scholarly journals Biochemical Analysis of Metallo-β-Lactamase NDM-3 from a Multidrug-Resistant Escherichia coli Strain Isolated in Japan

2014 ◽  
Vol 58 (6) ◽  
pp. 3538-3540 ◽  
Author(s):  
Tatsuya Tada ◽  
Tohru Miyoshi-Akiyama ◽  
Kayo Shimada ◽  
Teruo Kirikae
Keyword(s):  
Escherichia Coli ◽  
Biochemical Analysis ◽  
Multidrug Resistant ◽  
Coli Strain ◽  
Enzymatic Activities ◽  
New Delhi ◽  
Content Type ◽  
Genetic Context

ABSTRACTNew Delhi metallo-β-lactamase-3 (NDM-3) was identified in a multidrug-resistantEscherichia coliisolate, NCGM77, obtained from the feces of a patient in Japan. The enzymatic activities of NDM-3 against β-lactams were similar to those of NDM-1, although NDM-3 showed slightly lowerkcat/Kmratios for all the β-lactams tested except for doripenem. The genetic context forblaNDM-3wastnpA-blaNDM-3-bleMBL-trpF-dsbC-tnpA-sulI-qacEdeltaI-aadA2-dfrA1, which was present on an approximately 250-kb plasmid.

scholarly journals NDM-8 Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli Strain Isolated in Nepal

2013 ◽  
Vol 57 (5) ◽  
pp. 2394-2396 ◽  
Author(s):  
Tatsuya Tada ◽  
Tohru Miyoshi-Akiyama ◽  
Rajan K. Dahal ◽  
Manoj K. Sah ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Respiratory Tract ◽  
Multidrug Resistant ◽  
Coli Strain ◽  
Enzymatic Activities ◽  
Content Type

ABSTRACTA novel metallo-β-lactamase, NDM-8, was identified in a multidrug-resistantEscherichia coliisolate, IOMTU11 (NCGM37), obtained from the respiratory tract of a patient in Nepal. The amino acid sequence of NDM-8 has substitutions at positions 130 (Asp to Gly) and 154 (Met to Leu) compared with NDM-1. NDM-8 showed enzymatic activities against β-lactams similar to those of NDM-1.

scholarly journals Complete Circular Genome Sequence of a Multidrug-Resistant Escherichia coli Strain from Cuba Obtained with Nanopore and Illumina Hybrid Assembly

2019 ◽  
Vol 8 (48) ◽  
Author(s):  
Rosa Elena Hernández-Fillor ◽  
Michael Brilhante ◽  
Ivette Espinosa ◽  
Vincent Perreten
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Antibiotic Resistance Genes ◽  
Generation Cephalosporin ◽  
Multidrug Resistant ◽  
Coli Strain ◽  
Hybrid Assembly ◽  
Content Type ◽  
Long Reads ◽  
Cephalosporin Resistance

The complete genome sequence of a multidrug-resistant Escherichia coli strain isolated from a healthy pig in Cuba was determined using short and long reads. This strain carried four plasmids, including a 42,683-kb IncX1 plasmid, which contains the third-generation cephalosporin resistance gene bla CTX-M-32 together with other disinfectant and antibiotic resistance genes.

scholarly journals Detection of an Escherichia coli Sequence Type 167 Strain with Two Tandem Copies ofblaNDM-1in the Chromosome

2016 ◽  
Vol 55 (1) ◽  
pp. 199-205 ◽  
Author(s):  
Ping Shen ◽  
Maoli Yi ◽  
Ying Fu ◽  
Zhi Ruan ◽  
Xiaoxing Du ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Insertion Sequence ◽  
Southern Blotting ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
New Delhi ◽  
Genomic Context ◽  
Content Type ◽  
Link Type ◽  
Reference Genomes

