The optical properties (absorption and reduced scattering coefficients, µa and µs’) of bovine liver tissue for 635 nm has been determined by using integrating sphere and inverse adding-doubling (IAD) techniques. For this purpose, total reflectance and total transmittance values of bovine liver tissue sample, which is placed between two microscope slides, have been measured by using single-sphere system. The measured values have been used as input parameters for IAD program to extract the µa and µs’ of the sample. In this study, µa and µs’ of bovine liver tissue for 635 nm have been determined to be 0.22 mm-1 and 0.51mm-1, respectively. These values, which yield 1.44 mm penetration depth, are in good agreement with the ones in the literature.
Determination of the tumor tissue optical properties during and after photodynamic therapy using inverse Monte Carlo method and double integrating sphere between 350 and 1000 nm