Blood perfusion and diameter of bovine corpus luteum as predictors of luteal function in early pregnancy

2021 ◽  
Author(s):  
Gabriella dos Santos Velho ◽  
Monique Tomazele Rovani ◽  
Rogério Ferreira ◽  
Bernardo Garziera Gasperin ◽  
André Gustavo Cabrera Dalto
Keyword(s):  
Corpus Luteum ◽  
Early Pregnancy ◽  
Blood Perfusion ◽  
Luteal Function ◽  
Bovine Corpus Luteum

Actions of somatotrophin on oxytocin and progesterone release from the microdialysed bovine corpus luteum in vitro

1994 ◽  
Vol 143 (2) ◽  
pp. 243-250 ◽  
Author(s):  
J Liebermann ◽  
D Schams
Keyword(s):  
Early Pregnancy ◽  
Oestrous Cycle ◽  
Luteal Function ◽  
Bovine Corpus Luteum ◽  
Oxytocin Release ◽  
Dose Levels ◽  
Dose Dependent ◽  
Bovine Somatotrophin ◽  
Stimulation Of

Abstract In the present investigation, the effect of recombinant (BST) and pituitary-derived (bGH) bovine somatotrophin on progesterone and oxytocin release was examined. Individual copora lutea (CL) were obtained from cows at different stages of the oestrous cycle (days 5–7, 8–12 and 15–18) and also from early pregnancy (days 60–120) and were implanted with an in vitro microdialysis system (MDS). Perfusion with BST for 60 min (005, 0·5 and 5 μmol/l) induced a dose-dependent stimulation of progesterone release. Release of oxytocin from CL was significantly stimulated by BST at all dose levels. BST (0·5 μmol/l) stimulated progesterone release most during the early and mid-luteal phases and oxytocin release especially during the early luteal stage (days 5–7) of the oestrous cycle. CL from early pregnancy (days 60–120) treated with BST showed a significant response in progesterone and oxytocin release. bGH showed comparable effects. Our results suggest that somatotrophin acts directly on the secretory function of bovine CL in the MDS, specifically during the early luteal stage (days 5–7) of the oestrous cycle and early pregnancy (days 60–120). Somatotrophin may therefore have physiologically relevant effects associated with the development and maintenance of luteal function. Journal of Endocrinology (1994) 143, 243–250

scholarly journals Up-regulation of expression of interferon-stimulated gene 15 in the bovine corpus luteum during early pregnancy

2010 ◽  
Vol 93 (3) ◽  
pp. 1000-1011 ◽  
Author(s):  
L. Yang ◽  
X.L. Wang ◽  
P.C. Wan ◽  
L.Y. Zhang ◽  
Y. Wu ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Early Pregnancy ◽  
Regulation Of Expression ◽  
Bovine Corpus Luteum ◽  
Interferon Stimulated Gene 15

scholarly journals LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Reproduction ◽  
2016 ◽  
Vol 151 (4) ◽  
pp. 391-399 ◽  
Author(s):  
J Lüttgenau ◽  
K Herzog ◽  
K Strüve ◽  
S Latter ◽  
A Boos ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Cellular Localization ◽  
Mrna Levels ◽  
Toll Like Receptor 4 ◽  
Luteal Function ◽  
Mediated Effects ◽  
Bovine Corpus Luteum ◽  
Interleukin 1Α ◽  
Spatio Temporal ◽  
And Function

