Stretch-Induced Stress Fiber Remodeling and MAPK Activations Depend on Mechanical Strain Rate

2011 ◽  
Author(s):  
Hui-Ju Hsu ◽  
Andrea Locke ◽  
Susan Q. Vanderzyl ◽  
Roland Kaunas
Keyword(s):  
Cell Function ◽  
Myosin Ii ◽  
Mechanical Strain ◽  
Optimal Level ◽  
Stress Fiber ◽  
Stress And Strain ◽  
Structural Elements ◽  
Cell Matrix ◽  
Induced Stress ◽  
The Matrix

Actin stress fibers (SFs), bundles of actin filaments crosslinked by α-actinin and myosin II in non-muscle cells, are mechanosensitive structural elements that respond to applied stress and strain to regulate cell morphology, signal transduction and cell function. Results from various studies indicate that myosin-generated contraction extends SFs beyond their unloaded lengths and cells maintain fiber strain at an optimal level that depends on actomyosin activity (Lu et al., 2008). Stretching the matrix upon which cells adhere perturbs the cell-matrix traction forces and cells respond by actively re-establishing the preexisting level of force (Brown et al., 1998; Gavara et al., 2008). We have developed a sarcomeric model of SF networks (Kaunas et al., 2011) to predict the effects of stretch on SF reorganization depending on the rates of matrix stretching, SF turnover, and SF stress relaxation.

Recent Advances in Biohybrid Materials for Tissue Engineering and Regenerative Medicine

2016 ◽  
Vol 04 (01) ◽  
pp. 1640001 ◽  
Author(s):  
Ying Wan ◽  
Xing Li ◽  
Shenqi Wang
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cell Function ◽  
Rapid Prototype ◽  
Artificial Organs ◽  
Specific Cell ◽  
Cell Matrix ◽  
Matrix Surface ◽  
The Matrix ◽  
Cell Matrix Interactions

Biohybrid materials play an important role in tissue engineering, artificial organs and regenerative medicine due to their regulation of cell function through specific cell–matrix interactions involving integrins, mostly those of fibroblasts and myofibroblasts, and ligands on the matrix surface, which have become current research focus. In this paper, recent progress of biohybrid materials, mainly including main types of biohybrid materials, rapid prototype (RP) technique for construction of 3D biohybrid materials, was reviewed in detail; moreover, their applications in tissue engineering, artificial organs and regenerative medicine were also reviewed in detail. At last, we address the challenges biohybrid materials may face.

scholarly journals Stretch-Induced Stress Fiber Remodeling and the Activations of JNK and ERK Depend on Mechanical Strain Rate, but Not FAK

PLoS ONE ◽  
2010 ◽  
Vol 5 (8) ◽  
pp. e12470 ◽  
Author(s):  
Hui-Ju Hsu ◽  
Chin-Fu Lee ◽  
Andrea Locke ◽  
Susan Q. Vanderzyl ◽  
Roland Kaunas
Keyword(s):  
Strain Rate ◽  
Mechanical Strain ◽  
Stress Fiber ◽  
Induced Stress

Regulation of Stretch-Induced Mechanotransduction by Cytoskeletal Tension

2010 ◽  
Author(s):  
Hui-Ju Hsu ◽  
Andrea K. Locke ◽  
Susan Q. Vanderzyl ◽  
Chin-Fu Lee ◽  
Roland Kaunas
Keyword(s):  
Endothelial Cells ◽  
Muscle Cells ◽  
Stress Fiber ◽  
Stress Fibers ◽  
Traction Forces ◽  
Cell Matrix ◽  
The Matrix ◽  
Cytoskeletal Tension

Cytoskeletal tension enables cells to adhere, spread, contract, and migrate. In adherent, non-muscle cells such as endothelial cells and fibroblasts, tension is generated by actomyosin stress fibers applying traction forces to cell-matrix adhesions. Tension extends stress fibers beyond their unloaded lengths and cells maintain a preferred level of fiber strain that depends on actomyosin activity (Lu 2008). Stretching the matrix upon which cells adhere perturbs the cell-matrix traction forces and cells respond by actively re-establishing the pre-existing level of force (Gavara 2006). Sudden large increases or decreases in strain result in rapid stress fiber disassembly and reassembly (Lu 2008), suggesting that perturbing fiber strain from a preferred level stimulates stress fiber turnover.

