Morphological evaluation of Prochilodus lineatus embryos after vitrification–thawing in high‐osmolarity cryoprotectant solution

2018 ◽  
Vol 53 (6) ◽  
pp. 1353-1358
Author(s):  
Raphael da Silva Costa ◽  
Caio de Souza Capuzzo ◽  
Cristiele da Silva Ribeiro ◽  
Rosicleire Verissimo‐Silveira ◽  
Diógenes Henrique de Siqueira‐Silva ◽  
...  
Keyword(s):  
Prochilodus Lineatus ◽  
High Osmolarity ◽  
Morphological Evaluation ◽  
Cryoprotectant Solution

scholarly journals Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae)

2012 ◽  
Vol 10 (3) ◽  
pp. 661-665 ◽  
Author(s):  
Daniella A. J. Paula ◽  
Estefânia S. Andrade ◽  
Luis D. S. Murgas ◽  
Viviane O. Felizardo ◽  
Elissandra U. Winkaler ◽  
...  
Keyword(s):  
Vitamin E ◽  
Sodium Bicarbonate ◽  
Toxicity Test ◽  
Reduced Glutathione ◽  
Sodium Bicarbonate Solution ◽  
Distilled Water ◽  
Prochilodus Lineatus ◽  
Semen Cryopreservation ◽  
Cryoprotectant Solution

This study investigated the addition of antioxidants vitamin E and reduced glutathione on curimba (Prochilodus lineatus) semen cryopreservation and compared sodium bicarbonate solution and distilled water as activators. The experiment was conducted at the environmental station of CEMIG, in Itutinga-MG, Brazil, between December/2009 and January/2010. Semen samples (n = 7) with semen motility above 80% were diluted in cryoprotectant solutions composed of 10% methanol, 15% lactose and containing different concentrations of antioxidants: 50 (VE50), 100 (VE100) and 250 (VE250) µM of vitamin E, and 0.5 (RG0.5), 1.0 (RG1.0) and 1.5 (RG1.5) mM of reduced glutathione. A solution without antioxidants was used as a control. The semen was diluted at a ratio of 1:4 (100 ìL semen:400 ∝L cryoprotectant solution). The toxicity of the solutions was evaluated by investigating semen motility after 10 min in the solution. The rest of the diluted semen was placed into 0.5 mL straws maintained in nitrogen vapour for 24 hours and packed into a nitrogen liquid cylinder for four days. The samples were thawed in a water bath at 60°C for 8 s and the rate (%) and duration (s) of semen activation with distilled water or sodium bicarbonate was evaluated. In the toxicity test, we found that vitamin E and reduced glutathione were not toxic to curimba semen at any of the tested concentrations (P>0.05). The duration of motility was longer (P<0.05) in semen activated with sodium bicarbonate 1% (163 ± 11 s), which was considered the best activator for semen under these conditions. No significant differences were found between the cryoprotectant solutions used after freezing (P>0.05). Thus, the antioxidants vitamin E and reduced glutathione did not improve the quality of cryopreserved curimba semen, but they did not cause toxic effects to the semen in natura and they did not decrease its quality during cryopreservation.

Distribution of VLA-5 and VLA-4 fibronectin receptors in human monocytes demonstrated by immunofluorescence, immunocytochemistry, and autoradiography

1993 ◽  
Vol 51 ◽  
pp. 306-307
Author(s):  
Robert Williams ◽  
Che-Hung Lee ◽  
Sara E. Quella ◽  
David M. Harlan ◽  
Yuan-Hsu Kang
Keyword(s):  
Present Report ◽  
Matrix Proteins ◽  
Extracellular Matrices ◽  
Human Monocytes ◽  
Integrin Receptors ◽  
Morphological Evaluation ◽  
The Matrix ◽  
Specific Receptors ◽  
Cytokine Stimulation ◽  
Monocyte Adherence

