Rosmarinic acid improves boar sperm quality, antioxidant capacity and energy metabolism at 17°C via AMPK activation

Abstract Background Cryopreservation induces transcriptomic and epigenetic modifications that strongly impairs sperm quality and function, and thus decrease reproductive performance. N6-methyladenosine (m6A) RNA methylation varies in response to stress and has been implicated in multiple important biological processes, including post-transcriptional fate of mRNA, metabolism, and apoptosis. This study aimed to explore whether cryopreservation induces m6A modification of mRNAs associated with sperm energy metabolism, cryoinjuries, and freezability. Results The mRNA and protein expression of m6A modification enzymes were significantly dysregulated in sperm after cryopreservation. Furthermore, m6A peaks were mainly enriched in coding regions and near stop codons with classical RRACH motifs. The mRNAs containing highly methylated m6A peaks (fts vs. fs) were significantly associated with metabolism and gene expression, while the genes with less methylated m6A peaks were primarily involved in processes regulating RNA metabolism and transcription. Furthermore, the joint analysis of DMMGs and differentially expressed genes indicated that both of these play a vital role in sperm energy metabolism and apoptosis. Conclusions Our study is the first to reveal the dynamic m6A modification of mRNAs in boar sperm during cryopreservation. These epigenetic modifications may affect mRNA expression and are closely related to sperm motility, apoptosis, and metabolism, which will provide novel insights into understanding of the cryoinjuries or freezability of boar sperm during cryopreservation.

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Boar sperm quality and oxidative status as affected by rosmarinic acid at 17 °C

Tropical Animal Health and Production ◽

10.1007/s11250-020-02246-1 ◽

2020 ◽

Vol 52 (4) ◽

pp. 2169-2177

Author(s):

Yulin He ◽

Dong Li ◽

Wenyu Zhang ◽

Xuekai Tian ◽

Weijun Pang ◽

...

Keyword(s):

Rosmarinic Acid ◽

Sperm Quality ◽

Oxidative Status ◽

Boar Sperm

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The effect of bee pollen addition diet and treadmill exercise on energy metabolism and antioxidant capacity in rats

Korean Journal of Sports Science ◽

10.35159/kjss.2018.02.27.1.977 ◽

2018 ◽

Vol 27 (1) ◽

pp. 977-987

Author(s):

Sam-Ja Noh ◽

Hyo-Bin Seo ◽

Tae-Dong Kwon

Keyword(s):

Energy Metabolism ◽

Antioxidant Capacity ◽

Treadmill Exercise ◽

Bee Pollen

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Effects of iodine methionine on boar sperm quality during liquid storage at 17°C

Reproduction in Domestic Animals ◽

10.1111/rda.13024 ◽

2017 ◽

Vol 52 (6) ◽

pp. 1061-1066 ◽

Cited By ~ 5

Author(s):

Q Fang ◽

J Wang ◽

YY Hao ◽

H Li ◽

JX Hu ◽

...

Keyword(s):

Sperm Quality ◽

Liquid Storage ◽

Boar Sperm

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Absence of seminal plasma from sperm-rich fraction decreases boar sperm quality characteristics during the course of liquid storage

Animal Reproduction Science ◽

10.1016/j.anireprosci.2018.08.029 ◽

2018 ◽

Vol 198 ◽

pp. 20-26 ◽

Cited By ~ 3

Author(s):

D.F. Leal ◽

M.A. Torres ◽

G.M. Ravagnani ◽

S.M.M.K. Martins ◽

F.V. Meirelles ◽

...

Keyword(s):

Seminal Plasma ◽

Sperm Quality ◽

Quality Characteristics ◽

Liquid Storage ◽

Boar Sperm

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In vitro effects of two different commercial freezing and thawing extenders on boar sperm quality

Animal Reproduction Science ◽

10.1016/j.anireprosci.2021.106906 ◽

2021 ◽

pp. 106906

Author(s):

De Andrade AFC ◽

R Grossfeld ◽

RV Knox

Keyword(s):

Sperm Quality ◽

Freezing And Thawing ◽

Boar Sperm ◽

In Vitro Effects

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Implication of Polyhistidine, a Novel Apoptosis Inhibitor, in Inhibiting Lipopolysaccharide-Induced Apoptosis in Boar Sperm

Animals ◽

10.3390/ani9100719 ◽

2019 ◽

Vol 9 (10) ◽

pp. 719 ◽

Cited By ~ 1

Author(s):

Tianzeng Song ◽

Yi Shi ◽

Yangang Wang ◽

Izhar Hyder Qazi ◽

Christiana Angel ◽

...

