A Hybrid Bioprinting Approach for Scale-Up Tissue Fabrication

2014 ◽  
Vol 136 (6) ◽  
Author(s):  
Yin Yu ◽  
Yahui Zhang ◽  
Ibrahim T. Ozbolat
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Large Scale ◽  
Vascular System ◽  
Scale Up ◽  
Organ Printing ◽  
Promising Application ◽  
Organ Models

Tissue engineering has been focused on the fabrication of vascularized 3D tissue for decades. Most recently, bioprinting, especially tissue and organ printing, has shown great potential to enable automated robotic-based fabrication of 3D vascularized tissues and organs that are readily available for in vitro studies or in vivo transplantation. Studies have demonstrated the feasibility of the tissue printing process through bioprinting of scaffold-free cellular constructs that are able to undergo self-assembly for tissue formation; however, they are still limited in size and thickness due to the lack of a vascular network. In this paper, we present a framework concept for bioprinting 3D large-scale tissues with a perfusable vascular system in vitro to preserve cell viability and tissue maturation. With the help of a customized Multi-Arm Bioprinter (MABP), we lay out a hybrid bioprinting system to fabricate scale-up tissues and organ models and demonstrated envision its promising application for in vitro tissue engineering and its potential for therapeutic purposes with our proof of concept study.

Collagen/silica nanocomposites and hybrids for bone tissue engineering

2013 ◽  
Vol 2 (4) ◽  
pp. 427-447 ◽  
Author(s):  
Bapi Sarker ◽  
Stefan Lyer ◽  
Andreas Arkudas ◽  
Aldo R. Boccaccini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Self Assembly ◽  
Structural Integrity ◽  
Organic Matrix ◽  
Angiogenic Potential ◽  
Silica Nanocomposites

AbstractCollagen is increasingly attracting attention for bone tissue engineering applications. However, due to its low mechanical properties, applications including mechanical loads or requiring structural integrity are limited. To tackle this handicap, collagen can be combined with (nanoscale) silica in a variety of composite materials that are attractive for bone tissue engineering. Considering research carried out in the past 15 years, this article reviews the literature discussing the development of silica/collagen composites that have been synthesized by adding silica from different sources as inorganic bioactive material to collagen as organic matrix. Different routes for the fabrication of collagen/silica composites are presented, focusing on nanocomposites. In vitro cell bioactivity studies demonstrated the osteogenic and, in some cases, angiogenic potential of the composites. Relevant in vivo studies discussing integration of the materials in bone tissue are discussed. Due to the understanding of possible interaction between silicon species and collagen, the effect of different silica precursors on the collagen self-assembly process is also discussed. On the basis of literature results and as discussed in this review, collagen/silica nanocomposites and hybrids represent attractive biomaterials for bone regeneration applications.

scholarly journals 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering

2021 ◽  
Vol 8 (11) ◽  
pp. 185
Author(s):  
Amit Panwar ◽  
Prativa Das ◽  
Lay Poh Tan
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Liver Tissue ◽  
3D Culture ◽  
Culture Method ◽  
Culture Conditions ◽  
Phenotypic Properties ◽  
Liver Tissue Engineering

Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering.

scholarly journals Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors

2020 ◽  
Vol 2020 ◽  
pp. 1-23 ◽  
Author(s):  
Vincent Roy ◽  
Brice Magne ◽  
Maude Vaillancourt-Audet ◽  
Mathieu Blais ◽  
Stéphane Chabaud ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tumor Microenvironment ◽  
Self Assembly ◽  
The Self ◽  
Human Organ ◽  
Assembly Method ◽  
Cancer Models ◽  
Organ Specific

Cancer research has considerably progressed with the improvement of in vitro study models, helping to understand the key role of the tumor microenvironment in cancer development and progression. Over the last few years, complex 3D human cell culture systems have gained much popularity over in vivo models, as they accurately mimic the tumor microenvironment and allow high-throughput drug screening. Of particular interest, in vitrohuman 3D tissue constructs, produced by the self-assembly method of tissue engineering, have been successfully used to model the tumor microenvironment and now represent a very promising approach to further develop diverse cancer models. In this review, we describe the importance of the tumor microenvironment and present the existing in vitro cancer models generated through the self-assembly method of tissue engineering. Lastly, we highlight the relevance of this approach to mimic various and complex tumors, including basal cell carcinoma, cutaneous neurofibroma, skin melanoma, bladder cancer, and uveal melanoma.

