstract Spilanthol is a bioactive alkylamide from the native Amazon plant species, Acmella oleracea. However, antifungal activities of spilanthol and its application to the therapeutic treatment of candidiasis remains to be explored. This study sought to evaluate the in vitro and in vivo antifungal activity of spilanthol previously isolated from A. oleracea (spilanthol(AcO)) against Candida albicans ATCC® 10231™, a multidrug-resistant fungal strain. Microdilution methods were used to determine inhibitory and fungicidal concentrations of spilanthol(AcO). In planktonic cultures, the fungal growth kinetics, yeast cell metabolic activity, cell membrane permeability and cell wall integrity were investigated. The effect of spilanthol(AcO) on the proliferation and adhesion of fungal biofilms was evaluated by whole slide imaging and scanning electron microscopy. The biochemical composition of the biofilm matrix was also analyzed. In parallel, spilanthol(AcO) was tested in vivo in an experimental vulvovaginal candidiasis model. Our in vitro analyses in C. albicans planktonic cultures detected a significant inhibitory effect of spilanthol(AcO), which affects both yeast cell membrane and cell wall integrity, interfering with the fungus growth. C. albicans biofilm proliferation and adhesion, as well as, carbohydrates and DNA in biofilm matrix were reduced after spilanthol(AcO) treatment. Moreover, infected rats treated with spilanthol(AcO) showed consistent reduction of both fungal burden and inflammatory processes compared to the untreated animals. Altogether, our findings demonstrated that spilanthol(AcO) is an bioactive compound against planktonic and biofilm forms of a multidrug resistant C. albicans strain. Furthermore, spilanthol(AcO) can be potentially considered for therapeutical treatment of vulvovaginal candidiasis caused by C. albicans. Lay Abstract This study sought to evaluate the antifungal activity of spilanthol against Candida albicans ATCC® 10 231™, a multidrug-resistant fungal strain. Our findings demonstrated that spilanthol(AcO) can be potentially considered for therapeutical treatment of vulvovaginal candidiasis caused by C. albicans.

scholarly journals Antifungal activity of Ocimum gratissimum L., Lantana camara L. & Pteleopsis suberosa Engl. & Dies used in the treatment of vulvovaginal candidiasis in Benin

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jean Robert Klotoe ◽  
Brice Armand Fanou ◽  
Eric Agbodjento ◽  
Arnaud Houehou ◽  
Lauris Fah ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aqueous Extract ◽  
Lantana Camara ◽  
Vulvovaginal Candidiasis ◽  
Reference Drug ◽  
Clinical Strains ◽  
Ocimum Gratissimum

Abstract Background Vulvovaginal candidiasis is a widespread mycotic infection that affects a large proportion of women of childbearing age. Its management in traditional medicine is based on the use of medicinal plants. This study aimed to evaluate the antifungal activity of Ocimum gratissimum L., Lantana camara L. and Pteleopsis suberosa Engl. & Diels used in the treatment of vulvovaginal candidiasis in Benin. Results The data obtained from the in vitro antifungal test show that the strains tested (ATCC 90028 and two clinical strains: 1MA and 3MA) were more sensitive to aqueous extracts with a better effect for Pteleopsis suberosa. This potential of the tested extracts correlated with their richness in total polyphenols. The extract of the Pteleopsis suberosa was very active on the inhibition of the reference strain ATCC 90028. On the clinical strains (1MA and 3MA) the aqueous extract of Pteleopsis suberosa showed a better MIC on the 1MA strain. In vivo model, inoculation of 100 µL of the concentrated Candida albicans suspension 1.5 × 105 UFC/mL induced the candidiasis of the female Wistar rat. The treatment with the aqueous extract of Pteleopsis suberosa, like fluconazole (reference drug), significantly reduced Candida albicans infection at a dose of 100 mg/kg after 1, 7 and 13 days of treatment. Conclusion This study revealed the potential antifungal of the Ocimum gratissimum, Lantana camara and Pteleopsis suberosa. Pteleopsis suberosa has better antifungal activity in vitro and in vivo. These observations justify the use of their medicinal plant in the traditional treatment of vulvovaginal candidiasis in Benin.

scholarly journals Antifungal Activity of Brazilian Propolis Microparticles against Yeasts Isolated from Vulvovaginal Candidiasis

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Kelen Fátima Dalben Dota ◽  
Marcia Edilaine Lopes Consolaro ◽  
Terezinha Inez Estivalet Svidzinski ◽  
Marcos Luciano Bruschi
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
Biological Activities ◽  
Small Variation ◽  
Vulvovaginal Candidiasis ◽  
Antifungal Drugs ◽  
Broth Microdilution Method ◽  
Brazilian Propolis ◽  
Dose Dependent

