scholarly journals Emergence of KPC-2-Producing Pseudomonas aeruginosa Sequence Type 463 Isolates in Hangzhou, China

2015 ◽  
Vol 59 (5) ◽  
pp. 2914-2917 ◽  
Author(s):  
Yan-yan Hu ◽  
Dan-xia Gu ◽  
Jia-chang Cai ◽  
Hong-wei Zhou ◽  
Rong Zhang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Molecular Epidemiology ◽  
Clonal Expansion ◽  
Sequence Type ◽  
Environment Analysis ◽  
Content Type ◽  
Genetic Environment ◽  
High Level ◽  
Level Resistance

ABSTRACTThirty-nineKlebsiella pneumoniaecarbapenemase (KPC)-producingPseudomonas aeruginosaisolates, all exhibiting high-level resistance to carbapenems and other β-lactam antibiotics, were isolated in Hangzhou, China. Molecular epidemiology analysis indicated the presence of two dominant clones, namely, clones A and B, both of which belong to sequence type 463 (ST463). A genetic environment analysis demonstrated that both clones harbor an ISKpn8transposase,blaKPC-2, and an ISKpn6-like transposase. These findings depict the features of clonal expansion and transmission of KPC-2-producingP. aeruginosastrains in Hangzhou, China.

scholarly journals Draft Genome Sequence of a Pseudomonas aeruginosa Sequence Type 3351 Strain Exhibiting High-Level Resistance to Polymyxins in a Pediatric Patient with Cystic Fibrosis in Mexico

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Roberto Rosales-Reyes ◽  
Fernanda Esposito ◽  
Bruna Fuga ◽  
Louise Cerdeira ◽  
Catalina Gayosso-Vázquez ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Pediatric Patient ◽  
Genome Sequence ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Content Type ◽  
High Level ◽  
Level Resistance

Here, we present the draft genome sequence of a Pseudomonas aeruginosa isolate (strain CF16053) belonging to a novel sequence type (ST), ST3351, isolated from a pediatric patient with cystic fibrosis (CF). CF16053 shows high-level resistance to polymyxins associated with mutations in the pmrB gene. Biofilm, pyoverdine, exotoxin A, and type III secretion system (T3SS) genes were identified.

scholarly journals First Outbreak of a Plasmid-Mediated Carbapenem-Hydrolyzing OXA-48 β-Lactamase in Klebsiella pneumoniae in Spain

2011 ◽  
Vol 55 (9) ◽  
pp. 4398-4401 ◽  
Author(s):  
Cristina Pitart ◽  
Mar Solé ◽  
Ignasi Roca ◽  
Anna Fàbrega ◽  
Jordi Vila ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Conjugative Plasmid ◽  
Content Type ◽  
Low Level ◽  
High Level ◽  
Level Resistance

ABSTRACTTwentyKlebsiella pneumoniaeisolates producing OXA-48 were collected from April 2009 to September 2010. Strains were clonally related and coproduced a CTX-M-15 β-lactamase. A conjugative plasmid of circa 70 kb carryingblaOXA-48was identified. Eleven isolates showed low-level resistance to carbapenems, whereas nine showed high-level resistance. Decreased expression of OmpK36 was related to high-level resistance to carbapenems. The isolates belonged to sequence type 101 (ST101). This is the first outbreak caused by an OXA-48-producingK. pneumoniaestrain in Spain.

scholarly journals Colistin Resistance in Carbapenem-Resistant Klebsiella pneumoniae Mediated by Chromosomal Integration of Plasmid DNA

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Astrid V. Cienfuegos-Gallet ◽  
Liang Chen ◽  
Barry N. Kreiswirth ◽  
J. Natalia Jiménez
Keyword(s):  
Klebsiella Pneumoniae ◽  
Plasmid Dna ◽  
Clonal Expansion ◽  
Genetic Alterations ◽  
Sequence Type ◽  
Chromosomal Integration ◽  
Colistin Resistance ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Single Locus Variant

ABSTRACT Here we describe the spread of colistin resistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae in Medellín, Colombia. Among 32 isolates collected between 2012 and 2014, 24 showed genetic alterations in mgrB. Nineteen isolates belonged to sequence type 512 (ST512) (or its single locus variant [SLV]) and harbored an 8.1-kb hsdMSR insertion corresponding to ISKpn25, indicating a clonal expansion of the resistant strain. The insertion region showed 100% identity to several plasmids, suggesting that the colistin resistance is mediated by chromosomal integration of plasmid DNA.

