scholarly journals Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro

2021 ◽  
Vol 10 (1) ◽  
pp. 16
Author(s):  
Saskia Arientika Wahyuningrum ◽  
Meiskha Bahar ◽  
Andri Pramesyanti Pramono
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
P Value ◽  
Gram Positive Bacteria ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
The One ◽  
One Way Anova

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa

scholarly journals Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

2021 ◽  
Vol 21 (1) ◽  
pp. 281
Author(s):  
Muhammad Zaidan Tsani Ariandi ◽  
Meiskha Bahar ◽  
Hany Yusmaini ◽  
Fajriati Zulfa ◽  
Cut Fauziah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
P Value ◽  
E Coli ◽  
Botanical Gardens

Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.  

scholarly journals The efficacy of Calendula officinalis tincture as an antibacterial on in vitro Pseudomonas aeruginosa

2002 ◽  
Author(s):  
◽  
Mbuso Mabuza
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Calendula Officinalis ◽  
Disc Diffusion Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Microbial Study

The aim of this in vitro microbial study was to evaluate the efficacy of Calendula officinalis tincture 60% (v/v) ethanol as an antibacterial on in vitro Pseudomonas aeruginosa. The standardised disc - diffusion method was employed. Seven pairs of Mueller - Hinton agar plates were used.

scholarly journals Antimicrobial Effect Of Honey Distributed Around Pasar Minggu Jakarta Selatan

2017 ◽  
Vol 8 (2) ◽  
pp. 101-106
Author(s):  
Ruth Elenora Kristanty ◽  
Junie Suriawati ◽  
Priyanto Dwi Nugroho
Keyword(s):  
Infectious Diseases ◽  
Food Product ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Bacteria Growth ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Almost All

Honey is a highly nutritious food product and consumed by almost all the population in the world. It has a function as an antimicrobial. Staphylococcus aureus (S. aureus) is a common Gram-positive bacteria in food and Escherichia coli (E. coli) is a Gram-negative bacteria that often appears in environmental sanitation issues that both can cause infectious diseases.  Some infectious diseases can be treated with antimicrobials such as honey. The purpose of this study was to test the antimicrobial effects on honey products distributed in Pasar Minggu area. The antimicrobial effect test was performed in vitro using agar diffusion method by measuring the inhibition zone formed where the bacteria growth was inhibited by the presence of sample. The concentration of samples were 25%, 50%, 75%, and 100% (not diluted) and as aquades control. The results showed that honey tested with various dilution concentrations resulted inhibition zone and. The higher concentration of the inhibited zone zone showed antimicrobial activity against S. aureus and E. coli.

scholarly journals Microwave-Assisted Synthesis, Structural Characterization and Assessment of the Antibacterial Activity of Some New Aminopyridine, Pyrrolidine, Piperidine and Morpholine Acetamides

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 533
Author(s):  
Abdulmajeed S. H. Alsamarrai ◽  
Saba S. Abdulghani
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Molecular Structures ◽  
Secondary Amines ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

A series of new acetamide derivatives 22–28 of primary and secondary amines and para-toluene sulphinate sodium salt have been synthesized under microwave irradiation and assessed in vitro for their antibacterial activity against one Gram-positive and two Gram-negative bacterial species such as S. pyogenes, E. coli, and P. mirabilis using the Mueller-Hinton Agar diffusion (well diffusion) method. The synthesized compounds with significant differences in inhibition diameters and MICs were compared with those of amoxicillin, ampicillin, cephalothin, azithromycin and doxycycline. All of the evaluated acetamide derivatives were used with varying inhibition concentrations of 6.25, 12.5, 37.5, 62.5, 87.5, 112.5 and 125 µg/mL. The results show that the most important antibacterial properties were displayed by the synthetic compounds 22 and 24, both of bear a para-chlorophenyl moiety incorporated into the 2-position moiety of acetamide 1. The molecular structures of the new compounds were determined using the FT-IR and 1H-NMR techniques.

scholarly journals Bacteriological Assessment and Antibiotics Susceptibility Profile of Bacteria Recovered from Pond Water, Fish Skin, and Gut in Ile-Ife, Osun State, Nigeria

2019 ◽  
Author(s):  
Cecilia Nireti Fakorede ◽  
Evelyn Nwadinkpa Fatokun ◽  
Blessing Philip Kantiok ◽  
Chinwe Juliana Iwu ◽  
Ishmael Festus Jaja
Keyword(s):  
Resistance Index ◽  
Pond Water ◽  
Quality Parameters ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Gram Positive Bacteria ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Antibiotics Susceptibility ◽  
Pond Type