Propolis, a resinous compound produced byApis melliferaL. bees, is known to possess a variety of biological activities and is applied in the therapy of various infectious diseases. The aim of this study was to evaluate thein vitroantifungal activity of propolis ethanol extract (PE) and propolis microparticles (PMs) obtained from a sample of Brazilian propolis against clinical yeast isolates of importance in the vulvovaginal candidiasis (VVC). PE was used to prepare the microparticles. Yeast isolates (n=89), obtained from vaginal exudates of patients with VVC, were exposed to the PE and the PMs. Moreover, the main antifungal drugs used in the treatment of VVC (Fluconazole, Voriconazole, Itraconazole, Ketoconazole, Miconazole and Amphotericin B) were also tested. Minimum inhibitory concentration (MIC) was determined according to the standard broth microdilution method. SomeCandida albicansisolates showed resistance or dose-dependent susceptibility for the azolic drugs and Amphotericin B. Non-C. albicansisolates showed more resistance and dose-dependent susceptibility for the azolic drugs thanC. albicans. However, all of them were sensitive or dose-dependent susceptible for Amphotericin B. All yeasts were inhibited by PE and PMs, with small variation, independent of the species of yeast. The overall results provided important information for the potential application of PMs in the therapy of VVC and the possible prevention of the occurrence of new symptomatic episodes.

scholarly journals Antifungal Activity of ZnO Nanoparticles and Nystatin and Downregulation of SAP1-3 Genes Expression in Fluconazole-Resistant Candida albicans Isolates from Vulvovaginal Candidiasis

2020 ◽  
Vol Volume 13 ◽  
pp. 385-394 ◽  
Author(s):  
Seyedeh Sedigheh Hosseini ◽  
Hamidreza Joshaghani ◽  
Tahereh Shokohi ◽  
Alireza Ahmadi ◽  
Zahra Mehrbakhsh
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Zno Nanoparticles ◽  
Vulvovaginal Candidiasis ◽  
Genes Expression ◽  
Fluconazole Resistant

scholarly journals Inhibitory effects of Lactobacillus casei Shirota against both Candida auris and Candida spp. isolates that cause vulvovaginal candidiasis and are resistant to antifungals

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Aline Lorenzoni Paniágua ◽  
Amabel Fernandes Correia ◽  
Lívia Custódio Pereira ◽  
Bruna Maciel de Alencar ◽  
Fabiana Brandão Alves Silva ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Antifungal Activity ◽  
Lactobacillus Casei ◽  
Vulvovaginal Candidiasis ◽  
Reproductive Age ◽  
Dilution Method ◽  
Inhibitory Effects ◽  
Candida Auris ◽  
Candida Spp ◽  
Physiological Concentration

Abstract Background Vulvovaginal candidiasis (VVC), the second leading cause of genital infection in women of reproductive age, is caused by yeasts of the genus Candida. Treatment is usually empirical and performed with azoles, which have shown increasing ineffectiveness due to resistance from these species. This therapeutic challenge has led to the search for new treatment strategies. Lactobacillus spp. produce several components with microbicidal effects, such as lactic acid. These species are the main components of a healthy vaginal microbiota and have been used as probiotics. The aim of this work was to investigate the in vitro inhibitory effects of Lactobacillus casei Shirota on both the Candida spp. that cause VVC and on C. auris. Methods The microbicidal effects of L. casei Shirota on the main VVC-causing species, C. albicans, C. tropicalis, C. norvegensis and C. parapsilosis, in addition to C. auris were investigated by counting the Colony-forming Units (CFUs) after cocultivation. The antifungal activity of lactic acid against these Candida strains was assessed using the microtiter broth dilution method to determine the minimum inhibitory concentrations (MICs). The effects of L. casei Shirota on hyphal and early biofilm formation was measured by optical microscopy. Results L. casei Shirota showed inhibitory action against all tested Candida spp., ranging from 66.9 to 95.6% inhibition depending on the species. This inhibition is possibly related to the production of lactic acid, since lactic acid has shown microbicidal action against these same Candida spp. at a concentration of 5 mg/mL, which corresponds to half of the normal physiological concentration. In addition, L. casei Shirota was able to reduce the formation of C. albicans hyphae and early biofilms, showing strong anti-Candida effects. Conclusions These results suggest that L. casei Shirota has antifungal activity against the Candida species that cause VVC. L. casei also has microbicidal action against C. auris.

