scholarly journals Gas chromatographic analysis of fatty acid composition in the fresh water fishes in North Sulawesi

2018 ◽  
Vol 14 (1-2) ◽  
pp. 179-183
Author(s):  
Julius Pontoh ◽  
Drayen Tumiwa

Fresh water fish is one of the nutritious food sources for the people in Indonesia including in North Sulawesi.  Other than its protein, the fish oil has an important source of energy as well as nutraceutical of the people.  Some fatty acids, especially omega three fatty acids have been known for their function in prevention of some artery diseases.  There is not so much information available about the fatty acid composition in the fresh water fishes.  Therefore, the purposes of this research are to determine the present of fatty acids and their composition in the fresh water fishes.  The fish samples including, tilapia (mujair), carp (ikan mas), catfish (lele), snakehead (gabus) have been collected from various parts in North Sulawesi.  Fatty acids were identified using standard fatty acid mix and measured for their concentration using gas chromatographic technique.  The main fatty acids are following to their abundancy: oleic acid varies from 27.24 % for snakehead to 42.22 % for catfish; palmitic acid varies from 22.32 % for carp to 26.91 % for snakehead fish; and linolenic acid varies from 11.89 % for snakehead to 22.46 % for carp.  Among the fresh water fishes, the snakehead fish has a unique fatty acid composition, it has high concentration of docosahexaenoic acid (C22:6, DHA).

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Meike Stumpp ◽  
Reinhard Saborowski ◽  
Simon Jungblut ◽  
Hung-Chang Liu ◽  
Wilhelm Hagen

Abstract Background Trophic interactions are key processes, which determine the ecological function and performance of organisms. Many decapod crustaceans feed on plant material as a source for essential nutrients, e.g. polyunsaturated fatty acids. Strictly herbivorous feeding appears only occasionally in marine decapods but is common in land crabs. To verify food preferences and to establish trophic markers, we studied the lipid and fatty acid composition of the midgut glands of two marine crab species (Grapsus albolineatus and Percnon affine), one semi-terrestrial species (Orisarma intermedium, formerly Sesarmops intermedius), and one terrestrial species (Geothelphusa albogilva) from Taiwan. Results All species showed a wide span of total lipid levels ranging from 4 to 42% of the dry mass (%DM) in the marine P. affine and from 3 to 25%DM in the terrestrial G. albogilva. Triacylglycerols (TAG) were the major storage lipid compound. The fatty acids 16:0, 18:1(n-9), and 20:4(n-6) prevailed in all species. Essential fatty acids such as 20:4(n-6) originated from the diet. Terrestrial species also showed relatively high amounts of 18:2(n-6), which is a trophic marker for vascular plants. The fatty acid compositions of the four species allow to clearly distinguish between marine and terrestrial herbivorous feeding due to significantly different amounts of 16:0, 18:1(n-9), and 18:2(n-6). Conclusions Based on the fatty acid composition, marine/terrestrial herbivory indices were defined and compared with regard to their resolution and differentiating capacity. These indices can help to reveal trophic preferences of unexplored species, particularly in habitats of border regions like mangrove intertidal flats and estuaries.


2021 ◽  
Vol 38 (2) ◽  
pp. 211-218
Author(s):  
Nurgül Şen Özdemir ◽  
Ali Muzaffer Feyzioğlu ◽  
Fatma Caf

Seasonal changes of the lipid and fatty acid composition of Pleurobrachia pileus investigated monthly from March 2012 to February 2013. Average total lipid content was determined as percentage (%) and per individual (mg ind-1). It was highest in February (1.48 %; 3.55 mg ind-1). However, it was proportionally the lowest in April (0.40 %), and per individual in August (0.33 mg ind-1). Major fatty acids of P. pileus were identified as 16:0, 14:0, 11:1 n-9c, 20:5 n-3, and 22:6 n-3. P. pileus had on average 27.27 % ∑SFA, 25.04 % ∑MUFA and 47.63 % ∑PUFA content. EPA and DHA were the major fatty acids from PUFA. Seasonal changes of DHA were more obvious than EPA (p<0.05). Herbivore calanoid zooplankton trophic markers; 20:1 n-9 and DHA/EPA and herbivory trophic markers; EPA and DHA content were high in P. pileus fatty acids. It showed that herbivory fatty acids were taken by feeding from herbivory zooplankton and phytoplankton. Diet was an important factor in seasonal fatty acid changes of P. pileus. In addition, we revealed that P. pileus has a rich lipid content and fatty acid composition and plays an important role in the Southeastern Black Sea ecosystem functionalities between herbivory and carnivory species.