Designing TIMP (tissue inhibitor of metalloproteinases) variants that are selective metalloproteinase inhibitors

2003 ◽  
Vol 70 ◽  
pp. 201-212 ◽  
Author(s):  
Hideaki Nagase ◽  
Keith Brew
Keyword(s):  
Three Dimensional ◽  
Therapeutic Interventions ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Structural Basis ◽  
Structural Elements ◽  
Ridge Structure ◽  
Extracellular Matrix Components ◽  
Metalloproteinase Inhibitors ◽  
The Matrix ◽  
A Disintegrin And Metalloproteinase

The tissue inhibitors of metalloproteinases (TIMPs) are endogenous inhibitors of the matrix metalloproteinases (MMPs), enzymes that play central roles in the degradation of extracellular matrix components. The balance between MMPs and TIMPs is important in the maintenance of tissues, and its disruption affects tissue homoeostasis. Four related TIMPs (TIMP-1 to TIMP-4) can each form a complex with MMPs in a 1:1 stoichiometry with high affinity, but their inhibitory activities towards different MMPs are not particularly selective. The three-dimensional structures of TIMP-MMP complexes reveal that TIMPs have an extended ridge structure that slots into the active site of MMPs. Mutation of three separate residues in the ridge, at positions 2, 4 and 68 in the amino acid sequence of the N-terminal inhibitory domain of TIMP-1 (N-TIMP-1), separately and in combination has produced N-TIMP-1 variants with higher binding affinity and specificity for individual MMPs. TIMP-3 is unique in that it inhibits not only MMPs, but also several ADAM (a disintegrin and metalloproteinase) and ADAMTS (ADAM with thrombospondin motifs) metalloproteinases. Inhibition of the latter groups of metalloproteinases, as exemplified with ADAMTS-4 (aggrecanase 1), requires additional structural elements in TIMP-3 that have not yet been identified. Knowledge of the structural basis of the inhibitory action of TIMPs will facilitate the design of selective TIMP variants for investigating the biological roles of specific MMPs and for developing therapeutic interventions for MMP-associated diseases.

scholarly journals Coupled THM and Matrix Stability Modeling of Hydroshearing Stimulation in a Coupled Fracture-Matrix Hot Volcanic System

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Yong Xiao ◽  
Jianchun Guo ◽  
Hehua Wang ◽  
Lize Lu ◽  
John McLennan ◽  
...  
Keyword(s):  
Heat Transfer ◽  
Thermal Conduction ◽  
Dominant Role ◽  
Injection Well ◽  
Time Step ◽  
Volcanic System ◽  
Induced Stress ◽  
The Matrix ◽  
Matrix Stability ◽  
The Stability

A coupled thermal-hydraulic-mechanical (THM) model is developed to simulate the combined effect of fracture fluid flow, heat transfer from the matrix to injected fluid, and shearing dilation behaviors in a coupled fracture-matrix hot volcanic reservoir system. Fluid flows in the fracture are calculated based on the cubic law. Heat transfer within the fracture involved is thermal conduction, thermal advection, and thermal dispersion; within the reservoir matrix, thermal conduction is the only mode of heat transfer. In view of the expansion of the fracture network, deformation and thermal-induced stress model are added to the matrix node’s in situ stress environment in each time step to analyze the stability of the matrix. A series of results from the coupled THM model, induced stress, and matrix stability indicate that thermal-induced aperture plays a dominant role near the injection well to enhance the conductivity of the fracture. Away from the injection well, the conductivity of the fracture is contributed by shear dilation. The induced stress has the maximum value at the injection point; the deformation-induced stress has large value with smaller affected range; on the contrary, thermal-induced stress has small value with larger affected range. Matrix stability simulation results indicate that the stability of the matrix nodes may be destroyed; this mechanism is helpful to create complex fracture networks.

