Effect of Methylphenidate at Different Doses on New Bone Formation with Rapid Maxillary Expansion: A Micro‐CT and Histomorphometric Study in Rats

The purpose of this article was to evaluate the bone induction effects of an amnion membrane–protected graft compared with a collagen membrane–protected graft in the repair of tibial bony defects in dogs. This study was performed using the tibial bone of dogs. After the removal of periosteum, similar holes were made with a 16-mm trephine drill (38 holes in total). For the study group, 10 holes were covered by absorbable collagen and 16 holes by amniotic membrane. In the control group, 12 holes were made and covered by the overlying soft tissue. Tibial bones were exposed after 6 and 12 weeks, and the samples were harvested and histologically processed. New bone formation was evaluated by histomorphometric study. Four Iranian mixed dogs older than 1.5 years were included in this study. The new bone formation was less in the control group when compared with the collagen group (P = .863). The collagen group showed less bone formation than the amnion group (P = .194), but this difference was not significant. However, bone formation in the amnion group was significantly more than in the control group (P = .050). Using the amniotic membrane appears to accelerate bone formation in guided bone regeneration. However, further studies should investigate its clinical impact on bone healing.

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Micro-CT Assessment of LED Irradiation on New Bone Formation in an Experimental Rat Calvaria Periosteal Elevation Model

Nippon Laser Igakkaishi ◽

10.2530/jslsm.jslsm-35_0021 ◽

2014 ◽

Vol 35 (2) ◽

pp. 151-157 ◽

Cited By ~ 1

Author(s):

Noboru Kuboyama ◽

K. Bhawal Ujjal ◽

Ryoichiro Uchida ◽

Akira Kaneda ◽

Yoshimitsu Abiko

Keyword(s):

Bone Formation ◽

Micro Ct ◽

New Bone Formation ◽

Rat Calvaria ◽

Elevation Model ◽

Led Irradiation

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STAT3 phosphorylation inhibition for treating inflammation and new bone formation in ankylosing spondylitis

Rheumatology ◽

10.1093/rheumatology/keaa846 ◽

2020 ◽

Author(s):

Sungsin Jo ◽

Eun Jeong Won ◽

Moon-Ju Kim ◽

Yu Jeong Lee ◽

So-Hee Jin ◽

...

Keyword(s):

Bone Formation ◽

Chronic Inflammation ◽

Mononuclear Cells ◽

Kinase Inhibitor ◽

Janus Kinase ◽

Micro Ct ◽

New Bone Formation ◽

Osteoprogenitor Cells ◽

Suppression Effect ◽

Stat3 Phosphorylation

Abstract Objective AS is a rheumatic disease characterized by chronic inflammation and bony ankylosis. This study was to evaluate whether a signal transducer and activator of transcription 3 phosphorylation inhibitor (stat3-p Inh) could treat both chronic inflammation and bone formation in AS. Methods Primary AS osteoprogenitor cells and spinal entheseal cells were examined for osteogenic differentiation. SF mononuclear cells (SFMCs) and lamina propria mononuclear cells (LPMCs) were obtained from AS patients. Inflammatory cytokine-producing cells were analysed using flow cytometry and ELISA. Female SKG mice were treated with stat3-p Inh, IL-17A blocker or vehicle. Inflammation and new bone formation were evaluated using immunohistochemistry, PET and micro-CT. Results In the SKG mouse model, stat3-p Inh significantly suppressed arthritis, enthesitis, spondylitis and ileitis. In experiments culturing SFMCs and LPMCs, the frequencies of IFN-γ-, IL-17A- and TNF-α-producing cells were significantly decreased after stat3-p Inh treatment. When comparing current treatments for AS, stat3-p Inh showed a comparable suppression effect on osteogenesis to Janus kinase inhibitor or IL-17A blocker in AS-osteoprogenitor cells. Stat3-p Inh suppressed differentiation and mineralization of AS-osteoprogenitor cells and entheseal cells toward osteoblasts. Micro-CT analysis of hind paws revealed less new bone formation in stat3-p Inh-treated mice than vehicle-treated mice (P = 0.005). Hind paw and spinal new bone formation were similar between stat3-p Inh- and anti-IL-17A-treated SKG mice (P = 0.874 and P = 0.117, respectively). Conclusion Stat-3p inhibition is a promising treatment for both inflammation and new bone formation in AS.

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Effects of Ginkgo biloba on experimental rapid maxillary expansion model: a histomorphometric study

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2012.03.007 ◽

2012 ◽

Vol 114 (6) ◽

pp. 712-718 ◽

Cited By ~ 7

Author(s):

Muhammed Isa Kara ◽

Ayşe Burcu Altan ◽

Ufuk Sezer ◽

Muharrem Şerif Erdoğan ◽

Sevinc Inan ◽

...

