Therapeutic Effects of Antibiotics against Enterohemorrhagic Escherichia coli (EHEC) O157: H7 (O157)

Interests in using biological agents for control of human pathogens on vegetable seeds are rising. This study evaluated whether probiotic bacterium Lactobacillus rhamnosus GG, bacterial strains previously used as biocontrol agents in plant science, as well as a selected plant pathogen could compete with foodborne human pathogens, such as Salmonella enterica and enterohemorrhagic Escherichia coli (EHEC), for growth in microbiological media and attachment to vegetable seeds; and to determine whether the metabolites in cell-free supernatants of competitive bacterial spent cultures could inhibit the growth of the two pathogens. The results suggest that the co-presence of competitive bacteria, especially L. rhamnosus GG, significantly (p < 0.05) inhibited the growth of Salmonella and EHEC. Cell-free supernatants of L. rhamnosus GG cultures significantly reduced the pathogen populations in microbiological media. Although not as effective as L. rhamnosus GG in inhibiting the growth of Salmonella and EHEC, the biocontrol agents were more effective in competing for attachment to vegetable seeds. The study observed the inhibition of human bacterial pathogens by competitive bacteria or their metabolites and the competitive attachment to sprout seeds among all bacteria involved. The results will help strategize interventions to produce vegetable seeds and seed sprouts free of foodborne pathogens.

Download Full-text

Quantification of enterohemorrhagic Escherichia coli O157:H7 protein abundance by high-throughput proteome

PLoS ONE ◽

10.1371/journal.pone.0208520 ◽

2018 ◽

Vol 13 (12) ◽

pp. e0208520 ◽

Cited By ~ 1

Author(s):

Wanderson Marques Da Silva ◽

Jinlong Bei ◽

Natalia Amigo ◽

María Pía Valacco ◽

Ariel Amadio ◽

...

Keyword(s):

Escherichia Coli ◽

High Throughput ◽

Enterohemorrhagic Escherichia Coli ◽

Escherichia Coli O157 ◽

Protein Abundance

Download Full-text

Antimicrobial Effect of Chemical Preservatives on Enterohemorrhagic Escherichia coli O157:H7.

JOURNAL OF HEALTH SCIENCE ◽

10.1248/jhs.46.204 ◽

2000 ◽

Vol 46 (3) ◽

pp. 204-208 ◽

Cited By ~ 7

Author(s):

Akiko Yamamura ◽

Aki Murai ◽

Hiromu Takamatsu ◽

Kazuhito Watabe

Keyword(s):

Escherichia Coli ◽

Antimicrobial Effect ◽

Enterohemorrhagic Escherichia Coli ◽

Escherichia Coli O157 ◽

Chemical Preservatives

Download Full-text

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

Journal of Veterinary Medical Science ◽

10.1292/jvms.66.585 ◽

2004 ◽

Vol 66 (5) ◽

pp. 585-587 ◽

Cited By ~ 14

Author(s):

Ayako EZAWA ◽

Fumie GOCHO ◽

Koji KAWATA ◽

Tatsufumi TAKAHASHI ◽

Naoya KIKUCHI

Keyword(s):

Escherichia Coli ◽

Enterohemorrhagic Escherichia Coli ◽

High Prevalence

Download Full-text

Polyester Cloth–Based Hybridization Array System for Identification of Enterohemorrhagic Escherichia coli Serogroups O26, O45, O103, O111, O121, O145, and O157

Journal of Food Protection ◽

10.4315/0362-028x.jfp-12-116 ◽

2012 ◽

Vol 75 (9) ◽

pp. 1691-1697 ◽

Cited By ~ 15

Author(s):

BURTON W. BLAIS ◽

MARTINE GAUTHIER ◽

MYLÈNE DESCHÊNES ◽

GEORGE HUSZCZYNSKI

Keyword(s):

Escherichia Coli ◽

Marker Gene ◽

Enterohemorrhagic Escherichia Coli ◽

Oligonucleotide Probes ◽

E Coli ◽

Polyester Cloth ◽

Gene Profiles ◽

Pcr Products ◽

Different Strains ◽

Immunoenzymatic Assay

A cloth-based hybridization array system (CHAS) was developed for the identification of foodborne colony isolates of seven priority enterohemorrhagic Escherichia coli (EHEC-7) serogroups targeted by U.S. food inspection programs. Gene sequences associated with intimin; Shiga-like toxins 1 and 2; and the antigenic markers O26, O45, O103, O111, O121, O145, and O157 were amplified in a multiplex PCR incorporating a digoxigenin label, and detected by hybridization of the PCR products with an array of specific oligonucleotide probes immobilized on a polyester cloth support, with subsequent immunoenzymatic assay of the captured amplicons. The EHEC-7 CHAS exhibited 100% inclusivity and 100% exclusivity characteristics with respect to detection of the various markers among 89 different E. coli strains, with various marker gene profiles and 15 different strains of non–E. coli bacteria.

Download Full-text

Two Enteropathogenic Escherichia coli Type III Secreted Proteins, EspA and EspB, Are Virulence Factors

Journal of Experimental Medicine ◽

10.1084/jem.188.10.1907 ◽

1998 ◽

Vol 188 (10) ◽

pp. 1907-1916 ◽

Cited By ~ 91

Author(s):

Akio Abe ◽

Ursula Heczko ◽

Richard G. Hegele ◽

B. Brett Finlay

Keyword(s):

Escherichia Coli ◽

Virulence Factors ◽

Enterohemorrhagic Escherichia Coli ◽

Laser Scanning Microscopy ◽

Secreted Proteins ◽

Confocal Laser ◽

Infection Model ◽

Enteropathogenic Escherichia Coli ◽

Type Iii

Enteropathogenic Escherichia coli (EPEC) belongs to a family of related bacterial pathogens, including enterohemorrhagic Escherichia coli (EHEC) O157:H7 and other human and animal diarrheagenic pathogens that form attaching and effacing (A/E) lesions on host epithelial surfaces. Bacterial secreted Esp proteins and a type III secretion system are conserved among these pathogens and trigger host cell signal transduction pathways and cytoskeletal rearrangements, and mediate intimate bacterial adherence to epithelial cell surfaces in vitro. However, their role in pathogenesis is still unclear. To investigate the role of Esp proteins in disease, mutations in espA and espB were constructed in rabbit EPEC serotype O103 and infection characteristics were compared to that of the wild-type strain using histology, scanning and transmission electron microscopy, and confocal laser scanning microscopy in a weaned rabbit infection model. The virulence of EspA and EspB mutant strains was severely attenuated. Additionally, neither mutant strain formed A/E lesions, nor did either one cause cytoskeletal actin rearrangements beneath the attached bacteria in the rabbit intestine. Collectively, this study shows for the first time that the type III secreted proteins EspA and EspB are needed to form A/E lesions in vivo and are indeed virulence factors. It also confirms the role of A/E lesions in disease processes.

Download Full-text