scholarly journals Polymorphism of CSN3, Pit-1, LGB and its impact on milk performance traits at the Czech Fleckvieh and Holstein breed

Author(s):  
Ivan Manga ◽  
Jan Říha ◽  
Irena Vrtková

The effect of known CSN3, Pit-1 and LGB genes single nucleotide polymorphism (SNP) on milk performance traits (milk yield, protein, fat and lactose content, count of somatic cells) at the Czech Fleckvieh and the Holstein cattle breed was estimated. For more exact results, tested cows of each breed came from one source, and were selected into two groups according to their lactation. We tested around 100 animals at the first and 25 at the high (more than five) lactation of both breeds. Our attention was aimed particularly on combined genotypes of favourable alleles. We confirmed the positive tendention of the cheese yield valuable CSN3/BB with Pit-1/AA combined genotype for high milk production. By estimation of single gene effect, we found out significant association (P ≤ 0.01) between milk yield (kg) and Pit-1 genotypes at Holstein cows with the high lactation. This dependence was observed at the Czech Fleckvieh breed too, even it didn’t get conclusive value. Among all tested individuals, CSN3 genotypes significantly influenced milk yield (AA > BB, P ≤ 0.01), milk fat (BB > AA, P ≤ 0.01) and protein content (BB > AA, P ≤ 0.01). The genotypes AB and BB of the LGB gene at Holstein cows were joined with higher milk yield in selected cattle groups opposite to AA genotype (P ≤ 0.01, P ≤ 0.05). The influence of LGB on the somatic cell count in created cattle groups was not confirmed. Estimation of allele and genotype frequency according to lactation stage didn’t reveal higher differences at non of tested genes in both breeds.

2004 ◽  
Vol 47 (2) ◽  
pp. 193-202
Author(s):  
D. Bömkes ◽  
H. Hamann ◽  
O. Distl

Abstract. Title of the paper: Estimation of genetic parameters for test day records of milk performance traits in German Improved Fawn The objectives of this study were to estimate genetic parameters for milk performance traits of German Improved Fawn by using univariate und multivariate animal models. The analysis was based on 27,778 test day records of 1,848 German Improved Fawn with 3,574 lactation records distributed over 229 flocks in Lower Saxony, Saxony and Baden-Wuerttemberg. Milk records were sampled between 1988 and 2002. The animals in our analysis were the progeny of 455 sires and 1.148 does. Heritabilities estimated with a multivariate test day model with fixed regression were h2 = 0.19, 0.16 and 0.15 for milk, fat and protein yield. For fat and protein content and Somatic Cell Score (SCS) heritabilities were h2 = 0.17, 0.14 and 0.16, respectively. The additive genetic correlations between milk yield and fat as well as protein yield of German Improved Fawn were very high and positive (rg = 0.84 and rg = 0.77). Milk yield and milk contents were genetically negative correlated with rg = −0.28 for fat and rg = −0.22 for protein content. A moderate additive genetic correlation (rg = 0.48) between fat and protein content was estimated. There were no considerable additive genetic correlations between fat yield and protein content as well as between fat content and protein yield (rg = 0.05 and rg = 0.09). Additive genetic correlations between milk, fat or protein yield and SCS were high and negative, whereas additive genetic correlations between fat or protein content and SCS were low and positive. The genetic parameters estimated from field test records allow to achieve genetic progress in milk performance traits of German Improved Fawn.


Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Radica Djedovic ◽  
Vladan Bogdanovic ◽  
Predrag Perisic ◽  
Dragan Stanojevic ◽  
Jelena Popovic ◽  
...  

The quality and yield of milk, and therefore of dairy products as well, depends directly on their protein composition. Milk gene proteins represent structural locuses that are functionally associated with milk production. In cattle selection a special importance is attached to the variability of kappa casein (?-CN) as one of the four casein milk proteins. Genotypes of 21 Simmental breed cows, 20 crossbred cows obtained by crossing of Simmental and Red Holstein breed, and 25 cows of autochthonous Busha breed were determined for kappa casein by restriction fragment length polymorphism analysis (PCR- RFLP) of amplified DNA. The ?-CN genotype frequencies for Simmental breed were: 42.8; 47.6 and 9.6 % for AA, and BB genotype, for crossbreds: 75.0; 25.0 and 0.0% and for Busha individuals: 41.7; 50.0 and 8.3 %, respectively. Frequencies of A and B alleles, assessed on the basis of genotypic frequencies were 0.667 and 0.333 for Simmental breed, 0.875 and 0.125 for crossbreds and 0.667 and 0.333 for autochthonous Busha cattle breed, respectively. For confirming the phenotypic variability of milk yield in standard lactation, yield of milk fat and the content of milk fat per ?-CN genotypes within breeds observed the fixed model which included the breed effect, lactation in order and effect of ?-CN genotype (AA, and BB) was used. The ?-CN genotype statistically significantly (p ?0.05) influenced milk yield and highly significantly (p ?0.01) milk fat yield, while the content of milk fat was not statistically significantly (p>0.05) influenced in examined animals.


