scholarly journals Cation Components of Mueller-Hinton Agar Affecting Testing of Pseudomonas aeruginosa Susceptibility to Gentamicin

1980 ◽  
Vol 17 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Kenny ◽  
H. M. Pollock ◽  
B. H. Minshew ◽  
E. Casillas ◽  
F. D. Schoenknecht
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

scholarly journals Isolation, Identification And Prevalence Of Pseudomonas Aeruginosa Isolates From Clinical And Environmental Sources In Onitsha Metropolis, Anambra State

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
C. O. Ezeador ◽  
P. C. Ejikeugwu ◽  
S. N. Ushie ◽  
N. R. Agbakoba
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Prevalence Rate ◽  
Nasal Swab ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Anambra State ◽  
Mueller Hinton ◽  
Bluish Green ◽  
Mueller Hinton Agar

This study was aimed to isolate and identify Pseudomonas aeruginosa and to determine the prevalence rate of isolated P. aeruginosa in Hospitals in Onitsha. Isolates of P. aeruginosa were recovered from both clinical and environmental sources using Cetrimide agar, Blood agar, Mueller-Hinton agar and MacConkey agar.  All the inoculated plates were incubated at 37°C for 24-48 hours and growth was evaluated on these media. Isolates were identified on the basis of standard bacteriological methods like morphology, colonial characteristics, smell in culture, haemolysis, as well as pigment production on these media. All suspected isolates were further characterized and identified by many biochemical reactions. Results revealed that only 22 (18.3%) isolates were P. aeruginosa, while other 98 (81.7%) represented other bacterial genera. The 22 isolates included 19 (86.4%) environmental isolates and 3 (13.6%) clinical isolates. Pseudomonas aeruginosa was most commonly isolated from sink (13.6%), then mops and cleaning buckets (9.1%) and least from theatre bed, nasal swab, floor, disinfectant, ear and wound swab (4.5%). The pigment varied from bluish-green to yellowish-green with a grape-like odor. All isolates were Gram negative, produced β-hemolysis on blood agar and were motile. The biochemical tests showed all the isolates to be strongly positive for catalase, oxidase, citrate, and casein hydrolysis. The prevalence rate of P. aeruginosa is relatively high and its isolation from sources like sinks and theatre bed could be suggestive of the role of this pathogen in nosocomial infections.

scholarly journals Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro

2021 ◽  
Vol 10 (1) ◽  
pp. 16
Author(s):  
Saskia Arientika Wahyuningrum ◽  
Meiskha Bahar ◽  
Andri Pramesyanti Pramono
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
P Value ◽  
Gram Positive Bacteria ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
The One ◽  
One Way Anova

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa

scholarly journals Effect of zinc concentration in Mueller-Hinton agar on susceptibility of Pseudomonas aeruginosa to imipenem.

1997 ◽  
Vol 35 (4) ◽  
pp. 1027-1029 ◽  
Author(s):  
J S Daly ◽  
R A Dodge ◽  
R H Glew ◽  
D T Soja ◽  
B A DeLuca ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Zinc Concentration ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Prevalence, diversity and antimicrobial resistance of Salmonella enterica and Pseudomonas aeruginosa isolates from spring water in a rural area of northwestern Morocco

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Ilham Nassri ◽  
LATIFA TAHRI ◽  
AMAL SAIDI ◽  
NAJIA AMEUR ◽  
MOHAMMED FEKHAOUI
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Rural Area ◽  
Salmonella Enterica ◽  
Spring Water ◽  
Diffusion Method ◽  
Group Iii ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Abstract. Nassri I, Tahri L, Saidi A, Ameur N, Fekhaoui M. 2021. Prevalence, diversity and antimicrobial resistance of Salmonella enterica and Pseudomonas aeruginosa isolates from spring Water in a rural area of northwestern Morocco. Biodiversitas 22: 1363-1370. The persistence and diversity of serotypes belonging to pathogens in environmental waters including surface and groundwater have been widely documented and that the aquatic environment represents a relatively stable environment for these microorganisms. Study was carried out on the prevalence, diversity, and antibiotic resistance of Salmonella enterica and Pseudomonas aeruginosa isolates taken from 102 samples of spring water in a rural region of Northwestern Morocco (Rabat-Salé-Zemour-Zaer) collected for two seasonal campaigns between March 2010 and June 2011. The search and identification of S. enterica and P. aeruginosa were carried out according to ISO 19250 and ISO 16266 methods respectively. The serotyping of S. enterica and P. aeruginosa was carried out according to the Kauffmann and White and IATS (International Antigenic Typing System) schemes respectively. Antibiotic resistance of S. enterica and P. aeruginosa were carried out by the Mueller-Hinton agar diffusion method (Biorad). S. enterica showed a prevalence of 10.7% with 09 different serotypes circulating including S. paratyphi B, S. brandenburg, S. kentucky, S. group III b (serotype 50: z52: z53,), S. boon, S. tshiongwe, S. assinie, S. togo, and S. tanger. In contrast, P. aeruginosa showed a prevalence of 21.6% with 07 different serotypes circulating including O6, O1, O7, O9, O4, O3, and O10. In this study, antimicrobial resistance revealed that all isolated strains of S. enterica and P. aeruginosa still exhibit a wild resistance phenotype. Contaminated water sources are reservoirs of these pathogens but do not yet present the risk factors for these bacteria to develop antibiotic resistance.

