Collapse Temperature of Solutions Important for Lyopreservation of Living Cells at Ambient Temperature

2010 ◽  
Author(s):  
Geer Yang ◽  
Kyle Gilstrap ◽  
Aili Zhang ◽  
Lisa X. Xu ◽  
Xiaoming He
Keyword(s):  
Ambient Temperature ◽  
Modern Medicine ◽  
Living Cells ◽  
Therapeutic Tool ◽  
Collapse Temperature ◽  
Cell Sources ◽  
Increasing Demand ◽  
Long Term Preservation ◽  
Cell Preservation

With recent advances in cell-based medical technologies such as tissue engineering and regenerative medicine, living cells are becoming more and more important as a therapeutic tool in modern medicine. Due to the limited availability of cell sources, effective long-term preservation of living cells is of great importance to the eventual success of these emerging technologies. Cell preservation can be achieved by cooling/freezing the cells to a cryogenic temperature (cryopreservation) and/or drying the cells to preserve at ambient temperature (lyopreservation). Due to the high cost of maintaining and inconvenience of transporting cryopreserved cells, there is an increasing demand for cell lyopreservation.

scholarly journals Stabilisation of Some Vegetable Oils by Sugarcane Leaf Extract at Ambient Temperature

2016 ◽  
Vol 59 (3) ◽  
pp. 155-163
Author(s):  
Muhammad Nadeem ◽  
Muhammad Waqar Azeem
Keyword(s):  
Ambient Temperature ◽  
Vegetable Oils ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Pet Bottles ◽  
Canola Oils ◽  
Long Term Preservation

The present study was aimed to assess the antioxidant activity of ethanolic sugarcane leaf extractfor the stabilisation of sunflower, (SFO), soybean and (SBO) canola oils (CO) at ambient temperature.SFO, SBO and CO were added with 600 ppm sugarcane leaf extract, filled in transparent PET bottles,stored at ambient temperature for 180 days, sampled at 0, 60, 120 and 180 days for the assessment ofoxidative stability. Total phenolic content in sugarcane leaf extract (SLE) was 724.3 (mg GAE/100g).2,2,diphenyl- 2 picrylhydrazyl free radical scavenging activity of SLE was 76% as compared to 88% inbutylated hydroxyl toluene. C18:1 and C18:2 in fresh, 6 months stored controls and SLE supplementedSFO were 46.12%, 42.59%, 44.91% and 47.15%, 40.29, 43.13%, respectively. C18:2 and C18:3 in freshand 180 days stored control and SLE supplemented SBO were 51.19%, 45.61%, 48.97% and 6.19%, 3.37%and 5.67%, respectively. Similar trend was also recorded in canola oil. Induction period of supplementedvegetable oil was higher than the un-supplemented samples (P<0.05). Viscosity and specific gravity ofsupplemented vegetable oils were not different from non-supplemented samples. Sensory characteristicsof SLE supplemented vegetable oils were not different from the control. Sugarcane leaf extract can beused for the long term preservation of SFO, SBO and CO at ambient temperature.

The Effect of Synthetic Ice Blockers on Thermal Expansion of the Cryoprotective Cocktail DP6

2011 ◽  
Author(s):  
David P. Eisenberg ◽  
Yoed Rabin
Keyword(s):  
Potential Method ◽  
Living Cells ◽  
Ice Crystals ◽  
Cryoprotective Agents ◽  
Medical Need ◽  
The Subject ◽  
High Cooling Rates ◽  
Cryopreservation Techniques ◽  
Long Term Preservation

Cryopreservation is the only alternative for long-term preservation of high-quality biomaterials, where the availability of reliable techniques for preservation of multicellular structures and organs represents an unmet medical need. Developing cryopreservation techniques revolves around controlling the formation of ice crystals, which is known to be lethal to living cells. Cryopreservation is typically achieved in the presence of cryoprotective agents (CPAs), which exhibit a dramatic increase in viscosity with decreasing temperature. Subject to high cooling rates, the rapidly elevating viscosity of the CPA suppresses ice crystallization and promotes vitrification (vitreous means glassy in Latin). Unfortunately, available CPAs are known to be toxic at the relevant concentrations which permit vitrification. One potential method of reducing CPA concentration, and thereby achieving conditions more favorable to the tissue, is with the introduction of the so-called synthetic ice blockers (SIBs)—the subject matter of the current study.