ABSTRACTNew Delhi metallo-β-lactamase-1 (NDM-1)-producingEnterobacteriaceaehas disseminated rapidly throughout the world and poses an urgent threat to public health. Previous studies confirmed that theblaNDM-1gene is typically carried in plasmids but rarely in chromosome. We discovered a multidrug-resistantEscherichia colistrain Y5, originating from a urine sample and containing theblaNDM-1gene, which did not transfer by either conjugation or electrotransformation. We confirmed the possibility of its chromosome location by S1-pulsed-field gel electrophoresis (PFGE) and XbaI-PFGE, followed by Southern blotting. To determine the genomic background ofblaNDM-1, the genome of Y5 was completely sequenced and compared to other reference genomes. The results of our study revealed that this isolate consists of a 4.8-Mbp chromosome and three plasmids, it is an epidemic clone of sequence type (ST) 167, and it shows 99% identity withEscherichia coli6409 (GenBank accession no.CP010371), which lacks the sameblaNDM-1gene-surrounding structure as Y5. TheblaNDM-1gene is embedded in the chromosome along with two tandem copies of an insertion sequence common region 1 (ISCR1) element (sul1-ARR-3-cat-blaNDM-1-bleo-ISCR1), which appears intact in the plasmid fromProteus mirabilis(GenBank accession no.KP662515). The genomic context indicates that the ISCR1element mediated theblaNDM-1transposition from a single source plasmid to the chromosome. Our study is the first report of anEnterobacteriaceaestrain harboring a chromosomally integratedblaNDM-1, which directly reveals the vertical spreading pattern of the gene. Close surveillance is urgently needed to monitor the emergence and potential spread of ST167 strains that harborblaNDM-1.

scholarly journals Identification of a Novel NDM Variant, NDM-13, from a Multidrug-Resistant Escherichia coli Clinical Isolate in Nepal

2015 ◽  
Vol 59 (9) ◽  
pp. 5847-5850 ◽  
Author(s):  
Basudha Shrestha ◽  
Tatsuya Tada ◽  
Tohru Miyoshi-Akiyama ◽  
Kayo Shimada ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enzymatic Activity ◽  
Clinical Isolate ◽  
Multidrug Resistant ◽  
New Delhi ◽  
Content Type ◽  
Genetic Environment ◽  
Carbapenem Resistant

ABSTRACTA novel New Delhi metallo-β-lactamase, NDM-13, was identified in a carbapenem-resistantEscherichia coliclinical isolate obtained from the urine of a patient in Nepal. The enzymatic activity of NDM-13 against β-lactams was similar to that of NDM-1. However, NDM-13 displayed significantly higherkcat/Kmratios for cefotaxime. The genetic environment ofblaNDM-13was determined to betnpA-IS30-blaNDM-13-bleMBL-trpF-dsbC-cutA-groES-groL, withblaNDM-13located within the chromosome.

scholarly journals Draft Genome Sequence of the First New Delhi Metallo-β-Lactamase (NDM-1)-Producing Escherichia coli Strain Isolated in Peru

2018 ◽  
Vol 6 (13) ◽  
Author(s):  
Jesus Tamariz ◽  
Carlos Llanos ◽  
Carlos Seas ◽  
Paola Montenegro ◽  
Jose Lagos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Draft Genome ◽  
Coli Strain ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
New Delhi ◽  
Content Type ◽  
Clinically Significant

ABSTRACT We present here the draft genome sequence of the first New Delhi metallo-β-lactamase (NDM-1)-producing Escherichia coli strain, belonging to sequence type 155 (ST155), isolated in Peru. Assembly of this draft genome resulted in 5,061,184 bp, revealing a clinically significant resistome for β-lactams, aminoglycosides, tetracyclines, phenicols, sulfonamides, trimethoprim, and fluoroquinolones.

scholarly journals NDM-12, a Novel New Delhi Metallo-β-Lactamase Variant from a Carbapenem-Resistant Escherichia coli Clinical Isolate in Nepal

2014 ◽  
Vol 58 (10) ◽  
pp. 6302-6305 ◽  
Author(s):  
Tatsuya Tada ◽  
Basudha Shrestha ◽  
Tohru Miyoshi-Akiyama ◽  
Kayo Shimada ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Clinical Isolate ◽  
Urine Sample ◽  
Enzymatic Activities ◽  
Amino Acid Substitutions ◽  
New Delhi ◽  
Content Type ◽  
Carbapenem Resistant

ABSTRACTA novel New Delhi metallo-β-lactamase variant, NDM-12, was identified in a carbapenem-resistantEscherichia coliclinical isolate obtained from a urine sample from a patient in Nepal. NDM-12 differed from NDM-1 by two amino acid substitutions (M154L and G222D). The enzymatic activities of NDM-12 against β-lactams were similar to those of NDM-1, although NDM-12 showed lowerkcat/Kmratios for all β-lactams tested except doripenem. TheblaNDM-12gene was located in a plasmid of 160 kb.

scholarly journals NDM-5 Carbapenemase-Encoding Gene in Multidrug-Resistant Clinical Isolates of Escherichia coli from Algeria

