Juglone ameliorates skin wound healing by promoting skin cell migration through Rac1/Cdc42/PAK pathway

2016 ◽  
Vol 24 (5) ◽  
pp. 786-794 ◽  
Author(s):  
Hussain M. Wahedi ◽  
Yong U. Park ◽  
Eun-Yi Moon ◽  
Sun Y. Kim
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Skin Cell

Rapid epithelialisation of fetal wounds is associated with the early deposition of tenascin

1991 ◽  
Vol 99 (3) ◽  
pp. 583-586 ◽  
Author(s):  
D.J. Whitby ◽  
M.T. Longaker ◽  
M.R. Harrison ◽  
N.S. Adzick ◽  
M.W. Ferguson
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Temporal Distribution ◽  
Cell Types ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Early Appearance ◽  
Complex Process ◽  
Healing Wounds ◽  
Fetal Wound

Wound healing is a complex process involving the interaction of many cell types with the extracellular matrix (ECM). Fetal skin wound healing differs from that in the adult in that it occurs rapidly and without scar formation. The mechanisms underlying these differing processes may be related to the fetal environment, the stage of differentiation of the fetal cells or the ECM deposited in the wound. The spatial and temporal distribution of two components of the ECM, fibronectin and tenascin, were studied by immunostaining of cryosections from trunk wounds of fetal and adult sheep. Epithelialisation was complete earlier in the fetal wound than in the adult. The distribution of fibronectin was similar in fetal and adult wounds but tenascin was present earlier in the fetal wound. Fibronectin has several roles in wound healing including acting as a substratum for cell migration and as a mediator of cell adhesion through cell surface integrins. The attachment of fibroblasts to fibronectin is inhibited by tenascin and during development the appearance of tenascin in the ECM of migratory pathways correlates with the initiation of cell migration. Similarly, the appearance of tenascin in healing wounds may initiate cell migration. Tenascin was present in these wounds prior to cell migration and the rapid epithelialisation of fetal wounds may be due to the early appearance of tenascin in the wound.

Hyaluronan in skin wound healing: therapeutic applications

2020 ◽  
Vol 29 (12) ◽  
pp. 782-787
Author(s):  
Ian CC King ◽  
Parviz Sorooshian
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Clinical Practice ◽  
Inflammatory Response ◽  
Tissue Repair ◽  
Therapeutic Potential ◽  
Skin Wound ◽  
Clinical Applications ◽  
Skin Wound Healing ◽  
Proliferative Phase

Hyaluronan is a vital constituent in effective skin wound healing. This polysaccharide is ubiquitous throughout the human body and has functional significance for tissue repair and remodelling. The importance of hyaluronan in the proliferative phase of healing is diverse, impacting on cell migration, proliferation, modification of the inflammatory response and on angiogenesis. As such, it holds therapeutic potential for a variety of clinical applications that range from facilitating effective wound healing to burns management and scarring. This overview of the multifaceted roles of hyaluronan considers its current applications to clinical practice in plastic surgery as well as the latest advances in research.

scholarly journals A “traffic control” role for TGFβ3: orchestrating dermal and epidermal cell motility during wound healing

2006 ◽  
Vol 172 (7) ◽  
pp. 1093-1105 ◽  
Author(s):  
Balaji Bandyopadhyay ◽  
Jianhua Fan ◽  
Shengxi Guan ◽  
Yong Li ◽  
Mei Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Epidermal Cell ◽  
Traffic Control ◽  
Transforming Growth Factor ◽  
Skin Wound ◽  
Epidermal Cells ◽  
Skin Wound Healing ◽  
Tgfβ Receptor ◽  
Dermal Cells

Cell migration is a rate-limiting event in skin wound healing. In unwounded skin, cells are nourished by plasma. When skin is wounded, resident cells encounter serum for the first time. As the wound heals, the cells experience a transition of serum back to plasma. In this study, we report that human serum selectively promotes epidermal cell migration and halts dermal cell migration. In contrast, human plasma promotes dermal but not epidermal cell migration. The on-and-off switch is operated by transforming growth factor (TGF) β3 levels, which are undetectable in plasma and high in serum, and by TGFβ receptor (TβR) type II levels, which are low in epidermal cells and high in dermal cells. Depletion of TGFβ3 from serum converts serum to a plasmalike reagent. The addition of TGFβ3 to plasma converts it to a serumlike reagent. Down-regulation of TβRII in dermal cells or up-regulation of TβRII in epidermal cells reverses their migratory responses to serum and plasma, respectively. Therefore, the naturally occurring plasma→serum→plasma transition during wound healing orchestrates the orderly migration of dermal and epidermal cells.

scholarly journals Transforming Growth Factor α (TGFα)-Stimulated Secretion of HSP90α: Using the Receptor LRP-1/CD91 To Promote Human Skin Cell Migration against a TGFβ-Rich Environment during Wound Healing

2008 ◽  
Vol 28 (10) ◽  
pp. 3344-3358 ◽  
Author(s):  
Chieh-Fang Cheng ◽  
Jianhua Fan ◽  
Mark Fedesco ◽  
Shengxi Guan ◽  
Yong Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Ldl Receptor ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Transforming Growth Factor Α ◽  
Dermal Cell ◽  
Factor Α

ABSTRACT Jump-starting and subsequently maintaining epidermal and dermal cell migration are essential processes for skin wound healing. These events are often disrupted in nonhealing wounds, causing patient morbidity and even fatality. Currently available treatments are unsatisfactory. To identify novel wound-healing targets, we investigated secreted molecules from transforming growth factor α (TGFα)-stimulated human keratinoytes, which contained strong motogenic, but not mitogenic, activity. Protein purification allowed us to identify the heat shock protein 90α (hsp90α) as the factor fully responsible for the motogenic activity in keratinocyte secretion. TGFα causes rapid membrane translocation and subsequent secretion of hsp90α via the unconventional exosome pathway in the cells. Secreted hsp90α promotes both epidermal and dermal cell migration through the surface receptor LRP-1 (LDL receptor-related protein 1)/CD91. The promotility activity resides in the middle domain plus the charged sequence of hsp90α but is independent of the ATPase activity. Neutralizing the extracellular function of hsp90α blocks TGFα-induced keratinicyte migration. Most intriguingly, unlike the effects of canonical growth factors, the hsp90α signaling overrides the inhibition of TGFβ, an abundant inhibitor of dermal cell migration in skin wounds. This finding provides a long-sought answer to the question of how dermal cells migrate into the wound environment to build new connective tissues and blood vessels. Thus, secreted hsp90α is potentially a new agent for wound healing.

scholarly journals Tropomyosin Regulates Cell Migration during Skin Wound Healing

2013 ◽  
Vol 133 (5) ◽  
pp. 1330-1339 ◽  
Author(s):  
Justin G. Lees ◽  
Yu Wooi Ching ◽  
Damian H. Adams ◽  
Cuc T.T. Bach ◽  
Michael S. Samuel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

scholarly journals The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 457
Author(s):  
Andreu Blanquer ◽  
Jana Musilkova ◽  
Elena Filova ◽  
Johanka Taborska ◽  
Eduard Brynda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Chronic Wounds ◽  
Human Keratinocytes ◽  
Skin Wound ◽  
Platelet Lysate ◽  
Therapeutic Approaches ◽  
Skin Wound Healing ◽  
New Therapeutic Approaches ◽  
Proliferation And Differentiation

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

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