Lab-on-Chip Microfluidics System for Single Cell Mass Measurement: A Comprehensive Review

2014 ◽  
Vol 69 (8) ◽  
Author(s):  
Md. Habibur Rahman ◽  
Mohd Ridzuan Ahmad
Keyword(s):  
Single Cell ◽  
Measurement System ◽  
Single Cell Analysis ◽  
Mass Measurement ◽  
Cell Mass ◽  
Comprehensive Review ◽  
Lab On Chip ◽  
Mass Sensitivity ◽  
Cantilever Arrays ◽  
On Chip

Single cell mass (SCM) is one of the intrinsic properties of cell and is a vital part of single cell analysis (SCA). To date, a myriad numbers of works has been successfully reported for single cell mass measurement but the reported information are scattered, consequently a comprehensive review becomes mandatory to bring them together. Lab-on-chip microfluidics system integrated with micro-resonator provided an excellent platform to measure single cell mass directly (in presence of cells). On-chip microfluidics system like suspended micro channel resonator (SMR) was reported for non-adherent single yeast cell mass while ‘living cantilever arrays’ (LCA) was proposed to measure adherent HeLa cell mass. On the other hand, cantilever based resonant mass measurement system has non-uniform mass sensitivity; this issue has been overcome by pedestal mass measurement system (PMMS). PMMS has a unique geometrical structure that provided uniform mass sensitivity to the sensing surface. Moreover, we presented a comprehensive discussion of each of the available methods of SCM elaborating the sensing mechanism, geometry of the sensor and governing equations. It is hoped that, information presented in this comprehensive review paper will be a valuable source for the single cell mass analysers and biological researchers.  

Single Cell Mass Measurement Using Drag Force Inside Lab-on-Chip Microfluidics System

2015 ◽  
Vol 14 (8) ◽  
pp. 927-934 ◽  
Author(s):  
Md. Habibur Rahman ◽  
Mohd Ridzuan Ahmad ◽  
Masaru Takeuchi ◽  
Masahiro Nakajima ◽  
Yasuhisa Hasegawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Drag Force ◽  
Mass Measurement ◽  
Cell Mass ◽  
Lab On Chip ◽  
On Chip

Lab-on-chip microfluidic system for single cell mass measurement

2014 ◽  
Author(s):  
Md. Habibur Rahman ◽  
Ida Laila Ahmad ◽  
Mostafa SayahKarajy ◽  
Mohd Ridzuan Ahmad ◽  
Masahiro Nakajima ◽  
...  
Keyword(s):  
Single Cell ◽  
Mass Measurement ◽  
Cell Mass ◽  
Microfluidic System ◽  
Lab On Chip ◽  
On Chip

scholarly journals Massively parallel quantification of phenotypic heterogeneity in single cell drug responses

2020 ◽  
Author(s):  
Benjamin B. Yellen ◽  
Jon S. Zawistowski ◽  
Eric A. Czech ◽  
Caleb I. Sanford ◽  
Elliott D. SoRelle ◽  
...  
Keyword(s):  
Machine Learning ◽  
Acute Myeloid Leukemia ◽  
Single Cell ◽  
Myeloid Leukemia ◽  
Single Cell Analysis ◽  
Phenotypic Heterogeneity ◽  
Massively Parallel ◽  
Cell Analysis ◽  
On Chip ◽  
Acute Myeloid

AbstractSingle cell analysis tools have made significant advances in characterizing genomic heterogeneity, however tools for measuring phenotypic heterogeneity have lagged due to the increased difficulty of handling live biology. Here, we report a single cell phenotyping tool capable of measuring image-based clonal properties at scales approaching 100,000 clones per experiment. These advances are achieved by exploiting a novel flow regime in ladder microfluidic networks that, under appropriate conditions, yield a mathematically perfect cell trap. Machine learning and computer vision tools are used to control the imaging hardware and analyze the cellular phenotypic parameters within these images. Using this platform, we quantified the responses of tens of thousands of single cell-derived acute myeloid leukemia (AML) clones to targeted therapy, identifying rare resistance and morphological phenotypes at frequencies down to 0.05%. This approach can be extended to higher-level cellular architectures such as cell pairs and organoids and on-chip live-cell fluorescence assays.

