scholarly journals Arabidopsis replication factor C4 is critical for DNA replication during the mitotic cell cycle

2018 ◽  
Vol 94 (2) ◽  
pp. 288-303 ◽  
Author(s):  
Jie Qian ◽  
Yueyue Chen ◽  
Ying Hu ◽  
Yingtian Deng ◽  
Yang Liu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Mitotic Cell ◽  
Mitotic Cell Cycle ◽  
Replication Factor

scholarly journals A dependent pathway of gene functions leading to chromosome segregation in Saccharomyces cerevisiae.

1982 ◽  
Vol 94 (3) ◽  
pp. 718-726 ◽  
Author(s):  
J S Wood ◽  
L H Hartwell
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Chromosome Segregation ◽  
Nuclear Division ◽  
Mitotic Cell ◽  
Mitotic Cell Cycle ◽  
Gene Products ◽  
Dependent Pathway

Methyl-benzimidazole-2-ylcarbamate (MBC) inhibits the mitotic cell cycle of Saccharomyces cerevisiae at a stage subsequent to DNA synthesis and before the completion of nuclear division (Quinlan, R. A., C. I. Pogson, and K, Gull, 1980, J Cell Sci., 46: 341-352). The step in the cell cycle that is sensitive to MBC inhibition was ordered to reciprocal shift experiments with respect to the step catalyzed by cdc gene products. Execution of the CDC7 step is required for the initiation of DNA synthesis and for completion of the MBC-sensitive step. Results obtained with mutants (cdc2, 6, 8, 9, and 21) defective in DNA replication and with an inhibitor of DNA replication (hydroxyurea) suggest that some DNA replication required for execution of the MBC-sensitive step but that the completion of replication is not. Of particular interest were mutants (cdc5, 13, 14, 15, 16, 17, and 23) that arrest cell division after DNA replication but before nuclear division since previous experiments had not been able to resolve the pathway of events in this part of the cell cycle. Execution of the CDC17 step was found to be a prerequisite for execution of the MBC-sensitive step; the CDC13, 16 and 23 steps are executed independently of the MBC-sensitive step; execution of the MBC-sensitive step is prerequisite for execution of the MBC-sensitive step; execution of the MBC-sensitive step is prerequisite for execution of the CDC14 and 23 steps. These results considerably extend the dependent pathway of events that constitute the cell cycle of S. cerevisiae.

The regulation of the cell cycle during Drosophila embryogenesis: the transition to polyteny

Development ◽  
1991 ◽  
Vol 112 (4) ◽  
pp. 997-1008 ◽  
Author(s):  
A.V. Smith ◽  
T.L. Orr-Weaver
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Developmental Regulation ◽  
Mitotic Cell ◽  
Mitotic Cell Cycle ◽  
Drosophila Embryogenesis ◽  
First Instar ◽  
Late Embryogenesis ◽  
Gap Phase ◽  
Different Tissues

The process of polytenization plays a crucial role in Drosophila development, and most of the larval tissues are polytene. By analyzing the pattern of DNA replication in embryos pulse-labeled with BrdU, we show that many larval tissues undergo a transition to begin becoming polytene late in embryogenesis. Our results demonstrate that in these larval tissues polyteny results from a modified cell cycle, the endo cell cycle, in which there is only an S (synthesis) phase and a G (gap) phase. A key regulator of the mitotic cell cycle, the product of the string gene (the Drosophila homologue of cdc25), is not required for the endo cell cycle. The developmental regulation of the endo cell cycle is striking in that tissue-specific domains undergo polytene DNA replication in a dynamic pattern at defined times in embryogenesis. During subsequent rounds of the endo cell cycle in late embryogenesis and first instar larval development, the domains are subdivided and the temporal control is not as rigid. The length of the G phase varies among different tissues. By quantifying DNA content, we show that during the early polytene S phases the genome is not fully duplicated.

scholarly journals OsMre11 Is Required for Mitosis during Rice Growth and Development

2020 ◽  
Vol 22 (1) ◽  
pp. 169
Author(s):  
Miaomiao Shen ◽  
Yanshen Nie ◽  
Yueyue Chen ◽  
Xiufeng Zhang ◽  
Jie Zhao
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Genome Stability ◽  
Mitotic Cell ◽  
Mitotic Cell Cycle ◽  
Loss Of Function ◽  
Damage Repair ◽  
Dna Replication And Repair ◽  
Dna Strand ◽  
Vegetative And Reproductive Growth