Tissue Engineering of Fibroblast Constructs and Anisotropic Collagen Gels

2002 ◽  
Vol 724 ◽  
Author(s):  
Sarah Calve ◽  
Ellen Arruda ◽  
Robert Dennis ◽  
Karl Grosh ◽  
Krystyna Pasyk
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Cell Layer ◽  
Fetal Bovine Serum ◽  
Self Organization ◽  
Collagen Gels ◽  
Confluent Cell ◽  
Fetal Bovine

AbstractThe creation of an in vitro functional tendon construct will enable testing of the influence of mechanics and nutrients on the development and remodeling of tendon under known controlled stimuli which is difficult to achieve in vivo. Tendon constructs were engineered in vitrovia stress-mediated self organization of fibroblasts and ECM on a laminin coated elastomer substrate. Varying the laminin density and the amount of fetal bovine serum on the substrate affected the ability of tendon fibroblasts to form a confluent cell layer and the time to layer delamination. Understanding the factors that promote self-assembly of tendon constructs will enable their combination with already developed in vitro muscle constructs.

Mechanobiology in Tendon, Ligament, and Skeletal Muscle Tissue Engineering

2021 ◽  
Vol 143 (7) ◽  
Author(s):  
Michael T. K. Bramson ◽  
Sarah K. Van Houten ◽  
David T. Corr
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Mechanical Loading ◽  
Mechanical Stimulation ◽  
Self Assembly ◽  
Tissue Constructs ◽  
Active Contraction ◽  
Matrix Production

Abstract Tendon, ligament, and skeletal muscle are highly organized tissues that largely rely on a hierarchical collagenous matrix to withstand high tensile loads experienced in activities of daily life. This critical biomechanical role predisposes these tissues to injury, and current treatments fail to recapitulate the biomechanical function of native tissue. This has prompted researchers to pursue engineering functional tissue replacements, or dysfunction/disease/development models, by emulating in vivo stimuli within in vitro tissue engineering platforms—specifically mechanical stimulation, as well as active contraction in skeletal muscle. Mechanical loading is critical for matrix production and organization in the development, maturation, and maintenance of native tendon, ligament, and skeletal muscle, as well as their interfaces. Tissue engineers seek to harness these mechanobiological benefits using bioreactors to apply both static and dynamic mechanical stimulation to tissue constructs, and induce active contraction in engineered skeletal muscle. The vast majority of engineering approaches in these tissues are scaffold-based, providing interim structure and support to engineered constructs, and sufficient integrity to withstand mechanical loading. Alternatively, some recent studies have employed developmentally inspired scaffold-free techniques, relying on cellular self-assembly and matrix production to form tissue constructs. Whether utilizing a scaffold or not, incorporation of mechanobiological stimuli has been shown to improve the composition, structure, and biomechanical function of engineered tendon, ligament, and skeletal muscle. Together, these findings highlight the importance of mechanobiology and suggest how it can be leveraged to engineer these tissues and their interfaces, and to create functional multitissue constructs.

scholarly journals Mesenchymal Stem Cell Derived Extracellular Vesicles for Tissue Engineering and Regenerative Medicine Applications

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 991 ◽  
Author(s):  
Dimitrios Tsiapalis ◽  
Lorraine O’Driscoll
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Extracellular Vesicles ◽  
Scale Up ◽  
In Vivo Studies ◽  
Substantial Evidence ◽  
Beneficial Effects ◽  
Therapeutic Properties

Mesenchymal stem cells (MSCs) are being extensively investigated for their potential in tissue engineering and regenerative medicine. However, recent evidence suggests that the beneficial effects of MSCs may be manifest by their released extracellular vesicles (EVs); typically not requiring the administration of MSCs. This evidence, predominantly from pre-clinical in vitro and in vivo studies, suggests that MSC-EVs may exhibit substantial therapeutic properties in many pathophysiological conditions, potentially restoring an extensive range of damaged or diseased tissues and organs. These benefits of MSC EVs are apparently found, regardless of the anatomical or body fluid origin of the MSCs (and include e.g., bone marrow, adipose tissue, umbilical cord, urine, etc). Furthermore, early indications suggest that the favourable effects of MSC-EVs could be further enhanced by modifying the way in which the donor MSCs are cultured (for example, in hypoxic compared to normoxic conditions, in 3D compared to 2D culture formats) and/or if the EVs are subsequently bio-engineered (for example, loaded with specific cargo). So far, few human clinical trials of MSC-EVs have been conducted and questions remain unanswered on whether the heterogeneous population of EVs is beneficial or some specific sub-populations, how best we can culture and scale-up MSC-EV production and isolation for clinical utility, and in what format they should be administered. However, as reviewed here, there is now substantial evidence supporting the use of MSC-EVs in tissue engineering and regenerative medicine and further research to establish how best to exploit this approach for societal and economic benefit is warranted.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

In vitro- / in vivo- Untersuchungen zur Biokompatibilität und Bioresorption amorpher Kieselgelfaser für das Tissue engineering humaner Knorpelgewebe

2004 ◽  
Vol 83 (02) ◽  
Author(s):  
A Haisch ◽  
A Evers ◽  
K Jöhrens-Leder ◽  
S Jovanovic ◽  
B Sedlmaier ◽  
...  
Keyword(s):  
Tissue Engineering
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