scholarly journals Low-cost optofluidic add-on enables rapid selective plane illumination microscopy of C. elegans with a conventional wide-field microscope

2021 ◽  
Vol 26 (12) ◽  
Author(s):  
Mehran Behrouzi ◽  
Khaled Youssef ◽  
Pouya Rezai ◽  
Nima Tabatabaei
Keyword(s):  
Low Cost ◽  
Wide Field ◽  
C Elegans ◽  
Selective Plane Illumination Microscopy

scholarly journals Process Emulation System for High-Power Piezoelectric Ultrasonic Actuators

2015 ◽  
Vol 2 (3-4) ◽  
pp. 201-205
Author(s):  
Igor Ille ◽  
Sebastian Mojrzisch ◽  
Jens Twiefel
Keyword(s):  
Feedback Control ◽  
Low Cost ◽  
Stable Process ◽  
Present System ◽  
Wide Field ◽  
Hardware In The Loop ◽  
Ultrasonic Assisted ◽  
Long Time ◽  
Number Of Cycles ◽  
High Flexibility

Abstract Ultrasonic actuators are used for a wide field of applications. The vibration energy can be used to realize many processes like ultrasonic welding or bonding. Furthermore there are many processes which run more efficient and faster combined with ultrasonic vibration like ultrasonic-assisted turning or drilling. Piezoelectric transducers are the main part of those applications. Most of the applications have a time-variant load behavior and need an amplitude feedback control to guarantee a stable process. To ensure correct function tests of the feedback control systems have to be done. In this case the processes have to be executed in association with a high number of cycles. To emulate the behavior of the environment the automotive and aerospace industries use hardware in the loop systems since a long time but there is no such a method for ultrasonic systems. This paper presents a method to realize high dynamic load emulation for different ultrasonic applications. Using a piezoelectric transformer it is possible to reproduce load curves by active damping on the secondary side of the transformer using a current proportional digital feedback circuit. A theoretical and experimental study of hardware in the loop system for ultrasonic applications is given by this paper. The present system allows testing a wide field of feedback control algorithms with high flexibility and a high number of cycles by utilization of low-cost components. This proceeding decreases design periods in association with feedback control.

Processing Panorama Video in Real-time

2014 ◽  
Vol 08 (02) ◽  
pp. 209-227 ◽  
Author(s):  
Håkon Kvale Stensland ◽  
Vamsidhar Reddy Gaddam ◽  
Marius Tennøe ◽  
Espen Helgedagsrud ◽  
Mikkel Næss ◽  
...  
Keyword(s):  
Real Time ◽  
Graphics Processing Units ◽  
Low Cost ◽  
Video Camera ◽  
Wide Field ◽  
High Data ◽  
Data Rates ◽  
Camera Arrays ◽  
Analysis System ◽  
Performance Results

There are many scenarios where high resolution, wide field of view video is useful. Such panorama video may be generated using camera arrays where the feeds from multiple cameras pointing at different parts of the captured area are stitched together. However, processing the different steps of a panorama video pipeline in real-time is challenging due to the high data rates and the stringent timeliness requirements. In our research, we use panorama video in a sport analysis system called Bagadus. This system is deployed at Alfheim stadium in Tromsø, and due to live usage, the video events must be generated in real-time. In this paper, we describe our real-time panorama system built using a low-cost CCD HD video camera array. We describe how we have implemented different components and evaluated alternatives. The performance results from experiments ran on commodity hardware with and without co-processors like graphics processing units (GPUs) show that the entire pipeline is able to run in real-time.

scholarly journals Mask R-CNN Based C. Elegans Detection with a DIY Microscope

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 257
Author(s):  
Sebastian Fudickar ◽  
Eike Jannik Nustede ◽  
Eike Dreyer ◽  
Julia Bornhorst
Keyword(s):  
Cell Biology ◽  
High Throughput Screening ◽  
Low Cost ◽  
Image Acquisition ◽  
Rapid Development ◽  
Model Organism ◽  
Large Data ◽  
Data Set ◽  
C Elegans ◽  
Do It Yourself