Keyword(s):

Ginkgo Biloba ◽

Rapid Maxillary Expansion ◽

Maxillary Expansion ◽

Histomorphometric Study ◽

Expansion Model

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Micro-CT Analysis of New Bone Formation Around Implant with Electrochemically Deposited Apatite

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.240-242.611 ◽

2003 ◽

Vol 240-242 ◽

pp. 611-614 ◽

Cited By ~ 1

Author(s):

Seiji Ban ◽

Norihiro Arimoto ◽

Shozo Tsuruta ◽

Hiroyuki Arikawa ◽

Takahito Kanie ◽

...

Keyword(s):

Bone Formation ◽

Micro Ct ◽

New Bone Formation ◽

Electrochemically Deposited ◽

Ct Analysis

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Mechanical Tension-Stress in Alveolar Cleft Repaired With Autogenous Bone in Canine Models

The Cleft Palate-Craniofacial Journal ◽

10.1177/10556656211018950 ◽

2021 ◽

pp. 105566562110189

Author(s):

Anqi Liu ◽

Jialiang Huang

Keyword(s):

Finite Element Analysis ◽

Bone Formation ◽

Three Dimensional ◽

New Bone Formation ◽

Maxillary Expansion ◽

Element Analysis ◽

Alveolar Cleft ◽

Graft Area ◽

Dimensional Finite Element ◽

Three Dimensional Finite Element

Objective: Cleft lip and/or palate is a common birth defect worldwide, always accompanied by alveolar cleft. However, the success rate of secondary alveolar bone grafting is unsatisfactory. Rapid maxillary expansion (RME) often used after bone transplantation provides functional stimulation for bone graft area. This study aimed to investigate the effect of RME force on the bone graft area and midpalatal suture, and screen out the most suitable loaded force and loaded teeth, so as to provide a reference for clinical treatment. Methods: Fourteen 24-week-old male beagles were assigned randomly to 3 groups: blank control, autogenous, and autogenous with RME. Three-dimensional finite element analysis was conducted to evaluate the distribution and value of the stress in the model. The maxillae were collected and subjected to radiography and helical computed tomography to evaluate new bone formation in the graft area. Van Gieson’s Picrofuchsin staining was performed for histomorphological observation. Results: After 8 weeks of RME treatment, new bone formation of the dogs was markedly accelerated, and bone resorption was significantly reduced compared with the untreated dogs or those only treated with autogenous iliac bone. The treatment with RME evidently made the bone trabecula more abundant and the area of bone formation larger. Three-dimensional finite element analysis showed that the clinical effect can be achieved by using canine teeth as the loaded teeth and applying force of 10 MPa. Conclusion: Rapid maxillary expansion after bone grafting had a positive effect on osteogenesis in a canine model of alveolar cleft.

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Dual Blockade of TNF and IL-17A Inhibits Inflammation and Structural Damage in a Rat Model of Spondyloarthritis

International Journal of Molecular Sciences ◽

10.3390/ijms23020859 ◽

2022 ◽

Vol 23 (2) ◽

pp. 859

Author(s):

Ihsan Hammoura ◽

Renee H. Fiechter ◽

Shaughn H. Bryant ◽

Susan Westmoreland ◽

Gillian Kingsbury ◽

...

Keyword(s):

Bone Formation ◽

Rat Model ◽

Structural Damage ◽

Structural Changes ◽

Ct Imaging ◽

Control Treatment ◽

Micro Ct ◽

New Bone Formation ◽

Dual Blockade ◽

Dual Inhibition

The tumor necrosis factor (TNF) and IL-23/IL-17 axes are the main therapeutic targets in spondyloarthritis. Despite the clinical efficacy of blocking either pathway, monotherapy does not induce remission in all patients and its effect on new bone formation remains unclear. We aimed to study the effect of TNF and IL-17A dual inhibition on clinical disease and structural damage using the HLA-B27/human β2-microglobulin transgenic rat model of SpA. Immunized rats were randomized according to arthritis severity, 1 week after arthritis incidence reached 50%, to be treated twice weekly for a period of 5 weeks with either a dual blockade therapy of an anti-TNF antibody and an anti-IL-17A antibody, a single therapy of either antibody, or PBS as vehicle control. Treatment-blinded observers assessed inflammation and structural damage clinically, histologically and by micro-CT imaging. Both single therapies as well as TNF and IL-17A dual blockade therapy reduced clinical spondylitis and peripheral arthritis effectively and similarly. Clinical improvement was confirmed for all treatments by a reduction of histological inflammation and pannus formation (p < 0.05) at the caudal spine. All treatments showed an improvement of structural changes at the axial and peripheral joints on micro-CT imaging, with a significant decrease for roughness (p < 0.05), which reflects both erosion and new bone formation, at the level of the caudal spine. The effect of dual blockade therapy on new bone formation was more prominent at the axial than the peripheral level. Collectively, our study showed that dual blockade therapy significantly reduces inflammation and structural changes, including new bone formation. However, we could not confirm a more pronounced effect of dual inhibition compared to single inhibition.

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