The kinetochore protein NNF1 has a moonlighting role in the vegetative development of Arabidopsis thaliana

2021 ◽  
Author(s):  
Allipra Sreejith ◽  
Krishnapriya Anirudhan ◽  
Siddharth Shivanandan ◽  
Abhishek Raghunathan ◽  
Ravi Maruthachalam
Keyword(s):  
Arabidopsis Thaliana ◽  
Vegetative Development ◽  
Kinetochore Protein

The Knockdown of Mitochondrial Uncoupling Proteins 1 and 2 (AtUCP1 and 2) in Arabidopsis thaliana Impacts Vegetative Development and Fertility

2021 ◽  
Author(s):  
Mariana de Lara Campos Arcuri ◽  
Alessandra Vasconcellos Nunes-Laitz ◽  
Rômulo Pedro Macêdo Lima ◽  
Pedro Barreto ◽  
Andressa Nagatani Marinho ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Expression Profiles ◽  
Primary Root ◽  
Uncoupling Proteins ◽  
Phenotypic Characterization ◽  
Respiratory Chain Complexes ◽  
Vegetative Development ◽  
Mitochondrial Uncoupling ◽  
Ros Homeostasis ◽  
Mitochondrial Uncoupling Proteins

Abstract Mitochondrial Uncoupling Proteins (UCPs) are mitochondrial inner membrane proteins that dissipate the proton electrochemical gradient generated by the respiratory chain complexes. In plants, these proteins are crucial for maintaining mitochondrial reactive oxygen species (ROS) homeostasis. In this study, single T-DNA insertion mutants for two (AtUCP1and AtUCP2) out of the three UCP genes present in Arabidopsis thaliana were employed to elucidate their potential roles in planta. Our data revealed a significant increase in the ATP/ADP ratios of both mutants, indicating clear alterations in energy metabolism, and a reduced respiratory rate in atucp2. Phenotypic characterization revealed that atucp1 and atucp2 plants displayed reduced primary root growth under normal and stressed conditions. Moreover, a reduced fertility phenotype was observed in both mutants, which exhibited increased number of sterile siliques and lower seed yield compared with wild-type plants. Reciprocal crosses demonstrated that both male and female fertility were compromised in atucp1, while such effect was exclusively observed in the male counterpart in atucp2. Most strikingly, a pronounced accumulation of hydrogen peroxide in the reproductive organs was observed in all mutant lines, indicating a disturbance in ROS homeostasis of mutant flowers. In line, the atucp1 and atucp2 mutants exhibited higher levels of ROS in pollen grains. Also in support, alternative oxidase 1a was highly induced in mutant flowers, while the expression profiles of transcription factors implicated in gene regulation during female and male reproductive organ/tissue development were perturbed. Overall, these data give support for an important role for AtUCP1 and AtUCP2 in flower oxidative homeostasis and overall plant fertility.

HASTY: a gene that regulates the timing of shoot maturation in Arabidopsis thaliana

Development ◽  
1998 ◽  
Vol 125 (10) ◽  
pp. 1889-1898 ◽  
Author(s):  
A. Telfer ◽  
R.S. Poethig
Keyword(s):  
Arabidopsis Thaliana ◽  
Flowering Time ◽  
Reproductive Development ◽  
Abaxial Surface ◽  
Vegetative Development ◽  
Shoot Morphology ◽  
Gibberellin Production ◽  
Number Of Leaves ◽  
Developmental Phases ◽  
Inappropriate Expression

In Arabidopsis thaliana, leaves produced at different stages of shoot development can be distinguished by the distribution of trichomes on the abaxial and adaxial surfaces. Leaves produced early in the development of the rosette (juvenile leaves) have trichomes on their adaxial, but not their abaxial surface, whereas leaves produced later in rosette development (adult leaves) have trichomes on both surfaces. In order to identify genes that regulate the transition between these developmental phases we screened for mutations that accelerate the production of leaves with abaxial trichomes. 9 alleles of the HASTY gene were recovered in this screen. In addition to accelerating the appearance of adult leaves these mutations also accelerate the loss of adaxial trichomes (a trait typical of bracts), reduce the total number of leaves produced by the shoot, and have a number of other effects on shoot morphology. The basis for this phenotype was examined by testing the interaction between hasty and genes that affect flowering time (35S::LEAFY, 35S::APETALA1, terminal flower1), gibberellin production (ga1-3) or perception (gai), and floral morphogenesis (leafy, apetala1, agamous). We found that hasty increased the reproductive competence of the shoot, and that its does not require gibberellin or a gibberellin response for its effect on vegetative or reproductive development. The phenotype of hasty is not suppressed by leafy, apetala1 and agamous, demonstrating that this phenotype does not result from the inappropriate expression of these genes. We suggest that HASTY promotes a juvenile pattern of vegetative development and inhibits flowering by reducing the competence of the shoot to respond to LEAFY and APETALA1.