scholarly journals Antifungal Activity of Natural Compounds vs. Candida spp.: A Mixture of Cinnamaldehyde and Eugenol Shows Promising In Vitro Results

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Ilaria Maria Saracino ◽  
Claudio Foschi ◽  
Matteo Pavoni ◽  
Renato Spigarelli ◽  
Maria Chiara Valerii ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Antifungal Activity ◽  
Synergistic Effect ◽  
Fungal Infections ◽  
Natural Compounds ◽  
Vulvovaginal Candidiasis ◽  
Total Loss ◽  
Diffusion Method ◽  
Candida Spp

Candida spp. are commensal organisms of the skin, mucous membranes, gastrointestinal tract, blood, and vagina of animals and humans. In recent decades, the incidence of human fungal infections has increased, with Candida spp. (mainly C. albicans) infections being the most frequent, and the treatment of fungal infections is still a clinical challenge. Colonization of the human gastrointestinal tract by Candida spp. is significant because infections (e.g., candidemia and vulvovaginal candidiasis) frequently arise from commensal microorganisms. The aim of this study was to test in vitro the antifungal activity and the eventual synergistic effect of five pure components of essential oils: cinnamaldehyde, α-pinene, limonene, eucalyptol, and eugenol. These compounds were tested on 18 Candida strains (15 C. albicans, 2 C. glabrata, and 1 C. lusitaniae) derived from a culture collection of vaginal clinical strains. Methods: Fungistatic activity was evaluated using the disk diffusion method. For fungicidal activity, microdilution and time–kill curve protocols were set up. The checkerboard method was chosen to evaluate a possible synergistic effect of these compounds when mixed. Results: Cinnamaldehyde and eugenol gave the best results, inhibiting all the Candida strains and showing a highly additive effect (FICI 0.625). The cinnamaldehyde inhibition zone (IZ), MIC, and MFC means were 69 mm, 50.05 mg/L, and 109.26 mg/L respectively. Cinnamaldehyde led to the total loss of viable Candida cells within 4 h. Eugenol IZ, MIC, and MFC means were 35.2 mm, 455.42 mg/L, and 690.09 mg/L, respectively. Eugenol led to the total loss of viable fungal cells within 1 h. Treatment with α-pinene inhibited 88.9% of Candida strains, with an IZ mean of 21.2 mm, a MIC mean of 195.41 mg/L, and a MFC mean of 251.27 mg/L; this compound led to the total loss of viable fungal cells only after 24 h. Limonene inhibited only 33.3% of the tested strains and eucalyptol did not produce an inhibition halo, so these compounds were not tested further. Conclusions: These characteristics, together with the well-known safety of cinnamaldehyde and eugenol for human use, make these two natural compounds the perfect candidates for the treatment of candidiasis. This was a pilot study, the purpose of which was to evaluate the best composition of a mixture to be used against intestinal and vulvovaginal candidiasis; in vivo studies are needed to confirm these very encouraging results.

Evaluation of the antifungal activity of an Ebselen-loaded Nanoemulsion in a mouse model of vulvovaginal candidiasis

2021 ◽  
pp. 102428
Author(s):  
Suvidha Menon ◽  
Richa Vartak ◽  
Ketankumar Patel ◽  
Blase Billack
Keyword(s):  
Antifungal Activity ◽  
Mouse Model ◽  
Vulvovaginal Candidiasis

In vitro Antifungal Activity of Irlbachia purpurascens, Lantana macrophylla and Kielmeyera neglecta Extracts Against Candida Isolates Collected from Patients with Vulvovaginal Candidiasis

2013 ◽  
Vol 7 (3) ◽  
pp. 141-149 ◽  
Author(s):  
J.L. Andrioli ◽  
G.S.A. Oliveira ◽  
F.F. de Oliveira ◽  
M.C.H. de Oliveira ◽  
A.P.T. Uetanabaro ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Vulvovaginal Candidiasis ◽  
In Vitro Antifungal Activity

In vivo antifungal activity of nanoemulsions containing eucalyptus or lemongrass essential oils in murine model of vulvovaginal candidiasis

2020 ◽  
Vol 57 ◽  
pp. 101762 ◽  
Author(s):  
Samanta da Silva Gündel ◽  
Samantha Nunes de Godoi ◽  
Roberto Christ Vianna Santos ◽  
Jacqueline Teixeira da Silva ◽  
Liliana Borges de Menezes Leite ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Murine Model ◽  
Vulvovaginal Candidiasis ◽  
In Vivo Antifungal Activity
Sign in / Sign up

Export Citation Format

Share Document