In vitro effects of two different commercial freezing and thawing extenders on boar sperm quality

The production of reactive oxygen species (ROS) in boar spermatozoa increases in refrigeration; this can have an impact on sperm quality and fertilization capacity. We evaluated the effect of polyphenol-rich aqueous extract of murtilla (Ugni molinae Turcz) on boar sperm stored at 17°C in order to reduce oxidative stress and improve sperm quality in the long term. Five experiments were performed: first, characterization of the polyphenol content from five genotypes of murtilla; second, determination of the genotype with the best antioxidant effect (MT-Ex); third, the antioxidant capacity on O2- and lipid peroxidation; fourth, the influence of MT-Ex on motility, calcium movement, cAMP, and metabolic parameters; and fifth, analysis of long-term refrigeration. The average phenolic content was 344 ppm; gallic acid, catechin, quercetin, myricetin, and kaempferol were detected. All extracts evaluated presented a concentration-dependent antioxidant effect. MT-Ex reduces intracellular O2-/peroxides but low lipid peroxidation. MT-Ex in nonstimulated ROS conditions reduces sperm motility, mitochondrial membrane potential, cAMP, and ATP, but the succinate dehydrogenase activity remained normal; also, we observed a reduction in calcium movement in in vitro sperm capacitation. The long-term analyses showed that MT-Ex improved sperm motility decay and reduced membrane damage and ROS at 168 h. Based on this study, we propose MT-Ex as a supplement in semen extenders.

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Effects of Isatis root polysaccharide on boar sperm quality during liquid storage and in vitro fertilization

Animal Reproduction Science ◽

10.1016/j.anireprosci.2019.106178 ◽

2019 ◽

Vol 210 ◽

pp. 106178 ◽

Cited By ~ 1

Author(s):

Zhiqiang Ren ◽

Weike Shaoyong ◽

Qian Li ◽

Lu Ma ◽

Junying Xiao ◽

...

Keyword(s):

In Vitro Fertilization ◽

Sperm Quality ◽

Vitro Fertilization ◽

Liquid Storage ◽

Boar Sperm

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Effects of season, breed and age on the boar sperm quality: 2. The extended sperm

Biotechnology in Animal Husbandry ◽

10.2298/bah0304025s ◽

2003 ◽

Vol 19 (3-4) ◽

pp. 25-29

Author(s):

Blagoje Stancic ◽

Mladen Gagrcin ◽

Ivan Radovic

Keyword(s):

Sperm Quality ◽

Progressive Motility ◽

Boar Sperm ◽

June July

Total of 182 ejaculates were extended in 1:4 proportions and investigated for progressive motility (PM) ratio, after 24h and 72h of storage at +170C. The boars of Yorkshire, Landrace, Duroc, Hempshire and Pietrain pure breeds and F1-crossbreeds were divided according to age as: ?12, 13-18, 19-24 and ?25 months. Extended sperm samples were investigated within 4 seasons of year January-March, April-June, July-September and October-December. The PM ?65% was estimated in 60% of ejaculates after 24h and in 25% ejaculates after 72h preservation in vitro. Some differences in progressive motility, influenced by season, breed and age of boars, were established. Present results are preliminary and require further investigations.

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Effects of Atrazine and Fenoxaprop-Ethyl on Capacitation and the Acrosomal Reaction in Boar Sperm

International Journal of Toxicology ◽

10.1177/1091581809333138 ◽

2009 ◽

Vol 28 (1) ◽

pp. 24-32 ◽

Cited By ~ 13

Author(s):

Ramiro Maravilla-Galván ◽

Reyna Fierro ◽

Humberto González-Márquez ◽

Sandra Gómez-Arroyo ◽

Irma Jiménez ◽

...

Keyword(s):

Fatty Acids ◽

Plasma Membrane ◽

Human Health ◽

Endocrine Disruptor ◽

Acrosome Reaction ◽

Acrosomal Reaction ◽

Boar Sperm ◽

In Vitro Effects ◽

Membrane Destabilization

Atrazine is a herbicide of the chloro-s-triazine family. It inhibits photosynthesis in plants and is an endocrine disruptor, but its effects on human health are controversial. Fenoxaprop-ethyl, an aryloxy phenoxyalkanoic acid herbicide, inhibits the biosynthesis of fatty acids and provokes depolarization of membranes. The aim of this study is to evaluate the in vitro effects of both herbicides on capacitation, spontaneous acrosome reaction (SAR) and progesterone-induced acrosome reaction (PIAR) in boar sperm. Sperm capacitation is done in TALP-HEPES media for 4 hours. Capacitation and SAR are evaluated immediately; PIAR, 30 minutes later. LC50 for fenoxaprop-ethyl is 60 mM and 40 mM for atrazine. Fenoxaprop-ethyl induces capacitation at 60 mM and SAR at all concentrations, also increases significantly PIAR. Atrazine decreased capacitation whereas increase significantly SAR and PIAR at all concentrations. It seems that fenoxaprop-ethyl and atrazine accelerate the capacitation and the acrosomal reaction, possibly via plasma membrane destabilization.