Keyword(s):

Sperm Quality ◽

Storage Conditions ◽

Potential Implication ◽

Liquid Storage ◽

Boar Sperm ◽

Tlr4 Agonist ◽

Reasonable Evidence ◽

Boar Semen ◽

Induced Apoptosis ◽

Apoptosis Inhibitor

Lipopolysaccharide (LPS) released from Gram-negative bacteria binds to toll-like receptor 4 (TLR4) and induces boar sperm apoptosis. Similarly, polyhistidine (pHis), a TLR4 agonist, can also bind to TLR4. We hypothesized that pHis could inhibit LPS-induced sperm apoptosis by competitively binding to TLR4 to then improve sperm quality. Therefore, the objective of this study was to examine whether pHis can inhibit LPS-induced sperm apoptosis and affect sperm quality. The results showed that the concentrations of bacterial colonies were significantly increased from 36 to 120 h under liquid storage conditions (p < 0.05); however, concentrations of LPS in boar semen showed a relatively constant trend (4.98 ± 1.55 EU/mL) following 120 h storage. The addition of 100 μg/mL pHis in the BTS extender significantly improved boar sperm motility and viability at 37 °C, and it significantly (p < 0.05) inhibited boar sperm apoptosis under liquid storage (17 °C) and at 37 °C incubation conditions. The co-treatment of LPS and pHis further confirmed that pHis played its role in inhibiting LPS-induced sperm apoptosis. In conclusion, our preliminary findings provide reasonable evidence that pHis could act as an inhibitor of LPS-induced apoptosis in boar sperm stored for longer periods of time. pHis might inhibit LPS-induced sperm apoptosis by competitively binding to TLR4. Nevertheless, further mechanistic studies are awaited to fully elucidate its potential implication in inhibiting LSP-induced apoptosis.

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Antioxidant Effect of a Polyphenol-Rich Murtilla (Ugni molinae Turcz.) Extract and Its Effect on the Regulation of Metabolism in Refrigerated Boar Sperm

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/2917513 ◽

2019 ◽

Vol 2019 ◽

pp. 1-15 ◽

Cited By ~ 3

Author(s):

Ignacio Jofré ◽

Magdalena Cuevas ◽

Leticia Signori de Castro ◽

João Diego de Agostini Losano ◽

Mariana Andrade Torres ◽

...

Keyword(s):

Lipid Peroxidation ◽

Sperm Motility ◽

Sperm Quality ◽

Membrane Damage ◽

Antioxidant Effect ◽

Boar Sperm ◽

Fertilization Capacity ◽

Ugni Molinae

The production of reactive oxygen species (ROS) in boar spermatozoa increases in refrigeration; this can have an impact on sperm quality and fertilization capacity. We evaluated the effect of polyphenol-rich aqueous extract of murtilla (Ugni molinae Turcz) on boar sperm stored at 17°C in order to reduce oxidative stress and improve sperm quality in the long term. Five experiments were performed: first, characterization of the polyphenol content from five genotypes of murtilla; second, determination of the genotype with the best antioxidant effect (MT-Ex); third, the antioxidant capacity on O2- and lipid peroxidation; fourth, the influence of MT-Ex on motility, calcium movement, cAMP, and metabolic parameters; and fifth, analysis of long-term refrigeration. The average phenolic content was 344 ppm; gallic acid, catechin, quercetin, myricetin, and kaempferol were detected. All extracts evaluated presented a concentration-dependent antioxidant effect. MT-Ex reduces intracellular O2-/peroxides but low lipid peroxidation. MT-Ex in nonstimulated ROS conditions reduces sperm motility, mitochondrial membrane potential, cAMP, and ATP, but the succinate dehydrogenase activity remained normal; also, we observed a reduction in calcium movement in in vitro sperm capacitation. The long-term analyses showed that MT-Ex improved sperm motility decay and reduced membrane damage and ROS at 168 h. Based on this study, we propose MT-Ex as a supplement in semen extenders.

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