Bovine Circadian Locomotor Output Cycles Kaput (CLOCK) and Clusterin (CLU) mRNA Quantitation in Ejaculated Crossbred Bull Spermatozoa

2015 ◽  
Vol 50 (3) ◽  
pp. 505-509 ◽  
Author(s):  
S Kumar ◽  
R Deb ◽  
U Singh ◽  
I Ganguly ◽  
DK Mandal ◽  
...  
Keyword(s):  
Mrna Quantitation

scholarly journals A beginner’s guide to RT-PCR, qPCR and RT-qPCR

2020 ◽  
Vol 42 (3) ◽  
pp. 48-53 ◽  
Author(s):  
Grace Adams
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
Life Science ◽  
Science Research ◽  
Quantitative Real Time Pcr ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Mrna Quantitation ◽  
Polymerase Chain

The development of the polymerase chain reaction (PCR), for which Kary Mullis received the 1992 Novel Prize in Chemistry, revolutionized molecular biology. At around the time that prize was awarded, research was being carried out by Russel Higuchi which led to the discovery that PCR can be monitored using fluorescent probes, facilitating quantitative real-time PCR (qPCR). In addition, the earlier discovery of reverse transcriptase (in 1970) laid the groundwork for the development of RT-PCR (used in molecular cloning). The latter can be coupled to qPCR, termed RT-qPCR, allowing analysis of gene expression through messenger RNA (mRNA) quantitation. These techniques and their applications have transformed life science research and clinical diagnosis.

scholarly journals Identification of differentially regulated genes in human patent ductus arteriosus

2016 ◽  
Vol 241 (18) ◽  
pp. 2112-2118 ◽  
Author(s):  
Pratik Parikh ◽  
Haiqing Bai ◽  
Michael F Swartz ◽  
George M Alfieris ◽  
David A Dean
Keyword(s):  
Cultured Cells ◽  
Ductus Arteriosus ◽  
Coarctation Of The Aorta ◽  
Transient Transfection ◽  
Differentially Expressed ◽  
Luciferase Reporter ◽  
Prostaglandin E ◽  
Promoter Regions ◽  
Mrna Quantitation ◽  
Multiple Patients

In order to identify differentially expressed genes that are specific to the ductus arteriosus, 18 candidate genes were evaluated in matched ductus arteriosus and aortic samples from infants with coarctation of the aorta. The cell specificity of the gene's promoters was assessed by performing transient transfection studies in primary cells derived from several patients. Segments of ductus arteriosus and aorta were isolated from infants requiring repair for coarctation of the aorta and used for mRNA quantitation and culturing of cells. Differences in expression were determined by quantitative PCR using the ΔΔCt method. Promoter regions of six of these genes were cloned into luciferase reporter plasmids for transient transfection studies in matched human ductus arteriosus and aorta cells. Transcription factor AP-2b and phospholipase A2 were significantly up-regulated in ductus arteriosus compared to aorta in whole tissues and cultured cells, respectively. In transient transfection experiments, Angiotensin II type 1 receptor and Prostaglandin E receptor 4 promoters consistently gave higher expression in matched ductus arteriosus versus aorta cells from multiple patients. Taken together, these results demonstrate that several genes are differentially expressed in ductus arteriosus and that their promoters may be used to drive ductus arteriosus-enriched transgene expression.

The effect of centrifugation on circulating mRNA quantitation opens up a new scenario in expression profiling from patients with metastatic colorectal cancer

2007 ◽  
Vol 40 (16-17) ◽  
pp. 1277-1284 ◽  
Author(s):  
S.C. Cesar Wong ◽  
Brigette B.Y. Ma ◽  
Paul B.S. Lai ◽  
Simon S.M. Ng ◽  
Janet F.Y. Lee ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Expression Profiling ◽  
Mrna Quantitation

Finding DNA regulatory motifs within unaligned noncoding sequences clustered by whole-genome mRNA quantitation

1998 ◽  
Vol 16 (10) ◽  
pp. 939-945 ◽  
Author(s):  
Frederick P. Roth ◽  
Jason D. Hughes ◽  
Preston W. Estep ◽  
George M. Church
Keyword(s):  
Whole Genome ◽  
Noncoding Sequences ◽  
Regulatory Motifs ◽  
Mrna Quantitation ◽  
Dna Regulatory Motifs

scholarly journals Tissue Factor mRNA Quantitation without Prior Monocyte Isolation

BioTechniques ◽  
2000 ◽  
Vol 28 (6) ◽  
pp. 1116-1124 ◽  
Author(s):  
Sandrine Javorschi ◽  
Sylvie Labrouche ◽  
Genviève Freyburger
Keyword(s):  
Tissue Factor ◽  
Mrna Quantitation ◽  
Monocyte Isolation

β-Glucuronidase is a suitable internal control gene for mRNA quantitation in pathophysiological and non-pathological livers

2013 ◽  
Vol 95 (2) ◽  
pp. 131-135 ◽  
Author(s):  
Hiromi Yamaguchi ◽  
Sawako Matsumoto ◽  
Mariko Ishibashi ◽  
Kiyoshi Hasegawa ◽  
Masahiko Sugitani ◽  
...  
Keyword(s):  
Internal Control ◽  
Control Gene ◽  
Internal Control Gene ◽  
Mrna Quantitation

Improved mRNA Quantitation in LightCycler RT-PCR

2003 ◽  
Vol 130 (1) ◽  
pp. 82-86 ◽  
Author(s):  
T.B. Ball ◽  
F.A. Plummer ◽  
K.T. HayGlass
Keyword(s):  
Rt Pcr ◽  
Mrna Quantitation
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