Fish currently provide 6.7% of all protein consumed by humans globally, nevertheless, aquaculture system has been linked to fish and environmental contamination and disease outbreak. This study aims to isolate, identify, and characterise, bacteria in fish and pond water as well as the antibiotic profile of detected Coliforms. The susceptibility of the isolates was tested using the Kirby-Bauer disc diffusion method on Mueller Hinton agar. A total of forty (40) isolates were isolated from the water samples of which (5) species were Gram Positive bacteria and 35 species of Gram Negative bacteria. The temperature for all ponds ranged from 25&deg;C to 28&deg;C. The mean bacteria count for pond C1 to T2 were 4.9 &times; 102, 4.9 &times; 102, 5.4 &times; 102, 2.5 &times; 102, 2.2 &times; 102, and 1.9 &times; 102 CFU/ml respectively. All isolates were 100% resistant to ceftazidime, cefuroxime and augmentin. More resistance to cefixime (80%) and gentamicin (73.3%) and nitrofurantoin (66.7%) was recorded. However, only 16.6% and 8.3% of the isolates were resistant to ciprofloxacin and ofloxacin respectively. The multiple antimicrobial resistance index (MARI) ranged from 0.5 to 0.9. The water quality parameters (temperature and pH) and the type of bacteria detected in all pond type did not differ significantly. The Multi-drug resistance bacteria detected could be pathogenic to fish and consumers.

scholarly journals The potential of traditional balinese spices against the growth of Salmonella sp in vitro

2020 ◽  
Vol 9 (1) ◽  
pp. 121-127
Author(s):  
I Nyoman Jirna ◽  
I Gede Sudarmanto ◽  
Surya Bayu Kurniawan ◽  
Gusti Ayu Made Ratih ◽  
Burhannuddin Rasyid
Keyword(s):  
Control Design ◽  
Diffusion Method ◽  
Test Results ◽  
Antimicrobial Substances ◽  
Antimicrobial Potential ◽  
The One ◽  
Negative Controls ◽  
One Way Anova ◽  
Active Substances

Prevention by utilizing medicinal plants can be used as an alternative treatment. This study aims to identify active antimicrobial substances and test the antimicrobial potential of traditional Balinese spices, namely Basa Selem, Basa Gede, Basa Wangen, Basa Bawang Jahe, and Basa Rajang against Salmonella sp bacteria in vitro. The True-experimental method with Posttest only-control design was used in this study, by intervening in the treatment group as well as the presence of positive and negative controls. The maceration method with 96% EtOH solvent was used to extract active substances and identify the levels of antimicrobial active substances. The TLC Spectrophotodensitometer instrument and the diffusion method (discs) were used to test antimicrobial potential. Data analysis was performed using the one-way ANOVA test. All five samples showed flavonoids, alkaloids, tannins, and phenols in qualitative tests. Based on quantitative test results of five samples, the highest compound content obtained in Basa Wangen (6.66 mg/ml of tannins), Basa Gede  (3.74 mg/ml of flavonoids), Basa Bawang Jahe (2.49 mg/ml of tannins), Basa Selem (2.87 mg/ml of tannin), and Basa Rajang (6.96 mg/ml of flavonoids). There are differences in the antimicrobial potential of various types of traditional Balinese spices against the growth of Salmonella sp in vitro (sig = 0.037). The traditional Balinese spices have the antimicrobial potential of the intermediate category with a range of inhibition (16-20 mm) based on the NCCLS standard.

scholarly journals Microwave-assisted Synthesis, Structural Characterization of Amino Pyridines, Pyrrolidine, Piperidine, Morpholine, Acetamides, and Assessment of Their Antibacterial Activity

2020 ◽  
Author(s):  
Abdulmajeed Alsamarrai ◽  
Saba Abdulghani
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Molecular Structures ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
New Compounds

A sequence of new acetamide derivatives 9-15 of primary, secondary amine, and para-toluene sulphinate sodium salt have been synthesized under microwave irradiation and assessed in vitro for their antibacterial activity against one Gram-positive and two Gram-negative bacterial species such as S. pyogenes, E. coli, and P.mirabilis using the Mueller-Hinton Agar diffusion (well diffusion) method. The synthesized compounds with significant differences in inhibition diameters and MICs were compared with those of amoxicillin, ampicillin, cephalothin, azithromycin and doxycycline. All of the evaluated acetamide derivatives were used with varying inhibition concentrations of 6.25, 12.5, 37.5, 62.5, 87.5, 112.5 and 125 &micro;g/ml. The results show that the most important antibacterial properties exercised by the synthetic compounds 9 and 11 bearing para-chlorophenyl moiety incorporated into the 2-position moiety of acetamide 2. The molecular structures of the new compounds were determined using FT-IR, 1H-NMR techniques.

scholarly journals Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro

2021 ◽  
Vol 4 (1) ◽  
pp. 63
Author(s):  
Devi Indah Sari ◽  
Retno Sri Wahjuni ◽  
Ratih Novita Praja ◽  
Budi Utomo ◽  
Faisal Fikri ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Citrus Aurantifolia ◽  
E Coli ◽  
Randomized Design ◽  
Mueller Hinton ◽  
Lime Peel