Collapse temperature of solutions important for lyopreservation of living cells at ambient temperature

2010 ◽  
pp. n/a-n/a
Author(s):  
Geer Yang ◽  
Kyle Gilstrap ◽  
Aili Zhang ◽  
Lisa X. Xu ◽  
Xiaoming He
Keyword(s):  
Ambient Temperature ◽  
Living Cells ◽  
Collapse Temperature

Problems of treatment of primary openangle glaucoma in combination with thrombosis of the central retinal vein

GlaucomaNews ◽  
2020 ◽  
pp. 65-69
Author(s):  
T.E. Lipatkina ◽  
◽  
Е.V. Karlova ◽  
A.V. Zolotarev ◽  
◽  
...  
Keyword(s):  
Macular Edema ◽  
Primary Open Angle Glaucoma ◽  
Antihypertensive Drugs ◽  
Open Angle Glaucoma ◽  
Vascular Wall ◽  
Open Angle ◽  
Retinal Edema ◽  
Long Term Preservation ◽  
Central Retinal Vein

Patients with primary open-angle glaucoma (POAG) and ophthalmic hypertension have an increased likelihood of developing occlusions (thrombosis) of the central retinal vein. Different groups of antihypertensive drugs differ in their mechanism of action and may affect concomitant ocular pathology, in particular, retinal edema, which occurs, for example, in occlusion of the central retinal vein. Used in most patients with glaucoma, prostaglandin analogs can contribute to the long-term preservation of macular edema due to the effect on the permeability of the vascular wall. Preparations of other pharmacological groups, reducing the production of aqueous humor, on the contrary, may contribute to its regression. Therefore, the question of choosing a drug for antihypertensive therapy in patients with primary open-angle glaucoma and concomitant macular edema is relevant and is for further study.

Possibilities and prospects of conservative therapy for endometriosis, as a chronical progressing disease (review of literature)

GYNECOLOGY ◽  
2020 ◽  
Vol 22 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Tatyana Yu. Pestrikova ◽  
Elena A. Yurasova ◽  
Igor V. Yurasov
Keyword(s):  
Quality Of Life ◽  
Abnormal Uterine Bleeding ◽  
Modern Medicine ◽  
Reproductive Age ◽  
Aim Analysis ◽  
Scientific Publications ◽  
Terra Incognita ◽  
Positive Effect

Relevance. Endometriosis is a common gynecological disease that affects up to 10% of women of reproductive age worldwide and is the main cause of pain and infertility. Endometriosis is a disease, although it has been known for a long time, nevertheless, in many ways it represents terra incognita for modern medicine. Aim. Analysis of literature on the feasibility of long-term and the use of the drug dienogest 2 mg (Vizanne), which has a positive effect on the quality of life of patients with endometriosis. Materials and methods. To write this review, a search was made for domestic and foreign publications in Russian and international search engines (PubMed, eLibrary, etc.) over the past 13 years. The review included articles from peer-reviewed literature. Results. The review presents data on the difficulties of verifying the diagnosis of endometriosis due to a combination of this pathology with pain, infertility, abnormal uterine bleeding. The pathogenesis of the origin of endometriosis-associated pain is presented. The efficacy of the use of the drug dienogest (Vizanne), which has a powerful antiproliferative effect that reduces the main symptoms of endometriosis (pain, bleeding), is substantiated. The expediency of long-term and safe use of the drug dienogest (Vizanne), which has a positive effect on the quality of life of patients with endometriosis, has been proved. Conclusions. Numerous scientific publications confirm the feasibility of prolonged use of the drug dienogest (Vizanne), to achieve remission during endometriosis.

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