The Effect of a Biopreparation on Legume (Pisum Sativum)

Biological Preparation ◽

A field experiment with a biological preparation based on the association of nitrogen-fixing bacteria was carried out. As a result, the biological preparation had a positive effect on germination, length and vegetative mass of peas.

Features of growing chickpea in the foothill zone of the Kabardino-Balkarian Republic

E3S Web of Conferences ◽

10.1051/e3sconf/202022202008 ◽

2020 ◽

Vol 222 ◽

pp. 02008

Author(s):

Zalina Tarasheva ◽

Irina Khanieva ◽

Aliy Boziev ◽

Takhir Chapaev ◽

Andemirkan Odizhev

Keyword(s):

Growth Regulators ◽

Growing Season ◽

Nitrogen Fixing ◽

Potassium Sodium ◽

Sodium Humate ◽

Field Germination ◽

Golden Jubilee ◽

In the foothill zone of the Kabardino-Balkarian Republic in 2013-2015, studies were carried out in order to study the effect of growth regulators and rhizotorfin on the formation of elements of the structure of the chickpea crop. The objects of research were the varieties of chickpeas “Golden Jubilee” and “Privo 1”. The background for testing biological preparations was pre-sowing inoculation of seeds with rhizotorphin based on nitrogen-fixing bacteria and their treatment with microelements (P120K60MoV). The following drugs were used: Albit, Alfastim, Potassium / sodium humate with microelements. The use of growth regulators and rhizotorfin has a positive effect on field germination, the duration of the growing season and the yield of chickpea. The analysis of the data in our studies showed that the cultivation of the chickpea variety “Golden Jubilee” in the foothill zone of the Kabardino-Balkarian Republic is more profitable.

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

Influence of Different Amendments on the Dynamics of Nitrogen Fixing Bacteria Free in Chernozem Soil of Dobrogea

10.15835/buasvmcn-agr:6481 ◽

2011 ◽

Vol 68 (1) ◽

Author(s):

Elena DELCĂ ◽

Tatiana PASCU

Keyword(s):

Preliminary Data ◽

Organic Amendments ◽

Chernozem Soil ◽

Nitrogen Fixing ◽

Abundance Dynamics ◽

Microbial Density ◽

Density Values ◽

Dry Soil ◽

The paper presents preliminary data on the abundance dynamics of nitrogen fixing bacteria-free cultivation, the effect of administration of anorganic organic amendments were. Based on 7 experimental variants of cultivation was found that the total number of nitrogen fixing bacteria-free cultivation (cyanobacteria and Azotobacter species) ranged between: 39.58x105CFU/g soil in October (V6) and 326.78x105CFU/g soil in May (V3). When soil was supplemented with manure microbial density showed a significant increase 326.78x105CFU/g dry soil compared with control variant 74.17x105CFU/g soil. Inorganic fertilizers did not have a positive effect on microbial density values, being more or less similar to those reported for the control. Bacterial density increased significantly as value, too, following the administration of specific biofertilizers (Biovin, Bactofil Professional; Mycos Green), up to (V6) 319.81x105CFU/g dry soil in May.

MESORHIZOBIUM CICERІ ND-64 STRAIN — EFFECTIVE MICROSYMBIONT OF MODERN CHICKPEA VARIETIES

Agriciltural microbiology ◽

10.35868/1997-3004.32.18-34 ◽

2021 ◽

Vol 32 ◽

pp. 18-34

Author(s):

V. P. Sydorenko ◽

V. V. Volkohon ◽

S. B. Dimova ◽

K. I. Volkohon ◽

N. L. Lutsenko ◽

...

Keyword(s):

Field Experiment ◽

Reference Strain ◽

Field Experiments ◽

Nitrogenase Activity ◽

Local Population ◽

Steppe Zone ◽

Positive Control ◽

Average Height ◽

Nitrogen Fixing ◽

Nodule Bacteria

Objective. Study the competitiveness, complementarity and symbiotic activity of a new strain of chickpea nodule bacteria Mesorhizobium ciceri ND-64, study the influence of seed inoculation on the productivity and yield of chickpea varieties of Ukrainian selection under their cultivation in different soil and climatic zones of Ukraine. Methods. Serological, gas chromatography, field experiment and statistical. Results. The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12). The use of M. ciceri ND- 64 for pre-sowing bacterization of chickpea seeds under cultivation in the Steppe zone of Ukraine also had a positive effect on increasing the yield of chickpea — by 4 % to 18 % compared to the positive control. Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia. Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown. Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601 and M. ciceri ND-64, isolated from chickpea nodules of Skarb and Pamiat varieties. The competitiveness of M. ciceri ND-64 was determined using the immunological method in the field experiment. It was found that 100 % of nodules have been formed by the representatives of M. ciceri ND-64 in the variant where this serological group was applied for inoculation in the variant with inoculation of the strain M. ciceri ND-64 100 % of the nodules were formed by representatives of this serogroup. Conclusion. M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants, due to its virulence, competitiveness, nitrogen-fixing properties. According to the results of field experiments, it was shown that the new strain, complementary to all studied varieties differing in seed size and shape, type of bush and average height, forms an effective symbiosis for growing chickpea plants both against the active local population of rhizobia and upon the absence of M. cicerі population in the soil. Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12 and strains isolated from the nodules of each study variety. Therefore, M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties in order to form an effective bean-rhizobial symbiosis, increase the productivity of this culture and improve seed quality.

Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020 ◽

Production and analysis of tomato Solanum lycopersicum composite plants carrying the genes of pea Pisum sativum receptors to rhizobial signaling molecules

10.28983/plamic2020.208 ◽

2020 ◽

Author(s):

E. S. Rudaya ◽

E. A. Dolgikh

Keyword(s):

Pisum Sativum ◽

Solanum Lycopersicum ◽

Signaling Molecules ◽

Nitrogen Fixing ◽

Nod Factors ◽

Tomato Plants ◽

Composite Plants ◽

Genes Encoding

The interaction of composite tomato plants carrying constructs with pea genes encoding receptors to Nod factors, with nitrogen-fixing bacteria rhizobia was investigated.

Interactions among Plant Species and Microorganisms in Salt Marsh Sediments

Applied and Environmental Microbiology ◽

10.1128/aem.68.3.1157-1164.2002 ◽

2002 ◽

Vol 68 (3) ◽

pp. 1157-1164 ◽

Cited By ~ 80

Author(s):

David J. Burke ◽

Erik P. Hamerlynck ◽

Dittmar Hahn

Keyword(s):

Microbial Community ◽

Salt Marsh ◽

Mycorrhizal Fungi ◽

Growing Season ◽

Microbial Populations ◽

Nitrogen Fixing ◽

Positive Interaction ◽

Bacterial Populations ◽

Sediment Samples

ABSTRACT The interactions among Spartina patens and sediment microbial populations and the interactions among Phragmites australis and sediment microbial populations were studied at monotypic sites in Piermont Marsh, a salt marsh of the Hudson River north of New York, N.Y., at key times during the growing season. Arbuscular mycorrhizal fungi (AMF) effectively colonized S. patens but not P. australis, and there were seasonal increases and decreases that coincided with plant growth and senescence (17 and 6% of the S. patens root length were colonized, respectively). In sediment samples from the Spartina site, the microbial community and specific bacterial populations were at least twice as large in terms of number and biomass as the microbial community and specific bacterial populations in sediment samples from the Phragmites site, and peak values occurred during reproduction. Members of the domain Bacteria, especially members of the α-, γ-, and δ-subdivisions of the Proteobacteria, were the most abundant organisms at both sites throughout the growing season. The populations were generally more dynamic in samples from the Spartina site than in samples from the Phragmites site. No differences between the two sites and no differences during the growing season were observed when restriction fragment length polymorphism analyses of nifH amplicons were performed in an attempt to detect shifts in the diversity of nitrogen-fixing bacteria. Differences were observed only in the patterns generated by PCR or reverse transcription-PCR for samples from the Spartina site, suggesting that there were differences in the overall and active populations of nitrogen-fixing bacteria. Regression analyses indicated that there was a positive interaction between members of the δ-subdivision of the Proteobacteria and root biomass but not between members of the δ-subdivision of the Proteobacteria and macroorganic matter at both sites. In samples from the Spartina site, there were indications that there were bacterium-fungus interactions since populations of members of the α-subdivision of the Proteobacteria were negatively associated with AMF colonization and populations of members of the γ-subdivision of the Proteobacteria were positively associated with AMF colonization.

MESORHIZOBIUM CICERІ ND-64 STRAIN — EFFECTIVE MICROSYMBIONT OF MODERN CHICKPEA VARIETIES

Agriciltural microbiology ◽

10.35868/1997-3004.32.3-17 ◽

2021 ◽

Vol 32 ◽

pp. 3-17

Author(s):

О. V. Lohosha ◽

Yu. O. Vorobei ◽

I. V. Volkova ◽

T. O. Usmanova

Keyword(s):

