Bone regeneration by freeze-dried composite of octacalcium phosphate collagen and teriparatide

Introduction: Autologous bone grafting or various bone-regenerating materials are used to treat bone defects caused by tumor resection and accident trauma. Octacalcium phosphate, a reasonable bone regenerative material, activates osteoblasts. We fabricated a composite material, octacalcium phosphate/weakly denatured collagen, as a new scaffold. We aimed to investigate the osteoregenerative effect of the octacalcium phosphate/weakly denatured collagen scaffold (compared with that of weakly denatured collagen) in skull defects in a canine model. Methods: Atelocollagen was extracted from porcine skin via pepsin treatment. The weakly denatured collagen scaffold was fabricated with a freeze-dried and thermally crosslinked atelocollagen suspension at pH 7.4. Octacalcium phosphate was synthesized using Ca-acetate and NaH2PO4. Octacalcium phosphate particles (diameter, 199–298 µm) were mixed with a collagen matrix to fabricate an octacalcium phosphate/weakly denatured collagen scaffold. Bilateral defects (diameter, 10 mm; full-thickness) were induced in dog skulls, and the octacalcium phosphate/weakly denatured collagen and weakly denatured collagen scaffolds were implanted into the defects. Results: Eight weeks after implantations, bone regeneration was evaluated via histopathological analysis. It revealed osteoblast infiltration and osteoregeneration in all defects treated with the octacalcium phosphate/weakly denatured collagen scaffold. The defects treated with weakly denatured collagen scaffold or without any scaffold mostly contained connective tissue, with no neo-osteogenesis. Discussion: The novel octacalcium phosphate/weakly denatured collagen scaffold better promotes osteoregeneration than the weakly denatured collagen scaffold; this “in situ tissue engineering” approach is potentially clinically applicable for bone reconstruction.

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Involvement of distant octacalcium phosphate scaffolds in enhancing early differentiation of osteocytes during bone regeneration

Acta Biomaterialia ◽

10.1016/j.actbio.2021.05.017 ◽

2021 ◽

Author(s):

Shizu Saito ◽

Ryo Hamai ◽

Yukari Shiwaku ◽

Tomoka Hasegawa ◽

Susumu Sakai ◽

...

Keyword(s):

Bone Regeneration ◽

Octacalcium Phosphate ◽

Early Differentiation

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Synthetic Octacalcium Phosphate Augments Bone Regeneration Correlated with Its Content in Collagen Scaffold

Tissue Engineering Part A ◽

10.1089/ten.tea.2008.0141 ◽

2009 ◽

Vol 15 (1) ◽

pp. 23-32 ◽

Cited By ~ 39

Author(s):

Tadashi Kawai ◽

Takahisa Anada ◽

Yoshitomo Honda ◽

Shinji Kamakura ◽

Keiko Matsui ◽

...

Keyword(s):

Bone Regeneration ◽

Collagen Scaffold ◽

Octacalcium Phosphate

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Micro-Computed Tomography Analysis on Administration of Mesenchymal Stem Cells - Bovine Teeth Scaffold Composites for Alveolar Bone Tissue Engineering

Journal of Biomimetics Biomaterials and Biomedical Engineering ◽

10.4028/www.scientific.net/jbbbe.52.86 ◽

2021 ◽

Vol 52 ◽

pp. 86-96

Author(s):

Desi Sandra Sari ◽

Fourier Dzar Eljabbar Latief ◽

Ferdiansyah ◽

Ketut Sudiana ◽

Fedik Abdul Rantam

Keyword(s):

Computed Tomography ◽

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Regeneration ◽

Bone Growth ◽

Alveolar Bone ◽

Micro Computed Tomography ◽

Freeze Dried ◽

Significant Difference

The tissue engineering approach for periodontal tissue regeneration using a combination of stem cells and scaffold has been vastly developed. Mesenchymal Stem Cells (MSCs) seeded with Bovine Teeth Scaffold (BTSc) can repair alveolar bone damage in periodontitis cases. The alveolar bone regeneration process was analyzed by micro-computed tomography (µ-CT) to observe the structure of bone growth and to visualize the scaffold in 3-Dimensional (3D). The purpose of this study is to analyze alveolar bone regeneration by µ-CT following the combination of MSCs and bovine teeth scaffold (MSCs-BTSc) implantation in the Wistar rat periodontitis model. Methods. MSCs were cultured from adipose-derived mesenchymal stem cells of rats. BTSc was taken from bovine teeth and freeze-dried with a particle size of 150-355 µm. MSCs were seeded on BTSc for 24 hours and transplanted in a rat model of periodontitis. Thirty-five Wistar rats were made as periodontitis models with LPS induction from P. gingivalis injected to the buccal section of interproximal gingiva between the first and the second mandibular right-molar teeth for six weeks. There were seven groups (control group, BTSc group on day 7, BTSc group on day 14, BTSc group on day 28, MSCs-BTSc group on day 7, MSCs-BTSc group on day 14, MSCs-BTSc group on day 28). The mandibular alveolar bone was analyzed and visualized in 3D with µ-CT to observe any new bone growth. Statistical Analysis. Group data were subjected to the Kruskal Wallis test followed by the Mann-Whitney (p <0.05). The µ-CT qualitative analysis shows a fibrous structure, which indicates the existence of new bone regeneration. Quantitative analysis of the periodontitis model showed a significant difference between the control model and the model with the alveolar bone resorption (p <0.05). The bone volume and density measurements revealed that the MSCs-BTSc group on day 28 formed new bone compared to other groups (p <0.05). Administration of MSCs-BTSc combination has the potential to form new alveolar bone.