scholarly journals Outbreak Caused by NDM-1- and RmtB-Producing Escherichia coli in Bulgaria

2014 ◽  
Vol 58 (4) ◽  
pp. 2472-2474 ◽  
Author(s):  
Laurent Poirel ◽  
Encho Savov ◽  
Arzu Nazli ◽  
Angelina Trifonova ◽  
Iva Todorova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
The World ◽  
High Level ◽  
16S Rrna Methylase ◽  
Level Resistance

ABSTRACTTwelve consecutive carbapenem-resistantEscherichia coliisolates were recovered from patients (infection or colonization) hospitalized between March and September 2012 in different units at a hospital in Bulgaria. They all produced the carbapenemase NDM-1 and the extended-spectrum-β-lactamase CTX-M-15, together with the 16S rRNA methylase RmtB, conferring high-level resistance to all aminoglycosides. All those isolates were clonally related and belonged to the same sequence type, ST101. In addition to being the first to identify NDM-producing isolates in Bulgaria, this is the very first study reporting an outbreak of NDM-1-producingE. coliin the world.

scholarly journals Molecular Epidemiology of KPC-Producing Klebsiella pneumoniae Isolates in the United States: Clonal Expansion of Multilocus Sequence Type 258

2009 ◽  
Vol 53 (8) ◽  
pp. 3365-3370 ◽  
Author(s):  
Brandon Kitchel ◽  
J. Kamile Rasheed ◽  
Jean B. Patel ◽  
Arjun Srinivasan ◽  
Shiri Navon-Venezia ◽  
...  
Keyword(s):  
United States ◽  
Klebsiella Pneumoniae ◽  
Molecular Epidemiology ◽  
Plasmid Dna ◽  
Restriction Analysis ◽  
Clonal Expansion ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Sequence Type ◽  
Control And Prevention

ABSTRACT Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have become more common in the United States and throughout the world. We used pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to examine the molecular epidemiology of KPC-producing K. pneumoniae isolates sent to the Centers for Disease Control and Prevention (CDC) for reference testing from 1996 to 2008. A dominant strain, sequence type 258 (ST 258), was found and likely accounts for 70% of the CDC's K. pneumoniae PFGE database. Isolates with PFGE patterns related to ST 258 were identified in 10 of the 19 U.S. states currently reporting KPC-producing K. pneumoniae, in addition to one isolate from Israel. KPC subtyping and analysis of the surrounding genetic environment were subsequently performed on 23 representative isolates. Thirteen isolates identified as ST 258 possessed either bla KPC-2 or bla KPC-3 and some variability in the Tn4401 element upstream of the bla KPC gene. Escherichia coli DH10B was successfully transformed by electroporation with KPC-encoding plasmid DNA from 20 of the 23 isolates. Restriction analysis of plasmid DNA prepared from transformants revealed a diversity of band patterns, suggesting the presence of different plasmids harboring the bla KPC gene, even among isolates of the same ST.

scholarly journals High-Level Pacidamycin Resistance in Pseudomonas aeruginosa Is Mediated by an Opp Oligopeptide Permease Encoded by theopp-fabIOperon

2013 ◽  
Vol 57 (11) ◽  
pp. 5565-5571 ◽  
Author(s):  
Anita Mistry ◽  
Mark S. Warren ◽  
John K. Cusick ◽  
RoxAnn R. Karkhoff-Schweizer ◽  
Olga Lomovskaya ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Frequency ◽  
Cross Resistance ◽  
Peptide Antibiotics ◽  
Content Type ◽  
Resistant Mutants ◽  
High Level ◽  
Level Resistance ◽  
Resistance Mutants