Monocyte adherence to endothelial or extracellular matrices plays an important role in triggering monocyte activation in extravascular sites of infection, chronic inflammatory disorders, and tissue damage. Migration of monocytes in the tissues involves the response to a chemoattractant and movement by a series of attachments and detachments to the extracellular matrices which are regulated by expression and distribution of specific receptors for the matrix proteins such as fibronectin (FN). The VSAs (very late antigens or beta integrins), a subfamily of the transmembrane heterodimeric integrin receptors, have been thought to play a major role in monocyte adherence to the extracellular matrices and cells. In this subfamily, VLA-5 and VLA-4 are believed to be the most essential integrins mediating monocyte adherence to FN. In the present report, we have established and compared different procedures for morphological evaluation of the expression and distribution of the FN receptors on human monocytes in order to investigate their response to endotoxin or cytokine stimulation.

Ultrasound and clinical-morphological evaluation of fetal-placental complex of pregnant women with intrauterine infection

GYNECOLOGY ◽  
2015 ◽  
Vol 17 (5) ◽  
pp. 10-13
Author(s):  
S.M. Voevodin ◽  
◽  
T.V. Shemanaeva ◽  
A.I. Shchegolev ◽  
◽  
...  
Keyword(s):  
Pregnant Women ◽  
Intrauterine Infection ◽  
Morphological Evaluation

THE MORPHOLOGICAL EVALUATION OF IMMUNOGENICAL AND REACTOGENICAL PROPERTIES OF THE STRAIN «IK-4» OF THE CHICKEN INFECTIOUS ANAEMIA VIRUS

2018 ◽  
Vol 20 (5) ◽  
pp. 23-26
Author(s):  
I.N. Gromov ◽  
◽  
E.S. Kornyushina ◽  
A.S. Aliev ◽  
A.K. Alieva ◽  
...  
Keyword(s):  
Morphological Evaluation ◽  
Chicken Infectious Anaemia Virus

Acido delta-aminolevulínico dehidrasa (ALA-D) como biomarcador de exposición a plomo en peces

2008 ◽  
Author(s):  
◽  
Paula Edith Lombardi
Keyword(s):  
Cyprinus Carpio ◽  
Buenos Aires ◽  
Rio De La Plata ◽  
Río De La Plata ◽  
Prochilodus Lineatus ◽  
La Plata ◽  
Del Rio

La actividad de la enzima ácido δ-aminolevulínico dehidrasa (ALA-D) es un reconocido biomarcador específico de exposición a plomo. Debido a sus características ha sido utilizado en estudios de monitoreo ambiental para evaluar la exposición a plomo en diversos organismos, entre ellos peces. El principal objetivo del presente trabajo de tesis fue validar un método de evaluación general de riesgo ecotoxicológico por exposición a plomo en poblaciones naturales de peces. Para tal fin se seleccionaron tres especies recolectadas del río de la Plata: Prochilodus lineatus (sábalo), Leporinus obtusidens (boga) y Cyprinus carpio (carpa). Las zonas de muestreo elegidas fueron: Berazategui y Berisso ubicadas cerca de las emisiones de residuos cloacales provenientes de las ciudades de Buenos Aires y La Plata. La tercera, Atalaya, libre de emisiones cloacales, pero sujeta a emisiones diversas, todas ellas, de origen antropogénico. Los muestreos se realizaron entre junio de 2002 y mayo de 2004. En primera instancia se caracterizaron diversos parámetros de la enzima ALA-D en muestras de sangre y de tejido hepático. Los resultados obtenidos mostraron características similares en la enzima sanguínea de todas las especies estudiadas. Lo mismo se observó para la enzima hepática. La comparación entre tejidos mostró diferencias en el valor de Km, siendo menor para la enzima hepática. Posteriormente, se procedió a validar el uso de la enzima como parámetro biomarcador de exposición a plomo en estudios de campo.

