Complete Nucleotide Sequence of KPC-3-Encoding Plasmid pKpQIL in the Epidemic Klebsiella pneumoniae Sequence Type 258

ABSTRACT We have determined the entire DNA sequence of plasmid pKpQIL, the bla KPC-3-carrying plasmid harbored by the carbapenem-resistant Klebsiella pneumoniae clone sequence type 258 (ST 258) in Israel. pKpQIL is a 113,637-bp, self-transmissible plasmid that belongs to the incompatibility group IncFII. It consists of a large backbone of a pKPN4-like plasmid and carries the bla KPC-3-containing Tn4401a transposon of a pNYC-like plasmid.

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Complete Nucleotide Sequence of the IncN Plasmid Encoding IMP-6 and CTX-M-2 from Emerging Carbapenem-Resistant Enterobacteriaceae in Japan

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.04759-14 ◽

2014 ◽

Vol 59 (2) ◽

pp. 1356-1359 ◽

Cited By ~ 23

Author(s):

Shizuo Kayama ◽

Norifumi Shigemoto ◽

Ryuichi Kuwahara ◽

Kenshiro Oshima ◽

Hideki Hirakawa ◽

...

Keyword(s):

Multidrug Resistance ◽

Nucleotide Sequence ◽

Klebsiella Pneumoniae ◽

Dna Sequence ◽

Complete Nucleotide Sequence ◽

Carbapenem Resistance ◽

Content Type ◽

Carbapenem Resistant ◽

The Family ◽

Carbapenem Resistant Enterobacteriaceae

ABSTRACTWe have determined the DNA sequence ofKlebsiella pneumoniaemultidrug resistance plasmid pKPI-6, which is a self-transmissible IncN-type plasmid. pKPI-6 harboringblaIMP-6andblaCTX-M-2confers a stealth-type carbapenem resistance phenotype on members of the familyEnterobacteriaceaethat is not detectable with imipenem. pKPI-6 is already epidemic in Japan, favoring the dissemination of IMP-6 and CTX-M-2 in members of the familyEnterobacteriaceae.

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Complete Nucleotide Sequence of the First KPC-2- and SHV-12-Encoding IncX Plasmid, pKpS90, from Klebsiella pneumoniae

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01712-12 ◽

2012 ◽

Vol 57 (1) ◽

pp. 618-620 ◽

Cited By ~ 27

Author(s):

Najiby Kassis-Chikhani ◽

Lionel Frangeul ◽

Laurence Drieux ◽

Christian Sengelin ◽

Vincent Jarlier ◽

...

Keyword(s):

Nucleotide Sequence ◽

Klebsiella Pneumoniae ◽

Complete Nucleotide Sequence ◽

Sequence Type ◽

University Hospital ◽

Incompatibility Group ◽

Content Type

ABSTRACTWe report the complete nucleotide sequence of the pKpS90 plasmid, carrying theblaKPC-2andblaSHV-12genes. This plasmid was isolated from a sequence type 258 (ST258)Klebsiella pneumoniaestrain responsible for an outbreak in a French university hospital in 2009. pKpS90 is a 53,286-bp plasmid that belongs to the IncX incompatibility group. pKpS90 consists of a backbone from IncX plasmids, in which the KPC-2-encoding Tn4401transposon and ablaSHV-12-encoding region have been inserted.

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Sequence Type 273 Carbapenem-Resistant Klebsiella pneumoniae Carrying bla NDM-1 and bla IMP-4

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00160-18 ◽

2018 ◽

Vol 62 (6) ◽

Cited By ~ 4

Author(s):

Lu Liu ◽

Yu Feng ◽

Haiyan Long ◽

Alan McNally ◽

Zhiyong Zong

Keyword(s):

Klebsiella Pneumoniae ◽

Southeast Asia ◽

Human Blood ◽

Sequence Type ◽

Whole Genome Sequence ◽

Whole Genome ◽

Content Type ◽

Carbapenem Resistant ◽

Transmissible Plasmid ◽

Long Read

ABSTRACT A carbapenem-resistant Klebsiella pneumoniae isolate was recovered from human blood. Its whole-genome sequence was obtained using Illumina and long-read MinION sequencing. The strain belongs to sequence type 273 (ST273), which was found recently and caused an outbreak in Southeast Asia. It has two carbapenemase genes, bla NDM-1 (carried by an ST7 IncN self-transmissible plasmid) and bla IMP-4 (located on a self-transmissible IncHI5 plasmid). Non-KPC-producing ST237 may represent a lineage of carbapenem-resistant K. pneumoniae , which warrants further monitoring.

