Management of all three phases of wound healing through the induction of fluorescence biomodulation using fluorescence light energy

Background: The ultimate goal of wound healing following minor injury is to form a tissue regenerate that has functionality and visual appearance as close to the original skin as possible. The body’s physiological response to any wound is traditionally characterised by three distinct steps: inflammation, proliferation and remodelling. Summary: New insights suggest that the three phases overlap (and even occur in parallel) in both time and space in the wound, necessitating a clinical approach that targets each stage simultaneously to ensure rapid repair and wound closure without further complications. Ingredients that exhibit activity across each of the three phases, such as dexpanthenol, are of value in the context of minor wound care and scar management. Key Messages: In addition to treatment and ingredient selection, it is also important to consider broader clinical best practices and self-care options that can be used to optimise the management of minor wounds. An individualised approach that can account for a patient’s unique requirements and preferences is critical in achieving effective wound recovery.

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Evaluation of Fluorescent Light Energy for the Treatment of Acute Second-degree Burns

Military Medicine ◽

10.1093/milmed/usaa299 ◽

2021 ◽

Vol 186 (Supplement_1) ◽

pp. 416-423

Author(s):

Maiken Mellergaard ◽

Stéphane Fauverghe ◽

Carlotta Scarpa ◽

Vladimir Luca Pozner ◽

Søren Skov ◽

...

Keyword(s):

Wound Healing ◽

Human Skin ◽

Ex Vivo ◽

Visible Spectrum ◽

Skin Inflammation ◽

Light Energy ◽

Fluorescent Light ◽

Skin Model ◽

Randomized Controlled Clinical Trials ◽

Second Degree

ABSTRACT Introduction The use of photobiomodulation has been proposed to improve wound healing for the last two decades. Recent development in photobiomodulation has led to the development of a novel biophotonic platform that utilizes fluorescent light energy (FLE) within the visible spectrum of light for healing of skin inflammation and wounds. Materials and Methods In this article, FLE was used in preliminary analysis on 18 case studies of acute second-degree burns and in a pilot study using an ex vivo human skin model. Efficacy of FLE on wound healing and tissue remodeling was evaluated by monitoring improvements in the treated tissues, assessing pain for the patients, and by performing human genome microarray analysis of FLE-treated human skin samples. Results Healing was reported for all 18 patients treated with FLE for acute second-degree burns without reported adverse effects or development of infections. Furthermore, preliminary ex vivo skin model data suggest that FLE impacts different cellular pathways including essential immune-modulatory mechanisms. Conclusions The results presented in this article are encouraging and suggest that FLE balances different stages of wound healing, which opens the door to initiating randomized controlled clinical trials for establishing the efficacy of FLE treatment in different phases of wound healing of second-degree burns.

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Epidemiology of Potato Gangrene in Tasmania

Australian Journal of Botany ◽

10.1071/bt9760337 ◽

1976 ◽

Vol 24 (3) ◽

pp. 337 ◽

Cited By ~ 5

Author(s):

RR Walker ◽

GC Wade

Keyword(s):

Wound Healing ◽

High Temperatures ◽

Fungal Isolate ◽

Potato Tubers ◽

Resistance Response ◽

Phoma Exigua ◽

Tuber Resistance ◽

Tuber Maturity ◽

Three Phases

In Tasmania, Phoma exigua Desm. var. exigua accounts for 90% of potential inoculum on tuber surfaces and 90% of gangrene lesions in stored potato tubers. P. exigua Desm, var, foveata (Foister) Boerema accounts for the remaining 10%. Tubers lifted from pre-senescent crops contain negligible levels of surface-borne inoculum and develop few lesions in storage. Washing removes only a small percentage of potential inoculum on tuber surfaces, which initiates infections if tubers are wounded and held in an environment unfavourable for wound healing. Tuber resistance to gangrene disease is determined by the interaction between tuber cultivar, tuber maturity and the nature of the fungal isolate within both varieties of P. exigua. The resistance response is greatest at high temperatures and is characterized by three phases: lesion retardation, lesion arrest and lesion rejection. Lesion rejection represents the end product of tuber resistance response and is associated with the development of post-infectional periderm.

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Fluorescent light energy modulates healing in skin grafted mouse model

Open Medicine ◽

10.1515/med-2021-0329 ◽

2021 ◽

Vol 16 (1) ◽

pp. 1240-1255

Author(s):

Jie Ding ◽

Maiken Mellergaard ◽

Zhensen Zhu ◽

Peter Kwan ◽

Deirdre Edge ◽

...