When given intravenously (iv), lipopolysaccharide (LPS) transiently suppresses the structure and function of the bovine corpus luteum (CL). This is associated with increased release of prostaglandin (PG) F2α metabolite. The underlying regulatory mechanisms of this process remain, however, obscure. Therefore, the aims of this study were: i) to investigate the expression of the LPS receptor toll-like receptor 4 (TLR4) and 2 (TLR2) in the bovine CL during early, mid- and late luteal phases; and ii) to further dissect the mechanisms of LPS-mediated suppression of luteal function. As revealed by semi-quantitative qPCR and immunohistochemistry, both receptors were detectable throughout the luteal lifespan. Their mRNA levels increased from the early toward the mid-luteal phase; no further changes were observed thereafter. The TLR4 protein seemed more highly represented than TLR2. The cellular localization of TLRs was in blood vessels; weaker signals were observed in luteal cells. Additionally, cows were treated either with LPS (iv, 0.5 μg/kg BW) or with saline on Day 10 after ovulation. Samples were collected 1200 h after treatment and on Day 10 of the respective subsequent (untreated) cycle. The mRNA expression of several possible regulatory factors was investigated, revealing the suppression of PGF2α receptor (PTGFR), STAR protein and 3β-hydroxysteroid dehydrogenase, compared with controls and subsequent cycles. The expression of TLR2 and TLR4, interleukin 1α (IL1A) and 1β (IL1B) and of PGF2α and PGE2 synthases (HSD20A and mPTGES respectively) was increased. The results demonstrate the presence of TLR2 and TLR4 in the bovine CL, and implicate their possible involvement in the deleterious effects of LPS on its function.

scholarly journals Genome-Wide Gene Expression Analysis Reveals a Dynamic Interplay between Luteotropic and Luteolytic Factors in the Regulation of Corpus Luteum Function in the Bonnet Monkey (Macaca radiata)

Endocrinology ◽  
2008 ◽  
Vol 150 (3) ◽  
pp. 1473-1484 ◽  
Author(s):  
S. Priyanka ◽  
P. Jayaram ◽  
R. Sridaran ◽  
R. Medhamurthy
Keyword(s):  
Gene Expression ◽  
Corpus Luteum ◽  
Signaling Pathways ◽  
Expression Analysis ◽  
Cross Talk ◽  
Early Pregnancy ◽  
Luteal Phase ◽  
Target Genes ◽  
Luteal Function ◽  
Genome Wide

Although LH is essential for survival and function of the corpus luteum (CL) in higher primates, luteolysis occurs during nonfertile cycles without a discernible decrease in circulating LH levels. Using genome-wide expression analysis, several experiments were performed to examine the processes of luteolysis and rescue of luteal function in monkeys. Induced luteolysis with GnRH receptor antagonist (Cetrorelix) resulted in differential regulation of 3949 genes, whereas replacement with exogenous LH (Cetrorelix plus LH) led to regulation of 4434 genes (1563 down-regulation and 2871 up-regulation). A model system for prostaglandin (PG) F2α-induced luteolysis in the monkey was standardized and demonstrated that PGF2α regulated expression of 2290 genes in the CL. Analysis of the LH-regulated luteal transcriptome revealed that 120 genes were regulated in an antagonistic fashion by PGF2α. Based on the microarray data, 25 genes were selected for validation by real-time RT-PCR analysis, and expression of these genes was also examined in the CL throughout the luteal phase and from monkeys treated with human chorionic gonadotropin (hCG) to mimic early pregnancy. The results indicated changes in expression of genes favorable to PGF2α action during the late to very late luteal phase, and expressions of many of these genes were regulated in an opposite manner by exogenous hCG treatment. Collectively, the findings suggest that curtailment of expression of downstream LH-target genes possibly through PGF2α action on the CL is among the mechanisms underlying cross talk between the luteotropic and luteolytic signaling pathways that result in the cessation of luteal function, but hCG is likely to abrogate the PGF2α-responsive gene expression changes resulting in luteal rescue crucial for the maintenance of early pregnancy. Results of genome-wide analyses suggest that curtailment of expression of LH target-genes through PGF2α action in corpus luteum involves cross talk between luteotropic and luteolytic signaling pathways.

scholarly journals Effects of oxytocin on the bovine corpus luteum of early pregnancy

Reproduction ◽  
1982 ◽  
Vol 66 (1) ◽  
pp. 75-78 ◽  
Author(s):  
G. J. S. Tan ◽  
R. Tweedale ◽  
J. S. G. Biggs
Keyword(s):  
Corpus Luteum ◽  
Early Pregnancy ◽  
Bovine Corpus Luteum