ABSTRACTPacidamycins (or uridyl peptide antibiotics) possess selectivein vivoactivity againstPseudomonas aeruginosa. An important limitation for the therapeutic use of pacidamycins withP. aeruginosais the high frequency (10−6to 10−7) at which resistant mutants emerge. To elucidate the mechanism(s) of this resistance, pacidamycin-resistantP. aeruginosamutants were isolated. Two types of mutants were obtained. Type 1, or high-level resistance mutants with a pacidamycin MIC of 512 μg/ml, were more abundant, with a frequency of ∼2 × 10−6, and did not show cross-resistance with other antibiotics. Type 2, low-level resistance mutants, were isolated with a frequency of ∼10−8and had a pacidamycin MIC of 64 μg/ml (the MIC for the wild-type strain was 4 to 16 μg/ml). These mutants were cross-resistant to levofloxacin, tetracycline, and erythromycin and were shown to overexpress either the MexAB-OprM or MexCD-OprJ multidrug resistance efflux pumps. High-level resistant mutants were isolated by transposon mutagenesis and one insertion was localized tooppB, one of two periplasmic binding protein components of an oligopeptide transport system which is encoded by theopp-fabIoperon. The Opp system is required for uptake of pacidamycin across the inner membrane, since variousopp, but notfabI, mutants were resistant to high levels of pacidamycin. Both of the two putative Opp periplasmic binding proteins, OppA and OppB, were required for pacidamycin uptake. Although both impaired uptake into and efflux from the cell can cause pacidamycin resistance inP. aeruginosa, our data suggest that impaired uptake is the primary reason for the high-frequency and high-level pacidamycin resistance.

scholarly journals Molecular Epidemiology and Mechanisms of High-Level Resistance to Meropenem and Imipenem in Pseudomonas aeruginosa

2020 ◽  
Vol Volume 13 ◽  
pp. 285-293 ◽  
Author(s):  
Noha Anwar Hassuna ◽  
Marwa K Darwish ◽  
Mohamed Sayed ◽  
Reham Aly Ibrahem
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology ◽  
High Level ◽  
Level Resistance

scholarly journals First Report of Klebsiella oxytoca Strain Coproducing KPC-2 and IMP-8 Carbapenemases

2011 ◽  
Vol 55 (6) ◽  
pp. 2937-2941 ◽  
Author(s):  
Bin Li ◽  
Jing-Yong Sun ◽  
Qing-Zhong Liu ◽  
Li-Zhong Han ◽  
Xin-Hong Huang ◽  
...  
Keyword(s):  
Southern Hybridization ◽  
Klebsiella Oxytoca ◽  
Content Type ◽  
Genetic Environment ◽  
Carbapenem Resistant ◽  
Plasmid Analysis ◽  
High Level ◽  
Lactamase Gene ◽  
First Time ◽  
Level Resistance

ABSTRACTThe study shows for the first time the presence of theKlebsiella oxytocastrain fp10 coproducing plasmid-mediated KPC-2 and IMP-8 carbapenemases. The strain was obtained from the fecal sample of an inpatient and showed high-level resistance to imipenem and ertapenem (MICs > 32 μg/ml). Conjugation experiments demonstrated the transferability of the carbapenem-resistant determinants. The results of plasmid analysis and Southern hybridization revealed that theblaKPC-2gene was located on transferable plasmid pFP10-1 (∼54 kb), whereas theblaIMP-8gene was on transferable plasmid pFP10-2 (∼180 kb). Analysis of the genetic environment of these two genes has demonstrated that ISKpn6and ISKpn8are involved in the spread of theblaKPC-2gene, while the transposable elements IS26,intI1, andtniCmight contribute to the dissemination of theblaIMP-8gene. The chimera of several transposon-associated elements indicated a novel genetic environment of IMP-type metallo-β-lactamase gene inEnterobacteriaceaefrom China.

scholarly journals Emergence of Escherichia coli Sequence Type ST131 Carrying both theblaGES-5andblaCTX-M-15Genes

2011 ◽  
Vol 55 (6) ◽  
pp. 2974-2975 ◽  
Author(s):  
Juwon Kim ◽  
Seong Geun Hong ◽  
Il Kwon Bae ◽  
Ji Roung Kang ◽  
Seok Hoon Jeong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
High Level ◽  
Level Resistance

ABSTRACTEscherichia coliclinical isolate BD07372 of sequence type ST131 recovered from a bed sore specimen exhibited high-level resistance to ceftazidime and cefotaxime but exhibited susceptibility to imipenem and meropenem. The isolate harbored two β-lactamase genes, theblaCTX-M-15gene carried by an ∼250-kbp plasmid carrying the FIA and FIC replicons and theblaGES-5gene carried by a class 1 integron in the chromosome.

scholarly journals High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Aurélie Jayol ◽  
Patrice Nordmann ◽  
Adrian Brink ◽  
Maria-Virginia Villegas ◽  
Véronique Dubois ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wild Type ◽  
Content Type ◽  
Resistance Phenotype ◽  
Two Component ◽  
Genes Encoding ◽  
High Level ◽  
Type Strains ◽  
Level Resistance

ABSTRACT Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.

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