Field Experiment ◽

Reference Strain ◽

Field Experiments ◽

Nitrogenase Activity ◽

Local Population ◽

Steppe Zone ◽

Positive Control ◽

Average Height ◽

Nitrogen Fixing ◽

Nodule Bacteria

Objective. Study the competitiveness, complementarity and symbiotic activity of a new strain of chickpea nodule bacteria Mesorhizobium ciceri ND-64, study the influence of seed inoculation on the productivity and yield of chickpea varieties of Ukrainian selection under their cultivation in different soil and climatic zones of Ukraine. Methods. Serological, gas chromatography, field experiment and statistical. Results. The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12). The use of M. ciceri ND- 64 for pre-sowing bacterization of chickpea seeds under cultivation in the Steppe zone of Ukraine also had a positive effect on increasing the yield of chickpea — by 4 % to 18 % compared to the positive control. Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia. Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown. Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601 and M. ciceri ND-64, isolated from chickpea nodules of Skarb and Pamiat varieties. The competitiveness of M. ciceri ND-64 was determined using the immunological method in the field experiment. It was found that 100 % of nodules have been formed by the representatives of M. ciceri ND-64 in the variant where this serological group was applied for inoculation in the variant with inoculation of the strain M. ciceri ND-64 100 % of the nodules were formed by representatives of this serogroup. Conclusion. M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants, due to its virulence, competitiveness, nitrogen-fixing properties. According to the results of field experiments, it was shown that the new strain, complementary to all studied varieties differing in seed size and shape, type of bush and average height, forms an effective symbiosis for growing chickpea plants both against the active local population of rhizobia and upon the absence of M. cicerі population in the soil. Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12 and strains isolated from the nodules of each study variety. Therefore, M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties in order to form an effective bean-rhizobial symbiosis, increase the productivity of this culture and improve seed quality.

Influence of metal nanocarboxylates and different water supply conditions on efficiency of soybean-rhizobial symbiotic systems

Regulatory Mechanisms in Biosystems ◽

10.15421/022152 ◽

2021 ◽

Vol 12 (3) ◽

pp. 383-390

Author(s):

S. Y. Kots ◽

L. I. Rybachenko ◽

T. P. Mamenko ◽

K. P. Kukol ◽

P. P. Pukhtaievych ◽

...

Keyword(s):

Nitrogen Fixation ◽

Water Stress ◽

Water Supply ◽

Negative Impact ◽

Molecular Nitrogen ◽

Soybean Seed ◽

Bacterial Suspension ◽

Nitrogen Fixing ◽

Nodule Bacteria ◽

Insufficient water supply is one of the main factors that significantly reduce the activity of nitrogen fixation by legume-rhizobial symbiotic systems. That is why comprehensive research on aspects of their resistance to water stress and the search for scientifically substantiated ways to improve the existing ones and develop modern, competitive technologies of growing legumes in arid conditions are becoming especially relevant. The aim of the work was to investigate the processes of formation and functioning of soybean-rhizobial symbiotic systems developed under conditions of different water supply and influence of nanocarboxylates of cobalt, ferum, germanium, chromium, сuprum and molybdenum. The nanoparticles of specified metal nanocarboxylates were used as components of the inoculation suspension of rhizobia of Tn5 mutant B1-20 for soybean seed treatment. A model drought lasting 14 days was created by controlled irrigation. Microbiological and physiological research methods were used in the study. We determined that insufficient water supply caused a significant decrease in the nodulation potential of rhizobia and the intensity of molecular nitrogen fixation by symbiotic systems formed with the participation of soybean plants and nodule bacteria without adding these metal nanocarboxylates to the inoculation suspension. Application of most of the metal nanocarboxylates used as components of the inoculation suspension mitigated the negative impact of stress on the investigated parameters. The study revealed the stimulating effect of cobaltnanocarboxylate on the activity of molecular nitrogen fixation, which was more pronounced in the conditions of insufficient water supply. Symbiotic soybean systems formed with the participation of nodule bacteria containing germaniumcarboxylate nanoparticles were proved to be the least sensitive to the negative impact of insufficient water supply. This was indicated by high rates of nodulation and nitrogen-fixing activity compared with other studied symbiotic systems. We confirmed that the addition of chromium nanocarboxylate to the inoculation suspension of rhizobia provided the highest rates of nodulation and nitrogen-fixing activity of soybean root nodules under optimal growing conditions and, at the same time, had no noticeable positive effect under water stress. We determined that сuprum and molybdenum nanocarboxylates, as components of the inoculation suspension, regardless of the water supply level, had a less notable positive effect on the processes of nodule formation and nitrogen fixation, and in some cases even led to a decrease in the investigated values for control plants. Thus, the study demonstrated that the use of germanium, cobalt and ferum nanocarboxylates as components of the bacterial suspension helped to increase the adaptation of the formed legume-rhizobial symbiotic systems to water stress, as evidenced by the maximum indexes of nodulation and molecular nitrogen fixation in the context of insufficient water supply and recovery of their level to optimal after the stress influence had ended. Based on the results, it was concluded that inoculation of seeds by the complex bacterial preparations made on the basis of Bradyrhizobium japonicum B1-20 with a content of germanium, cobalt and ferum nanocarboxylates in the concentration of 1:1000 can become one of the important means in soybean growing technologies of increasing the nitrogen-fixing potential and resistance of plants to insufficient water supply.