Inter-Tissue and Intra-Tissue Co-Expression Networks in Human Metabolism: Morphological Evaluation of the Link Between Transcription Factors ERβ and NFAT in Morbid Obesity

2020 ◽  
Vol 17 (1) ◽  
pp. 63-80
Author(s):  
Athina Chasapi ◽  
Kostas Balampanis ◽  
Eleni Kourea ◽  
Fotios Kalfaretzos ◽  
Vaia Lambadiari ◽  
...  
Keyword(s):  
Morbid Obesity ◽  
Adipose Tissue ◽  
Clinicopathological Parameters ◽  
Morbidly Obese ◽  
Human Metabolism ◽  
Activated T Cells ◽  
Alcoholic Fatty Liver ◽  
Morphological Evaluation ◽  
Underlying Mechanisms

Background: Estrogen receptor β (ERβ) plays an important role in human metabolism and some of its metabolic actions are mediated by a positive “cross-talk” with Nuclear Factor of Activated T cells (NFAT) and the key metabolic transcriptional coregulator Transcriptional Intermediary Factor 2 (TIF2). Introduction: Our study is an “in situ” morphological evaluation of the communication between ERβ, NFAT and TIF2 in morbid obesity. Potential correlations with clinicopathological parameters and with the presence of diabetes and non-alcoholic fatty liver disease (NAFLD) were also explored. The aim of the present study was to determine the role of ERβ and NFAT in the underlying pathophysiology of obesity and related comorbidities. We have investigated the expression of specific proteins using immunochemistry methodologies. Methods: Our population consists of 50 morbidly obese patients undergoing planned bariatric surgery, during which biopsies were taken from visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), skeletal muscle (SM), extramyocellular adipose tissue (EMAT) and liver and the differential protein expression was evaluated by immunohistochemistry. Results: We demonstrated an extensive intra- and inter-tissue co-expression network, which confirms the tissue-specific and integral role of each one of the investigated proteins in morbid obesity. Moreover, a beneficial role of ERβ and NFATc1 against NAFLD is implicated, whereas the distinct roles of TIF2 still remain an enigma. Conclusions: We believe that our findings will shed light on the complex underlying mechanisms and that the investigated biomarkers could represent future targets for the prevention and therapy of obesity and its comorbidities.

scholarly journals Signal Transduction and Regulatory Mechanisms Involved in Control of the σS (RpoS) Subunit of RNA Polymerase

2002 ◽  
Vol 66 (3) ◽  
pp. 373-395 ◽  
Author(s):  
Regine Hengge-Aronis
Keyword(s):  
Rna Polymerase ◽  
Translational Control ◽  
Current Knowledge ◽  
Response Regulator ◽  
Regulatory System ◽  
Stress Conditions ◽  
Acidic Ph ◽  
High Osmolarity ◽  
Multiple Signal ◽  
Rpos Mrna

SUMMARY The σS (RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli and related bacteria. While rapidly growing cells contain very little σS, exposure to many different stress conditions results in rapid and strong σS induction. Consequently, transcription of numerous σS-dependent genes is activated, many of which encode gene products with stress-protective functions. Multiple signal integration in the control of the cellular σS level is achieved by rpoS transcriptional and translational control as well as by regulated σS proteolysis, with various stress conditions differentially affecting these levels of σS control. Thus, a reduced growth rate results in increased rpoS transcription whereas high osmolarity, low temperature, acidic pH, and some late-log-phase signals stimulate the translation of already present rpoS mRNA. In addition, carbon starvation, high osmolarity, acidic pH, and high temperature result in stabilization of σS, which, under nonstress conditions, is degraded with a half-life of one to several minutes. Important cis-regulatory determinants as well as trans-acting regulatory factors involved at all levels of σS regulation have been identified. rpoS translation is controlled by several proteins (Hfq and HU) and small regulatory RNAs that probably affect the secondary structure of rpoS mRNA. For σS proteolysis, the response regulator RssB is essential. RssB is a specific direct σS recognition factor, whose affinity for σS is modulated by phosphorylation of its receiver domain. RssB delivers σS to the ClpXP protease, where σS is unfolded and completely degraded. This review summarizes our current knowledge about the molecular functions and interactions of these components and tries to establish a framework for further research on the mode of multiple signal input into this complex regulatory system.