Caenorhabditis elegans (C. elegans) is an important model organism for studying molecular genetics, developmental biology, neuroscience, and cell biology. Advantages of the model organism include its rapid development and aging, easy cultivation, and genetic tractability. C. elegans has been proven to be a well-suited model to study toxicity with identified toxic compounds closely matching those observed in mammals. For phenotypic screening, especially the worm number and the locomotion are of central importance. Traditional methods such as human counting or analyzing high-resolution microscope images are time-consuming and rather low throughput. The article explores the feasibility of low-cost, low-resolution do-it-yourself microscopes for image acquisition and automated evaluation by deep learning methods to reduce cost and allow high-throughput screening strategies. An image acquisition system is proposed within these constraints and used to create a large data-set of whole Petri dishes containing C. elegans. By utilizing the object detection framework Mask R-CNN, the nematodes are located, classified, and their contours predicted. The system has a precision of 0.96 and a recall of 0.956, resulting in an F1-Score of 0.958. Considering only correctly located C. elegans with an [email protected] IoU, the system achieved an average precision of 0.902 and a corresponding F1 Score of 0.906.

Transfer and tissue-specific accumulation of components in embryos of Caenorhabditis elegans and dolichura: in vivo analysis with a low-cost signal

Development ◽  
1992 ◽  
Vol 114 (2) ◽  
pp. 317-330 ◽  
Author(s):  
O. Bossinger ◽  
E. Schierenberg
Keyword(s):  
Caenorhabditis Elegans ◽  
Low Cost ◽  
Free Living ◽  
Tissue Specific ◽  
C Elegans ◽  
Specific Accumulation ◽  
In Vivo Analysis

The pattern of autofluorescence in the two free-living namatodes Rhabditis dolichura and Caenorhabditis compared. In C. elegans, during later embryogenesis cells develop a typical bluish autofluorescence as illumination, while in Rh. dolichura a strong already present in the unfertilized egg. Using a new,

Synthesis of Electrocatalysts for Electrochemistry in Energy

2018 ◽  
pp. 300-385
Author(s):  
Olga Marquez ◽  
Jairo Márquez P
Keyword(s):  
Low Cost ◽  
Green Energy ◽  
Wide Field ◽  
Best Effort ◽  
Working Together

The increasing population demands clean and green energy, encouraging scientists and technologists to make their best effort to develop renewable, available, and low-cost acquisition of non-conventional energy. Researchers in Catalysis and Electrochemistry, working together, have reached good achievements when focused in electrochemistry studies that are under development for alternative, renewable, capture, conversion, storage, supply, uses, and applications of energy. This is called Electrochemistry in energy. The symbiosis Electrochemistry-Catalysis is fundamental in this field for successful results. Important achievements are nowadays found in literature and some of them are reported here with emphasis in the use of electrochemistry for electrosynthesis of the named photoelectrocatalysts. Thus, photoelectrocatalysts, photocatalysts, and catalysts are of importance in many of the aspects involved in the term Electrochemistry in energy. This is such a wide field, with many aspects presented here, that the authors give an appropriate view and pedagogical standpoint for the readers.

scholarly journals A Large Reflective Schmidt Telescope for Antarctica

2005 ◽  
Vol 13 ◽  
pp. 958-959
Author(s):  
Will Saunders ◽  
Andrew McGrath
Keyword(s):  
Low Cost ◽  
Wide Field ◽  
Simple Design ◽  
Schmidt Telescope ◽  
Fixed Axis

AbstractWe present a simple design for a 16 metre, wide-field, fixed-axis, all-reflective, low cost f/4 Schmidt telescope to take advantage of the unique advantages of Antarctica as an Optical/IR site.

scholarly journals Quantitative Analysis of Drug Efficacy on C. elegans Models for Neuromuscular Diseases

2021 ◽  
Author(s):  
Samuel Sofela ◽  
Sarah Sahloul ◽  
Yong-Ak Song
Keyword(s):  
Drug Screening ◽  
Low Cost ◽  
Neuromuscular Diseases ◽  
Model Organism ◽  
Drug Efficacy ◽  
Two Dimensional ◽  
C Elegans ◽  
Drug Candidates ◽  
High Throughput Drug Screening ◽  
Screening Assays