2019 ◽  
Vol 59 (4) ◽  
pp. 749
Author(s):  
A. M. Zanine ◽  
G. P. R. Motta ◽  
D. J. Ferreira ◽  
A. L. de Souza ◽  
M. D. Ribeiro ◽  
...  

This study aimed to evaluate the effects of pasture allowances on forage production, milk yield and composition, and grazing behaviour of Holstein cows during autumn. Thirty-six Holstein cows calving in the autumn were allocated to blocks considering: calving date, bodyweight, and body score. Treatments consisted of three pasture allowances as follows: high (HA; 38.4 kg DM/cow.day), medium (MA; 30.3 kg DM/cow.day), and low (LA; 26.8 kg DM/cow.day) pasture allowances. Forage mass and forage height pre-grazing were similar (P > 0.05) for all grazing targets. Pasture-use efficiency was affected (P < 0.05) by the pasture allowance. The highest and lowest efficiency was obtained with treatments LA and HA, respectively. The neutral detergent fibre content of forage harvesting was affected (P < 0.05) by the pasture allowance, with average of 44.8, 47.0, and 49.4 to HA, MA, and LA, respectively. There was an effect of pasture allowances (P < 0.05) on the milk yield per hectare and milk yield per cow. The highest production was observed in LA (438 L/ha.day) and the lowest in HA (314 L/ha.day). The percentage of milk fat, milk protein, and milk fat (g/cow.day) did not differ (P > 0.05) among pasture allowances. There was effect (P < 0.05) of pasture allowance with higher grazing time (369 min) and lower rumination time (23.3 min) observed to HA. Pasture allowances affected (P < 0.05) the total number of bites/day and bites/min, but did not affect (P > 0.05) bite mass. The pasture managements with low (26.8 kg DM/cow.day) pasture allowances provided better conditions for milk yield per area, likely due to the better grazing efficiency. However, pasture allowance provide little measurable changes on grazing behaviour.


Author(s):  
A. Zheltikov ◽  
N. Kostomakhin ◽  
D. Adushinov ◽  
O. Zaiko ◽  
V. Dementev ◽  
...  

The characteristic of milk productivity of cows of Holstein and Simmental breeds in LLC “Sibirskaya Niva” in the Maslyaninsky district in the Novosibirsk region has been given. It has been revealed that under the natural and climatic environments of the Western Siberia, when creating favorable conditions for feeding and housing for cows of Holstein and Simmental breeds have sufficiently high milk productivity. It has been found that Holstein first-calf heifers surpassed their Simmental herdmates by 959 kg in milk yield for the first 305 days of lactation under the same conditions of housing and feeding, by 3,1 kg in daily milk yield, by 38,2 kg in milk fat yield, and by 28,3 kg in milk protein yield (P < 0,001). There were no statistically significant differences between animals of Holstein and Simmental breeds in terms of fat and protein content in milk, which amounted to 3,94 and 2,96 % in Holstein breed and 3,97 and 2,98 % in Simmental breed, respectively. Both breeds have shown high milk productivity during the first 3 lactations. Thus, the milk yield of Holstein cows for the first 3 lactation was 6475–9290 kg, Simmental cows it was 5516–7680 kg, fat content was 3,93–3,99 and 3,96–3,97 %, respectively. The protein content in the milk of cows of both breeds did not exceed 3,0 %. High variability has been found in milk yield, yield of milk fat and protein, the coefficient of milk content and duration of lactation, the lowest in the content of protein and fat in milk and live weight. The correlation coefficients between the fat and protein content in milk were generally positive, but did not exceed 0,28. Therefore, breeding in the herd of cows in LLC “Sibirskaya Niva” have to be conducted not only by fat content, but also by protein content in milk.


2011 ◽  
Vol 27 (3) ◽  
pp. 951-958 ◽  
Author(s):  
D. Kucevic ◽  
S. Trivunovic ◽  
M. Radinovic ◽  
M. Plavsic ◽  
Z. Skalicki ◽  
...  