Keyword(s):

Wound Healing ◽

Mouse Model ◽

Chronic Wounds ◽

Skin Grafting ◽

Cell Types ◽

Tissue Growth ◽

Light Energy ◽

Fluorescent Light ◽

Collagen Remodeling ◽

Cell Accumulation

Abstract Skin grafting is often the only treatment for skin trauma when large areas of tissue are affected. This surgical intervention damages the deeper dermal layers of the skin with implications for wound healing and a risk of scar development. Photobiomodulation (PBM) therapy modulates biological processes in different tissues, with a positive effect on many cell types and pathways essential for wound healing. This study investigated the effect of fluorescent light energy (FLE) therapy, a novel type of PBM, on healing after skin grafting in a dermal fibrotic mouse model. Split-thickness human skin grafts were transplanted onto full-thickness excisional wounds on nude mice. Treated wounds were monitored, and excised xenografts were examined to assess healing and pathophysiological processes essential for developing chronic wounds or scarring. Results demonstrated that FLE treatment initially accelerated re-epithelialization and rete ridge formation, while later reduced neovascularization, collagen deposition, myofibroblast and mast cell accumulation, and connective tissue growth factor expression. While there was no visible difference in gross morphology, we found that FLE treatment promoted a balanced collagen remodeling. Collectively, these findings suggest that FLE has a conceivable effect at balancing healing after skin grafting, which reduces the risk of infections, chronic wound development, and fibrotic scarring.

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Management of canine perianal fistula with fluorescence light energy: preliminary findings

Veterinary Dermatology ◽

10.1111/vde.12890 ◽

2020 ◽

Vol 31 (6) ◽

pp. 460

Author(s):

Andrea Marchegiani ◽

Adolfo M. Tambella ◽

Alessandro Fruganti ◽

Andrea Spaterna ◽

Matteo Cerquetella ◽

...

Keyword(s):

Perianal Fistula ◽

Light Energy ◽

Fluorescence Light

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Fluorescent light energy in wound healing: when is a photon something more?

Mechanisms of Photobiomodulation Therapy XV ◽

10.1117/12.2546694 ◽

2020 ◽

Author(s):

Michela Zago ◽

Mehrnoush Dehghani ◽

Joanna Jaworska ◽

Maiken Mellergaard ◽

Dierdre Edge ◽

...

Keyword(s):

Wound Healing ◽

Light Energy ◽

Fluorescent Light

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ultrastructural process of intestinal wound healing

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141494 ◽

1995 ◽

Vol 53 ◽

pp. 1024-1025

Author(s):

Rick L. Vaughn ◽

Shailendra K. Saxena ◽

John G. Sharp

Keyword(s):

Wound Healing ◽

Epithelial Cells ◽

Smooth Muscles ◽

Microscopic Level ◽

Light Microscopic Level ◽

Ileal Mucosa ◽

Wound Model ◽

Serosal Surface ◽

Complex Process ◽

Orderly Fashion

We have developed an intestinal wound model that includes surgical construction of an ileo-cecal patch to study the complex process of intestinal wound healing. This allows approximation of ileal mucosa to the cecal serosa and facilitates regeneration of ileal mucosa onto the serosal surface of the cecum. The regeneration of ileal mucosa can then be evaluated at different times. The wound model also allows us to determine the rate of intestinal regeneration for a known size of intestinal wound and can be compared in different situations (e.g. with and without EGF and Peyer’s patches).At the light microscopic level it appeared that epithelial cells involved in regeneration of ileal mucosa originated from the enlarged crypts adjacent to the intestinal wound and migrated in an orderly fashion onto the serosal surface of the cecum. The migrating epithelial cells later formed crypts and villi by the process of invagination and evagination respectively. There were also signs of proliferation of smooth muscles underneath the migratory epithelial cells.

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Healing gone wrong: convergence of hemostatic pathways and liver fibrosis?

Clinical Science ◽

10.1042/cs20191102 ◽

2020 ◽

Vol 134 (16) ◽

pp. 2189-2201

Author(s):

Jessica P.E. Davis ◽

Stephen H. Caldwell

Keyword(s):

Wound Healing ◽

Liver Disease ◽

Hepatic Fibrosis ◽

Cell Activation ◽

Stellate Cell ◽

Factor Xa ◽

Clinical Trial Data ◽

Chronic Liver ◽

Healing Response ◽

Wound Healing Response

Abstract Fibrosis results from a disordered wound healing response within the liver with activated hepatic stellate cells laying down dense, collagen-rich extracellular matrix that eventually restricts liver hepatic synthetic function and causes increased sinusoidal resistance. The end result of progressive fibrosis, cirrhosis, is associated with significant morbidity and mortality as well as tremendous economic burden. Fibrosis can be conceptualized as an aberrant wound healing response analogous to a chronic ankle sprain that is driven by chronic liver injury commonly over decades. Two unique aspects of hepatic fibrosis – the chronic nature of insult required and the liver’s unique ability to regenerate – give an opportunity for pharmacologic intervention to stop or slow the pace of fibrosis in patients early in the course of their liver disease. Two potential biologic mechanisms link together hemostasis and fibrosis: focal parenchymal extinction and direct stellate cell activation by thrombin and Factor Xa. Available translational research further supports the role of thrombosis in fibrosis. In this review, we will summarize what is known about the convergence of hemostatic changes and hepatic fibrosis in chronic liver disease and present current preclinical and clinical data exploring the relationship between the two. We will also present clinical trial data that underscores the potential use of anticoagulant therapy as an antifibrotic factor in liver disease.

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