AbstractCaenorhabditis elegans has emerged as a powerful model organism for drug screening due to its cellular simplicity, genetic amenability and homology to humans combined with its small size and low cost. Currently, high-throughput drug screening assays are mostly based on image-based phenotyping not exploiting key locomotory parameters of this multicellular model with muscles such as its thrashing force, a critical parameter when screening drugs for muscle-related diseases. In this study, we demonstrated the use of a micropillar-based force assay chip in combination with an imaging assay to evaluate the efficacy of various drugs currently used in treatment of neuromuscular diseases. Using this two-dimensional approach, we showed that the force assay was generally more sensitive in measuring efficacy of drug treatment in Duchenne Muscular Dystrophy and Parkinson’s Disease mutant worms as well as partly in Amyotrophic Lateral Sclerosis model. These results underline the potential of our force assay chip in screening of potential drug candidates for the treatment of neuromuscular diseases when combined with an imaging assay in a two-dimensional analysis approach.

scholarly journals Clinical applications of OCTA in ocular oncology: pearls and pitfalls

2021 ◽  
Author(s):  
Marco Pellegrini ◽  
Giovanni Staurenghi ◽  
Chiara Preziosa
Keyword(s):  
Low Cost ◽  
Masking Effect ◽  
Wide Field ◽  
Imaging Tool ◽  
Intraocular Tumors ◽  
Malignant Lesions ◽  
Choroidal Vasculature ◽  
Media Opacities ◽  
Evaluation And Monitoring

Background: Optical Coherence Tomography Angiography (OCTA) is a valuable imaging tool for the diagnosis of several retinal and choroidal diseases. Its role in ocular oncology is clinically promising but still controversial. In this review we report the main applications and limits of the use of OCTA for the study of intraocular tumors. Summary: OCTA allows a rapid, safe, low-cost and high-resolution visualization of the retinal and choroidal vasculature. Attempts have been made to use this technology in ocular oncology to differentiate benign and malignant lesions and to assist physicians in the evaluation and monitoring of post-treatment complications. Main limitations include failure in correct segmentation due to tumor inner profile or thickness, poor penetration of laser into the lesion, masking effect from overlying fluid and media opacities and poor fixation. Key messages: The main applications of OCTA in ocular oncology consist in the documentation of tumor-associated choroidal neovascularizations and the study of vascular changes following tumor treatments. In particular, the diffusion of wide-field protocols makes OCTA suitable for the diagnosis and follow-up of radiation chorio-retinopathy allowing a detailed visualoization of both macular and peripheral ischemic changes. Optimistically, future innovations in OCTA technology may offer new perspectives in the diagnosis and follow-up of intraocular tumors.

scholarly journals A novel device for non-invasive cerebral perfusion assessment

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Mirko Tessari ◽  
Anna Maria Malagoni ◽  
Maria Elena Vannini ◽  
Paolo Zamboni
Keyword(s):  
Near Infrared ◽  
Single Photon ◽  
Human Subjects ◽  
Low Cost ◽  
Photon Emission ◽  
Diagnostic Technique ◽  
Brain Perfusion ◽  
Emission Computed Tomography ◽  
Wide Field ◽  
Non Invasive

Currently brain perfusion can be assessed by the means of radio-invasive methods, such as single-photon emission computed tomography and positron emission tomography, or by hightech methods such as magnetic resonance imaging. These methods are known to be very expensive, with long examination time, and finally, cannot be used for assessing brain oxygen distribution in relation to exercise and/or cognition-tests. The near infrared spectroscopy (NIRS) is a non-invasive diagnostic technique. In real time it is capable of measuring tissue oxygenation using portable instrumentation with a relative low cost. We and other groups previously adopted this instrument for investigation of the oxygen consumption in the muscles at rest and during exercise. NIRS can be now used to assess brain perfusion through the intact skull in human subjects by detecting changes in blood hemoglobin concentrations. Changes in perfusion can be related to both arterial and venous problems. This novel equipment features allow for a wide field of innovative applications where portability, wearability, and a small footprint are essential. The present review shows how to use it in relation to exercise protocols of the upper and lower extremities, measured in healthy people and in conditions of arterial and chronic cerebro-spinal venous insufficiency.

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