Uterine luteolytic hormone: a physiological role for prostaglandin F2alpha

1976 ◽  
Vol 56 (4) ◽  
pp. 595-651 ◽  
Author(s):  
E. W. Horton ◽  
N. L. Poyser
Keyword(s):  
Life Span ◽  
Corpus Luteum ◽  
Early Pregnancy ◽  
Physiological Role ◽  
Secretory Function ◽  
Prostaglandin F2alpha ◽  
Luteal Regression ◽  
Luteal Function ◽  
Gestational Period ◽  
Physiological Processes

To summarize luteal function during pregnancy briefly, there are physiological processes initiated by the embryo and/or conceptus in early pregnancy that serve to prolong the life-span of the corpus luteum. Some of these processes are well defined, but others remain more obscure. The corpus luteum is maintained in a functional state throughout pregnancy (at least in those species described in this review), even though in several species progesterone production by the corpus luteum is not required after the first third of the gestational period. The cessation of secretory function by the corpus luteum of pregnancy at the end of gestation is apparently actively induced. There is evidence in some species (especially the goat) that this is due to PGF2alpha released from the uterus or placenta. It is concluded that the occurrence of luteal regression in several species of mammal can be attributed to the physiological release of PGF2alpha from both the pregnant and nonpregnant uterus.

Effect of Exogenous Progesterone on the Bovine Corpus Luteum of Early Pregnancy

1961 ◽  
Vol 20 (1) ◽  
pp. 106-108 ◽  
Author(s):  
R. G. Zimbelman ◽  
R. G. Loy ◽  
L. E. Casida
Keyword(s):  
Corpus Luteum ◽  
Early Pregnancy ◽  
Bovine Corpus Luteum ◽  
Exogenous Progesterone

scholarly journals Downregulated luteolytic pathways in the transcriptome of early pregnancy bovine corpus luteum are mimicked by interferon-tau in vitro

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Raghavendra Basavaraja ◽  
Jessica N. Drum ◽  
Jackson Sapuleni ◽  
Lonice Bibi ◽  
Gilgi Friedlander ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Sonic Hedgehog ◽  
Early Pregnancy ◽  
Interferon Tau ◽  
Bovine Corpus Luteum ◽  
Canonical Pathways ◽  
Transcriptomic Signature ◽  
Distinct Features

Abstract Background Maintenance of the corpus luteum (CL) beyond the time of luteolysis is essential for establishing pregnancy. Identifying the distinct features of early pregnancy CL remains unresolved, hence we analyzed here the transcriptome of CL on day 18 pregnant (P) and non-pregnant (NP) cows using RNA-Seq. CL of P cows expressed ISGs, verifying exposure to the pregnancy recognition signal, interferon-tau (IFNT), whereas the CL of NP cows had elevated luteal progesterone levels, implying that luteolysis had not yet commenced. Results The DEGs, IPA, and metascape canonical pathways, along with GSEA analysis, differed markedly in the CL of P cows from those of NP cows, at the same day of the cycle. Both metascape and IPA identified similar significantly enriched pathways such as interferon alpha/beta, sonic hedgehog pathway, TNFA, EDN1, TGFB1, and PDGF. However, type-1 interferon and sonic hedgehog pathways were positively enriched whereas most of the enriched pathways were downregulated in the P compared to NP samples. Thirty-four % of these pathways are known to be elevated by PGF2A during luteolysis. Notably, selective DEGs in luteinized granulosa cells were modulated by IFNT in vitro in a similar manner to their regulation in the CL of P cows. Conclusion This study unraveled the unique transcriptomic signature of the IFNT-exposed, early pregnancy CL, highlighting the abundance of downregulated pathways known to be otherwise induced during luteolysis. These and IFNT-regulated in vitro pregnancy-specific DEGs suggest that IFNT contributes to the characteristics and maintenance of early pregnancy CL.

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