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Complete Sequence of ablaKPC-2-Harboring IncFIIK1Plasmid from a Klebsiella pneumoniae Sequence Type 258 Strain

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02332-12 ◽

2013 ◽

Vol 57 (3) ◽

pp. 1542-1545 ◽

Cited By ~ 37

Author(s):

Liang Chen ◽

Kalyan D. Chavda ◽

Roberto G. Melano ◽

Michael R. Jacobs ◽

Michael H. Levi ◽

...

Keyword(s):

New York ◽

New York City ◽

Nucleotide Sequence ◽

Klebsiella Pneumoniae ◽

York City ◽

Complete Sequence ◽

Sequence Type ◽

Content Type ◽

City Area ◽

Carbapenem Resistant

ABSTRACTWe report the nucleotide sequence of a novelblaKPC-2-harboring IncFIIK1plasmid, pBK32179, isolated from a carbapenem-resistantKlebsiella pneumoniaeST258 strain from a New York City patient. pBK32179 is 165 kb long, consists of a large backbone of pKPN3-like plasmid, and carries an 18.5-kbblaKPC-2-containing element that is highly similar to plasmid pKpQIL. pBK32179-like plasmids were identified in 8.3% of strains in a collection of 96K. pneumoniaeisolates from hospitals in the New York City area.

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Complete Nucleotide Sequence of pCTX-M360, an Intermediate Plasmid between pEL60 and pCTX-M3, from a Multidrug-Resistant Klebsiella pneumoniae Strain Isolated in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00032-09 ◽

2009 ◽

Vol 53 (12) ◽

pp. 5291-5293 ◽

Cited By ~ 17

Author(s):

Wen-han Zhu ◽

Lan Luo ◽

Jia-yi Wang ◽

Xiao-hong Zhuang ◽

Ling Zhong ◽

...

Keyword(s):

Sequence Analysis ◽

Nucleotide Sequence ◽

Klebsiella Pneumoniae ◽

Complete Nucleotide Sequence ◽

Multidrug Resistant ◽

Incompatibility Group ◽

Extended Spectrum

ABSTRACT In this work we report the characterization of plasmid pCTX-M360, isolated from a Klebsiella pneumoniae strain from China and encoding the CTX-M-3 extended-spectrum β-lactamase. Sequence analysis of pCTX-M360 revealed extensive similarity with pEL60 and pCTX-M3, two other enterobacterial plasmids of the IncL/M incompatibility group. Compared to pEL60, pCTX-M360 contains several insertions but lacks most of a 27-kb insert found in pCTX-M3, suggesting that it could be an evolutionary intermediate between pEL60 and pCTX-M3.

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Complete Nucleotide Sequence of an Escherichia coli Sequence Type 410 Strain Carrying bla NDM-5 on an IncF Multidrug Resistance Plasmid and bla OXA-181 on an IncX3 Plasmid

Genome Announcements ◽

10.1128/genomea.01542-17 ◽

2018 ◽

Vol 6 (5) ◽

Cited By ~ 14

Author(s):

Søren Overballe-Petersen ◽

Louise Roer ◽

Kim Ng ◽

Frank Hansen ◽

Ulrik S. Justesen ◽

...

Keyword(s):

Escherichia Coli ◽

Multidrug Resistance ◽

Nucleotide Sequence ◽

Resistance Genes ◽

Complete Nucleotide Sequence ◽

Sequence Type ◽

Nanopore Sequencing ◽

Incompatibility Group ◽

E Coli ◽

Resistance Plasmid

ABSTRACT Using Nanopore sequencing, we describe here the circular genome of an Escherichia coli sequence type 410 (ST410) strain with five closed plasmids. A large 111-kb incompatibility group F (IncF) plasmid harbored bla NDM-5 and 16 other resistance genes. A 51-kb IncX3 plasmid carried QnrS1 and bla OXA-181 . E. coli isolates with both bla NDM-5 and bla OXA-181 carbapenemases are rare.

