scholarly journals First Outbreak of a Plasmid-Mediated Carbapenem-Hydrolyzing OXA-48 β-Lactamase in Klebsiella pneumoniae in Spain

2011 ◽  
Vol 55 (9) ◽  
pp. 4398-4401 ◽  
Author(s):  
Cristina Pitart ◽  
Mar Solé ◽  
Ignasi Roca ◽  
Anna Fàbrega ◽  
Jordi Vila ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Conjugative Plasmid ◽  
Content Type ◽  
Low Level ◽  
High Level ◽  
Level Resistance

ABSTRACTTwentyKlebsiella pneumoniaeisolates producing OXA-48 were collected from April 2009 to September 2010. Strains were clonally related and coproduced a CTX-M-15 β-lactamase. A conjugative plasmid of circa 70 kb carryingblaOXA-48was identified. Eleven isolates showed low-level resistance to carbapenems, whereas nine showed high-level resistance. Decreased expression of OmpK36 was related to high-level resistance to carbapenems. The isolates belonged to sequence type 101 (ST101). This is the first outbreak caused by an OXA-48-producingK. pneumoniaestrain in Spain.

scholarly journals Emergence of KPC-2-Producing Pseudomonas aeruginosa Sequence Type 463 Isolates in Hangzhou, China

2015 ◽  
Vol 59 (5) ◽  
pp. 2914-2917 ◽  
Author(s):  
Yan-yan Hu ◽  
Dan-xia Gu ◽  
Jia-chang Cai ◽  
Hong-wei Zhou ◽  
Rong Zhang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Molecular Epidemiology ◽  
Clonal Expansion ◽  
Sequence Type ◽  
Environment Analysis ◽  
Content Type ◽  
Genetic Environment ◽  
High Level ◽  
Level Resistance

ABSTRACTThirty-nineKlebsiella pneumoniaecarbapenemase (KPC)-producingPseudomonas aeruginosaisolates, all exhibiting high-level resistance to carbapenems and other β-lactam antibiotics, were isolated in Hangzhou, China. Molecular epidemiology analysis indicated the presence of two dominant clones, namely, clones A and B, both of which belong to sequence type 463 (ST463). A genetic environment analysis demonstrated that both clones harbor an ISKpn8transposase,blaKPC-2, and an ISKpn6-like transposase. These findings depict the features of clonal expansion and transmission of KPC-2-producingP. aeruginosastrains in Hangzhou, China.

scholarly journals Outbreak Caused by NDM-1- and RmtB-Producing Escherichia coli in Bulgaria

2014 ◽  
Vol 58 (4) ◽  
pp. 2472-2474 ◽  
Author(s):  
Laurent Poirel ◽  
Encho Savov ◽  
Arzu Nazli ◽  
Angelina Trifonova ◽  
Iva Todorova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
The World ◽  
High Level ◽  
16S Rrna Methylase ◽  
Level Resistance

ABSTRACTTwelve consecutive carbapenem-resistantEscherichia coliisolates were recovered from patients (infection or colonization) hospitalized between March and September 2012 in different units at a hospital in Bulgaria. They all produced the carbapenemase NDM-1 and the extended-spectrum-β-lactamase CTX-M-15, together with the 16S rRNA methylase RmtB, conferring high-level resistance to all aminoglycosides. All those isolates were clonally related and belonged to the same sequence type, ST101. In addition to being the first to identify NDM-producing isolates in Bulgaria, this is the very first study reporting an outbreak of NDM-1-producingE. coliin the world.

scholarly journals NDM-1-Producing Klebsiella pneumoniae Resistant to Colistin in a French Community Patient without History of Foreign Travel

2012 ◽  
Vol 56 (6) ◽  
pp. 3432-3434 ◽  
Author(s):  
Corinne Arpin ◽  
Patrick Noury ◽  
Delphine Boraud ◽  
Laure Coulange ◽  
Alain Manetti ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Conjugative Plasmid ◽  
Content Type ◽  
Foreign Travel ◽  
Carbapenem Resistant ◽  
History Of ◽  
Uncomplicated Cystitis ◽  
At Home

ABSTRACTA carbapenem-resistantKlebsiella pneumoniaestrain, Kp5196, was responsible for an uncomplicated cystitis in a patient living at home and without history of foreign travel. This isolate produced the metallocarbapenemase NDM-1 and was resistant to all antibiotics except tetracyclines and colistin. TheK. pneumoniaestrain belonged to sequence type ST15, andblaNDM-1was carried by a nontypeable conjugative plasmid. Two months later, a similar ST15 isolate, Kp5241, was present in the patient but was additionally colistin resistant.