Weight loading young chicks inhibits bone elongation and promotes growth plate ossification and vascularization

2005 ◽  
Vol 98 (6) ◽  
pp. 2381-2389 ◽  
Author(s):  
A. Reich ◽  
N. Jaffe ◽  
A. Tong ◽  
I. Lavelin ◽  
O. Genina ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Growth Plate ◽  
Developmental Process ◽  
Mechanical Strain ◽  
Cell Counting ◽  
Mechanical Stimuli ◽  
Growth Plates ◽  
Average Width ◽  
The Matrix ◽  
Weight Loading

The mechanical stimuli resulting from weight loading play an important role in mature bone remodeling. However, the effect of weight loading on the developmental process in young bones is less well understood. In this work, chicks were loaded with bags weighing 10% of their body weight during their rapid growth phase. The increased load reduced the length and diameter of the long bones. The average width of the bag-loaded group's growth plates was 75 ± 4% that of the controls, and the plates showed increased mineralization. Northern blot analysis, in situ hybridization, and longitudinal cell counting of mechanically loaded growth plates showed narrowed expression zones of collagen types II and X compared with controls, with no differences between the relative proportions of those areas. An increase in osteopontin (OPN) expression with loading was most pronounced at the bone-cartilage interface. This extended expression overlapped with tartarate-resistant acid phosphatase staining and with the front of the mineralized matrix in the chondro-osseous junction. Moreover, weight loading enhanced the penetration of blood vessels into the growth plates and enhanced the gene expression of the matrix metalloproteinases MMP9 and MMP13 in those growth plates. On the basis of these results, we speculate that the mechanical strain on the chondrocytes in the growth plate causes overexpression of OPN, MMP9, and MMP13. The MMPs enable penetration of the blood vessels, which carry osteoclasts and osteoblasts. OPN recruits the osteoclasts to the cartilage-bone border, thus accelerating cartilage resorption in this zone and subsequent ossification which, in turn, contributes to the observed phenotype of narrower growth plate and shorter bones.

scholarly journals Влияние режима получения образцов и термообработки на локальную структуру халькогенидного полупроводника Ge-=SUB=-2-=/SUB=-Sb-=SUB=-2-=/SUB=-Te-=SUB=-5-=/SUB=-

2022 ◽  
Vol 56 (3) ◽  
pp. 291
Author(s):  
С.Н. Гарибова ◽  
А.И. Исаев ◽  
С.И. Мехтиева ◽  
С.У. Атаева ◽  
Р.И. Алекперов
Keyword(s):  
Heat Treatment ◽  
Thermal Processing ◽  
Thermal Evaporation ◽  
Amorphous State ◽  
Hexagonal Structure ◽  
Structural Elements ◽  
X Ray Diffraction ◽  
X Ray ◽  
The Matrix ◽  
Close Range

Specifics of "amorphous state - crystal" phase transitions in dependence on the samples obtaining method and thermal processing, as well as changes in the structure and close range order in the arrangement of the atoms of Ge20Sb20.5Te51 chalcogenide semiconductors have been studied by the x-ray diffraction and Raman spectroscopy. It has been shown that Ge20Sb20.5Te51 films obtained by thermal evaporation on an unheated substrate are amorphous; after heat treatment at 220 and 400 °C, transform into a crystalline phase with a cubic and hexagonal structure. The chemical bonds and the main structural elements that form the matrix of the investigated objects, as well as the changes that occur in them during heat treatment, have been determined.

scholarly journals Tuning cell migration: contractility as an integrator of intracellular signals from multiple cues

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 1819 ◽  
Author(s):  
Francois Bordeleau ◽  
Cynthia A. Reinhart-King
Keyword(s):  
Cell Migration ◽  
Growth Factors ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Multiple Cues ◽  
Cell Contractility ◽  
Cell Matrix ◽  
Intracellular Signals ◽  
Physical Cues ◽  
The Matrix

There has been immense progress in our understanding of the factors driving cell migration in both two-dimensional and three-dimensional microenvironments over the years. However, it is becoming increasingly evident that even though most cells share many of the same signaling molecules, they rarely respond in the same way to migration cues. To add to the complexity, cells are generally exposed to multiple cues simultaneously, in the form of growth factors and/or physical cues from the matrix. Understanding the mechanisms that modulate the intracellular signals triggered by multiple cues remains a challenge. Here, we will focus on the molecular mechanism involved in modulating cell migration, with a specific focus on how cell contractility can mediate the crosstalk between signaling initiated at cell-matrix adhesions and growth factor receptors.

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