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Colistin Resistance in Carbapenem-Resistant Klebsiella pneumoniae Mediated by Chromosomal Integration of Plasmid DNA

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00404-17 ◽

2017 ◽

Vol 61 (8) ◽

Cited By ~ 2

Author(s):

Astrid V. Cienfuegos-Gallet ◽

Liang Chen ◽

Barry N. Kreiswirth ◽

J. Natalia Jiménez

Keyword(s):

Klebsiella Pneumoniae ◽

Plasmid Dna ◽

Clonal Expansion ◽

Genetic Alterations ◽

Sequence Type ◽

Chromosomal Integration ◽

Colistin Resistance ◽

Content Type ◽

Carbapenem Resistant ◽

Single Locus Variant

ABSTRACT Here we describe the spread of colistin resistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae in Medellín, Colombia. Among 32 isolates collected between 2012 and 2014, 24 showed genetic alterations in mgrB. Nineteen isolates belonged to sequence type 512 (ST512) (or its single locus variant [SLV]) and harbored an 8.1-kb hsdMSR insertion corresponding to ISKpn25, indicating a clonal expansion of the resistant strain. The insertion region showed 100% identity to several plasmids, suggesting that the colistin resistance is mediated by chromosomal integration of plasmid DNA.

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Emergence of carbapenem-resistant NDM-1-producing Klebsiella pneumoniae high-risk sequence type 147 in a tertiary care hospital in Tenerife, Spain

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2019.04.014 ◽

2019 ◽

Vol 17 ◽

pp. 240-241 ◽

Cited By ~ 1

Author(s):

Ángeles Sampere ◽

Diego García Martínez de Artola ◽

Julia Alcoba Florez ◽

Eduardo Pérez Roth

Keyword(s):

High Risk ◽

Klebsiella Pneumoniae ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Sequence Type ◽

Care Hospital ◽

Carbapenem Resistant

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Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China

BMC Microbiology ◽

10.1186/s12866-019-1593-5 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 10

Author(s):

Jun Li ◽

Zi-Yan Huang ◽

Ting Yu ◽

Xiao-Yan Tao ◽

Yong-Mei Hu ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Capsular Polysaccharide ◽

Control Strategies ◽

Virulence Gene ◽

Sequence Type ◽

Pfge Typing ◽

Main Cluster ◽

Carbapenem Resistant ◽

Isolation And Characterization

Abstract Background The molecular characterization of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates is not well studied. Our goal was to investigate the molecular epidemiology of CR-hvKP strains that were isolated from a Chinese hospital. Results All clinical carbapenem-resistant K. pneumoniae (CR-KP) isolates were collected and identified from patient samples between 2014 and 2017 from a Chinese hospital. The samples were subjected to screening for CR-hvKP by string test and the detection of the aerobactin gene. CR-hvKP isolates were further confirmed through neutrophil phagocytosis and a mice lethality assay. The CR-hvKP isolates were investigated for their capsular genotyping, virulence gene profiles, and the expression of carbapenemase genes by PCR and DNA sequencing. Multilocus sequence type (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to exclude the homology of these isolates. Twenty strains were identified as CR-hvKP. These strains were resistant to imipenem and several other antibiotics, however, most were susceptible to amikacin. Notably, two isolates were not susceptible to tigecycline. Capsular polysaccharide synthesis genotyping revealed that 17 of the 20 CR-hvKP strains belonged to the K2 serotype, while the others belonged to serotypes other than K1, K2, K5, K20, and K57. The strains were found to be positive for 10 types of virulence genes and a variety of these genes coexisted in the same strain. Two carbapenemase genes were identified: blaKPC-2 (13/20) and blaNDM-1 (1/20). PFGE typing revealed eight clusters comprising isolates that belonged to MLST types ST25, ST11 and ST375, respectively. PFGE cluster A was identified as the main cluster, which included 11 isolates that belong to ST25 and mainly from ICU department. Conclusions Our findings suggest that hospital-acquired infections may contribute in part to the CR-hvKP strains identified in this study. It also suggests that ST25 CR-hvKP strain has a clonal distribution in our hospital. Therefore, effective surveillance and strict infection control strategies should be implemented to prevent outbreak by CR-hvKP strains in hospitals setting.

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