Author(s):  
Ying Cui ◽  
Ying Wu ◽  
Zhao Li Xu ◽  
Jing Zhang

The stable carbon and nitrogen isotopes and fatty acid composition of tapertail anchovy (Coilia mystus) at four migration stages collected around the Changjiang Estuary were analysed to investigate the variations in the trophic biomarkers during the fish migration. δ13 C and δ15N values of C. mystus ranged from −21.5 to −15.4‰ and from 6.9–15.8‰, respectively. Both δ13C and δ15N were enriched during migration. Polyunsaturated fatty acids were the dominant fatty acids and the major fatty acids found in C. mystus were C20:5n-3, C22:6n-3, C20:4n-6, C16:0, C18:0, C16:1n-7, C18:1(n-9, n-7) and C20:1 + C22:1. Significant changes among C. mystus at different migration stages were found both in the fatty acid composition and specific fatty acid concentration. Though the enrichment of stable isotopes may due to multiple factors (e.g. diet shift, environment and ontogeny), the dietary influence can be determined by the variation in fatty acid composition. Changes in the concentrations of benthic markers (C18:1n-7 and C20:4n-6) and pelagic markers (C18:1n-9 and C20:1 + C22:1) in C. mystus during the migration may suggest that benthic and pelagic food sources alternately dominated the anchovies' diet during different migration stages. It seems that application of multiple biomarkers in the trophic study of migratory fish will elevate the reliability of the analysis.


1958 ◽  
Vol 35 (10) ◽  
pp. 503-505 ◽  
Author(s):  
Peter B. Kelly ◽  
Raymond Reiser ◽  
Donald W. Hood

2021 ◽  
Vol 4 (1) ◽  
pp. 57
Author(s):  
Seyed Mehrshad Gaffari ◽  
Zahra Khoshnood

Fatty acid composition of fish is one of the key factors for the evaluation of fish nutritional value and it could be different based on fish species, habitat, nutrition and etc. The aim of the present study was to evaluate the fatty acid composition of five commercially important freshwater fish species taken from fisheries sites at Dez River, Iran. The fish samples included Heckel’s Orontes barbell, Luciobarbus pectoralis (Heckel, 1843), Chub, Squalius cephalus (Linnaeus, 1758), Common carp, Cyprinus carpio (Linnaeus, 1758), Brond-snout, Chondrostoma regium (Heckel, 1843) and Longspine scraper, Capoeta trutta (Heckel, 1843). The determination of fatty acid contents of the fish fillets were conducted by means of Gas chromatography. Results showed that Luciobarbus pectoralis fillets had the highest amount of fatty acids (43.49%). Cyprinus carpio had the highest amount of saturated fat (SAF) (29.43%) whereas the Luciobarbus pectoralis fillets had the highest MUFA (Monounsaturated fatty acid) (18.51%). The Squalius cephalus fillets possessed the highest amount of PUFA (polyunsaturated fatty acid) (1.87%). The Chondrostoma regium fillets revealed the highest n-6 (1.1%) while the Squalius cephalus fillets had the highest omega 3 (1.87%). The results of the present study showed that these freshwater fish are good sources of n-3 fatty acids, especially EPA and DHA, and could be considered as a valuable nutritional source.


1985 ◽  
Vol 54 (03) ◽  
pp. 563-569 ◽  
Author(s):  
M K Salo ◽  
E Vartiainen ◽  
P Puska ◽  
T Nikkari

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.


Author(s):  
Mohamed Haniff Hanafy Idris ◽  
Yanty Noorzianna Manaf ◽  
Mohd Nasir Mohd Desa ◽  
Amalia Mohd Hashim ◽  
Muhamad Shirwan Abdullah Sani ◽  
...  

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