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.

scholarly journals Comparison of Antimicrobial Activity of Fluoride Varnishes Against Streptococcusmutans and Lactobacillus acidophilus: An In Vitro Study

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Banafsheh Soleimani ◽  
Hamidreza Goli ◽  
Mahsa Naranjian ◽  
Seyed Jaber Mousavi ◽  
Azam Nahvi
Keyword(s):  
Lactobacillus Acidophilus ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Vitro Study ◽  
Diffusion Method ◽  
P Value ◽  
Antibacterial Effects ◽  
The Mean

Background: Topical fluoride products such as varnishes prevent dental caries by limiting demineralization and antibacterial properties. The structural and metabolic characteristics of cariogenic microorganisms are different from each other. Also, the formulation properties, concentration, and release behavior of fluoride vary in different varnishes. Objectives: In this study, we investigated the antibacterial effects of three types of common fluoride varnishes against two cariogenic bacteria of Streptococcus mutans (S.M.) and Lactobacillus acidophilus (L.A.). Methods: In this in vitro study, antibacterial effects of MI varnish [containing calcium phosphopeptide-amorphous calcium phosphate (CPP-ACP)], Polimo (containing xylitol), and FluoroDose varnishes were evaluated using disc diffusion method via measuring the diameter of the inhibition zone. The antibiotics of erythromycin and ampicillin were used as the positive control of the test. Analysis of variance (ANOVA) and post hoc tests were applied to compare the mean of non-growth zone diameter in the studied groups. Data were analyzed by SPSS 16. The statistical significance level was determined as P-value < 0.05. Results: The antibacterial effect of each varnish was optimal against both bacteria. In comparison, the mean diameter of the inhibition zone in MI varnish was significantly (P-value=0.019) higher than Polimo and FluoroDose brands on S.M.; however, this difference was not statistically significant for L.A. Furthermore, Polimo and FluoroDose varnishes showed similar antimicrobial effects against these bacteria. Conclusions: The use of these varnishes seems to be suitable for preventing tooth decay. MI varnish is preferable because of its higher antibacterial properties.

scholarly journals Daya Hambat Minyak Atsiri Rimpang Kunyit terhadap Pertumbuhan Candida Albicans In Vitro

2018 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Fitri Nadifah ◽  
Nurlaili Farida Muhajir ◽  
Fitri Retnoningsih
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Significant Difference ◽  
Oil Concentration ◽  
Anti Fungal ◽  
One Way Anova

Abstract: Inhibition Activity Of Turmeric Essential Oil Against The Growth Of Candida Albicans. Turmeric has rhizomes which contain essential oils and curcuminoid that has anti-fungal activity. The purpose of this research was to know the influence of essential oil of turmeric Rhizome (Curcuma domestica Val.) against the growth of Candida albicans in vitro. This was an experimental research with diffusion method. Essential oil obtained through distillation methods and the concentration used was 20%, 40%, 60%, 80% and 100%. The treatment was repeated three times. Anova test was used. Inhibition zone is formed at a concentration of 20%, 40%, 60%, 80%, 100% were 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm and 7.83 mm, respectively Results in analysis by one way ANOVA showed there was a significant difference in the variance of essential oil concentration against the inhibition zone (p< 0.05).Abstrak: Daya Hambat Minyak Atsiri Rimpang Kunyit Terhadap Pertumbuhan Candida Albicans In Vitro. Kunyit merupakan  salah satu rimpang yang diketahui memiliki kandungan minyak atsiri serta curcuminoid yang bersifat anti jamur. Tujuan penelitian ini untuk mengetahui pengaruh minyak atsiri rimpang kunyit (Curcuma domestica Val.) terhadap pertumbuhan Candida albicans secara in vitro. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi cara sumuran. Minyak atsiri didapatkan melalui   metode destilasi dan konsentrasi  yang digunakan adalah 20%, 40%, 60%, 80% dan 100%. Perlakuan diulang sebanyak tiga kali. Hasil penelitian di analisis dengan Anova. Hasil zona hambat yang terbentuk pada konsentrasi minyak atsiri 20%, 40%, 60%, 80%, 100% berturut-turut adalah 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm,  dan 7,83 mm. Hasil analisis dengan one way anova menunjukkan terdapat perbedaan yang signfikan pada berbagai konsentrasi minyak atsiri terhadap zona hambat yang dihasilkan (p<0,05). Dari penelitian ini dapat disimpulkan bahwa berbagai konsentrasi minyak atsiri rimpang kunyit dapat menghambat pertumbuhan Candida albicans. Kemampuan ini berpotensi dikembangkan lebih lanjut sebagai alternatif dalam pengobatan terhadap penyakit karena infeksi Candida albicans.

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