scholarly journals A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development

2005 ◽  
Vol 56 (418) ◽  
pp. 2071-2083 ◽  
Author(s):  
José María Barrero ◽  
Pedro Piqueras ◽  
Miguel González-Guzmán ◽  
Ramón Serrano ◽  
Pedro L. Rodríguez ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Mutational Analysis ◽  
Vegetative Development

Characterization of the Germinal and Somatic Activity of the Arabidopsis Transposable Element Tag1

Genetics ◽  
1998 ◽  
Vol 148 (1) ◽  
pp. 445-456
Author(s):  
Dong Liu ◽  
Nigel M Crawford
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Flower Development ◽  
Copy Number ◽  
Lateral Roots ◽  
Reproductive Development ◽  
Vegetative Development ◽  
Male And Female ◽  
Late Stages

Abstract Tag1 is an autonomous transposon of Arabidopsis thaliana. The excision behavior of Tag1 during reproductive and vegetative development was examined using CaMV 35S-Tag1-GUS constructs. Germinal reversion frequencies varied from 0 to 27% and correlated with Tag1 copy number. Southern blot and somatic sector analyses indicated that each revertant was derived from an independent excision event, and approximately 75% of the revertants had new Tag1 insertions. Revertants were obtained with similar frequencies from the male and female parents. In flowers, small somatic sectors were observed in siliques, carpels, petals and sepals while stemlike organs (filaments and pedicels) had larger sectors. No sectors encompassing entire flowers or inflorescences were observed, however, indicating that excision occurs late in flower development and rarely in inflorescence meristems. Late excision was also observed during vegetative development with 99.8% of leaves showing small sectors encompassing no more than 20 cells. Roots and cotyledons, however, showed larger sectors that included entire lateral roots and cotyledons. These results indicate that Tag1 can excise in the embryo and all the organs of the plant with the timing of excision being restricted to late stages of vegetative and reproductive development in the shoot.

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

Localization of tandemly repeated DNA sequences in Arabidopsis thaliana

1991 ◽  
Vol 1 (2) ◽  
pp. 159-166 ◽  
Author(s):  
J. Maluszynska ◽  
J.S. Heslop-Harrison
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Sequences ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
Tandemly Repeated Dna

The effect of daylength on the transition to flowering in phytochrome-deficient, late-flowering and double mutants of Arabidopsis thaliana

1995 ◽  
Vol 95 (2) ◽  
pp. 260-266 ◽  
Author(s):  
Maarten Koornneef ◽  
Corrie Hanhart ◽  
Patty van Loenen-Martinet ◽  
Hetty Blankestijn de Vries
Keyword(s):  
Arabidopsis Thaliana ◽  
Late Flowering ◽  
Transition To Flowering

Control of carbohydrate metabolism in a starchless mutant of Arabidopsis thaliana

1992 ◽  
Vol 85 (3) ◽  
pp. 446-452
Author(s):  
Richard C. Sicher ◽  
Diane F. Kremer
Keyword(s):  
Arabidopsis Thaliana ◽  
Carbohydrate Metabolism ◽  
Starchless Mutant

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

Ammonium uptake and cellular alkalisation in roots of Arabidopsis thaliana: The involvement of cytoplasmic calcium

2000 ◽  
Vol 110 (4) ◽  
pp. 518 ◽  
Author(s):  
Christoph Plieth ◽  
Burkhard Sattelmacher ◽  
Marc R. Knight
Keyword(s):  
Arabidopsis Thaliana ◽  
Ammonium Uptake ◽  
Cytoplasmic Calcium
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