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Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.01.035 ◽

2020 ◽

Vol 149 ◽

pp. 1-10 ◽

Cited By ~ 10

Author(s):

Pawan Kumar ◽

Meenu Saini ◽

Brijnandan S. Dehiya ◽

Ahmad Umar ◽

Anil Sindhu ◽

...

Keyword(s):

Bone Regeneration ◽

In Vitro Biocompatibility ◽

Freeze Dried

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Octacalcium phosphate collagen composite (OCP/Col) enhance bone regeneration in a rat model of skull defect with dural defect

Heliyon ◽

10.1016/j.heliyon.2020.e03347 ◽

2020 ◽

Vol 6 (2) ◽

pp. e03347 ◽

Cited By ~ 1

Author(s):

Takashi Sasaki ◽

Kuniyasu Niizuma ◽

Atsushi Kanoke ◽

Keiko Matsui ◽

Shogo Ogita ◽

...

Keyword(s):

Bone Regeneration ◽

Rat Model ◽

Octacalcium Phosphate ◽

Dural Defect ◽

Skull Defect

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The Influence of Eggshell on Bone Regeneration in Preclinical In Vivo Studies

Biology ◽

10.3390/biology9120476 ◽

2020 ◽

Vol 9 (12) ◽

pp. 476

Author(s):

Horia Opris ◽

Cristian Dinu ◽

Mihaela Baciut ◽

Grigore Baciut ◽

Ileana Mitre ◽

...

Keyword(s):

Bone Regeneration ◽

Animal Studies ◽

Meta Analysis ◽

Bone Matrix ◽

In Vivo Studies ◽

Defect Model ◽

Exclusion Criteria ◽

Freeze Dried ◽

Grafting Materials

The aim of this study is to systemically review the available evidence on the in vivo behavior of eggshell as a guided bone regeneration substitute material. Five databases (PubMed, Cochrane, Web of Science, Scopus, EMBASE) were searched up to October 2020. In vivo animal studies with a bone defect model using eggshell as a grafting material were included. Risk of bias was assessed using SYRCLE tool and the quality assessment using the ARRIVE guidelines. Overall, a total of 581 studies were included in the study, 187 after duplicate removal. Using the inclusion and exclusion criteria 167 records were further excluded. The full text of the remaining 20 articles was assessed for eligibility and included in the qualitative and quantitative assessment synthesis. There were different methods of obtaining eggshell grafting materials. Eggshell is a biocompatible grafting material, with osteoconduction proprieties. It forms new bone similar to Bio-Oss and demineralized freeze-dried bone matrix. It can be combined with other materials to enhance its proprieties. Due to the high variability of the procedures, animals, production and assessment methods, no meta-analysis could be performed. Eggshell might be considered a promising biomaterial to be used in bone grafting procedures, though further research is needed.

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Porous Silk Fibroin Membrane as a Potential Scaffold for Bone Regeneration

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.396-398.187 ◽

2008 ◽

Vol 396-398 ◽

pp. 187-190 ◽

Cited By ~ 5

Author(s):

Raquel Farias Weska ◽

Grínia M. Nogueira ◽

Wellington C. Vieira ◽

Marisa Masumi Beppu

Keyword(s):

Bone Regeneration ◽

Silk Fibroin ◽

Biomedical Application ◽

Early Stage ◽

Bone Matrix ◽

Octacalcium Phosphate ◽

Ion Concentration ◽

Phosphate Deposits

The requirements for scaffolds for bone tissue engineering include appropriate chemistry, morphology and structure to promote cell adhesion and synthesis of new bone matrix. Silk fibroin (SF) represents an important biomaterial for biomedical application, due to its suitable mechanical properties, biodegradability, biocompatibility, and versatility in processing. Our group has developed a new method to obtain a porous SF membrane, and the study of its potential for use as a scaffold for bone regeneration was the aim of this study. Porous membranes were obtained from SF solution, through the compression of a material generated by phase separation. For in vitro calcification experiments, porous SF membrane samples were immersed in SBF at pH 7.4 placed in polyethylene flasks. The experiments were carried out for seven days, at 36.5±0.5 °C. After 48 and 96h, the solutions were changed for fresh SBF with the ion concentration 1.5-fold higher than that of the standard one, to accelerate the calcification process. The characterization of morphology and composition of samples was performed by using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS), respectively. The SEM micrographs indicated that the porous SF membranes presented calcium phosphate deposits after undergoing in vitro calcification. These results were confirmed by EDS spectra, which showed a stoichiometric molar Ca/P ratio ranging from 1.27 to 1.52. This fact may suggest that calcification deposits consisted of mixtures of HAP (Ca/P ratio = 1.67) and transient HAP precursor phases, such as octacalcium phosphate (Ca/P = 1.33) and dicalcium phosphate dehydrate (Ca/P = 1), indicating early stage mineralization. The porous silk fibroin membrane analysed in the current study is a promising material to be used as scaffolds for bone regeneration.

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