Relation between respiratory activity and sperm parameters in boar spermatozoa cryopreserved with alpha-tocopherol and selected by Sephadex

Objectives: Evaluate the effect of the packaging method in porcine semen cryopreserved with α-tocopherol on quality and functional sperm parameters. In porcine production, although the use of frozen semen is very limited, there are obvious advantages to use this technology. Material and Methods: Sperm samples were cryopreserved in pellets or straws with or without α-tocopherol and quality and functional parameters were determined in all groups. Results: As regards quality parameters, a significant individual effect was observed, with a similar behavior despite the packaging system evaluated. The same results were obtained in functional tests. Both packaging systems, pellets and straws, showed a similar behavior with respect to the effect of the antioxidant α-tocopherol on the quality and functional sperm parameters. Interestingly, the better results were obtained in pellets than in straws. Conclusion: The results obtained allow us to suppose that this efficient, economical and simple method, with little expensive equipment or supplies, can be used to cryopreserve boar spermatozoa for research. In fact, since the results have been better in tablets, if identification and storage problems of pellets were solved, this freezing method could be used for production purposes.

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Study of the protection of zinc and alpha-tocopherol on human sperm parameters, lipid peroxidation, apoptosis and DNA fragmentation with Mobile Phone Radiation treated in vitro

10.26226/morressier.573c1511d462b80296c98053 ◽

2016 ◽

Author(s):

Mohammad Hadi Bahadori

Keyword(s):

Lipid Peroxidation ◽

Mobile Phone ◽

Dna Fragmentation ◽

Human Sperm ◽

Alpha Tocopherol ◽

Sperm Parameters ◽

Mobile Phone Radiation

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THE METABOLISM OF STEROID HORMONES IN SEMEN

Journal of Endocrinology ◽

10.1677/joe.0.0300307 ◽

1964 ◽

Vol 30 (3) ◽

pp. 307-321 ◽

Cited By ~ 13

Author(s):

R. F. SEAMARK ◽

I. G. WHITE

Keyword(s):

Steroid Hormones ◽

Cetyltrimethylammonium Bromide ◽

Cold Shock ◽

Respiratory Activity ◽

Enzyme System ◽

Sperm Cells ◽

Cell Free Extract ◽

Deep Freezing ◽

A Cell ◽

Boar Spermatozoa

SUMMARY Testosterone is converted reversibly to androstenedione by the spermatozoa of certain mammals, such as the boar and stallion. The oxidation of testosterone to androstenedione occurs not only in the presence of fully active spermatozoa but also of sperm cells which have been deprived of their normal motility, and fructolytic as well as respiratory activity, by such treatment as cold-shock or deep-freezing. However, heating or treatment with certain inhibitors (e.g. cyanide and cetyltrimethylammonium bromide) renders the spermatozoa incapable of performing the testosterone ⇌ androstenedione reaction. The enzyme system required for that reaction has been obtained in the form of a cell-free extract from disintegrated boar spermatozoa. There is some evidence that it involves both NAD+ and NADP+. Oestradiol-17β is converted reversibly to oestrone. The reaction oestradiol → oestrone has been demonstrated in bull, boar and stallion semen, and the reverse reaction in boar and stallion semen. Progesterone is also metabolized in semen. The product may be 20β-hydroxy-pregn-4-en-3-one.

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Freeze-fracture observations on changes in the distribution of Filipin-sterol complexes during epididymal maturation and capacitation of boar spermatozoa

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157991 ◽

1990 ◽

Vol 48 (3) ◽

pp. 90-91

Author(s):

N. Seki ◽

Y. Toyama ◽

T. Nagano

Keyword(s):

Plasma Membrane ◽

Essential Role ◽

Freeze Fracture ◽

Final Concentration ◽

Freeze Fracturing ◽

Physiological Conditions ◽

Epididymal Maturation ◽

Cacodylate Buffer ◽

Sperm Membrane ◽

Boar Spermatozoa

It is believed that i ntramembra.nous sterols play an essential role in membrane stability and permeability. To investigate the distribution changes of sterols in sperm membrane during epididymal maturation and capacitation, filipin has been used as a cytochemical probe for the detection for membrane sterols. Using this technique in combination with freeze fracturing, we examined the boar spermatozoa under various physiological conditions.The spermatozoa were collected from: 1) caput, corpus and cauda epididymides, 2) sperm rich fraction of ejaculates, and 3)the uterus 2hr after natural coition. They were fixed with 2.5% glutaraldehyde in 0.05M cacodylate buffer (pH 7.4), and treated with the filipin solution (final concentration : 0.02.0.05%) for 24hr at 4°C with constant agitation. After the filipin treatment, replicas were made by conventional freeze-fracture technique. The density of filipin-sterol complexes (FSCs) was determined in the E face of the plasma membrane of head regions.

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Alpha-Tocopherol Protects Cultured Human Cells from the Acute Lethal Cytotoxicity of Dioxin

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.72.3.147 ◽

2002 ◽

Vol 72 (3) ◽

pp. 147-153 ◽

Cited By ~ 8

Author(s):

Kei-Ichi Hirai ◽

Jie-Hong Pan ◽

Ying-Bo Shui ◽

Eriko Simamura ◽

Hiroki Shimada ◽

...