(Invited) On-Chip Characterization of Microcapsules Using a Capacitive Sensor for Microencapsulation and Single-Cell Analysis Applications

2021 ◽  
Vol MA2021-01 (60) ◽  
pp. 1603-1603
Author(s):  
Sajjad Janfaza ◽  
Seyedehhamideh Razavi ◽  
Arash Dalili ◽  
Mina Hoorfar
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Capacitive Sensor ◽  
Cell Analysis ◽  
On Chip

scholarly journals Automatic and Selective Single Cell Manipulation in a Pressure-Driven Microfluidic Lab-On-Chip Device

Micromachines ◽  
2017 ◽  
Vol 8 (6) ◽  
pp. 172 ◽  
Author(s):  
Yigang Shen ◽  
Zhenyu Song ◽  
Yimo Yan ◽  
Yongxin Song ◽  
Xinxiang Pan ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Manipulation ◽  
Lab On Chip ◽  
On Chip ◽  
Single Cell Manipulation ◽  
Pressure Driven

Lab-on-chip device for single cell trapping and analysis

2013 ◽  
Vol 16 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Pratikkumar Shah ◽  
Xuena Zhu ◽  
Chunying Chen ◽  
Ye Hu ◽  
Chen-Zhong Li
Keyword(s):  
Single Cell ◽  
Lab On Chip ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
On Chip

scholarly journals Single-cell mass cytometry of microglia in major depressive disorder reveals a non-inflammatory phenotype with increased homeostatic marker expression

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chotima Böttcher ◽  
Camila Fernández-Zapata ◽  
Gijsje J. L. Snijders ◽  
Stephan Schlickeiser ◽  
Marjolein A. M. Sneeboer ◽  
...  
Keyword(s):  
Major Depressive Disorder ◽  
Depressive Disorder ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Mass ◽  
Brain Regions ◽  
Major Depressive ◽  
Immune Effector ◽  
Cytokines And Chemokines ◽  
The Brain

Abstract Stress-induced disturbances of brain homeostasis and neuroinflammation have been implicated in the pathophysiology of mood disorders. In major depressive disorder (MDD), elevated levels of proinflammatory cytokines and chemokines can be found in peripheral blood, but very little is known about the changes that occur directly in the brain. Microglia are the primary immune effector cells of the central nervous system and exquisitely sensitive to changes in the brain microenvironment. Here, we performed the first single-cell analysis of microglia from four different post-mortem brain regions (frontal lobe, temporal lobe, thalamus, and subventricular zone) of medicated individuals with MDD compared to controls. We found no evidence for the induction of inflammation-associated molecules, such as CD11b, CD45, CCL2, IL-1β, IL-6, TNF, MIP-1β (CCL4), IL-10, and even decreased expression of HLA-DR and CD68 in microglia from MDD cases. In contrast, we detected increased levels of the homeostatic proteins P2Y12 receptor, TMEM119 and CCR5 (CD195) in microglia from all brain regions of individuals with MDD. We also identified enrichment of non-inflammatory CD206hi macrophages in the brains of MDD cases. In sum, our results suggest enhanced homeostatic functions of microglia in MDD.

scholarly journals Integrated lab-on-chip biosensing systems based on magnetic particle actuation – a comprehensive review

Lab on a Chip ◽  
2014 ◽  
Vol 14 (12) ◽  
pp. 1966-1986 ◽  
Author(s):  
Alexander van Reenen ◽  
Arthur M. de Jong ◽  
Jaap M. J. den Toonder ◽  
Menno W. J. Prins
Keyword(s):  
Magnetic Fields ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Comprehensive Review ◽  
Lab On Chip ◽  
Diagnostic Assays ◽  
On Chip

A review on the use of magnetic particles that are actuated by magnetic fields for integrated lab-on-chip diagnostic assays.

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