Meiotic recombination 11 (Mre11) is a relatively conserved nuclease in various species. Mre11 plays important roles in meiosis and DNA damage repair in yeast, humans and Arabidopsis, but little research has been done on mitotic DNA replication and repair in rice. Here, it was found that Mre11 was an extensively expressed gene among the various tissues and organs of rice, and loss-of-function of Mre11 resulted in severe defects of vegetative and reproductive growth, including dwarf plants, abnormally developed male and female gametes, and completely abortive seeds. The decreased number of cells in the apical meristem and the appearance of chromosomal fragments and bridges during the mitotic cell cycle in rice mre11 mutant roots revealed an essential role of OsMre11. Further research showed that DNA replication was suppressed, and a large number of DNA strand breaks occurred during the mitotic cell cycle of rice mre11 mutants. The expression of OsMre11 was up-regulated with the treatment of hydroxyurea and methyl methanesulfonate. Moreover, OsMre11 could form a complex with OsRad50 and OsNbs1, and they might function together in non-homologous end joining and homologous recombination repair pathways. These results indicated that OsMre11 plays vital roles in DNA replication and damage repair of the mitotic cell cycle, which ensure the development and fertility of rice by maintaining genome stability.

scholarly journals Prevention of DNA Rereplication Through a Meiotic Recombination Checkpoint Response

2016 ◽  
Vol 6 (12) ◽  
pp. 3869-3881
Author(s):  
Nicole A Najor ◽  
Layne Weatherford ◽  
George S Brush
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Protein Kinase ◽  
Meiotic Recombination ◽  
Mitotic Cell ◽  
Mitotic Cell Cycle ◽  
Cyclin Dependent Kinase ◽  
Checkpoint Response ◽  
Recombination Checkpoint ◽  
Dna Rereplication

Abstract In the budding yeast Saccharomyces cerevisiae, unnatural stabilization of the cyclin-dependent kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks (DSBs) are generated but not repaired due to absence of DMC1, a pathway involving the checkpoint gene RAD17 prevents this DNA rereplication. Further genetic analysis has now revealed that prevention of DNA rereplication also requires MEC1, which encodes a protein kinase that serves as a central checkpoint regulator in several pathways including the meiotic recombination checkpoint response. Downstream of MEC1, MEK1 is required through its function to inhibit repair between sister chromatids. By contrast, meiotic recombination checkpoint effectors that regulate gene expression and cyclin-dependent kinase activity are not necessary. Phosphorylation of histone H2A, which is catalyzed by Mec1 and the related Tel1 protein kinase in response to DSBs, and can help coordinate activation of the Rad53 checkpoint protein kinase in the mitotic cell cycle, is required for the full checkpoint response. Phosphorylation sites that are targeted by Rad53 in a mitotic S phase checkpoint response are also involved, based on the behavior of cells containing mutations in the DBF4 and SLD3 DNA replication genes. However, RAD53 does not appear to be required, nor does RAD9, which encodes a mediator of Rad53, consistent with their lack of function in the recombination checkpoint pathway that prevents meiotic progression. While this response is similar to a checkpoint mechanism that inhibits initiation of DNA replication in the mitotic cell cycle, the evidence points to a new variation on DNA replication control.

scholarly journals Identification of genomic regions required for DNA replication during Drosophila embryogenesis.

Genetics ◽  
1993 ◽  
Vol 135 (3) ◽  
pp. 817-829 ◽  
Author(s):  
A V Smith ◽  
J A King ◽  
T L Orr-Weaver
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Mitotic Cell ◽  
Complementation Group ◽  
G1 Phase ◽  
Mitotic Cell Cycle ◽  
Drosophila Embryogenesis ◽  
Late Embryogenesis ◽  
Brdu Labeling ◽  
Genomic Regions

Abstract A collection of Drosophila deficiency stocks was examined by bromodeoxyuridine (BrdU) labeling of embryos to analyze the DNA replication patterns in late embryogenesis. This permitted us to screen 34% of the genome for genes that when absent in homozygous deficiencies affect the cell cycle or DNA replication. We found three genomic intervals that when deleted result in cessation of DNA replication in the embryo, 39D2-3;E2-F1, 51E and 75C5-7;F1. Embryos deleted for the 75C5-7;F1 region stop DNA replication at the time in embryogenesis when a G1 phase is added to the mitotic cell cycle and the larval tissues begin to become polytene. Thus, this interval may contain a gene controlling these cell cycle transitions. DNA replication arrests earlier in embryos homozygous for deletions for the other two regions. Analysis of the effects of deletions in the 39D2-3;E2-F1 region on DNA replication showed that the block to DNA replication correlates with deletion of the histone genes. We were able to identify a single, lethal complementation group in 51E, l(2)51Ec, that is responsible for the cessation of replication observed in this interval. Deficiencies that removed one of the Drosophila cdc2 genes and the cyclin A gene had no effect on replication during embryogenesis. Additionally, our analysis identified a gene, pimples, that is required for the proper completion of mitosis in the post-blastoderm divisions of the embryo.