Keyword(s):

Cell Death ◽

Cell Damage ◽

Smooth Endoplasmic Reticulum ◽

Dehydroascorbic Acid ◽

Human Cells ◽

Alpha Tocopherol ◽

Cervical Cancer Cells ◽

Cultured Human Cells ◽

Nuclear Damage ◽

Conjunctival Epithelial Cells

The possible protection of cultured human cells from acute dioxin injury by antioxidants was investigated. The most potent dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), caused vacuolization of the smooth endoplasmic reticulum and Golgi apparatus in cultured human conjunctival epithelial cells and cervical cancer cells. Subsequent nuclear damage included a deep irregular indentation resulting in cell death. A dosage of 30–40 ng/mL TCDD induced maximal intracellular production of H2O2 at 30 minutes and led to severe cell death (0–31% survival) at two hours. A dose of 1.7 mM alpha-tocopherol or 1 mM L-dehydroascorbic acid significantly protected human cells against acute TCDD injuries (78–97% survivals), but vitamin C did not provide this protection. These results indicate that accidental exposure to fatal doses of TCDD causes cytoplasmic free radical production within the smooth endoplasmic reticular systems, resulting in severe cytotoxicity, and that vitamin E and dehydroascorbic acid can protect against TCDD-induced cell damage.

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The Oral Intake of Organic Germanium, Ge-132, Elevates α-Tocopherol Levels in the Plas-ma and Modulates Hepatic Gene Expression Profiles to Promote Immune Activation in Mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000205 ◽

2014 ◽

Vol 84 (3-4) ◽

pp. 0183-0195 ◽

Cited By ~ 12

Author(s):

Takashi Nakamura ◽

Tomoya Takeda ◽

Yoshihiko Tokuji

Keyword(s):

Gene Expression ◽

Immune Activation ◽

Expression Profiles ◽

Water Soluble ◽

Alpha Tocopherol ◽

Hepatic Gene Expression ◽

Tocopherol Concentration ◽

Altered Expression ◽

Atp Production ◽

Transfer Protein

The common water-soluble organic germanium compound poly-trans-[(2-carboxyethyl) germasesquioxane] (Ge-132) exhibits activities related to immune responses and antioxidant induction. In this study, we evaluated the antioxidative effect of dietary Ge-132 in the plasma of mice. Male ICR mice (seven mice per group) received an AIN-76 diet with 0.05 % Ge-132; three groups received the Ge-132-containing diet for 0, 1 or 4 days. The plasma alpha-tocopherol (α-tocopherol) concentration increased from 6.85 to 9.60 μg/ml after 4 days of Ge-132 intake (p < 0.05). We evaluated the changes in hepatic gene expression related to antioxidative activity as well as in the entire expression profile after one day of Ge-132 intake, using DNA microarray technology. We identified 1,220 genes with altered expression levels greater than 1.5-fold (increased or decreased) as a result of Ge-132 intake, and α-tocopherol transfer protein (Ttpa) gene expression was increased 1.62-fold. Immune activation was identified as the category with the most changes (containing 60 Gene Ontology (GO) term biological processes (BPs), 41 genes) via functional clustering analysis of altered gene expression. Ge-132 affected genes in clusters related to ATP production (22 GO term BPs, 21 genes), lipid metabolism (4 GO term BPs, 38 genes) and apoptosis (5 GO term BPs). Many GO term BPs containing these categories were significantly affected by the Ge-132 intake. Oral Ge-132 intake may therefore have increased plasma α-tocopherol levels by up-regulating α-tocopherol transfer protein (Ttpa) gene expression.

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High Antibody Levels to Prothrombin Imply a Risk of Deep Venous Thrombosis and Pulmonary Embolism in Middle-aged Men

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657711 ◽

1997 ◽

Vol 78 (04) ◽

pp. 1178-1182 ◽

Cited By ~ 59

Author(s):

Timo Palosuo ◽

Jarmo Virtamo ◽

Jari Haukka ◽

Philip R Taylor ◽

Kimmo Aho ◽

...

Keyword(s):

Pulmonary Embolism ◽

Venous Thrombosis ◽

Deep Venous Thrombosis ◽

Plasma Proteins ◽

Alpha Tocopherol ◽

Index Number ◽

Thromboembolic Events ◽

Middle Aged ◽

Phospholipid Binding ◽

Antiprothrombin Antibodies

SummaryAntibodies against phospholipid-binding plasma proteins, such as β2-glycoprotein I (β2-GPI) and prothrombin, are associated with thromboembolic events in patients with systemic lupus erythematosus and also in subjects with no evident underlying diseases. We wanted to examine whether increased levels of antibodies to negatively-charged phospholipids (cardiolipin), to phospholipid-binding plasma proteins β2-GPI and prothrombin and to oxidised low-density lipoprotein (LDL) were associated with risk of deep venous thrombosis or pulmonary embolism in subjects with no previous thrombosis. The antibodies were measured in stored serum samples from 265 cases of deep venous thrombosis of the lower extremity or pulmonary embolism occurring during a median follow-up of about 7 years and from 265 individually matched controls. The study subjects were middle-aged men participating in a cancer prevention trial of alpha-tocopherol and beta-carotene and the cases of thromboembolic events were identified from nationwide Hospital Discharge Register.The risk for thrombotic events was significantly increased only in relation to antiprothrombin antibodies. As adjusted for body mass index, number of daily cigarettes and history of chronic bronchitis, myocardial infarction and heart failure at baseline, the odds ratio per one unit of antibody was 6.56 (95% confidence interval 1.73-25.0). The seven highest individual optical density-unit values of antiprothrombin antibodies were all confined to subjects with thromboembolic episodes.In conclusion, the present nested case-control study showed that high autoantibody levels against prothrombin implied a risk of deep venous thrombosis and pulmonary embolism and could be involved in the development of the thrombotic processes.

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