2014 ◽  
Vol 58 (9) ◽  
pp. 5606-5608 ◽  
Author(s):  
Asma Sassi ◽  
Lotfi Loucif ◽  
Sushim Kumar Gupta ◽  
Mazouz Dekhil ◽  
Houria Chettibi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Clinical Isolates ◽  
New Delhi ◽  
Content Type ◽  
Encoding Gene ◽  
Blood Specimens

ABSTRACTHere, we report the first autochthonous cases of infections caused byblaNDM-5New Delhi metallo-β-lactamase-producingEscherichia colistrains recovered from urine and blood specimens of three patients from Algeria between January 2012 and February 2013. The three isolates belong to sequence type 2659 and they coexpressblaCTX-M-15with theblaTEM-1andblaaadA2genes.

scholarly journals Plasmid-Mediated Novel blaNDM-17 Gene Encoding a Carbapenemase with Enhanced Activity in a Sequence Type 48 Escherichia coli Strain

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Zhihai Liu ◽  
Yang Wang ◽  
Timothy R. Walsh ◽  
Dejun Liu ◽  
Zhangqi Shen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Treatment Options ◽  
Coli Strain ◽  
Sequence Type ◽  
Animal Origin ◽  
New Delhi ◽  
Gene Encoding ◽  
Content Type ◽  
Carbapenem Resistant

ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) have spread worldwide, leaving very few treatment options available. New Delhi metallo-beta-lactamase (NDM) is the main carbapenemase mediating CRE resistance and is of increasing concern. NDM-positive Enterobacteriaceae of human origin are frequently identified; however, the emergence of NDM, and particularly novel variants, in bacteria of food animal origin has never been reported. Here, we characterize a novel NDM variant (assigned NDM-17) identified in a β-lactam-resistant sequence type 48 (ST48) Escherichia coli strain that was isolated from a chicken in China. Compared to NDM-1, NDM-17 had three amino acid substitutions (V88L, M154L, and E170K) that confer significantly enhanced carbapenemase activity. Compared to NDM-5, NDM-17 had only one amino acid substitution (E170K) and slightly increased isolate resistance to carbapenem, as indicated by increased MIC values. The gene encoding NDM-17 (bla NDM-17) was located on an IncX3 plasmid, which was readily transferrable to recipient E. coli strain J53 by conjugation, suggesting the possibility of the rapid dissemination of bla NDM-17. Enzyme kinetics showed that NDM-17 could hydrolyze all β-lactams tested, except for aztreonam, and had a significantly higher affinity for all β-lactams tested than did NDM-5. The emergence of this novel NDM variant could pose a threat to public health because of its transferability and enhanced carbapenemase activity.

scholarly journals A Novel Variant, NDM-5, of the New Delhi Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli ST648 Isolate Recovered from a Patient in the United Kingdom

2011 ◽  
Vol 55 (12) ◽  
pp. 5952-5954 ◽  
Author(s):  
Michael Hornsey ◽  
Lynette Phee ◽  
David W. Wareham
Keyword(s):  
Escherichia Coli ◽  
United Kingdom ◽  
Multidrug Resistant ◽  
New Delhi ◽  
Content Type ◽  
Native Promoter ◽  
The United Kingdom ◽  
New Variant ◽  
Novel Variant ◽  
History Of

ABSTRACTA new variant of the New Delhi metallo-enzyme (NDM) carbapenemase was identified in a multidrug-resistantEscherichia coliST648 isolate recovered from the perineum and throat of a patient in the United Kingdom with a recent history of hospitalization in India. NDM-5 differed from existing enzymes due to substitutions at positions 88 (Val→Leu) and 154 (Met→Leu) and reduced the susceptibility ofE. coliTOP10 transformants to expanded-spectrum cephalosporins and carbapenems when expressed under its native promoter.

scholarly journals Complete Genome Assembly of a Multidrug-Resistant New Delhi Metallo-β-Lactamase 1 (NDM-1)-Producing Escherichia coli Human Isolate from a New Zealand Hospital

2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Catrina Olivera ◽  
Jasna Rakonjac
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
New Zealand ◽  
Resistance Genes ◽  
Complete Genome ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
New Delhi ◽  
Content Type ◽  
History Of

ABSTRACT We report the complete genome of a multidrug-resistant Escherichia coli strain isolated from a New Zealand patient with a history of hospitalization in India. The strain, carrying eight plasmids, harbors chromosome-encoded nfsA and nfsB mutations, which cause nitrofuran resistance, and class C β-lactamase (blaEC) and plasmid-encoded blaNDM-1, blaCTX-M-15, and blaCMY-6, as well as other antibiotic resistance genes.

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