scholarly journals The efficacy of Calendula officinalis tincture as an antibacterial on in vitro Pseudomonas aeruginosa

2002 ◽  
Author(s):  
◽  
Mbuso Mabuza
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Calendula Officinalis ◽  
Disc Diffusion Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Microbial Study

The aim of this in vitro microbial study was to evaluate the efficacy of Calendula officinalis tincture 60% (v/v) ethanol as an antibacterial on in vitro Pseudomonas aeruginosa. The standardised disc - diffusion method was employed. Seven pairs of Mueller - Hinton agar plates were used.

scholarly journals The peculiarities of Pseudomonas aeruginosa resistance to antibiotics and prevalence of serogroups

Medicina ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 36 ◽  
Author(s):  
Greta Gailienė ◽  
Alvydas Pavilonis ◽  
Violeta Kareivienė
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Laboratory ◽  
Multidrug Resistant ◽  
National Committee ◽  
Clinical Specimens ◽  
Resistance To Antibiotics ◽  
Microdilution Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Resistant Strains

Pseudomonas aeruginosa is one of the most common nonfermenting aerobic gramnegative microorganisms identified in clinical specimens of hospitalized patients. The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa strains is a growing concern in hospitalacquired infections. Typing of strains is important for identifying the sources of infection as well as prevention of cross-infections and monitoring of the efficacy of antimicrobial therapy. The aim of this study was to evaluate the antimicrobial resistance and prevalence of Pseudomonas aeruginosa serogroups isolated at Kaunas University of Medicine Hospital, Lithuania. Material and methods. Minimum inhibitory concentrations of piperacillin, cefoperazone, ceftazidime, cefotaxime, cefepime, imipenem, meropenem, gentamicin, amikacin, tobramycin, and ciprofloxacin for 609 Pseudomonas aeruginosa strains isolated from various clinical specimens between November 2001 and November 2002 were determined by the microdilution method in Mueller–Hinton agar using interpretative guidelines of National Committee for Clinical Laboratory Standards. Serogroups of Pseudomonas aeruginosa strains were identified using serums of Seiken Co. Ltd (Tokyo, Japan), containing antibodies against antigens of Pseudomonas aeruginosa O-group. Results. Pseudomonas aeruginosa strains were the most sensitive to ceftazidime (78.9%), imipenem (73.6%), meropenem (70.9%) and the most resistant to gentamicin (54.1%) and ciprofloxacin (52.5%). Multidrug-resistant strains made up 9.85% of all Pseudomonas aeruginosa strains investigated. Multidrug-resistant Pseudomonas aeruginosa strains were 1.5–3.5 times more resistant to antibiotics compared to non-multidrug-resistant strains, except to amikacin: multidrug-resistant strains were more sensitive (81.7%) than non-multidrug-resistant Pseudomonas aeruginosa strains (61.0%). Pseudomonas aeruginosa serogroups O:E and O:B were the most common serogroups (34.7% and 29.0%, respectively) followed by serogroups O:I (11.4%) and O:A (10.1%). Pseudomonas aeruginosa serogroup O:E strains were the most prevalent among multidrug-resistant strains (48.3%). Conclusions. The results of our study show that serogroup O:E was the most prevalent serogroup of Pseudomonas aeruginosa in our hospital, and its resistance to antibiotics was the highest.

scholarly journals Pseudomonas aeruginosa: an emerging nosocomial trouble in veterinary

2015 ◽  
pp. 4937-4946 ◽  
Author(s):  
Yuly Bernal-Rosas ◽  
Karen Osorio-Muñoz ◽  
Orlando Torres-García
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
Clinical Specimens ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Intermediate Response

ABSTRACT Objective. The goal of this study was to evaluate the susceptibility pattern of isolates P. aeruginosa from veterinary clinical centers in Bogotá, D.C., to some commonly used antibiotics in clinical. Materials and methods. Bacteriological standard protocols were used for the isolation and identification of bacterial strains. To evaluate the antimicrobial susceptibility of the isolates, to commonly used antibiotics, was performed the Kirby-Bauer agar-disk diffusion method on Mueller-Hinton agar. Results. A total of 160 samples was taken from clinical specimens and the environment in different veterinary clinics. Out of these samples, 89 (55.6%) were gram-negative strains, of which ten strains of P. aeruginosa were isolated (11.2%). All strains were resistant to Cefazolin, Lincomycin, Cephalothin, Ampicillin, Clindamycin, Sulfamethoxazole-Trimethoprim and Chloramphenicol while some isolates exhibited either resistance or an intermediate response to Amikacin (30%), Gentamicin (30%), Tobramycin (10%), Ciprofloxacin (20%), Ceftazidime (30%), Erythromycin (100%), Tetracycline (100%), Imipenem (10%), Meropenem (90%) and Bacitracin (90%). Conclusions. The results demonstrate that the acquired antimicrobial resistances of P. aeruginosa strains depend on antibiotic protocols applied. As observed in human hospitals, Pseudomonas aeruginosa is acting as one of the multidrug-resistant microorganisms of veterinary clinical relevance.

Sign in / Sign up

Export Citation Format

Share Document