Objective of this study was to analyze the effect of the dairy farm on milk traits of cows in Vojvodina. The research was carried out on small farms with 10 to 20 cows, medium farms with 20 to 50 cows, and large farms with over 50 dairy cows. The study included registered animals of Simmental (SM) and Holstein-Friesian breed (HF; including Red Holstein) in the first lactation for traits of milk yield and yield and content of milk fat. Total of 1323 first lactations were analyzed. The average milk yield (both breeds) in the first lactation of 305 days was 6295 kg of milk with 234,3 kg of milk fat and average milk fat content of 3,74%. Milk performance of cows varied significantly (CV=22,9% and SD=1447,8), as well as milk fat yield (CV=21,6% and SD=50,8). Large farms produced in average 6534 kg of milk, medium farms 6347kg and small farms 4717kg. Size of the farm exhibited significantly high effect on all observed traits, and the tendency was that farms with higher number of animals realize also higher average of production. Farm management and various breeding-zootechnical conditions present on farms had significant effect on milk performance of cows.


2020 ◽  
Vol 36 (4) ◽  
pp. 417-426
Author(s):  
Dragan Niksic ◽  
Vlada Pantelic ◽  
Dusica Ostojic-Andric ◽  
Predrag Perisic ◽  
Ljiljana Samolovac ◽  
...  

In order to examine the variability of production performance traits (lactation duration, milk yield for the whole lactation, milk yield in standard lactation, milk fat content, milk fat yield in standard lactation, protein content and yield in standard lactation), 954 cows, domestic and imported Simmental populations, were included in the study, with a total of 3641 completed lactations. Cows were located in the area of Toplica district, reared on individual farming households (tied system) and on the farm with intensive farming (free system). Based on the rearing method and origin, animals were divided into four groups: Group 1 (domestic animals reared by individual agricultural producers on individual farming households); Group 2 (imported animals reared by individual agricultural producers on individual farming households); Group 3 (domestic animals reared on the farm) and Group 4 (imported animals reared on the farm). The fourth group of studied cows showed the highest yield and protein content, while the cows of the third group had the longest lactation and the highest milk fat content. All production performance traits varied highly significantly (p?0.001) under the influence of the combined factor of housing/rearing method and origin.


Author(s):  
N. Kosyachenko ◽  
Marina Abramova ◽  
M. Lapina

Abstract. Aim. The study of the influence of crossbriding on the growth and development of heifers and subsequent productive and reproductive qualities of cows on the first lactation. Methods. Using statistical methods, correlation analysis and ANOVA for evaluated genetic indicators of economic-valuable traits of dairy cattle. In particular was studied live weight of heifers from birth to 18 months, first insemination age and first fecundation age, live weight at fecundation, frequency of insemination, first calving age, days open, live weight at first lactation, milk yield for 305 days (kg), fat (%, kg) and protein (%, kg) in milk. Results. Relationship between productive indicators and the genotype of the animal is established. When assessing the strength of the influence of the factor «blood on the Holstein breed» it was found that the greatest impact can be traced at the age of 6 and 18 months, and is respectively 12.7 % and 17.1 %.The reproductive qualities of the first-calf cows decreased with the increase percent of blood in the Holstein breed. Evaluation of the impact of crossbreeding on milk production at first lactation has revealed significant strong influence on milk yield for 305 days of first lactation, and related signs the amount of milk fat and protein. According to qualitative indicators, the low influence of the Holstein blood fraction was found, while to a greater extent on the fat content than on the protein content in milk. Evaluation of phenotypic and genotypic correlations of milk yield and quality components of milk revealed average negative phenotypic and low negative genotypic correlation in all groups. The exception was in heifers of Yaroslavl breed with percent of Holstein blood less than 50 %, in which the relationship of yield x protein was positive. The relationship between milk yield and fat and protein content in milk has the opposite moderate character and strength. Practical significance. Studies was established that breeding of animals of Holstein and Yaroslavl breeds in one herd allows to receive an optimum ratio of milk production with optimal percentage fat and protein. For this herd among animals of the improved genotypes of the Yaroslavl breed, the use of genotypes with a percent of blood in the Holstein breed from 75.1 % to 87.6 % is optimal, which should be taken into account when selecting bulls to the breeding program.