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Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.2004.1369 ◽

2004 ◽

Vol 17 (10) ◽

pp. 1369-1373 ◽

Cited By ~ 2

Author(s):

C. S. Park ◽

M. Y. Kim ◽

Y. J. Yi ◽

Y. J. Chang ◽

S. H. Lee ◽

...

Keyword(s):

In Vitro Fertilization ◽

Sperm Quality ◽

Vitro Fertilization ◽

Boar Sperm

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Metformin improves boar sperm quality via 5′-AMP-activated protein kinase-mediated energy metabolism in vitro

动物学研究 ◽

10.24272/j.issn.2095-8137.2020.074 ◽

2020 ◽

Vol 41 (5) ◽

pp. 527-538

Author(s):

Rong-Nan Li ◽

◽

Zhen-Dong Zhu ◽

Yi Zheng ◽

...

Keyword(s):

Energy Metabolism ◽

Protein Kinase ◽

Sperm Quality ◽

Boar Sperm ◽

Amp Activated Protein Kinase

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IN VITRO EFFECTS OF RECOMBINANT TISSUE TYPE PLASMINOGEN ACTIVATOR ON FIBRINOLYTIC AND COAGULATION PARAMETERS AND ITS PREVENTION BY SPECIFIC ANTIBODY, D-PHE-PRO-ARG-CTUCl AND APROTININ

10.1055/s-0038-1643119 ◽

1987 ◽

Author(s):

E Seifried ◽

P Tanswell

Keyword(s):

Fibrinolytic Therapy ◽

Tissue Type ◽

Blood Collection ◽

Freezing And Thawing ◽

Blood Samples ◽

Hemostatic Factors ◽

In Vitro Effects ◽

Fibrinolytic Parameters

Monitoring of systemic effects during rt-PA therapy has shown of depletion of fibrinogen-antiplasmin, plasminogen and other hemostatic factors. Because in vitro activation of plasminogen may occur between blood collection and freezing and thawing before assaying we analysed the influence of 0,0.2, 2.0 and 10.0,ug rt-PA/ml citrate blood (final conc.) on hemostatic and fibrinolytic parameters and its inhibition by 3 different inhibitors. Addition of rt-PA to citrated whole blood without an inhibitor induced a concentration-dependent depletion of Fbg, Plgα2-Apl,α2-M, C1 - I, α2-Atrp, a loss of activity of FV, VIII,IX, XIII and alterations of the global coagulation assays. No effect of rt-PA was observed on F II, VII, X, XI, XII, AT III and Protein C. To prevent in vitro fibrinogenolysis 0.1, 0.5 and 1 mg/ml of a polyclonal sheep anti-rt-PA-antibody, 0.3, 1.0 and 10 Aimol/1 PPACK (D-Phe-Pro-Arg-CH2Cl), 75 and 150 KlU/ml aprotinin (final conc.) and saline as a control were added to pooled citrate blood.All samples containing rt-PA and/or inhibitors and/or saline were incubated for 45 min on ice, centrifuged, aliquotted, snap frozen and stored at ™20° C until analysis. Pretreatment of blood samples with anti-rt-PA IgG prevented interferences with all fibrinolytic and most clotting assays in plasma at a dose of 2 ,ug rt-PA/ ml. PPACK was of limited utility in clotting assays, but enabled correct analysis of fibrinolytic assays. Aprotinin was suitable only for a restricted range of both assay types. It is concluded that collection of blood samples on an appropriate antibody may be the most suitable procedure to get correct measurements of in-vivo effects of rt-PA on the hemostatic system in patients undergoing fibrinolytic therapy.

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Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Reproduction ◽

10.1530/rep-09-0274 ◽

2010 ◽

Vol 139 (3) ◽

pp. 557-564 ◽

Cited By ~ 8

Author(s):

María A Gil ◽

Marta Hernandez ◽

Jordi Roca ◽

Carmen Almiñana ◽

Xiomara Lucas ◽

...