scholarly journals The Role and Regulation of the preRC Component Cdc6 in the Initiation of Premeiotic DNA Replication

2004 ◽  
Vol 15 (5) ◽  
pp. 2230-2242 ◽  
Author(s):  
Yaara Ofir ◽  
Shira Sagee ◽  
Noga Guttmann-Raviv ◽  
Lilach Pnueli ◽  
Yona Kassir
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Chromatin Immunoprecipitation ◽  
Protein Complexes ◽  
Mitotic Cell ◽  
Preliminary Evidence ◽  
Mitotic Cell Cycle ◽  
The Stability ◽  
Meiotic Cycle

In all eukaryotes, the initiation of DNA replication is regulated by the ordered assembly of DNA/protein complexes on origins of DNA replication. In this report, we examine the role of Cdc6, a component of the prereplication complex, in the initiation of premeiotic DNA replication in budding yeast. We show that in the meiotic cycle, Cdc6 is required for DNA synthesis and sporulation. Moreover, similarly to the regulation in the mitotic cell cycle, Cdc6 is specifically degraded upon entry into the meiotic S phase. By contrast, chromatin-immunoprecipitation analysis reveals that the origin-bound Cdc6 is stable throughout the meiotic cycle. Preliminary evidence suggests that this protection reflects a change in chromatin structure that occurs in meiosis. Using the cdc28-degron allele, we show that depletion of Cdc28 leads to stabilization of Cdc6 in the mitotic cycle, but not in the meiotic cycle. We show physical association between Cdc6 and the meiosis-specific hCDK2 homolog Ime2. These results suggest that under meiotic conditions, Ime2, rather than Cdc28, regulates the stability of Cdc6. Chromatin-immunoprecipitation analysis reveals that similarly to the mitotic cell cycle, Mcm2 binds origins in G1 and meiotic S phases, and at the end of the second meiotic division, it is gradually removed from chromatin.

scholarly journals EFFECTS OF THE MITOTIC CELL-CYCLE MUTATION cdc4 ON YEAST MEIOSIS

Genetics ◽  
1977 ◽  
Vol 86 (1) ◽  
pp. 57-72
Author(s):  
G Simchen ◽  
J Hirschberg
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Nuclear Dna ◽  
Mitotic Cell ◽  
Restrictive Temperature ◽  
Mitotic Cell Cycle ◽  
Sporulation Medium ◽  
Permissive Temperature ◽  
Yeast Meiosis ◽  
High Degree

ABSTRACT The mitotic cell-cycle mutation cdc4 has been reported to block the initiation of nuclear DNA replication and the separation of spindle plaques after their replication. Meiosis in cdc4/cdc4 diploids is normal at the permissive temperature (25°) and is arrested at the first division (one-nucleus stage) at the restrictive temperature (34° or 36°). Arrested cells at 34° show a high degree of commitment to recombination (at least 50% of the controls) but no haploidization, while cells arrested at 36° are not committed to recombination. Meiotic cells arrested at 34° show a delayed and reduced synthesis of DNA (at most 40% of the control), at least half of which is probably mitochondrial. It is suggested that recombination commitment does not depend on the completion of nuclear premeiotic DNA replication in sporulation medium.—Transfer of cdc4/cdc4 cells to the restrictive temperature at the onset of sporulation produces a uniform phenotype of arrest at a 1-nucleus morphology. On the other hand, shifts of the meiotic cells to the restrictive temperature at later times produce two additional phenotypes of arrest, thus suggesting that the function of cdc4 is required at several points in meiosis (at least at three different times).

Myt1 Kinase Couples Mitotic Cell Cycle Exit with Differentiation in  Drosophila

2019 ◽  
Author(s):  
Reegan Josiah Willms ◽  
Jie Zeng ◽  
Shelagh D. Campbell
Keyword(s):  
Cell Cycle ◽  
Mitotic Cell ◽  
Mitotic Cell Cycle ◽  
Cell Cycle Exit

Paclitaxel inhibits osteoclast formation and bone resorption via influencing mitotic cell cycle arrest and RANKL-induced activation of NF-κB and ERK

2012 ◽  
Vol 113 (3) ◽  
pp. 946-955 ◽  
Author(s):  
Estabelle S. M. Ang ◽  
Nathan J. Pavlos ◽  
Shek Man Chim ◽  
Hao Tian Feng ◽  
Robin M. Scaife ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bone Resorption ◽  
Cell Cycle Arrest ◽  
Mitotic Cell ◽  
Osteoclast Formation ◽  
Mitotic Cell Cycle ◽  
Cycle Arrest
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