scholarly journals Emergence of Escherichia coli Sequence Type ST131 Carrying both theblaGES-5andblaCTX-M-15Genes

2011 ◽  
Vol 55 (6) ◽  
pp. 2974-2975 ◽  
Author(s):  
Juwon Kim ◽  
Seong Geun Hong ◽  
Il Kwon Bae ◽  
Ji Roung Kang ◽  
Seok Hoon Jeong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
High Level ◽  
Level Resistance

ABSTRACTEscherichia coliclinical isolate BD07372 of sequence type ST131 recovered from a bed sore specimen exhibited high-level resistance to ceftazidime and cefotaxime but exhibited susceptibility to imipenem and meropenem. The isolate harbored two β-lactamase genes, theblaCTX-M-15gene carried by an ∼250-kbp plasmid carrying the FIA and FIC replicons and theblaGES-5gene carried by a class 1 integron in the chromosome.

scholarly journals High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Aurélie Jayol ◽  
Patrice Nordmann ◽  
Adrian Brink ◽  
Maria-Virginia Villegas ◽  
Véronique Dubois ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wild Type ◽  
Content Type ◽  
Resistance Phenotype ◽  
Two Component ◽  
Genes Encoding ◽  
High Level ◽  
Type Strains ◽  
Level Resistance

ABSTRACT Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.

scholarly journals Ceftobiprole- and Ceftaroline-Resistant Methicillin-Resistant Staphylococcus aureus

2015 ◽  
Vol 59 (5) ◽  
pp. 2960-2963 ◽  
Author(s):  
Liana C. Chan ◽  
Li Basuino ◽  
Binh Diep ◽  
Stephanie Hamilton ◽  
Som S. Chatterjee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Content Type ◽  
Low Level ◽  
Mrsa Strains ◽  
High Level ◽  
Level Resistance

ABSTRACTThe role ofmecAmutations in conferring resistance to ceftobiprole and ceftaroline, cephalosporins with anti-methicillin-resistantStaphylococcus aureus(MRSA) activity, was determined with MRSA strains COL and SF8300. The SF8300 ceftaroline-passaged mutant carried a singlemecAmutation, E447K (E-to-K change at position 447), and expressed low-level resistance. This mutation in COL conferred high-level resistance to ceftobiprole but only low-level resistance to ceftaroline. The COL ceftaroline-passaged mutant, which expressed high-level resistance to ceftobiprole and ceftaroline, had mutations inpbp2,pbp4, andgdpPbut notmecA.

scholarly journals Draft Genome Sequence of a Pseudomonas aeruginosa Sequence Type 3351 Strain Exhibiting High-Level Resistance to Polymyxins in a Pediatric Patient with Cystic Fibrosis in Mexico

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Roberto Rosales-Reyes ◽  
Fernanda Esposito ◽  
Bruna Fuga ◽  
Louise Cerdeira ◽  
Catalina Gayosso-Vázquez ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Pediatric Patient ◽  
Genome Sequence ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Content Type ◽  
High Level ◽  
Level Resistance

Here, we present the draft genome sequence of a Pseudomonas aeruginosa isolate (strain CF16053) belonging to a novel sequence type (ST), ST3351, isolated from a pediatric patient with cystic fibrosis (CF). CF16053 shows high-level resistance to polymyxins associated with mutations in the pmrB gene. Biofilm, pyoverdine, exotoxin A, and type III secretion system (T3SS) genes were identified.

scholarly journals Molecular Analysis of Polymyxin Resistance among Carbapenemase-Producing Klebsiella pneumoniae in Colombia

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 284
Author(s):  
 Elsa De La De La Cadena ◽  
María Fernanda Mojica ◽  
Juan Carlos García-Betancur ◽  
Tobías Manuel Appel ◽  
Jessica Porras ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Molecular Characteristics ◽  
Amino Acid Substitutions ◽  
Resistant Clone ◽  
Broth Microdilution ◽  
Carbapenem Resistant ◽  
High Level ◽  
Level Resistance