Keyword(s):

Untreated Control ◽

Deleterious Effect ◽

Penetration Rate ◽

Sperm Quality ◽

Quality Parameters ◽

Final Concentration ◽

Freezing And Thawing ◽

Boar Spermatozoa

This study evaluated whether pentoxifylline added to freezing and thawing extenders influenced the function of boar spermatozoa. In Experiment 1, pooled ejaculated sperm-rich fractions were frozen in 0.5 ml straws after dilution in extender supplemented with pentoxifylline to a final concentration of 0, 2, 4, 8, 16 or 32 mM. The addition of 4, 8, 16 and 32 mM pentoxifylline to the freezing extender significantly decreased the progressive and total motility of spermatozoa. The percentage of viable spermatozoa with intact acrosomes as well as the penetration rate and the efficiency of fertilisation were significantly lower in pentoxifylline-treated groups compared with the untreated control. In Experiment 2, a pool of three straws with ‘good’ post-thaw sperm quality parameters and another three straws with ‘poor’ sperm quality were diluted in extender with 0, 1, 2, 4, 8, 16 or 32 mM pentoxifylline. Post-thaw samples with both ‘good’ and ‘poor’ sperm quality with 0, 2, 4, 8 and 16 mM were used to assess IVF parameters. The addition of pentoxifylline to post-thaw extender did not improve the post-thaw motility or viability of spermatozoa compared with the control. The in vitro penetration was higher (P<0.05) than the control for oocytes fertilised with spermatozoa that were thawed and incubated in extender with 4, 8 and 16 mM pentoxifylline. However, no differences were observed in the efficiency of fertilisation. We conclude that pentoxifylline, as a supplement added to the freezing extender, has a deleterious effect and that it does not improve the survival or in vitro fertilising efficiency of frozen–thawed boar spermatozoa when added after thawing.

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Effect of a pre-freezing treatment with cholesterol-loaded cyclodextrins on boar sperm longevity, capacitation dynamics, ability to adhere to porcine oviductal epithelial cells in vitro and DNA fragmentation dynamics

Reproduction Fertility and Development ◽

10.1071/rd12079 ◽

2013 ◽

Vol 25 (6) ◽

pp. 935 ◽

Cited By ~ 8

Author(s):

C. Tomás ◽

E. Blanch ◽

A. Fazeli ◽

E. Mocé

Keyword(s):

Dna Fragmentation ◽

Sperm Quality ◽

Epithelial Cell Line ◽

Dna Integrity ◽

Sperm Longevity ◽

Boar Sperm ◽

Fragmentation Dynamics ◽

Freezing Treatment

The aim of this work was to examine how a pre-freezing treatment with cholesterol-loaded cyclodextrins (CLC) affects boar sperm longevity, capacitation dynamics, ability to bind to a porcine telomerase-immortalised oviductal epithelial cell line (TERT-OPEC) in vitro and DNA integrity dynamics after freeze–thawing. Although the samples treated with CLC exhibited lower sperm quality than the control samples (P < 0.05) immediately after thawing, these differences disappeared (P > 0.05) after long-term incubation (26 h at 37 or 16°C). Additionally, the CLC-treated spermatozoa underwent similar capacitation and DNA fragmentation dynamics as the control spermatozoa (P > 0.05). However, CLC-treated spermatozoa were better able to bind to TERT-OPEC in vitro (P < 0.0001). In conclusion, the pre-freezing treatment of boar spermatozoa with CLC enhanced the ability of the spermatozoa to bind to TERT-OPEC in vitro, which could have an effect on the establishment of the sperm reservoir in the ampullary–isthmic junction in vivo. Additionally, frozen–thawed spermatozoa can be stored at 16°C for at least 6 h without a significant observable decline in sperm quality, which could be beneficial for the transport of thawed diluted doses of spermatozoa from the laboratory to the farm.

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Effect of L-carnitine on sperm quality during liquid storage of boar semen

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.19.0455 ◽

2020 ◽

Vol 33 (11) ◽

pp. 1763-1769

Author(s):

Kang Yang ◽

Na Wang ◽

Hai-Tao Guo ◽

Jing-Ran Wang ◽

Huan-Huan Sun ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Quality ◽

Membrane Integrity ◽

Quality Parameters ◽

Atp Content ◽

Developmental Potential ◽

Positive Effects ◽

Liquid Storage ◽

Boar Sperm

Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage.Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooledstorage at 17°C. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated.Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential.Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17°C.

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