Ivermectin: A multilevel approach to evaluate effects in Prochilodus lineatus (Valenciennes, 1836) (Characiformes, Prochilodontidae), an inland fishery species

2021 ◽  
pp. 149515
Author(s):  
Ismael Esteban Lozano ◽  
Yanina Grisel Piazza ◽  
Paola Babay ◽  
Emanuel Sager ◽  
Fernando Román de la Torre ◽  
...  
Keyword(s):  
Prochilodus Lineatus ◽  
Multilevel Approach ◽  
Fishery Species

scholarly journals Reproducibility of CT-based radiomic features against image resampling and perturbations for tumour and healthy kidney in renal cancer patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Margherita Mottola ◽  
Stephan Ursprung ◽  
Leonardo Rundo ◽  
Lorena Escudero Sanchez ◽  
Tobias Klatte ◽  
...  
Keyword(s):  
Quantitative Imaging ◽  
Texture Features ◽  
Voxel Size ◽  
Morphological Evaluation ◽  
Resampling Procedure ◽  
Automatic Methods ◽  
Visual Assessments ◽  
2D And 3D ◽  
Slice Spacing ◽  
Original Information

AbstractComputed Tomography (CT) is widely used in oncology for morphological evaluation and diagnosis, commonly through visual assessments, often exploiting semi-automatic tools as well. Well-established automatic methods for quantitative imaging offer the opportunity to enrich the radiologist interpretation with a large number of radiomic features, which need to be highly reproducible to be used reliably in clinical practice. This study investigates feature reproducibility against noise, varying resolutions and segmentations (achieved by perturbing the regions of interest), in a CT dataset with heterogeneous voxel size of 98 renal cell carcinomas (RCCs) and 93 contralateral normal kidneys (CK). In particular, first order (FO) and second order texture features based on both 2D and 3D grey level co-occurrence matrices (GLCMs) were considered. Moreover, this study carries out a comparative analysis of three of the most commonly used interpolation methods, which need to be selected before any resampling procedure. Results showed that the Lanczos interpolation is the most effective at preserving original information in resampling, where the median slice resolution coupled with the native slice spacing allows the best reproducibility, with 94.6% and 87.7% of features, in RCC and CK, respectively. GLCMs show their maximum reproducibility when used at short distances.

scholarly journals Physiological performance of Kazachstania unispora in sourdough environments

2021 ◽  
Vol 37 (5) ◽  
Author(s):  
Dea Korcari ◽  
Giovanni Ricci ◽  
Claudia Capusoni ◽  
Maria Grazia Fortina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Environmental Factors ◽  
Positive Characteristic ◽  
Carbohydrate Source ◽  
High Osmolarity ◽  
Commercial Strain ◽  
Positive Properties ◽  
Nutritional Mutualism

AbstractIn this work we explored the potential of several strains of Kazachstania unispora to be used as non-conventional yeasts in sourdough fermentation. Properties such as carbohydrate source utilization, tolerance to different environmental factors and the performance in fermentation were evaluated. The K. unispora strains are characterized by rather restricted substrate utilization: only glucose and fructose supported the growth of the strains. However, the growth in presence of fructose was higher compared to a Saccharomyces cerevisiae commercial strain. Moreover, the inability to ferment maltose can be considered a positive characteristic in sourdoughs, where the yeasts can form a nutritional mutualism with maltose-positive Lactic Acid Bacteria. Tolerance assays showed that K. unispora strains are adapted to a sourdough environment: they were able to grow in conditions of high osmolarity, high acidity and in presence of organic acids, ethanol and salt. Finally, the performance in fermentation was comparable with the S. cerevisiae commercial strain. Moreover, the growth was more efficient, which is an advantage in obtaining the biomass in an industrial scale. Our data show that K. unispora strains have positive properties that should be explored further in bakery sector. Graphic abstract

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