HighAN1variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves

2014 ◽  
Vol 80 (3) ◽  
pp. 527-540 ◽  
Author(s):  
Vincenzo D'Amelia ◽  
Riccardo Aversano ◽  
Giorgia Batelli ◽  
Immacolata Caruso ◽  
Mar Castellano Moreno ◽  
...  
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

scholarly journals The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhaofei Duan ◽  
Shiyu Tian ◽  
Guobin Yang ◽  
Min Wei ◽  
Jing Li ◽  
...  
Keyword(s):  
Plant Species ◽  
Anthocyanin Biosynthesis ◽  
Solanum Melongena ◽  
Amino Acid Sequences ◽  
Negative Regulator ◽  
Bimolecular Fluorescence Complementation ◽  
Luciferase Assay ◽  
Anthocyanin Accumulation ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

Many basic helix-loop-helix transcription factors (TFs) have been reported to promote anthocyanin biosynthesis in numerous plant species, but little is known about bHLH TFs that inhibit anthocyanin accumulation. In this study, SmbHLH1 from Solanum melongena was identified as a negative regulator of anthocyanin biosynthesis. However, SmbHLH1 showed high identity with SmTT8, which acts as a SmMYB113-dependent positive regulator of anthocyanin-biosynthesis in plants. Overexpression of SmbHLH1 in eggplant caused a dramatic decrease in anthocyanin accumulation. Only the amino acid sequences at the N and C termini of SmbHLH1 differed from the SmTT8 sequence. Expression analysis revealed that the expression pattern of SmbHLH1 was opposite to that of anthocyanin accumulation. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that SmbHLH1 could not interact with SmMYB113. Dual-luciferase assay demonstrated that SmbHLH1 directly repressed the expression of SmDFR and SmANS. Our results demonstrate that the biological function of bHLHs in anthocyanin biosynthesis may have evolved and provide new insight into the molecular functions of orthologous genes from different plant species.

scholarly journals Genome-wide analysis of basic helix–loop–helix superfamily members related to anthocyanin biosynthesis in eggplant (Solanum melongena L.)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7768 ◽  
Author(s):  
Shiyu Tian ◽  
Lujun Li ◽  
Min Wei ◽  
Fengjuan Yang
Keyword(s):  
Woody Plants ◽  
Tissue Specificity ◽  
Anthocyanin Biosynthesis ◽  
Solanum Melongena ◽  
Defense Responses ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Genome Wide ◽  
Solid Foundation ◽  
Functional Analyses

The basic helix–loop–helix (bHLH) superfamily is considered the second largest transcription factor (TF) family. It plays regulatory roles in the developmental processes of plants and in their defense responses. In recent years, many bHLH superfamily genes have been identified and characterized in herbaceous and woody plants. However, the comprehensive genomic and functional analyses of these genes in eggplant (Solanum melongena L.) have not been reported. In this study, 121 bHLH TFs were identified in the recently released eggplant genome. The phylogeny, gene structure and conserved motifs of the SmbHLH gene were comprehensively studied. Subsequently, the phylogenetic relationship between the bHLH of eggplant and the bHLH of other species was analyzed, and the proteins were classified into 17 subfamilies. Among these protein sequences, 16 subgroups were clustered into the functional clades of Arabidopsis. Two candidate genes (SmbHLH1, SmbHLH117) that may be involved in anthocyanin biosynthesis were screened. The tissue specificity or differential expression of the bHLH genes in different tissues and under various light and temperature conditions suggested the differential regulation of tissue development and metabolism. This study not only provides a solid foundation for the functional dissection of the eggplant bHLH gene family but may also be useful for the future synthesis of anthocyanins in eggplant.

Light-induced expression of basic helix-loop-helix genes involved in anthocyanin biosynthesis in flowers and leaves of Asiatic hybrid lily

2009 ◽  
Vol 121 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Akira Nakatsuka ◽  
Masumi Yamagishi ◽  
Michiharu Nakano ◽  
Keisuke Tasaki ◽  
Nobuo Kobayashi
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Induced Expression ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix
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