Polymyxin resistance in Klebsiella pneumoniae has been attributed to mutations in mgrB, phoPQ, pmrAB, and crrAB and to the presence of mcr plasmid-mediated genes. Herein, we describe the molecular characteristics of 24 polymyxin- and carbapenem-resistant K. pneumoniae isolates recovered from six Colombian cities between 2009 and 2019. Minimum inhibitory concentrations (MICs) to polymyxin were confirmed by broth microdilution, and whole-genome sequencing was performed to determine sequence type, resistome, and mutations in the genes related to polymyxin resistance, as well the presence of mcr. The results showed high-level resistance to polymyxin (MICs ≥ 4 μg/mL). blaKPC-3 was present in the majority of isolates (17/24; 71%), followed by blaKPC-2 (6/24; 25%) and blaNDM-1 (1/24; 4%). Most isolates belonged to the CG258 (17/24; 71%) and presented amino acid substitutions in PmrB (22/24; 92%) and CrrB (15/24; 63%); mutations in mgrB occurred in only five isolates (21%). Additional mutations in pmrA, crrA, and phoPQ nor any of the mcr resistance genes were identified. In conclusion, we found clonal dissemination of polymyxin and carbapenem-resistant K. pneumoniae isolates in Colombia, mainly associated with CG258 and blaKPC-3. Surveillance of this multidrug-resistant clone is warranted due to the limited therapeutic options for the treatment of carbapenem-resistant K. pneumoniae infections.

scholarly journals Emergence ofileS2-Carrying, Multidrug-Resistant Plasmids in Staphylococcus lugdunensis

2016 ◽  
Vol 60 (10) ◽  
pp. 6411-6414 ◽  
Author(s):  
Pak-Leung Ho ◽  
Melissa Chun-Jiao Liu ◽  
Kin-Hung Chow ◽  
Cindy Wing-Sze Tse ◽  
Wai-U Lo ◽  
...  
Keyword(s):  
Hong Kong ◽  
Multidrug Resistant ◽  
Staphylococcus Lugdunensis ◽  
Content Type ◽  
Low Level ◽  
Resistance Determinants ◽  
Mupirocin Resistance ◽  
High Level ◽  
Level Resistance

ABSTRACTOf 137Staphylococcus lugdunensisisolates collected from two nephrology centers in Hong Kong, 10 (7.3%) and 3 (2.2%) isolates had high-level and low-level mupirocin resistance, respectively. Isolates with high-level resistance contained the plasmid-mediatedileS2gene, while isolates with low-level resistance contained the mutation V588F within the chromosomalileSgene. All but one of theileS2-positive isolates belong to the predominating clone HKU1. Plasmids carrying theileS2gene were mosaic and also cocarry multiple other resistance determinants.

scholarly journals Impact of KPC Production and High-Level Meropenem Resistance on All-Cause Mortality of Ventilator-Associated Pneumonia in Association with Klebsiella pneumoniae

2020 ◽  
Vol 64 (6) ◽  
Author(s):  
Francisco Rivera-Espinar ◽  
Isabel Machuca ◽  
Rocio Tejero ◽  
Jorge Rodríguez ◽  
Ana Mula ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Retrospective Cohort ◽  
Cox Regression ◽  
University Hospital ◽  
Ventilator Associated Pneumonia ◽  
Content Type ◽  
Appropriate Treatment ◽  
All Cause Mortality ◽  
High Level ◽  
Level Resistance

ABSTRACT Carbapenemase-producing Enterobacterales and specifically Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-Kp) are rapidly spreading worldwide. The prognosis of ventilator-associated pneumonia (VAP) caused by KPC-Kp is not well known. Our study tries to assess whether ventilator-associated pneumonia caused by a KPC-Kp strain is associated with higher all-cause mortality than that caused by carbapenem-susceptible isolates. This is a retrospective cohort study of patients with VAP due to K. pneumoniae from a 35-bed polyvalent intensive care unit in a university hospital (>40,000 annual admissions) between January 2012 and December 2016. Adjusted multivariate analysis was used to study the association of KPC-Kp with 30-day all-cause mortality (Cox regression). We analyze 69 cases of K. pneumoniae VAP, of which 39 were produced by a KPC-Kp strain with high-level resistance to meropenem (MIC > 16 mg/ml). All-cause mortality at 30 days was 41% in the KPC-Kp group (16/39) and 33.3% in the carbapenem-susceptible cases (10/30). KPC-Kp etiology was not associated with higher mortality when controlled for confounders (adjusted hazard ratio [HR], 1.25; 95% confidence interval [CI], 0.46 to 3.41). Adequate targeted therapy (HR, 0.03; 95% CI, <0.01 to 0.23) was associated with all-cause mortality. Assuming the limitations due to the available sample size, the prognosis of VAP caused by KPC-Kp is similar to VAPs caused by carbapenem-susceptible K. pneumoniae when appropriate treatment is used.

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