2017 ◽  
Vol 53 ◽  
pp. 222-227
Author(s):  
T. M. Komendant

Objective: to study the dependence of productive longevity of cows of black and motley breed on genetically determined factors - linearity and bloodiness in Holstein breed. Methods: zootechnical, biological, statistical. The materials of the studies were pedigree cards of cows  and bulls, bull catalogs, zootechnical reports on pedigree work with the herd, the cattle base of the Grodno region, and also registers of rearing and rearing of the litter. To study the effect on the longevity of cows and their lifelong productivity of the "linear accessory" factor, all animals that were eliminated in 2006-2014 were divided into groups according to the lines: Adema 25437, Annas Adema 30587, Vis Eidial 933122, Montvik Chiftein 95679, Nico 31652 , Pabst Governera 882933, Reflection Sovering 198998, S. Sensation 1267271, Hiltes Adema 37910. Depending on the bloodiness of the Holstein breed, the animals were divided into four groups: I - up to 25% of Holstein genes, II- 25,0 – 49,9% of the genes of the Holstein genus, III - Holstein blood 50% and more, IV - purebred black-motley cows. Indicators of milk productivity (life-time milk yield (kg) and lifetime yield of milk fat (kg), milk yield for 1 day of lactation (kg), total duration of the lactation period (days) were studied in the experimental animals, and the duration of economic use (lactations). Main results. The results obtained during the statistical processing of data indicate that the cows belonging to the Pabst Governera line 882933 differed in the longest period of economic use among the abandoned animals. Duration of use in animals of this line was 8.43 lactations, which is more than for cows of other lines at 1,72-6,67 lactation. It should be noted that cows belonging to the Adem 25437 lines (6,71 lactations) and S. Sensation 1267271 (5,49 lactations) were also characterized by a rather long period of economic use. Due to the long period of use of the cow, the Pabst Governera line 882933 had the highest indicator of lifelong milk yield – 51535,58 kg of milk and the yield of milk fat – 1914,03. But as for milk for one day of lactation, here the leadership belonged to individuals belonging to the line Vis Eidial 933122 and amounted to 21,19 kg of milk per day. Also, a fairly high indicator of milk yield for one day of lactation was observed in animals belonging to the line Montvik Chiftain 95679 – 21,02 kg of milk. As a result of the research it was found that the largest term of economic use was characterized by low-breed hybrids with Holstein breed (up to 25%) – 3,63 lactations. This group of animals exceeded the individuals of the remaining groups by 0,21-0,76 lactations. It is worth noting that purebred black-motley individuals also differed by a long period of productive use – 3,42 lactations. Conclusions. Analysis of the impact on productive longevity and indicators of lifetime productivity of the "linearity" factor among abandoned animals in the "Progress-Vertelishki" made it possible to establish the existence of significant differences in the duration of economic use of cows of different linear affiliation. Cows of the Pabst Governera line 882933 – 8,43 lactations, 51535,58 kg of milk and 1914,03 kg of milk fat were distinguished by the greatest period of productive longevity, and also by the highest milk yield and milk fat content. It was established that the crossing of black and white cattle with Holstein breed positively influenced the productive longevity of animals, but on condition that the blood on the improving rock did not exceed 25%. Low-breed hybrids with Holstein breed exceeded the animals of other groups by the duration of their economic use by 0,21-0,76 lactations. Thus, we recommend breeding cows of black and motley breeds in the "Progress-Vertelishki", taking into account the impact on productive longevity and milk productivity of such factors as linearity and bloodiness in Holstein breed.


2011 ◽  
Vol 56 (No. 4) ◽  
pp. 151-156 ◽  
Author(s):  
J. Strychalski ◽  
U. Czarnik ◽  
M. Pierzchała ◽  
C.S. Pareek

Current studies on the prion protein (PRNP) gene polymorphism focus primarily on the causative mutations that influence BSE susceptibility in cattle. The specific genetic structure determined by the insertion/deletion (indel) polymorphism within the 23 bp promoter sequence and the 12 bp intron 1 sequence of the PRNP gene, and its genomic location suggest that this polymorphism can be a potential QTL marker. The objective of the present study was to determine whether the indel polymorphism within the promoter sequence (23 bp) and the intron 1 sequence (12 bp) of the PRNP gene can be used as a factor differentiating the values of milk performance traits. The experimental materials comprised 285 primiparous Polish Holstein-Friesian cows, daughters of two dihybrid (23ins-12ins/23del-12del) sires (progeny of sire 1 &ndash; 149 cows, progeny of sire 2 &ndash; 136 cows). The following milk performance traits were analysed: milk yield, milk fat yield, milk protein yield, fat and protein contents of milk during the first 305-day lactation. The polymorphism in the promoter region (23 bp) was found to have a significant (P = 0.040) effect on protein yield and a highly significant (P = 0.007) effect on the protein content of milk. The highest values of these traits were noted in 23 ins/del heterozygotes and the lowest in 23 del/del homozygotes. There was an interrelation between diplotype variants and the concentrations of milk components. The protein content of milk was highly significantly (P = 0.007) higher in 23ins-12ins/23del-12del heterozygotes and significantly (P = 0.028) higher in 23ins-12ins/23ins-12ins homozygotes, compared to 23del-12ins/23del-12del cows. 23ins-12ins/23del-12del heterozygotes were also characterized by a significantly (P = 0.046) higher fat content of milk, in comparison with 23del-12del/23del-12del homozygotes.


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